Tcof1 (NM_011552) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA408847; AW209012; treacle
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224234 representing NM_011552 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCCAGGAAGCGGCGGGAGCTGCTTCCCCTCATTTACCATCATCTGTTGCAAGCAGGCTACG TCCGCGCGGCGCGGGAAGTAAAGGAGCAGAGCGGCCAGAAGAGTTTCCTGACTCAGCCCGTCACCCTTCT GGACATCTATACACACTGGCAACAGACCTCAGAGCTTGGCCAGAAGCAGAAGGCAGAGGATGATGAGACC CTTCAGGCTAAGAAGTCTCGAGTGTCGGATCCTGTTAGCAGCTCAGAGAGCTCGGATCAGGAGAAGGAAG AGGAGGCAGCAACCGAAAGGGCCAAAGCCACCCCAAGACCGACACCTGTCAATTCTGCAACCGCAGCTTT GCCATCAAAAGTAAAAGAAAAGGGAAAGACCAAGACAGCCAACAAGACGGTGAACTCTGTGTCGCACCCT GGGTCCGGAAAGACGGTGGTCCACCTGCTCTCTGGGAAGTCACCCAAAAAGTCAGCAGAGCCCTTGGCAA ACACTGTCTTGGCCTCAGAAACTGAGGAGGAGGGCAATGCCCAAGCCCTCGGACCCACTGCCAAGTCTGG AACGGTGTCAGCGGGCCAAGGCAGCAGTTCCAGTGAAGATTCCTCCATCTCAAGCGATGAGACAGATGTC GAGGTGAAATCTCCAGCAAAACCAGCCCAGGCCAAAGCTTCAGCAGCCCCTGCCAAGGATCCTCCAGCAA GAACAGCCCCAGGCCCTACCAAGTTAGGGAATGTGGCGCCCACACCTGCTAAACCAGCCAGGGCGGCAGC GGCAGCTGCTGCTGCTGCTGTGGCTGCTGCTGCTGCTGCAGCAGCAGAAGAGTCTGAGAGCAGTGAGGAG GACTCAGACAGTGAGGACGAGGCCCCTGCTGGTCTGCCCAGCCAGGTAAAAGCCTCTGGAAAAGGTCCCC ATGTCAGAGCCGACTCGGTGTCTGCCAAGGGGATCTCTGGGAAAGGGCCCATCTTAGCAACCCCAGGGAA GACTGGGCCTGCAGCCACCCAGGCCAAGGCAGAAAGGCCAGAGAAGGACTCGGAAACCAGCAGTGAGGAC GATTCTGATAGTGAGGATGAAATGCCAGTCACTGTGAATACTCCTCAGGCAAGGACTTCTGGGAAGAGCC CTCGGGCCAGAGGTACCTCAGCCCCCGCCAAGGAGTCATCCCAGAAAGGGGCTCCTGCAGTCACCCCTGG AAAGGCAAGGCCTGTGGCAGCCCAGGCAGGGAAACCAGAAGCCAAGAGCAGTGAGGAGTCAGAGAGTGAC AGTGGGGAGACACCAGCTGCTGCGACTCTGACCACGAGTCCTGCCAAGGTGAAACCTTTGGGGAAGAGCT CCCAGGTCAGACCTGTTTCCACCGTCACCCCGGGGTCATCGGGAAAAGGTGCCAACCTGCCCTGCCCTGG GAAGGTGGGGTCAGCAGCTCTCAGGGTCCAAATGGTAAAGAAAGAAGATGTCTCGGAGAGCAGCAGTGCA GAGCTGGACAGTGACGGGCCTGGGAGCCCAGCCAAGGCAAAGGCCTCCCTTGCGCTCCCTCAGAAGGTGA GGCCTGTGGCCACCCAGGTCAAGACTGACAGGGGCAAAGGCCACTCAGGGAGCAGTGAGGAGTCATCTGA CAGCGAAGAGGAGGCAGCACCAGCAGCCTCTGCTGCTCAGGCTAAGCCAGCTCTGGAAAAGCAGATGAAA GCTTCCTCTAGGAAAGGCACGCCTGCATCCGCAACAGGAGCGAGCACCTCGTCCCATTGTAAGGCAGGAG CGGTGACCTCTTCAGCCAGCCTGTCATCCCCAGCTCTGGCCAAGGGCACCCAGAGGTCAGATGTGGACTC TTCCAGTGAGTCTGAGTCAGAAGGAGCTGCTCCCAGCACCCCCAGGGTACAGGGGAAGTCTGGGGGCAAG GGCCTCCAAGGGAAAGCTGCCTTGGGGCAAGGGGTGGCCCCAGTGCACACTCAGAAGACAGGGCCTTCGG TCAAAGCTATGGCTCAGGAAGACTCAGAGAGCCTCGAGGAGGACTCCAGCAGTGAGGAAGAGGATGAGAC CCCAGCACAGGCCACGCCCTTGGGGAGACTTCCTCAGGCCAAAGCCAACCCACCTCCCACTAAGACACCT CCAGCGTCTGCATCTGGAAAAGCTGTGGCTGCTCCAACCAAGGGAAAACCACCTGTTCCGAACAGCACCG TCTCTGCAAGGGGCCAGCGGTCTGTGCCAGCCGCGGGAAAAGCAGGGGCCCCAGCAACCCAAGCCCAGAA GGGTCCCGTGGCTGGCACAGGGGAGGACTCAGAGAGCAGCAGTAAAGAGGAGTCTGACAGTGAAGAAGAG ACGCCAGCCCAGATAAAACCTGTGGGGAAGACCTCTCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGAGT CTCCTAAAAAAGGAGCCCATCCAGGAACCCCCGGCAAGACGGGATCTTCAGCTACCCAGGCCCAGCCAGG GAAGACAGAGGACTCAGACAGCAGTAGTGAAGAGTCTGACAGTGACACAGAGATGCCATCAGCCCAGGCG ATTAAATCCCCTCCAGTTTCTGTCAACCGTAACAGTAGTCCAGCTGTCCCAGCTCCTACCCCAGAAGGAG TCCAGGCTGTGAACACCACAAAGAAGGCCTCAGGCACCACTGCCCAGAGCTCCTCCTCTGAGAGTGAGGA CGGGGACGAGGACTTGATTCCTGCCACACAACCCTCCACCTATGCTCTCAGAACCAGTGTGACGACGCCC GCAGCCCTCTCACGAGCAGCTTCCCAACCCAGCAAGAGTGAGCAGTCTAGCCGGATGCCAAAAGGCAAGA AAGCAAAGGCGGCGGCGTCCGCTCAGACCAGCAGTGCCGTGGAAACACTCCCCATGATGCCTCCCCAGAG CGCACCCATCCAGCCCAAAGCCACCAACAAGCTCGGGAAATCCAAGCTCCCTGAGAAGCAGCAGCTTGCC CCAGGCTACCCCAAGGCCCCCAGGAGCTCGGAGGACAGCAGTGACACTTCTTCCGAGGACGAGGAGGATG CCAAGAGACCCCAGATGCCCAAGTCGGCCCACAGGCTGGATCCAGACCCTTCCCAGAAGGAAACTGTGGT AGAGGAGACCCCTACAGAATCCAGCGAAGATGAGATGGTGGCCCCCTCACAGTCTCTCCTCTCAGGTTAC ATGACTCCCGGCTTGACTGTGGCTAATTCCCAGGCTTCAAAAGCTACTCCTAGGCCAGACTCCAACTCCT TAGCTTCTTCTGCCCCGGCCACCAAAGACAACCCGGATGGCAAGCAGAAGTCAAAATCCCAACACGCAGC AGACACCGCACTCCCTAAAACCGGTAGGAAAGAGGCCTCCTCGGGCTCCACGCCTCAGAAGCCCAAGAAG CTCAAGAAGAGCACCTCAAGCTCGCCGGCCCCCACACAGACACTGCCGAACAGCATCACCCAGCGCCTCC TGGAGCAGGCCTGGCCCCTGAGCGAGGCGCAGGTGCAGGCCTCTGTGGTGAAGGTCCTGACAGAGCTTCT GGAGCAGGAACGGCTGAAGGCCACAGAGGCCATCAAGGAGAGTGGGAAGAAGAGCCAGAAGCGGAAGTTA TCAGGGGACCTAGAAGCTGGGGCCCCAAAGAACAAGAAGAAGAAGGAGCAGCCAGTGCCCAGGGCAAGCG CTGTTTCCCCAGAAAAGGCTCCCATGACTTCCAAGGCAAAATCAAAGCTTGACAAAGGGAGTGCTGGTGG CAAGGGGAAGGGGTCTCCTGGCCCCCAAGGAGCCAAGGAGAAGCCCGACGGCGAGTTGCTGGGGATAAAG CTTGAGAGTGGCGAGCAGAGCGACCCGAAGAGCAAGTCGAAAAAGAAGAAATCCCTCAAGAAAAAAAAAG ACAAGGAGAAAAAGGAAAAGAAGAAAGGAAAAAAGTCCCTGGCCAAAGACTCTGCCTCGCCGATCCAGAA GAAGAAAAAGAAGAAGAAGAAGTCAGCCGAGCCTGCCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011552
Insert Size	3963 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011552.3, NP_035682.1
RefSeq Size	4571 bp
RefSeq ORF	3963 bp
Locus ID	21453
UniProt ID	O08784
Gene Summary	Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame alternate exon in the 3' coding region, compared to variant 1, and encodes a shorter isoform (2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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