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Polr1b (NM_009086) Mouse Tagged ORF Clone
CAT＃: MR211691
- TrueORF®
Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b)
"NM_009086" in other vectors (3)
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CNY 7,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 128kDa; D630020H17Rik; RPA2; RPA116; RPA135; Rpo1-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211691 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTCGACGGCCGGTGGCGGAACCTGCCCAGCGGGCCCAGCCTAAAGCATTTAACCGACCCCTCGT ACGGGATTCCCCCAGAGCAGCAAAAGGCAGCTTTGCAGGACCTGACGCGGGCGCACGTGGACTCCTTCAA CTACGCGGCGCTGGAGGGGCTGAGCCACGCGGTGCAGGCCATACCTCCCTTTGAATTTGCTTTCAAAGAT GAGCGCATATCTCTTACCATTGTGGATGCTGTCATCAGTCCACCCTCAGTGCCCAAAGGGACCATCTGCA AAGATCTCAACGTTTATCCAGCTGAGTGTCGTGGCCGAAAGAGCACCTACCGAGGGAGGCTGACGGCTGA TATCAGCTGGGCCGTGAATGGAGTCCCCAAAGGGATCATTAAACAGTTTCTCGGCTATGTTCCCATCATG GTGAAGTCCAAGCTTTGCAACTTATACAACCTTCCTCCTCGAGTCCTCATTGAGCACCATGAGGAGGCAG AGGAAATGGGCGGTTATTTTATAATCAATGGCATAGAAAAAGTCATCCGCATGTTGATTGTGCCCCGGAG AAATTTCCCCATCGCCATGGTAAGGCCGAAATGGAAAAGCAGAGGACTCGGCTACACTCAGTTTGGGGTC TCCATGCGCTGTGTGAGAGAAGAGCACTCCGCCGTGAACATGAACCTGCACTATGTGGAGAACGGCACGG TTATGTTAAACTTTATTTACCGCAAAGAGCTCTTTTTCCTTCCTTTGGGATTTGCACTTAAGGCACTCGT GAGCTTTTCTGATTATCAGATTTTCCAGGAGCTTATCAAGGGCAAAGAGGAGGACTCTTTCTTTAGGAAT TCTGTTTCTCAGATGCTGCGGATTGTCATAGAAGAGGGCTGTCACTCACAGAAGCAGGTTCTTAACTATC TGGGAGAATGCTTCCGAGTGAAGCTCAGCCTTCCTGATTGGTACCCTAACGTGGAAGCTGCCGAGTTCCT GCTTAACCAGTGCATCTGCATCCACCTGCAGTCCAACACTGACAAGTTCTACCTGCTCTGCCTCATGACC CGGAAGCTGTTCGCTTTAGCCAGAGGGGAGTGCATGGACGACAACCCTGATAGCCTAGTGAACCAAGAGG TCTTCAGCCCTGGGCAGCTCTTCCTGATGTTTCTGAAGGAAAAAATGGAAAACTGGCTAGTGTCTATTAA AATAGTTTTAGATAAGAGGGCTCAGAAGGCCAATGTTTCCATAAACAACGAAAATTTGATGAAGATTTTT AGTATGGGAACAGAGCTAACAAGACCATTTGAATATCTTCTTGCTACTGGGAATCTGCGTTCTAAAACAG GTCTCGGCTTCCTGCAAGATTCTGGCCTGTGTGTTGTGGCTGACAAGCTGAACTTCCTTCGCTACCTCTC CCATTTCCGCTGTGTGCACAGAGGGGCTGCCTTTGCCAAGATGAGGACCACCACCGTGCGCAGGCTGCTG CCAGAATCCTGGGGCTTCCTCTGCCCTGTGCACACTCCAGACGGGGCGCCTTGTGGGCTGTTGAACCACT TGACTGCCGTGTGTGAGGTTGTTACCAAGTTTGTGTACACGGCATCTATTCCAGCCTTGCTCTGTGGCTT AGGAGTCACTCCTGTTGATACAGCGCCATGTCGACCGTACAGTGACTGCTACCCTGTCCTGCTGGATGGC GTCATGGTGGGCTGGGTGGATAAGGACCTTGCTCCTGAAGTTGCAGACACTCTCCGTCGATTCAAGGTGT TGAGAGAAAAAAGAATTCCTCCCTGGATGGAGGTGGCCCTGATTCCCATGACAGGAAAACCAAGTCTGTA CCCAGGTCTGTTCCTCTTTACCACTCCCTGCAGGCTGGTGAGGCCTGTGCAGAACTTGGAACTGGGCAGA GAAGAGCTCATTGGAACCATGGAACAGCTCTTCATGAACGTCGCCATCTTTGAGGACGAGGTGTTTGGTG GCATCTCCACACACCAGGAGCTCTTCCCTCACAGCCTGCTGAGCGTGATCGCCAACTTCATCCCCTTCTC GGATCACAACCAGAGTCCTCGGAACATGTACCAGTGCCAGATGGGTAAGCAGACCATGGGCTTTCCGCTG CTCACTTACCAAAACCGATCAGATAATAAACTCTATCGACTCCAAACCCCTCAGAGCCCTCTAGTGAGAC CGTGCATGTATGATTTCTATGACATGGACAACTATCCGATCGGGACAAACGCCATTGTAGCTGTGATCTC CTACACTGGCTATGATATGGAGGACGCCATGATTGTCAACAAGGCTTCCTGGGAACGAGGCTTCGCTCAT GGAAGCGTCTACAAGTCTGAGTTCATAGACCTCTCAGAGAAGTTTAAGCAGGGAGAGGATAACCTGGTGT TCGGGGTCAAGCCTGGTGACCCTCGGGTGATGCAGAAGCTGGACGATGATGGGTTGCCATTCATAGGCGC AAAGCTGGAGTATGGCGACCCTTACTACAGCTACCTCAACCTTAACACTGGGGAAGGCTTCGTGGTTTAC TACAAGAGTAAAGAAAACTGTGTTGTGGACAACATCAAAGTGTGTAGTAACGACATGGGCAGTGGGAAGT TCAAGTGTATCTGCATCACAGTCCGCATCCCCCGGAACCCAACCATTGGAGATAAGTTTGCCAGCCGACA TGGTCAGAAGGGCATTTTGAGCAGATTGTGGCCAGCCGAGGACATGCCTTTCACTGAGAGTGGCATGATG CCGGACATTCTGTTTAATCCTCACGGGTTTCCCTCCCGTATGACCATAGGTATGTTAATCGAGAGCATGG CTGGGAAGTCTGCAGCTTTGCACGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCCGAGGAGAACTC TGCCTTAGAGTACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAACTTCTACGGCACCGAGAGACTGTAT AGTGGCATCAGTGGGATGGAGCTGGAGGCTGACATCTTCATTGGTGTGGTTTATTACCAGCGCTTACGAC ACATGGTGTCAGACAAATTTCAAGTCAGAACAACTGGAGCCAGGGACAAAGTCACCAACCAGCCCCTCGG GGGACGAAATGTTCAGGGTGGAATCCGCTTTGGGGAGATGGAGCGGGATGCTCTGTTGGCACACGGTACG TCTTTCCTTCTTCATGACCGGCTTTTCAACTGCTCTGACCGTTCTGTCGCTCACATGTGTGTAGAGTGTG GCAGTTTGCTTTCTCCGCTACTTGAGAAGCCTCCTCCGTCTTGGTCTGCGATGCGCAACAGAAAGTACAA CTGCACTGTCTGTGGCCGCAGTGACACCATCGACACCGTCTCTGTGCCGTATGTCTTCCGGTACTTTGTA GCTGAGCTGGCAGCCATGAACATCAAAGTGAAACTGGATGTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211691 protein sequence
Red=Cloning site Green=Tags(s) MDVDGRWRNLPSGPSLKHLTDPSYGIPPEQQKAALQDLTRAHVDSFNYAALEGLSHAVQAIPPFEFAFKD ERISLTIVDAVISPPSVPKGTICKDLNVYPAECRGRKSTYRGRLTADISWAVNGVPKGIIKQFLGYVPIM VKSKLCNLYNLPPRVLIEHHEEAEEMGGYFIINGIEKVIRMLIVPRRNFPIAMVRPKWKSRGLGYTQFGV SMRCVREEHSAVNMNLHYVENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEEDSFFRN SVSQMLRIVIEEGCHSQKQVLNYLGECFRVKLSLPDWYPNVEAAEFLLNQCICIHLQSNTDKFYLLCLMT RKLFALARGECMDDNPDSLVNQEVFSPGQLFLMFLKEKMENWLVSIKIVLDKRAQKANVSINNENLMKIF SMGTELTRPFEYLLATGNLRSKTGLGFLQDSGLCVVADKLNFLRYLSHFRCVHRGAAFAKMRTTTVRRLL PESWGFLCPVHTPDGAPCGLLNHLTAVCEVVTKFVYTASIPALLCGLGVTPVDTAPCRPYSDCYPVLLDG VMVGWVDKDLAPEVADTLRRFKVLREKRIPPWMEVALIPMTGKPSLYPGLFLFTTPCRLVRPVQNLELGR EELIGTMEQLFMNVAIFEDEVFGGISTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPL LTYQNRSDNKLYRLQTPQSPLVRPCMYDFYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAH GSVYKSEFIDLSEKFKQGEDNLVFGVKPGDPRVMQKLDDDGLPFIGAKLEYGDPYYSYLNLNTGEGFVVY YKSKENCVVDNIKVCSNDMGSGKFKCICITVRIPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMM PDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLY SGISGMELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPLGGRNVQGGIRFGEMERDALLAHGT SFLLHDRLFNCSDRSVAHMCVECGSLLSPLLEKPPPSWSAMRNRKYNCTVCGRSDTIDTVSVPYVFRYFV AELAAMNIKVKLDVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009086 |
| ORF Size | 3408 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009086.2, NP_033112.2 |
| RefSeq Size | 4017 bp |
| RefSeq ORF | 3408 bp |
| Locus ID | 20017 |
| UniProt ID | P70700 |
| MW | 128.2 kDa |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223668 | Polr1b (untagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b), (10ug) |
CNY 14,540.00 |
|
| MR211691L3 | Lenti ORF clone of Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b) |
CNY 10,260.00 |
|
| MR211691L4 | Lenti ORF clone of Polr1b (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b) |
CNY 10,260.00 |
