Polg (NM_017462) Mouse Untagged Clone
CAT#: MC223968
Polg (untagged) - Mouse polymerase (DNA directed), gamma (Polg), (10ug)
CNY 12,720.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AA409516; PolgA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC223968 representing NM_017462
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCCTGCTCTGGAAGAAGGTGGCTGGCGCCAAGGTCGCCTCAGGGCCAGTACCAGCAACCGCGC GCTGGGTCTCCAGCTCCGTCCTCGACCCCGTCCCCAGCGACGGGCGGCCGCCGTCGCAAATGCCCTCCTC CGAGAATGGGCAGCTGCGGCTCAACCCTCTGCTCATCCAGATGTTGTCGAGAGGCCTGCACGAGCAGATC TTCGGGTGCGGCGGGGAAATGCCCGACGAGGCCGCGGTGCAGCGCAGCGTAGAGCACCTGCAGAAGCACG GGCTCTGGGGGCAGCCGGCCACGCCCTTGCCAGACGTGGAGCTGCGCCTGCCCCGGCTCTTCGGGGGCAA CCTGGACCAGCACTTCCGCCTCCTGGCCCAGAAGCAGAGCCTGCCTTACTTGGAGGCGGCCGCCTCGTTA TTGGAGGCCCAATTGCCCCCCGAGCCCAAGAGCTGGGCCTGGGCGGAGGGCTGGACCCGGTACGGCCCCG AGGGGGAGGCCGAACCCGTGGCCATCCCCGAGGAGCGGGCCCTGGTGTTCGACGTGGAGGTCTGCTTGGC AGAGGGAACCTGCCCCACTTTGGCGGTGGCCATATCCCCCTCGGCCTGGTATTCCTGGTGCAGCCGGCGG CTGGTGGAAGAGCGTTACTCTTGGACCAGCCAGCTATCGCCGGCTGACCTAATCCCTTTGGGGGGCTCCA CTAGTGCCAGCAGCTCCACCAAGCAGGATGGGCAGGAACAGTTAGTGGTGGGGCACAATGTTTCCTTTGA CCGAGCCCATATCAGGGAACAGTATCTGATTCAGGACTCCCGGATGCGTTTTCTCGATACTATGAGCATG CACATGGCCATCTCGGGGCTGAGCAGCTTCCAGCGCAGCCTGTGGATGGGAGCCAAGCAGGGAAAGCACA AGACCCAGCAGTCCACAAAGCGAGGGCAGAAGTCCCCGAGGAAAGCCAATGGTCCAGCGATCTCATCTTG GGACTGGATGGATATCAGCAGTGCCAATAATCTTGCAGATGTGCACAACCTTTATGTGGGGGGACCTCCC TTAGAGAAGGAGCCTCGGGAGCTGTTCGTCAAAGGCAGCATGAGGGATATCCGAGAGAACTTCCAGGATC TGATGCAGTACTGCGCCCGTGATGTGTGGGCCACCTTTGAGGTTTTCCAGCAGCAGCTGCCACTCTTCTT GGAGAGGTGTCCCCACCCAGTGACTCTGGCTGGCATGCTGGAGATGGGTGTGTCCTACCTGCCTGTCAAC CAGAACTGGGAGCGTTACCTGACAGAGGCACAGAACACATATGAGGAGCTCCAGCGGGAGATGAAGAAGT CGCTGATGGATCTGGCTAATGATGCCTGTCAGTTGCTCTCAGGAGAGAGGTACAAAGAAGACCCTTGGCT CTGGGACCTAGAATGGGATTTGCAGGAGTTTAAGCAGAAAAAGGCAAAGAAGGTGAAGAAGCCAGCCTCA GCCAGCAAGTTGCCCATCGAGGGAGCTGGGCCCTTTGGGGATCCCATGGATCAGGAAGATCCTGGCCCGC CCAGCGAGGAGGAGGAGCTTCAGCGAAGTGTAACAGCCCACAACCGGTTACAGCAGCTGAGGAGCACCAC GGACCTCCTGCCTAAGCGACCCCAGCACCTTCCAGGACACCCTGGGTGGTACCGGAAGCTCTGCCCTCGA CTAGATGACCCTGCCTGGGCTCCCGGCCCCAGCCTCCTCAGCCTGCAAATGCGGGTCACTCCTAAGCTCA TGGCACTGACCTGGGATGGTTTTCCTCTACACTACTCAGACTCTCATGGTTGGGGCTACCTAGTGCCTGG GCGTCGGGATAATCTGACCGAGCCGCCAGTGAGCCCCACTGTAGAATCCGCTGCAGTAACCTGCCCCTAC AGAGCCATCGAGTCCTTATACAGGAAGCACTGCCTTGAACAGGGGAAGCAGCAATTGGAGCCCCAGGAGG TAGACCTGGCTGAGGAGTTCTTACTCACTGACAGTAGTGCCATGTGGCAAACGGTAGAAGAACTGGGCTG CTTAGACGTAGAGGCTGAGGCCAAGATGGAGAATTCAGGGCTGAGCCAACCTCTAGTTCTGCCTGCTGCT TGTGCCCCCAAATCCAGCCAGCCCACGTATCACCATGGCAACGGACCTTATAATGATGTGAATATCCCTG GTTGCTGGTTTTTCAAGCTGCCTCACAAGGACGGTAACAACTACAATGTGGGCAGTCCCTTTGCCAAAGA TTTCCTGCCCAAGATGGAGGATGGCACCCTGCAGGCTGGCCCAGGAGGTGCCAGTGGGCCTCGTGCCCTG GAAATAAATAAAATGATTTCTTTTTGGAGGAATGCTCATAAACGTATCAGTTCCCAGATGGTGGTATGGC TCCCCAGGTCAGCTCTGCCCCGGGTTGTGACCAGGCACCCCAGCTTCGATGAGGAAGGCCACTATGGGGC CATCCTACCCCAGGTGGTGACTGCTGGCACCATCACCCGTCGAGCTGTGGAGCCCACGTGGCTCACTGCC AGTAATGCTCGGCCTGACCGGGTAGGCAGTGAGTTGAAAGCCATGGTGCAGGCTCCACCTGGCTATGTCC TTGTGGGTGCTGATGTGGACTCACAGGAACTGTGGATCGCAGCTGTACTTGGAGATGCTCACTTTGCTGG GATGCATGGCTGCACGGCCTTTGGCTGGATGACTCTGCAGGGCAGGAAGAGCAGAGGCACTGATCTGCAC AGCAAGACAGCTGCCACTGTGGGCATCAGCCGAGAGCATGCCAAAATCTTCAACTATGGCCGCATCTATG GGGCTGGGCAGTCCTTTGCTGAGCGCCTACTGATGCAGTTCAACCACAGGCTCACAAGGCAGGAGGCAGC TGAGAAGGCCCAGCAGATGTATGCAGTCACAAAAGGCCTGCGCCGGTATCGGCTGTCTGCGGATGGTGAG TGGCTGGTGAAACAGTTGAATCTTCCTGTGGACAGGACAGAGGACGGCTGGGTCTCCCTACAAGATCTTC GAATGATCCGAAGAGAAGCTTCAAGGAAGTCACGATGGAAGAAGTGGGAGGTAGCCTCTGAACGAGCATG GACAGGGGGCACAGAGTCAGAAATGTTTAATAAGCTGGAGAGTATTGCCATGTCTGATACACCACGTACC CCAGTACTGGGCTGCTGCATCAGCAGAGCCTTGGAGCCCTCAGTTGTCCAGGGAGAGTTTATAACCAGTC GTGTGAACTGGGTGGTACAGAGCTCTGCTGTAGACTACTTACATCTCATGCTTGTGGCCATGAAGTGGCT GTTTGAGGAATTTGCCATTGATGGGCGCTTCTGCATCAGCATCCACGACGAGGTTCGCTACCTGGTGCGT GAGGAGGACCGCTACCGTGCCGCCCTGGCACTGCAGATCACCAATCTCCTGACCAGGTGCATGTTTGCCT ATAAGCTGGGTCTGAATGATCTGCCCCAGTCAGTCGCCTTTTTCAGTGCAGTAGACATTGACCAGTGCCT CAGGAAGGAAGTGACCATGGACTGTAAAACTCCTTCTAACCCAACTGGGATGGAAAGGAGATATGGGATC CCCCAGGGTGAAGCACTGGATATTTACCAGATAATTGAACTCACCAAAGGCTCCTTGGAAAAACGAAGCC AGCCTGGACCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_017462 |
Insert Size | 3654 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_017462.2, NP_059490.2 |
RefSeq Size | 4596 bp |
RefSeq ORF | 3654 bp |
Locus ID | 18975 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226589 | Polg (tGFP-tagged) - Mouse polymerase (DNA directed) gamma (Polg), (10ug) |
CNY 13,400.00 |
|
MR226589 | Polg (Myc-DDK-tagged) - Mouse polymerase (DNA directed), gamma (Polg) |
CNY 12,712.00 |
|
MR226589L3 | Lenti ORF clone of Polg (Myc-DDK-tagged) - Mouse polymerase (DNA directed), gamma (Polg) |
CNY 14,060.00 |
|
MR226589L4 | Lenti ORF clone of Polg (mGFP-tagged) - Mouse polymerase (DNA directed), gamma (Polg) |
CNY 15,112.00 |