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Polr1b (NM_009086) Mouse Untagged Clone
CAT＃: MC223668
Polr1b (untagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b), (10ug)
CNY 14,540.00
货期*
6周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 128kDa; D630020H17Rik; RPA2; RPA116; RPA135; Rpo1-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223668 representing NM_009086
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTCGACGGCCGGTGGCGGAACCTGCCCAGCGGGCCCAGCCTAAAGCATTTAACCGACCCCTCGT ACGGGATTCCCCCAGAGCAGCAAAAGGCAGCTTTGCAGGACCTGACGCGGGCGCACGTGGACTCCTTCAA CTACGCGGCGCTGGAGGGGCTGAGCCACGCGGTGCAGGCCATACCTCCCTTTGAATTTGCTTTCAAAGAT GAGCGCATATCTCTTACCATTGTGGATGCTGTCATCAGTCCACCCTCAGTGCCCAAAGGGACCATCTGCA AAGATCTCAACGTTTATCCAGCTGAGTGTCGTGGCCGAAAGAGCACCTACCGAGGGAGGCTGACGGCTGA TATCAGCTGGGCCGTGAATGGAGTCCCCAAAGGGATCATTAAACAGTTTCTCGGCTATGTTCCCATCATG GTGAAGTCCAAGCTTTGCAACTTATACAACCTTCCTCCTCGAGTCCTCATTGAGCACCATGAGGAGGCAG AGGAAATGGGCGGTTATTTTATAATCAATGGCATAGAAAAAGTCATCCGCATGTTGATTGTGCCCCGGAG AAATTTCCCCATCGCCATGGTAAGGCCGAAATGGAAAAGCAGAGGACTCGGCTACACTCAGTTTGGGGTC TCCATGCGCTGTGTGAGAGAAGAGCACTCCGCCGTGAACATGAACCTGCACTATGTGGAGAACGGCACGG TTATGTTAAACTTTATTTACCGCAAAGAGCTCTTTTTCCTTCCTTTGGGATTTGCACTTAAGGCACTCGT GAGCTTTTCTGATTATCAGATTTTCCAGGAGCTTATCAAGGGCAAAGAGGAGGACTCTTTCTTTAGGAAT TCTGTTTCTCAGATGCTGCGGATTGTCATAGAAGAGGGCTGTCACTCACAGAAGCAGGTTCTTAACTATC TGGGAGAATGCTTCCGAGTGAAGCTCAGCCTTCCTGATTGGTACCCTAACGTGGAAGCTGCCGAGTTCCT GCTTAACCAGTGCATCTGCATCCACCTGCAGTCCAACACTGACAAGTTCTACCTGCTCTGCCTCATGACC CGGAAGCTGTTCGCTTTAGCCAGAGGGGAGTGCATGGACGACAACCCTGATAGCCTAGTGAACCAAGAGG TCCTCAGCCCTGGGCAGCTCTTCCTGATGTTTCTGAAGGAAAAAATGGAAAACTGGCTAGTGTCTATTAA AATAGTTTTAGATAAGAGGGCTCAGAAGGCCAATGTTTCCATAAACAACGAAAATTTGATGAAGATTTTT AGTATGGGAACAGAGCTAACAAGACCATTTGAATATCTTCTTGCTACTGGGAATCTGCGTTCTAAAACAG GTCTCGGCTTCCTGCAAGATTCTGGCCTGTGTGTTGTGGCTGACAAGCTGAACTTCCTTCGCTACCTCTC CCATTTCCGCTGTGTGCACAGAGGGGCTGCCTTTGCCAAGATGAGGACCACCACCGTGCGCAGGCTGCTG CCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACTCCAGACGGGGCGCCTTGTGGGCTGTTGAACCACT TGACTGCCGTGTGTGAGGTTGTTACCAAGTTTGTGTACACGGCATCTATTCCAGCCTTGCTCTGTGGCTT AGGAGTCACTCCTGTTGATACAGCGCCATGTCGACCGTACAGTGACTGCTACCCTGTCCTGCTGGATGGC GTCATGGTGGGCTGGGTGGATAAGGACCTTGCTCCTGAAGTTGCAGACACTCTCCGTCGATTCAAGGTGT TGAGAGAAAAAAGAATTCCTCCCTGGATGGAGGTGGCCCTGATTCCCATGACAGGAAAACCAAGTCTGTA CCCAGGTCTGTTCCTCTTTACCACTCCCTGCAGGCTGGTGAGGCCTGTGCAGAACTTGGAACTGGGCAGA GAAGAGCTCATTGGAACCATGGAACAGCTCTTCATGAACGTCGCCATCTTTGAGGACGAGGTGTTTGGTG GCATCTCCACACACCAGGAGCTCTTCCCTCACAGCCTGCTGAGCGTGATCGCCAACTTCATCCCCTTCTC GGATCACAACCAGAGTCCTCGGAACATGTACCAGTGCCAGATGGGTAAGCAGACCATGGGCTTTCCGCTG CTCACTTACCAAAACCGATCAGATAATAAACTCTATCGACTCCAAACCCCTCAGAGCCCTCTAGTGAGAC CGTGCATGTATGATTTCTATGACATGGACAACTATCCGATCGGGACAAACGCCATTGTAGCTGTGATCTC CTACACTGGCTATGATATGGAGGACGCCATGATTGTCAACAAGGCTTCCTGGGAACGAGGCTTCGCTCAT GGAAGCGTCTACAAGTCTGAGTTCATAGACCTCTCAGAGAAGTTTAAGCAGGGAGAGGATAACCTGGTGT TCGGGGTCAAGCCTGGTGACCCTCGGGTGATGCAGAAGCTGGACGATGATGGGTTGCCATTCATAGGCGC AAAGCTGGAGTATGGCGACCCTTACTACAGCTACCTCAACCTTAACACTGGGGAAGGCTTCGTGGTTTAC TACAAGAGTAAAGAAAACTGTGTTGTGGACAACATCAAAGTGTGTAGTAACGACATGGGCAGTGGGAAGT TCAAGTGTATCTGCATCACAGTCCGCATCCCCCGGAACCCAACCATTGGAGATAAGTTTGCCAGCCGACA TGGTCAGAAGGGCATTTTGAGCAGATTGTGGCCAGCCGAGGACATGCCTTTCACTGAGAGTGGCATGATG CCGGACATTCTGTTTAATCCTCACGGGTTTCCCTCCCGTATGACCATAGGTATGTTAATCGAGAGCATGG CTGGGAAGTCTGCAGCTTTGCACGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCCGAGGAGAACTC TGCCTTAGAGTACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAACTTCTACGGCACCGAGAGACTGTAT AGTGGCATCAGTGGGATGGAGCTGGAGGCTGACATCTTCATTGGTGTGGTTTATTACCAGCGCTTACGAC ACATGGTGTCAGACAAATTTCAAGTCAGAACAACTGGAGCCAGGGACAAAGTCACCAACCAGCCCCTCGG GGGACGAAATGTTCAGGGTGGAATCCGCTTTGGGGAGATGGAGCGGGATGCTCTGTTGGCACACGGTACG TCTTTCCTTCTTCATGACCGGCTTTTCAACTGCTCTGACCGTTCTGTCGCTCACATGTGTGTAGAGTGTG GCAGTTTGCTTTCTCCGCTACTTGAGAAGCCTCCTCCGTCTTGGTCTGCGATGCGCAACAGAAAGTACAA CTGCACTGTCTGTGGCCGCAGTGACACCATCGACACCGTCTCTGTGCCGTATGTCTTCCGGTACTTTGTA GCTGAGCTGGCAGCCATGAACATCAAAGTGAAACTGGATGTCATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009086 |
| Insert Size | 3408 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009086.2, NP_033112.2 |
| RefSeq Size | 4017 bp |
| RefSeq ORF | 3408 bp |
| Locus ID | 20017 |
| UniProt ID | P70700 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211691 | Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b) |
CNY 7,912.00 |
|
| MR211691L3 | Lenti ORF clone of Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b) |
CNY 10,260.00 |
|
| MR211691L4 | Lenti ORF clone of Polr1b (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b) |
CNY 10,260.00 |
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