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Mcm9 (NM_027830) Mouse Tagged ORF Clone
CAT＃: MR220090
- TrueORF®
Mcm9 (Myc-DDK-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9)
ORF Plasmid: tGFP
"NM_027830" in other vectors (4)
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CNY 8,984.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9030408O17Rik; Gm235; Mcmdc1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220090 representing NM_027830
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGAGAACTACACGAAATGGAAAATATTGTGACGTGGAACCGGTGTCCCGCTCAAACCCCGCCC CATGCCTCCGAGACCCGCCCCTCAGACGTCTTGTCCGGCCCAAACCCCGCCTCCAGCTCCCCGAGTCCCG CCTCTCTCCCTGCTCCCGCCTCCCGCTCGCAGACTCCAGCGTCCGTCCTGGCGCGCGCCCGCCTGCGTCC GCACCCGGAAGAAGCCCCAGCGGCCGGAAAGTTGAGGCAGTGCGCGGCTCGGGGTCCGCGGGAAGCTCAA GTCCTTCAGAGGCCGAGCGAGAGCAGCGCGAAGAAGCCTGCGCGCCCCCGCGCAAGGCGGCCCCCTCGAG CGGCCGCGCGCACGCCCCGCCCCCTCCAACGCCGCGCGGGTCGGGCTGGGGCGACCACGGCCGCAGCGCG GTCCCGGCGACCAAGACAGTGCGTGTTGAGCCCTACCCACCCTTCAAGATGAATAGTGAGCAGGTCACCC TGGTGGGTCAGGTGTTTGAGTCCTATGTTTCAGAGTACCATAAGAACGATATTCTTCTGATCCTGAAAGA AAGAGATGAAGATGCTCACTACCCGGTTGTGGTTAATGCTATGAGCCTTTTCGAGACCAACATGGAAATT GGGGACTATTTCACCGTGTTCCCCAATGAAGTACTAACAGTTTTTGACAGTGCACTTCGAAGGTCAGCCT TGGCAATTCTGCAGTCCCTTCCTGAGACGGAGGGGTTATCCATGAAGCAGAATCTTCATGCCAGGATATC AGGTTTGCCTGTTTGTCCAGAACTGGTCAGGGAACACATTCCCAAAACCAAGGATGTGGGACACTTCTTA TCTGTCACTGGGACAGTGATCCGAACGAGTCTGGTGAAGGTCTTGGAGTTCGAGCGGGATTACATGTGTA ACAAATGCAAGCATGTGTTCATGGTGGAGGCAGACTTCGAGCAGTATTACACCTTCAGTCGGCCATCGTC ATGTCCAAGTTTAGCCAGCTGTGACTCCTCAAAATTCTCTTGCCTCTCAGACTTGTCTTCATCTCCAGCC AGATGTCGGGATTACCAGGAAATCAAAATTCAGGAGCAGGTGCAAAGGCTGTCTGTTGGAAGTATCCCAC GGTCTATGAAAGTTATTCTGGAAGATGACCTAGTTGACAGTTGCAAATCTGGAGATGACCTCACCATCTA TGGGGTTGTAATGCAACGGTGGAAACCCTTTCAGCGAGATGTACGCTGTGAAGTTGAGATTGTCTTGAAA GCCAACTATGTCCAAGTGAATAATGAGCAATCCTCGGGGATGGTCATGGATGAGGACACTCGAAAAGAAT TTGAAGACTTCTGGGAACACTATAAGAGTGACCCCTTTGCAGGGAGGAATGAAATATTGGCCAGCTTGTG TCCTCAAGTTTTTGGGATGTATCTAGTGAAGCTTGCTGTGGCCATGGTACTGGCTGGTGGAATTCAAAGA ACTGATGCTGCAGGAACCAGGGTTAGAGGGGAATCTCACCTTTTATTGGTTGGGGATCCTGGCACAGGGA AATCACAATTCCTTAAATATGCAGCAAAGATTACCCCAAGGTCCGTTTTGACCACAGGAATTGGATCTAC TAGTGCAGGTCTGACCGTGACAGCTGTCAAAGACTCAGGAGAATGGAATCTAGAGGCTGGGGCTTTGGTG CTTGCAGATGCTGGTCTCTGCTGTATTGACGAATTTAACAGCCTCAAAGAACATGACAGGACAAGCATCC ATGAAGCAATGGAGCAACAAACCATAAGTGTTGCTAAGGCTGGCCTTGTTTGTAAGCTGAACACAAGGAC CACCATCCTGGCAGCAACTAACCCCAAAGGCCAGTATGACCCCAAGGAGTCTGTGTCTGTGAACATTGCC CTCGGGAGCCCACTCTTAAGTCGATTTGACCTTGTCCTGGTTTTGCTCGACACTAGGAATGAAGACTGGG ATCGTATCATTTCTTCCTTTATCTTAGAAAATAAAGGTTATCCAAGCAAATCAGAGAATCTGTGGAGCAT GGAGAAGATGAAAACCTACTTCTGCCTCATTCGGAATCTCCACCCCACACTGTCTGAAGTGAGCAATCAA GTCCTCCTTCGATACTACCAAATGCAAAGGCAGAGTGATTCCCGGAATGCAGCCCGGACAACCATCCGCC TGTTAGAAAGCTTGATCCGATTAGCAGAAGCTCACGCTCGCCTGATGTTCCGCAGTGCCGTGACTCTGGA AGATGCCGTTACAGCTGTATCTGTGATGGAGTCTTCAATGCAGGGAGGTGCTCTGCTAGGAGGTGTGAAT GCTCTCCACACTTCCTTCCCTGAAAACCCCCGTGCACAGTACCAGAGGCAGTGTGAACTCATTCTGGAAA AGCTGGAACTCCAGGGCCTCTTGCAGGAAGAACTTCGAAGACTGGAAAGGTTACAGAATGAGAGTGTACA CCAATGCCAGTCACATTCACTAGAGGAGGAGGTGGCTCCAGGTTCCTGCAGAAATGATCCCAGGGACAAG CCAAGGCTCAGGACTTCAACACAGCAGGAACAGAGCTGTAGCTGGAGTTCCACAGAGAGATCTGGTGCAG ACTCCCCGCCTGGTCCAGGGCTTAATAGACCAACAAGTTGTAACAACTCAGCTGAGAACAGAGATGGCAG AGGTGACGGTTTAGACTGGTTGGACCCCACATCAAGTCCTGAGATTGCACCAGAAAGCACTATTGTGTCT CCCAATGTGAAAACAACTGAGAAAAATGTGAATTTGAAAATCTCCAACAATAAATCTCAGGGCAAGGAGA AGCATGGGCCACAGCAAAGAAGCAAATTATTAGAAGCTGGACATCTTCCATCATCAGGAGCCATGAATGC CCCCTTACGGTCTCACGGTGTTAAGCGTACAAAGGCAAGTCAGGCAGTGGTTGTATCTGAAGCAGGACGG GGTGATGAAGAAGACTCTGTGCCCCGAAGACTCCCCAAGCTGCTGAAGGAGGGGTCACAGAATGTGTGCA GAAGCACAACCAGAGTGCGACCACTGCCACCCACTGTCCCTCTGTCCCTGTCTATCCCTTCACCTGGGTC AGGAAAAAGATCAGGAACACCCAAAAGAAAGAGACGGAAATCTGCTCAGGTGGAAGAGCCTGAACCTGAA GGTATGGAGACTCCAACAGTAAAGCTGGCCAAATTCACATTTAAACAGAAGACAAAACTGACCCACTCCC CCGAAGGCCAAGGCCCCATACCTCCCAGTGCTAGTGAAATAGCAGTTGACAGTTCTAAAATTCCCCAACA AAGAACAAGAAGAGAAGCAGCTGTGCCTGTGGTAGCGCCAGGAAAGTCGACATCCACCTCAGGAGACAGA TGCTCTGACCAGCTGCACGGAAAGACAAAGGAGCTCTCAAGGCAGCCACCAGACAGTAATCCACCAAGAG AGGAGAGGGAACAGGGCCCCAAAAGAAGGGTTATTCAGCCCAAACCGGAGCTTGGGAACCAGGCTGGGCA TTCGCATCTAGCCTGTGAGAAAGACAGAAAGGAAGGGGTTTCCTGCGGTAATAAAAGCAGCAAGGTTCAT GCTGGCACCATAGCCAGACTGGCAAGCTTCTCCTTCACTTCCCCATCAGAATCCAAATCGGAATCCCTCC CTCCTGAAAGGAAGGACAGCAGGGACAGCAGGGACAGCAGGGACAGCAGGGACAGGTGCCACAGCCCTCC TGCGACCACAGCTCCAGTGCTCGGCCAGCAAAGGCAGACTTTCCAGCTGCAGCAGCCCACAGAGAGAGCG AATCTTTCCACCCTCTCCCTCTTCACTCTGTCGGAACTAGATGACGAAGCATTAGATTTTGACTGGGAGG AAGAGATGAGGAAAAAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220090 representing NM_027830
Red=Cloning site Green=Tags(s) MDQRTTRNGKYCDVEPVSRSNPAPCLRDPPLRRLVRPKPRLQLPESRLSPCSRLPLADSSVRPGARPPAS APGRSPSGRKVEAVRGSGSAGSSSPSEAEREQREEACAPPRKAAPSSGRAHAPPPPTPRGSGWGDHGRSA VPATKTVRVEPYPPFKMNSEQVTLVGQVFESYVSEYHKNDILLILKERDEDAHYPVVVNAMSLFETNMEI GDYFTVFPNEVLTVFDSALRRSALAILQSLPETEGLSMKQNLHARISGLPVCPELVREHIPKTKDVGHFL SVTGTVIRTSLVKVLEFERDYMCNKCKHVFMVEADFEQYYTFSRPSSCPSLASCDSSKFSCLSDLSSSPA RCRDYQEIKIQEQVQRLSVGSIPRSMKVILEDDLVDSCKSGDDLTIYGVVMQRWKPFQRDVRCEVEIVLK ANYVQVNNEQSSGMVMDEDTRKEFEDFWEHYKSDPFAGRNEILASLCPQVFGMYLVKLAVAMVLAGGIQR TDAAGTRVRGESHLLLVGDPGTGKSQFLKYAAKITPRSVLTTGIGSTSAGLTVTAVKDSGEWNLEAGALV LADAGLCCIDEFNSLKEHDRTSIHEAMEQQTISVAKAGLVCKLNTRTTILAATNPKGQYDPKESVSVNIA LGSPLLSRFDLVLVLLDTRNEDWDRIISSFILENKGYPSKSENLWSMEKMKTYFCLIRNLHPTLSEVSNQ VLLRYYQMQRQSDSRNAARTTIRLLESLIRLAEAHARLMFRSAVTLEDAVTAVSVMESSMQGGALLGGVN ALHTSFPENPRAQYQRQCELILEKLELQGLLQEELRRLERLQNESVHQCQSHSLEEEVAPGSCRNDPRDK PRLRTSTQQEQSCSWSSTERSGADSPPGPGLNRPTSCNNSAENRDGRGDGLDWLDPTSSPEIAPESTIVS PNVKTTEKNVNLKISNNKSQGKEKHGPQQRSKLLEAGHLPSSGAMNAPLRSHGVKRTKASQAVVVSEAGR GDEEDSVPRRLPKLLKEGSQNVCRSTTRVRPLPPTVPLSLSIPSPGSGKRSGTPKRKRRKSAQVEEPEPE GMETPTVKLAKFTFKQKTKLTHSPEGQGPIPPSASEIAVDSSKIPQQRTRREAAVPVVAPGKSTSTSGDR CSDQLHGKTKELSRQPPDSNPPREEREQGPKRRVIQPKPELGNQAGHSHLACEKDRKEGVSCGNKSSKVH AGTIARLASFSFTSPSESKSESLPPERKDSRDSRDSRDSRDRCHSPPATTAPVLGQQRQTFQLQQPTERA NLSTLSLFTLSELDDEALDFDWEEEMRKKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027830 |
| ORF Size | 3870 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027830.2, NP_082106.2 |
| RefSeq Size | 3873 bp |
| RefSeq ORF | 3873 bp |
| Locus ID | 71567 |
| UniProt ID | Q2KHI9 |
| MW | 142.4 kDa |
| Gene Summary | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855, PubMed:22771120). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex at double-stranded DNA breaks to generate ssDNA by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (By similarity). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:22771120, PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:22771120, PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (By similarity). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:21987787). Probably by regulating HR, plays a key role during gametogenesis (PubMed:21987787, PubMed:22771120).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224169 | Mcm9 (untagged) - Mouse minichromosome maintenance complex component 9 (Mcm9), (10ug) |
CNY 16,630.00 |
|
| MG220090 | Mcm9 (tGFP-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9), (10ug) |
CNY 9,220.00 |
|
| MR220090L3 | Lenti ORF clone of Mcm9 (Myc-DDK-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9) |
CNY 10,260.00 |
|
| MR220090L4 | Lenti ORF clone of Mcm9 (mGFP-tagged) - Mouse minichromosome maintenance complex component 9 (Mcm9) |
CNY 10,260.00 |
