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Mcm9 (NM_027830) Mouse Untagged Clone
CAT＃: MC224169
Mcm9 (untagged) - Mouse minichromosome maintenance complex component 9 (Mcm9), (10ug)
CNY 16,630.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9030408O17Rik; Gm235; Mcmdc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224169 representing NM_027830
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGAGAACTACACGAAATGGAAAATATTGTGACGTGGAACCGGTGTCCCGCTCAAACCCCGCCC CATGCCTCCGAGACCCGCCCCTCAGACGTCTTGTCCGGCCCAAACCCCGCCTCCAGCTCCCCGAGTCCCG CCTCTCTCCCTGCTCCCGCCTCCCGCTCGCAGACTCCAGCGTCCGTCCTGGCGCGCGCCCGCCTGCGTCC GCACCCGGAAGAAGCCCCAGCGGCCGGAAAGTTGAGGCAGTGCGCGGCTCGGGGTCCGCGGGAAGCTCAA GTCCTTCAGAGGCCGAGCGAGAGCAGCGCGAAGAAGCCTGCGCGCCCCCGCGCAAGGCGGCCCCCTCGAG CGGCCGCGCGCACGCCCCGCCCCCTCCAACGCCGCGCGGGTCGGGCTGGGGCGACCACGGCCGCAGCGCG GTCCCGGCGACCAAGACAGTGCGTGTTGAGCCCTACCCACCCTTCAAGATGAATAGTGAGCAGGTCACCC TGGTGGGTCAGGTGTTTGAGTCCTATGTTTCAGAGTACCATAAGAACGATATTCTTCTGATCCTGAAAGA AAGAGATGAAGATGCTCACTACCCGGTTGTGGTTAATGCTATGAGCCTTTTCGAGACCAACATGGAAATT GGGGACTATTTCACCGTGTTCCCCAATGAAGTACTAACAGTTTTTGACAGTGCACTTCGAAGGTCAGCCT TGGCAATTCTGCAGTCCCTTCCTGAGACGGAGGGGTTATCCATGAAGCAGAATCTTCATGCCAGGATATC AGGTTTGCCTGTTTGTCCAGAACTGGTCAGGGAACACATTCCCAAAACCAAGGATGTGGGACACTTCTTA TCTGTCACTGGGACAGTGATCCGAACGAGTCTGGTGAAGGTCTTGGAGTTCGAGCGGGATTACATGTGTA ACAAATGCAAGCATGTGTTCATGGTGGAGGCAGACTTCGAGCAGTATTACACCTTCAGTCGGCCATCGTC ATGTCCAAGTTTAGCCAGCTGTGACTCCTCAAAATTCTCTTGCCTCTCAGACTTGTCTTCATCTCCAGCC AGATGTCGGGATTACCAGGAAATCAAAATTCAGGAGCAGGTGCAAAGGCTGTCTGTTGGAAGTATCCCAC GGTCTATGAAAGTTATTCTGGAAGATGACCTAGTTGACAGTTGCAAATCTGGAGATGACCTCACCATCTA TGGGGTTGTAATGCAACGGTGGAAACCCTTTCAGCGAGATGTACGCTGTGAAGTTGAGATTGTCTTGAAA GCCAACTATGTCCAAGTGAATAATGAGCAATCCTCGGGGATGGTCATGGATGAGGACACTCGAAAAGAAT TTGAAGACTTCTGGGAACACTATAAGAGTGACCCCTTTGCAGGGAGGAATGAAATATTGGCCAGCTTGTG TCCTCAAGTTTTTGGGATGTATCTAGTGAAGCTTGCTGTGGCCATGGTACTGGCTGGTGGAATTCAAAGA ACTGATGCTGCAGGAACCAGGGTTAGAGGGGAATCTCACCTTTTATTGGTTGGGGATCCTGGCACAGGGA AATCACAATTCCTTAAATATGCAGCAAAGATTACCCCAAGGTCCGTTTTGACCACAGGAATTGGATCTAC TAGTGCAGGTCTGACCGTGACAGCTGTCAAAGACTCAGGAGAATGGAATCTAGAGGCTGGGGCTTTGGTG CTTGCAGATGCTGGTCTCTGCTGTATTGACGAATTTAACAGCCTCAAAGAACATGACAGGACAAGCATCC ATGAAGCAATGGAGCAACAAACCATAAGTGTTGCTAAGGCTGGCCTTGTTTGTAAGCTGAACACAAGGAC CACCATCCTGGCAGCAACTAACCCCAAAGGCCAGTATGACCCCAAGGAGTCTGTGTCTGTGAACATTGCC CTCGGGAGCCCACTCTTAAGTCGATTTGACCTTGTCCTGGTTTTGCTCGACACTAGGAATGAAGACTGGG ATCGTATCATTTCTTCCTTTATCTTAGAAAATAAAGGTTATCCAAGCAAATCAGAGAATCTGTGGAGCAT GGAGAAGATGAAAACCTACTTCTGCCTCATTCGGAATCTCCACCCCACACTGTCTGAAGTGAGCAATCAA GTCCTCCTTCGATACTACCAAATGCAAAGGCAGAGTGATTCCCGGAATGCAGCCCGGACAACCATCCGCC TGTTAGAAAGCTTGATCCGATTAGCAGAAGCTCACGCTCGCCTGATGTTCCGCAGTGCCGTGACTCTGGA AGATGCCGTTACAGCTGTATCTGTGATGGAGTCTTCAATGCAGGGAGGTGCTCTGCTAGGAGGTGTGAAT GCTCTCCACACTTCCTTCCCTGAAAACCCCCGTGCACAGTACCAGAGGCAGTGTGAACTCATTCTGGAAA AGCTGGAACTCCAGGGCCTCTTGCAGGAAGAACTTCGAAGACTGGAAAGGTTACAGAATGAGAGTGTACA CCAATGCCAGTCACATTCACTAGAGGAGGAGGTGGCTCCAGGTTCCTGCAGAAATGATCCCAGGGACAAG CCAAGGCTCAGGACTTCAACACAGCAGGAACAGAGCTGTAGCTGGAGTTCCACAGAGAGATCTGGTGCAG ACTCCCCGCCTGGTCCAGGGCTTAATAGACCAACAAGTTGTAACAACTCAGCTGAGAACAGAGATGGCAG AGGTGACGGTTTAGACTGGTTGGACCCCACATCAAGTCCTGAGATTGCACCAGAAAGCACTATTGTGTCT CCCAATGTGAAAACAACTGAGAAAAATGTGAATTTGAAAATCTCCAACAATAAATCTCAGGGCAAGGAGA AGCATGGGCCACAGCAAAGAAGCAAATTATTAGAAGCTGGACATCTTCCATCATCAGGAGCCATGAATGC CCCCTTACGGTCTCACGGTGTTAAGCGTACAAAGGCAAGTCAGGCAGTGGTTGTATCTGAAGCAGGACGG GGTGATGAAGAAGACTCTGTGCCCCGAAGACTCCCCAAGCTGCTGAAGGAGGGGTCACAGAATGTGTGCA GAAGCACAACCAGAGTGCGACCACTGCCACCCACTGTCCCTCTGTCCCTGTCTATCCCTTCACCTGGGTC AGGAAAAAGATCAGGAACACCCAAAAGAAAGAGACGGAAATCTGCTCAGGTGGAAGAGCCTGAACCTGAA GGTATGGAGACTCCAACAGTAAAGCTGGCCAAATTCACATTTAAACAGAAGACAAAACTGACCCACTCCC CCGAAGGCCAAGGCCCCATACCTCCCAGTGCTAGTGAAATAGCAGTTGACAGTTCTAAAATTCCCCAACA AAGAACAAGAAGAGAAGCAGCTGTGCCTGTGGTAGCGCCAGGAAAGTCGACATCCACCTCAGGAGACAGA TGCTCTGACCAGCTGCACGGAAAGACAAAGGAGCTCTCAAGGCAGCCACCAGACAGTAATCCACCAAGAG AGGAGAGGGAACAGGGCCCCAAAAGAAGGGTTATTCAGCCCAAACCGGAGCTTGGGAACCAGGCTGGGCA TTCGCATCTAGCCTGTGAGAAAGACAGAAAGGAAGGGGTTTCCTGCGGTAATAAAAGCAGCAAGGTTCAT GCTGGCACCATAGCCAGACTGGCAAGCTTCTCCTTCACTTCCCCATCAGAATCCAAATCGGAATCCCTCC CTCCTGAAAGGAAGGACAGCAGGGACAGCAGGGACAGCAGGGACAGCAGGGACAGGTGCCACAGCCCTCC TGCGACCACAGCTCCAGTGCTCGGCCAGCAAAGGCAGACTTTCCAGCTGCAGCAGCCCACAGAGAGAGCG AATCTTTCCACCCTCTCCCTCTTCACTCTGTCGGAACTAGATGACGAAGCATTAGATTTTGACTGGGAGG AAGAGATGAGGAAAAAGCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027830 |
| Insert Size | 3873 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027830.2, NP_082106.2 |
| RefSeq Size | 3873 bp |
| RefSeq ORF | 3873 bp |
| Locus ID | 71567 |
| UniProt ID | Q2KHI9 |
| Gene Summary | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855, PubMed:22771120). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex at double-stranded DNA breaks to generate ssDNA by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (By similarity). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:22771120, PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:22771120, PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (By similarity). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:21987787). Probably by regulating HR, plays a key role during gametogenesis (PubMed:21987787, PubMed:22771120).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220090 | Mcm9 (tGFP-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9), (10ug) |
CNY 9,220.00 |
|
| MR220090 | Mcm9 (Myc-DDK-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9) |
CNY 8,984.00 |
|
| MR220090L3 | Lenti ORF clone of Mcm9 (Myc-DDK-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9) |
CNY 10,260.00 |
|
| MR220090L4 | Lenti ORF clone of Mcm9 (mGFP-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9) |
CNY 10,260.00 |
