Abcb1a (NM_011076) Mouse Tagged ORF Clone
CAT#: MG227524
- TrueORF®
Abcb1a (tGFP-tagged) - Mouse ATP-binding cassette sub-family B (MDR/TAP) member 1A (Abcb1a), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_011076" in other vectors (4)
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CNY 13,970.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Abcb4; Evi32; mdr-3; Mdr1a; Mdr3; P-gp; Pgp; Pgy-3; Pgy3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227524 representing NM_011076, codon optimized.
Due to the complexity of NM_011076, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGGAGGAGGACCTCAAGGGCCGAGCGGACAAAAATTTTAGTAAGATGGGCAAGAAATCCAAGA AAGAGAAGAAGGAAAAAAAGCCAGCCGTGTCTGTGCTCACAATGTTCCGATACGCAGGGTGGCTTGATCG GCTCTACATGCTCGTCGGAACGTTGGCTGCCATCATCCACGGAGTAGCGCTGCCTTTGATGATGTTGATT TTCGGTGACATGACCGACTCATTCGCATCCGTTGGGAACGTGTCTAAGAACAGCACAAACATGAGTGAGG CTGACAAAAGGGCAATGTTCGCAAAGCTTGAAGAGGAGATGACGACCTACGCATACTACTACACAGGTAT CGGCGCAGGGGTCCTCATCGTGGCCTACATCCAGGTGAGTTTCTGGTGTCTGGCCGCTGGCAGACAAATT CACAAGATCAGACAAAAATTCTTTCACGCTATTATGAATCAGGAGATTGGATGGTTTGATGTCCACGATG TCGGAGAGCTGAACACCCGGCTGACCGACGATGTTAGCAAGATTAATGAAGGTATCGGAGACAAGATCGG CATGTTTTTCCAGGCTATGGCGACCTTCTTCGGCGGTTTTATTATAGGATTCACCCGGGGTTGGAAACTC ACTCTGGTGATACTTGCTATCAGTCCAGTGCTCGGACTGTCTGCTGGCATATGGGCCAAAATTCTCAGCT CATTCACTGACAAAGAACTGCACGCATATGCCAAAGCAGGTGCAGTGGCAGAGGAAGTACTCGCTGCCAT TCGCACCGTCATAGCCTTTGGGGGTCAGAAGAAGGAGCTGGAAAGGTACAACAATAACCTGGAAGAGGCT AAGAGGCTCGGAATAAAAAAGGCCATTACCGCCAACATCAGCATGGGAGCGGCATTCCTTCTCATCTACG CCTCTTACGCCCTGGCCTTCTGGTACGGAACTAGCCTGGTCATTTCCAAAGAATACAGCATCGGGCAGGT GCTTACCGTGTTCTTCTCCGTCCTCATCGGCGCTTTCTCCGTCGGTCAGGCCAGCCCCAATATCGAGGCA TTCGCTAATGCCAGAGGAGCCGCATATGAAGTATTTAAGATCATTGACAACAAGCCATCCATCGACTCTT TTTCTAAATCTGGACACAAGCCAGATAACATCCAAGGGAACCTGGAATTTAAAAATATTCACTTCAGTTA TCCCTCCCGGAAGGAGGTCCAGATCCTTAAAGGACTCAATCTTAAAGTCAAATCCGGCCAGACTGTAGCA CTCGTCGGTAATTCTGGGTGTGGGAAGAGTACCACAGTGCAGTTGATGCAGCGCCTCTATGACCCCTTGG ACGGGATGGTCAGCATAGACGGGCAAGATATTAGGACAATCAACGTTCGATATCTGCGAGAAATTATCGG TGTCGTGAGCCAAGAACCAGTCCTCTTCGCCACTACTATTGCAGAGAACATCAGATATGGCCGAGAGGAT GTCACAATGGACGAGATTGAGAAGGCTGTCAAAGAGGCTAATGCTTACGATTTCATCATGAAGCTGCCTC ACCAGTTTGACACACTCGTTGGTGAGAGGGGCGCTCAATTGTCCGGCGGACAGAAGCAAAGGATCGCAAT TGCGCGGGCGCTCGTGCGCAACCCAAAAATATTGCTGTTGGACGAAGCCACCTCTGCACTGGACACCGAG TCCGAGGCCGTGGTGCAGGCTGCCCTTGATAAGGCTCGGGAGGGCCGAACCACTATTGTCATTGCACACC GCCTCTCCACAGTGAGAAACGCCGACGTCATCGCAGGGTTCGATGGCGGCGTCATTGTGGAACAGGGCAA CCACGATGAACTCATGCGCGAGAAGGGAATATATTTTAAACTGGTGATGACCCAGACCGCGGGCAATGAG ATCGAACTGGGAAACGAGGCCTGTAAGAGCAAGGATGAAATTGACAACCTGGACATGTCTAGCAAAGACT CCGGATCTTCTTTGATTAGGAGGCGCTCCACGCGGAAGTCCATTTGTGGACCACATGATCAAGACCGCAA GCTCTCCACCAAGGAGGCACTGGACGAGGACGTTCCTCCTGCCTCATTCTGGCGGATTCTGAAATTGAAC AGCACAGAATGGCCATACTTTGTTGTGGGAATCTTTTGCGCTATTATTAACGGGGGGCTCCAACCAGCAT TCTCCGTCATTTTCTCCAAGGTGGTGGGCGTCTTCACTAATGGTGGACCACCAGAAACTCAAAGACAGAA TAGTAACTTGTTTTCACTTCTCTTCCTTATACTCGGCATTATTAGCTTTATTACGTTTTTCCTGCAGGGG TTTACATTTGGCAAGGCTGGAGAGATTCTGACAAAGCGCCTGAGATACATGGTCTTTAAGTCAATGCTTA GGCAGGACGTCAGCTGGTTCGACGATCCCAAAAATACTACCGGAGCTCTTACCACTCGCCTTGCCAATGA CGCCGCTCAGGTGAAGGGAGCTACAGGCAGTCGACTTGCTGTAATCTTCCAGAACATCGCTAACTTGGGG ACTGGAATCATCATATCCTTGATATATGGCTGGCAACTTACATTGCTTCTTCTCGCCATTGTTCCTATTA TTGCAATCGCTGGAGTCGTTGAGATGAAAATGCTGTCAGGGCAGGCCCTGAAAGACAAAAAGGAATTGGA GGGTAGTGGCAAAATTGCCACAGAAGCAATTGAAAACTTCAGAACTGTTGTCTCTTTGACGAGAGAACAA AAATTCGAAACAATGTATGCCCAGTCTTTGCAGATCCCATACAGGAACGCAATGAAAAAGGCCCACGTGT TCGGTATCACTTTTTCTTTTACACAGGCAATGATGTATTTCTCTTACGCTGCCTGTTTTAGATTCGGCGC GTATCTTGTGACGCAGCAGCTGATGACTTTCGAGAATGTCCTTCTTGTATTTAGTGCCATCGTGTTTGGG GCAATGGCGGTCGGGCAGGTAAGCTCCTTCGCCCCTGACTATGCTAAGGCAACGGTGAGCGCTAGCCATA TCATCAGGATCATAGAGAAGACTCCCGAGATAGATTCCTATTCTACCCAGGGGCTGAAGCCTAACATGCT GGAAGGCAACGTCCAATTTTCTGGGGTTGTGTTCAATTATCCGACTCGACCCTCCATTCCCGTACTGCAA GGCCTCTCTCTCGAGGTCAAAAAGGGCCAAACTCTCGCCTTGGTTGGCTCATCTGGATGTGGCAAGAGCA CTGTTGTGCAATTGCTGGAGCGATTTTATGATCCAATGGCTGGCTCAGTGTTTCTGGACGGCAAGGAGAT CAAGCAGCTCAACGTGCAGTGGCTGCGCGCCCAGCTTGGGATAGTCAGTCAGGAACCAATCCTGTTTGAC TGCTCAATAGCTGAGAACATTGCCTACGGGGACAATAGCCGGGTCGTTTCATACGAGGAAATTGTTAGGG CTGCGAAAGAGGCTAACATTCATCAATTTATCGACTCCCTGCCTGACAAGTATAATACAAGGGTTGGAGA TAAAGGTACACAGCTGTCAGGTGGTCAGAAGCAGCGGATCGCTATTGCTCGCGCTCTGGTTCGACAGCCA CACATCTTGCTTTTGGATGAAGCCACCTCAGCATTGGATACCGAGTCCGAGAAAGTCGTGCAGGAGGCTC TGGATAAGGCTAGAGAAGGAAGGACATGTATCGTAATCGCACATCGGCTTTCCACTATCCAGAACGCAGA CCTGATAGTGGTCATCCAGAACGGAAAAGTCAAGGAACATGGGACCCACCAGCAGCTTCTTGCTCAGAAG GGAATTTACTTCTCCATGGTGTCCGTCCAGGCAGGCGCTAAGCGGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227524 representing NM_011076
Red=Cloning site Green=Tags(s) MELEEDLKGRADKNFSKMGKKSKKEKKEKKPAVSVLTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLI FGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQI HKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKL TLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEA KRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEA FANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVA LVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRED VTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTE SEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTAGNE IELGNEACKSKDEIDNLDMSSKDSGSSLIRRRSTRKSICGPHDQDRKLSTKEALDEDVPPASFWRILKLN STEWPYFVVGIFCAIINGGLQPAFSVIFSKVVGVFTNGGPPETQRQNSNLFSLLFLILGIISFITFFLQG FTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLG TGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQ KFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFG AMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQ GLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFD CSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQP HILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQK GIYFSMVSVQAGAKRS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011076 |
ORF Size | 3828 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011076.2, NP_035206.2 |
RefSeq Size | 5177 bp |
RefSeq ORF | 3831 bp |
Locus ID | 18671 |
UniProt ID | P21447 |
Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a p-glycoprotein which actively transports a variety of hydrophobic amphipathic drugs and plays a major role in the blood-brain barrier permeability of certain drugs. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224126 | Abcb1a (untagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a), (10ug) |
CNY 13,336.00 |
|
MR227524 | Abcb1a (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a). Note: ORF is codon optimized |
CNY 13,328.00 |
|
MR227524L3 | Lenti ORF clone of Abcb1a (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a). Note: ORF is codon optimized |
CNY 14,630.00 |
|
MR227524L4 | Lenti ORF clone of Abcb1a (mGFP-tagged) - Mouse ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a). Note: ORF is codon optimized |
CNY 14,630.00 |