Abcb1a (NM_011076) Mouse Untagged Clone

CAT#: MC224126

Abcb1a (untagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a), (10ug)



  "NM_011076" in other vectors (4)

CNY 13,336.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Abcb4; Evi32; mdr-3; Mdr1a; Mdr3; P-gp; Pgp; Pgy-3; Pgy3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224126 representing NM_011076
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACTTGAAGAGGACCTTAAGGGAAGAGCAGACAAGAACTTCTCAAAGATGGGCAAAAAGAGTAAAA
AGGAGAAGAAAGAAAAGAAACCAGCAGTCAGTGTGCTTACAATGTTTCGTTATGCAGGTTGGCTGGACAG
GTTGTACATGCTGGTGGGAACTCTGGCTGCTATTATCCATGGAGTGGCGCTCCCACTTATGATGCTGATC
TTTGGTGACATGACAGATAGCTTTGCAAGTGTAGGAAACGTCTCTAAAAACAGTACTAATATGAGTGAGG
CCGATAAAAGAGCCATGTTTGCCAAACTGGAGGAAGAAATGACCACGTACGCCTACTATTACACCGGGAT
TGGTGCTGGTGTGCTCATAGTTGCCTACATCCAGGTTTCATTTTGGTGCCTGGCAGCTGGAAGACAGATA
CACAAGATCAGGCAGAAGTTTTTTCATGCTATAATGAATCAGGAGATAGGCTGGTTTGATGTGCATGACG
TTGGGGAGCTCAACACCCGGCTCACAGATGATGTTTCCAAAATTAATGAAGGAATTGGTGACAAAATCGG
AATGTTCTTCCAGGCAATGGCAACATTTTTTGGTGGTTTTATAATAGGATTTACCCGTGGCTGGAAGCTA
ACCCTTGTGATTTTGGCCATCAGCCCTGTTCTTGGACTGTCAGCTGGTATTTGGGCAAAGATATTGTCTT
CATTTACTGATAAGGAACTCCATGCTTATGCAAAAGCTGGAGCAGTTGCTGAAGAAGTCTTAGCAGCCAT
CAGAACTGTGATTGCGTTTGGAGGACAAAAGAAGGAACTTGAAAGGTACAATAACAACTTGGAAGAAGCT
AAAAGGCTGGGGATAAAGAAAGCTATCACGGCCAACATCTCCATGGGTGCAGCTTTTCTCCTTATCTATG
CATCATATGCTCTGGCATTCTGGTATGGGACTTCCTTGGTCATCTCCAAAGAATACTCTATTGGACAAGT
GCTCACTGTCTTCTTTTCCGTGTTAATTGGAGCATTCAGTGTTGGACAGGCATCTCCAAATATTGAAGCC
TTCGCCAATGCACGAGGAGCAGCTTATGAAGTCTTCAAAATAATTGATAATAAGCCCAGTATAGACAGCT
TCTCAAAGAGTGGGCACAAACCAGACAACATACAAGGAAATCTGGAATTTAAGAATATTCACTTCAGTTA
CCCATCTCGAAAAGAAGTTCAGATCTTGAAGGGCCTCAATCTGAAGGTGAAGAGCGGACAGACGGTGGCC
CTGGTTGGCAACAGTGGCTGTGGAAAAAGCACAACTGTCCAGCTGATGCAAAGGCTCTACGACCCCCTAG
ATGGCATGGTCAGTATCGACGGACAGGACATCAGAACCATCAATGTGAGGTATCTGAGGGAGATCATTGG
TGTGGTGAGTCAGGAACCTGTGCTGTTTGCCACCACGATCGCCGAGAACATTCGCTATGGCCGAGAAGAT
GTCACCATGGATGAGATTGAGAAAGCTGTCAAGGAAGCCAATGCCTATGACTTCATCATGAAACTGCCCC
ACCAATTTGACACCCTGGTTGGTGAGAGAGGGGCGCAGCTGAGTGGGGGACAGAAACAGAGAATCGCCAT
TGCCCGGGCCCTGGTCCGCAATCCCAAGATCCTTTTGTTGGACGAGGCCACCTCAGCCCTGGATACAGAA
AGTGAAGCTGTGGTTCAGGCCGCACTGGATAAGGCTAGAGAAGGCCGGACCACCATTGTGATAGCTCATC
GCTTGTCTACCGTTCGTAATGCTGACGTCATTGCTGGTTTTGATGGTGGTGTCATTGTGGAGCAAGGAAA
TCATGATGAGCTCATGAGAGAAAAGGGCATTTACTTCAAACTTGTCATGACACAGACAGCAGGAAATGAA
ATTGAATTAGGAAATGAAGCTTGTAAATCTAAGGATGAAATTGATAATTTAGACATGTCTTCAAAAGATT
CAGGATCCAGTCTAATAAGAAGAAGATCAACTCGCAAAAGCATCTGTGGACCACATGACCAAGACAGGAA
GCTTAGTACCAAAGAGGCCCTGGATGAAGATGTACCTCCAGCTTCCTTTTGGCGGATCCTGAAGTTGAAT
TCAACTGAATGGCCTTATTTTGTGGTTGGTATATTCTGTGCCATAATAAATGGAGGCTTACAGCCAGCAT
TCTCCGTAATATTTTCAAAAGTTGTAGGGGTTTTTACAAATGGTGGCCCCCCTGAAACCCAGCGGCAGAA
CAGCAACTTGTTTTCCTTGTTGTTTCTGATCCTTGGGATCATTTCTTTCATTACATTTTTTCTTCAGGGC
TTCACATTTGGCAAAGCTGGAGAGATCCTCACCAAGCGACTCCGATACATGGTTTTCAAATCCATGCTGA
GACAGGATGTGAGCTGGTTTGATGACCCTAAAAACACCACCGGAGCACTGACCACCAGGCTCGCCAACGA
TGCTGCTCAAGTGAAAGGGGCTACAGGGTCTAGGCTTGCTGTGATTTTCCAGAACATAGCAAATCTTGGG
ACAGGAATCATCATATCCCTAATCTATGGCTGGCAACTAACACTTTTACTCTTAGCAATTGTACCCATCA
TTGCGATAGCAGGAGTGGTTGAAATGAAAATGTTGTCTGGACAAGCACTGAAAGATAAGAAGGAACTAGA
AGGTTCTGGAAAGATTGCTACGGAAGCAATTGAAAACTTCCGCACTGTTGTCTCTTTGACTCGGGAGCAG
AAGTTTGAAACCATGTATGCCCAGAGCTTGCAGATACCATACAGAAATGCGATGAAGAAAGCACACGTGT
TTGGGATCACGTTCTCCTTCACCCAGGCCATGATGTATTTTTCTTATGCTGCTTGTTTCCGGTTCGGTGC
CTACTTGGTGACACAACAACTCATGACTTTTGAAAATGTTCTGTTAGTATTCTCAGCTATTGTCTTTGGT
GCCATGGCAGTGGGGCAGGTCAGTTCATTCGCTCCTGACTATGCGAAAGCCACAGTGTCAGCATCCCACA
TCATCAGGATCATTGAGAAAACCCCCGAGATTGACAGCTACAGCACGCAAGGCCTAAAGCCGAATATGTT
GGAAGGAAATGTGCAATTTAGTGGAGTCGTGTTCAACTATCCCACCCGACCCAGCATCCCAGTGCTTCAG
GGGCTGAGCCTTGAGGTGAAGAAGGGCCAGACGCTGGCCCTGGTGGGCAGCAGTGGCTGCGGGAAGAGCA
CAGTGGTCCAGCTGCTCGAGCGCTTCTACGACCCCATGGCTGGATCAGTGTTTCTAGATGGCAAAGAAAT
AAAGCAACTGAATGTCCAGTGGCTCCGAGCACAGCTGGGCATTGTGTCCCAAGAGCCCATTCTCTTTGAC
TGCAGCATCGCAGAGAACATTGCCTACGGAGACAACAGCCGGGTCGTGTCTTATGAGGAGATTGTGAGGG
CAGCCAAGGAGGCCAACATCCACCAGTTCATCGACTCGCTACCTGATAAATACAACACCAGAGTAGGAGA
CAAAGGCACTCAGCTGTCGGGTGGGCAGAAGCAGCGCATCGCCATCGCACGCGCCCTCGTCAGACAGCCT
CACATTTTACTTCTGGACGAAGCAACATCAGCTCTGGATACAGAAAGTGAAAAGGTTGTCCAGGAAGCGC
TGGACAAAGCCAGGGAAGGCCGCACCTGCATTGTGATCGCTCACCGCCTGTCCACCATCCAGAACGCGGA
CTTGATCGTGGTGATTCAGAACGGCAAGGTCAAGGAGCACGGCACCCACCAGCAGCTGCTGGCGCAGAAG
GGCATCTACTTCTCAATGGTCAGTGTGCAGGCTGGAGCAAAGCGCTCATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_011076
Insert Size 3831 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011076.2, NP_035206.2
RefSeq Size 4977 bp
RefSeq ORF 3831 bp
Locus ID 18671
UniProt ID P21447
Gene Summary The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a p-glycoprotein which actively transports a variety of hydrophobic amphipathic drugs and plays a major role in the blood-brain barrier permeability of certain drugs. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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