Myt1l (NM_001093778) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900046C06Rik; 2900093J19Rik; C630034G21Rik; mKIAA1106; Nztf1; Pmng1; Png-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223855 representing NM_001093778 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGGACTCTGAGGAGAAGCGCCATCGCACACGGTCCAAAGGGGTTCGAGTTCCTGTGGAGCCAG CCATACAAGAGCTGTTCAGCTGTCCCACTCCAGGCTGCGACGGCAGTGGTCACGTCAGTGGCAAATATGC ACGACACAGAAGTGTATATGGTTGTCCCTTGGCTAAAAAAAGAAAAACGCAAGATAAACAGCCCCAAGAA CCTGCTCCCAAGCGAAAACCATTTGCAGTAAAAGCAGATAGTTCCTCAGTAGACGAATGTTATGAGAGTG ATGGTACTGAAGACATGGATGATAAGGAGGAAGATGATGATGAGGAGTTCTCTGAAGACAATGATGAGCA AGGGGATGATGACGACGAAGATGAGGTGGATCGGGAAGACGAGGAGGAGATCGAGGAGGAAGATGATGAA GAAGATGATGATGATGAAGATGGTGACGATGTAGAAGAGGAAGAAGAGGATGATGATGAAGAGGAGGAAG AAGAGGAAGAGGAAGAAGAAAATGACCATCAAATGAGTTGTACTCGAATAATGCAGGACACAGACAAGGA TGATAACAACAATGATGAGTATGATAACTATGATGAACTGGTAGCTAAGTCGCTATTAAATCTTGGCAAA ATTGCTGAGGATGCAGCATACCGAGCCAGGACTGAATCAGAGATGAACAGCAATACCTCCAATAGTCTGG AGGACGATAGTGACAAAAACGAAAACCTCGGTCGGAAAAGCGAACTGAGTCTAGACTTAGACAGTGATGT TGTTAGAGAAACAGTGGACTCCCTTAAGCTGTTAGCACAAGGACATGGTGTTGTGCTATCAGAGAATATC AGTGACAGAAGTTATGCTGAGGGGATGTCACAGCAGGACAGTAGAAATATGAACTATGTCATGCTAGGGA AGCCCATGAACAATGGACTCATGGAGAAGATGGTGGAGGAGAGTGATGAGGAAGTGTGTCTAAGTAGTCT AGAGTGCCTGAGGAACCAGTGCTTTGACCTGGCCAGGAAACTCAGCGAGACCAACCCACAGGACAGGAGT CAGCCACCCAACATGAGTGTGCGCCAACATGTCCGGCAAGAGGACGACTTCCCTGGGAGGACGCCAGACA GGAGCTACTCGGATATGATGAACCTTATGCGGCTGGAGGAGCAGCTCAGTCCCAGGTCTAGAACGTTCTC CAGCTGTGCCAAGGAGGATGGGTGTCATGAGAGGGATGATGACACCACCTCAGTGAACTCAGACAGGTCT GAGGAAGTGTTTGACATGACCAAGGGCAACCTGACTCTGCTAGAGAAAGCCATTGCCTTGGAGACAGAGA GAGCCAAGGCCATGCGGGAGAAGATGGCCATGGATGCTGGGAGAAGGGATAACCTGAGATCCTATGAGGA CCAGTCTCCAAGACAGCTGGCTGGGGAAGACAGAAAATCCAAATCCAGTGACAGCCATGTCAAAAAGCCA TACTATGATCCCTCAAGAACAGAAAAGAGAGAGAGCAAGTGTCCAACCCCCGGGTGTGATGGAACCGGCC ACGTAACTGGGCTTTACCCGCATCACCGCAGTCTGTCTGGATGCCCGCACAAAGATAGGGTCCCTCCAGA AATTCTTGCCATGCATGAAAATGTTCTCAAGTGTCCCACTCCAGGCTGCACAGGGCGAGGGCATGTGAAT AGCAACAGGAACTCGCACAGAAGCCTCTCTGGATGCCCCATTGCTGCTGCAGAAAAACTGGCAAAGGCCC AAGAGAAACACCAGAGCTGTGATGTGTCCAAATCCAACCAGGCCTCAGACCGAGTCCTCAGGCCAATGTG CTTTGTCAAACAGCTTGAGATTCCTCAGTATGGCTACAGAAACAATGTTCCCACAACCACACCACGCTCC AACCTGGCCAAGGAGCTTGAGAAATACTCCAAGACTTCGTTTGAGTACAACAGTTACGACAACCATACTT ATGGCAAAAGAGCCATAGCTCCCAAGGTGCAAACCAGGGACATATCCCCCAAAGGATATGACGATGCCAA GCGGTACTGCAAGAATGCCAGCCCCAGCAGCAGCACCACCAGCAGCTATGCACCTAGCAGCAGCAGCAAC CTCAGCTGTGGTGGTGGCAGCAGCGCCAGTAGCACGTGTAGCAAGAGCAGCTTTGACTACACACATGACA TGGAGGCCGCACACATGGCAGCCACAGCCATTCTCAACCTGTCCACACGTTGTCGTGAAATGCCACAGAA CCTGTCCACCAAGCCACAGGACCTGTGTACTGCCCGGAACCCAGACATGGAGGTGGATGAGAATGGCACC CTGGACCTGAGCATGAACAAGCAGAGGCCTCGAGACAGCTGCTGCCCAGTCCTGACACCCCTGGAACCCA TGTCTCCGCAGCAGCAGGCCGTGATGAGCAGCCGATGCTTCCAGCTGAGCGAGGGGGATTGCTGGGACTT GCCTGTAGACTACACCAAAATGAAGCCTCGGAGGGTAGATGAGGATGAGCCCAAAGAGATTACCCCAGAA GACTTGGACCCATTCCAGGAGGCTCTGGAAGAAAGACGGTATCCAGGGGAGGTGACCATCCCAAGCCCCA AACCCAAGTACCCTCAGTGCAAGGAAAGCAAAAAGGACTTAATAACTCTGTCTGGCTGCCCCCTGGCGGA CAAAAGCATTCGAAGTATGCTGGCCACCAGTTCCCAAGAGCTCAAGTGCCCCACCCCTGGCTGTGACGGT TCTGGACACATCACTGGCAATTACGCTTCTCATCGAAGCCTTTCTGGGTGCCCGAGAGCAAAGAAGAGTG GCATCCGGATAGCACAGAGCAAAGAGGACAAGGAAGACCAGGAGCCAATCAGGTGTCCGGTACCTGGCTG TGACGGTCAGGGACACATCACTGGGAAGTATGCATCCCACCGCAGCGCCTCCGGGTGTCCCTTGGCAGCC AAGAGGCAGAAAGATGGGTACCTTAATGGCTCCCAGTTCTCCTGGAAGTCGGTCAAGACGGAGGGCATGT CCTGCCCTACCCCCGGGTGTGATGGGTCAGGACACGTCAGTGGCAGCTTCCTCACACACCGCAGCTTGTC AGGATGTCCAAGAGCCACATCAGCAATGAAGAAAGCAAAGCTGTCTGGAGAACAGATGTTGACTATCAAG CAGCGAGCCAGCAACGGTATAGAAAATGATGAAGAAATCAAGCAGTTAGATGAAGAGATCAAGGAGCTTA ATGAGTCCAATTCCCAGATGGAGGCTGACATGATCAAACTCAGAACTCAGATCACCACAATGGAGAGCAA CCTGAAGACGATTGAGGAGGAGAACAAAGTCATTGAACAGCAGAATGAGTCGCTCTTGCACGAGTTGGCC AACCTGAGCCAGTCCCTGATCCACAGCCTCGCCAACATCCAGCTGCCTCACATGGATCCAATCAATGAAC AAAATTTTGATGCTTACGTGACTACTTTGACGGAAATGTATACAAATCAAGATCGTTATCAGAGTCCAGA AAATAAAGCCCTACTGGAAAATATAAAGCAGGCTGTGAGAGGAATTCAGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001093778
Insert Size	3555 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001093778.1, NP_001087247.1
RefSeq Size	7189 bp
RefSeq ORF	3555 bp
Locus ID	17933
Gene Summary	Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation (PubMed:28379941). In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs (PubMed:28379941). Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1 (PubMed:28379941). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B (PubMed:28379941). The 5'-AAGTT-3' core motif is absent from the promoter of neural genes (PubMed:28379941).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses alternate in-frame splice sites in the 5' and mid-coding regions, compared to variant 1, resulting in a shorter protein (isoform 3).

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