Col1a1 (NM_007742) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Co; Col; Col1; Col1a-1; Cola-1; Cola1; Mov-; Mov-13; Mov13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224478 representing NM_007742 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTTTGTGGACCTCCGGCTCCTGCTCCTCTTAGGGGCCACTGCCCTCCTGACGCATGGCCAAG AAGACATCCCTGAAGTCAGCTGCATACACAATGGCCTAAGGGTCCCCAATGGTGAGACGTGGAAACCCGA GGTATGCTTGATCTGTATCTGCCACAATGGCACGGCTGTGTGCGATGACGTGCAATGCAATGAAGAACTG GACTGTCCCAACCCCCAAAGACGGGAGGGCGAGTGCTGTGCTTTCTGCCCGGAAGAATACGTATCACCAA ACTCAGAAGATGTAGGAGTCGAGGGACCCAAGGGAGACCCTGGCCCCCAAGGCCCAAGGGGACCCGTTGG CCCCCCTGGACGAGATGGCATCCCTGGACAGCCTGGACTTCCTGGTCCTCCTGGTCCCCCTGGGCCCCCC GGACCCCCTGGCCTTGGAGGAAACTTTGCTTCCCAGATGTCCTATGGCTATGATGAAAAATCAGCTGGAG TTTCCGTGCCTGGCCCCATGGGTCCTTCTGGTCCTCGTGGTCTCCCTGGCCCCCCTGGTGCACCTGGTCC ACAAGGTTTCCAAGGCCCCCCTGGTGAACCTGGCGAGCCTGGCGGTTCAGGTCCAATGGGTCCCCGAGGT CCCCCTGGCCCTCCTGGCAAGAATGGAGATGATGGGGAAGCTGGCAAGCCCGGCCGTCCTGGTGAGCGTG GACCTCCTGGACCTCAGGGTGCTCGTGGATTGCCTGGAACAGCTGGCCTCCCTGGAATGAAGGGACACCG AGGCTTCAGTGGTTTGGATGGTGCCAAAGGAGATGCTGGTCCTGCTGGTCCTAAGGGAGAGCCCGGCAGT CCTGGTGAAAACGGAGCTCCTGGCCAGATGGGTCCCCGAGGTCTGCCCGGTGAGAGAGGTCGCCCTGGAC CTCCTGGCACTGCTGGTGCTCGCGGTAACGATGGTGCTGTTGGTGCTGCTGGACCCCCTGGTCCCACCGG CCCCACTGGCCCTCCTGGCTTCCCTGGTGCAGTTGGTGCTAAGGGTGAAGCTGGTCCCCAAGGAGCTAGA GGCTCTGAAGGTCCCCAGGGTGTGCGTGGTGAGCCCGGACCCCCTGGCCCTGCTGGTGCTGCCGGCCCTG CTGGAAACCCTGGTGCTGATGGACAACCTGGCGCTAAAGGTGCCAATGGTGCTCCTGGTATTGCTGGTGC TCCTGGCTTCCCTGGTGCCCGAGGCCCCTCTGGACCCCAGGGCCCCAGCGGCCCTCCAGGTCCCAAGGGT AACAGTGGTGAACCTGGTGCTCCTGGCAACAAAGGAGACACTGGTGCCAAAGGAGAACCCGGTGCTACTG GAGTTCAAGGTCCCCCAGGCCCTGCCGGAGAAGAAGGAAAACGAGGAGCCCGTGGTGAGCCTGGACCTTC CGGACTGCCTGGACCTCCTGGCGAGCGTGGTGGACCTGGTAGCCGTGGTTTCCCTGGTGCTGATGGTGTT GCTGGCCCCAAGGGTCCTTCCGGTGAACGTGGTGCTCCCGGACCTGCTGGTCCCAAAGGTTCTCCTGGTG AAGCTGGTCGCCCCGGTGAAGCTGGTCTCCCTGGTGCCAAGGGTCTCACTGGCAGTCCTGGCAGCCCTGG TCCTGATGGCAAAACCGGCCCCCCTGGTCCCGCTGGTCAAGATGGTCGCCCTGGACCCGCAGGTCCTCCT GGAGCCCGTGGCCAGGCTGGTGTGATGGGATTCCCTGGACCTAAGGGTACCGCTGGAGAACCTGGAAAGG CTGGAGAGCGAGGCCTTCCCGGACCCCCTGGCGCTGTTGGTCCTGCTGGCAAAGATGGAGAAGCTGGAGC TCAGGGAGCCCCTGGCCCTGCTGGTCCTGCTGGTGAGAGAGGTGAACAAGGTCCCGCTGGCTCCCCTGGA TTCCAGGGTCTTCCTGGTCCTGCCGGTCCTCCTGGTGAAGCAGGCAAGCCTGGTGAACAGGGTGTTCCTG GAGACCTTGGTGCCCCTGGACCCTCTGGCGCAAGAGGCGAGAGAGGTTTCCCTGGTGAACGTGGTGTACA AGGTCCCCCAGGTCCTGCTGGTCCCCGAGGAAACAATGGTGCCCCCGGCAACGATGGTGCCAAGGGTGAT ACTGGTGCCCCCGGAGCTCCCGGTAGCCAGGGTGCCCCCGGTCTTCAGGGAATGCCTGGTGAACGTGGTG CAGCTGGTCTTCCAGGTCCTAAGGGTGACAGAGGTGATGCTGGTCCCAAAGGTGCTGATGGTTCTCCTGG TAAAGATGGTGCCCGTGGTCTGACTGGTCCCATTGGTCCTCCTGGCCCTGCTGGTGCCCCTGGTGACAAG GGTGAAGCTGGTCCCAGTGGTCCTCCCGGTCCCACCGGAGCCCGTGGTGCTCCCGGAGACCGTGGTGAGG CTGGTCCCCCTGGTCCTGCTGGCTTTGCCGGCCCCCCTGGTGCTGATGGCCAACCTGGTGCGAAAGGTGA ACCTGGTGATACTGGTGTTAAAGGTGATGCTGGTCCTCCTGGCCCTGCTGGTCCTGCTGGACCCCCCGGC CCCATTGGTAACGTTGGTGCTCCTGGACCCAAAGGTCCTCGTGGTGCTGCTGGTCCCCCTGGTGCTACTG GCTTCCCTGGTGCTGCTGGCCGTGTCGGTCCCCCTGGTCCCTCTGGAAATGCTGGACCCCCTGGCCCTCC CGGTCCCGTTGGCAAAGAAGGGGGCAAAGGTCCCCGTGGTGAGACTGGCCCTGCTGGACGTCCTGGTGAA GTTGGTCCCCCAGGTCCCCCCGGTCCTGCTGGTGAGAAAGGATCTCCTGGTGCTGATGGACCTGCTGGCT CTCCTGGTACCCCTGGACCTCAGGGTATTGCTGGACAACGTGGTGTGGTCGGTCTTCCCGGTCAGAGAGG AGAAAGAGGCTTCCCTGGTCTTCCTGGCCCCTCTGGTGAACCTGGCAAACAAGGTCCTTCTGGATCAAGT GGTGAACGCGGTCCCCCTGGCCCCATGGGGCCCCCTGGATTGGCTGGTCCCCCTGGTGAATCTGGACGTG AGGGATCCCCTGGTGCTGAAGGCTCCCCTGGAAGGGATGGTGCTCCCGGGGCCAAGGGTGACCGTGGTGA GACTGGCCCCGCTGGCCCCCCTGGTGCCCCTGGTGCTCCCGGTGCTCCCGGCCCTGTTGGTCCCGCTGGC AAGAATGGCGATCGTGGTGAGACTGGTCCTGCTGGTCCTGCTGGTCCCATTGGCCCTGCTGGTGCCCGTG GCCCTGCTGGACCCCAAGGCCCCCGTGGTGACAAGGGTGAGACAGGCGAACAAGGTGACAGAGGCATAAA GGGTCATCGTGGCTTCTCTGGTCTCCAGGGTCCTCCTGGTTCTCCTGGTTCTCCTGGTGAACAAGGCCCC TCTGGAGCTTCAGGTCCTGCAGGCCCCCGGGGTCCCCCTGGCTCTGCTGGTTCTCCTGGCAAAGACGGAC TCAACGGTCTCCCTGGCCCCATTGGTCCCCCTGGTCCTCGAGGTCGCACTGGTGACAGCGGCCCTGCTGG TCCCCCCGGCCCTCCTGGACCCCCTGGCCCTCCTGGACCTCCCAGTGGCGGTTATGACTTCAGCTTCCTG CCTCAGCCACCTCAAGAGAAGTCTCAAGATGGTGGCCGCTACTACCGGGCCGATGATGCTAACGTGGTTC GTGACCGTGACCTTGAGGTGGACACCACCCTCAAGAGCCTGAGTCAGCAGATTGAGAACATCCGCAGCCC CGAAGGCAGCCGCAAGAACCCTGCCCGCACATGCCGCGACCTCAAGATGTGCCACTCTGACTGGAAGAGC GGAGAGTACTGGATCGACCCTAACCAAGGCTGCAACCTGGACGCCATCAAGGTCTACTGCAACATGGAGA CAGGTCAGACCTGTGTGTTCCCTACTCAGCCGTCTGTGCCTCAGAAGAACTGGTACATCAGCCCGAACCC CAAGGAAAAGAAGCACGTCTGGTTTGGAGAGAGCATGACCGATGGATTCCCGTTCGAGTACGGAAGCGAG GGCTCCGACCCCGCCGATGTCGCTATCCAGCTGACCTTCCTGCGCCTAATGTCCACCGAGGCCTCCCAGA ACATCACCTATCACTGCAAGAACAGCGTAGCCTACATGGACCAGCAGACTGGCAACCTCAAGAAGGCCCT GCTCCTCCAGGGATCCAACGAGATCGAGCTCAGAGGCGAAGGCAACAGTCGCTTCACCTACAGCACCCTT GTGGACGGCTGCACGAGTCACACCGGAACTTGGGGCAAGACAGTCATCGAATACAAAACCACCAAGACCT CCCGCCTGCCCATCATCGATGTGGCTCCCTTGGACATTGGTGCCCCAGACCAGGAATTCGGACTAGACAT TGGCCCTGCCTGCTTCGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007742
Insert Size	4362 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007742.4, NP_031768.2
RefSeq Size	5946 bp
RefSeq ORF	4362 bp
Locus ID	12842
UniProt ID	P11087
Gene Summary	This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing. [provided by RefSeq, Nov 2015]

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