Myh11 (NM_001161775) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV071570; SM1; SM2; smMHC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224592 representing NM_001161775 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGAAAGGGCAGCTCAGCGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATGAACAGCC CAATGGCTCAGGCCGACTGGGTAGCCAAGAAGCTGGTGTGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTCGTGGAGTTGGTGGAAAATGGAAAGAAGGTCACA GTTGGCAAAGATGACATCCAAAAAATGAACCCACCCAAGTTCTCTAAGGTGGAGGACATGGCAGAGCTGA CGTGCCTCAATGAGGCCTCTGTGCTGCACAACCTGAGGGAGCGATACTTCTCAGGCCTCATCTATACCTA CTCTGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGTACCTACCCATCTACTCAGAAAAGATCGTGGAT ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCCGACACAGCCTACAGAA GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCCGGAAAGACAGAGAA CACAAAGAAAGTCATACAGTACTTGGCTGTGGTGGCATCCTCCCACAAGGGCAAGAAAGACAGCAGCATC ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCGAAAACCG TCAAGAACGACAACTCCTCTCGCTTTGGCAAGTTCATTCGCATCAACTTCGATGTCACTGGTTACATTGT AGGTGCCAATATTGAAACATATCTTCTGGAAAAGTCTAGGGCTATTCGACAGGCTAGGGATGAGAGAACA TTTCACATCTTCTACTACCTGCTCGCCGGAGCCAAGGAAAAGATGAAAAGTGACCTGCTTTTGGAGAGCT TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA GGAGACACTGGAAGCCATGTCTATCATGGGCTTCAATGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA TCATCTGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG ATAACACAGCGGCTCAGAAAGTTTGCCACCTCGTGGGGATTAATGTGACAGATTTCACTAGAGCCATCCT GACCCCACGTATCAAAGTTGGACGGGATGTGGTGCAGAAGGCTCAGACCAAAGAACAGGCTGACTTTGCC ATCGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTAAACAAGGCCT TGGACAAGACCCATCGGCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCTGGGTTTGAAATCTTTGA GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACG ATGTTCATCCTGGAGCAGGAAGAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTCGGCCTGG ACCTGCAGCCTTGTATTGAGCTGATTGAGCGGCCGAACAACCCTCCCGGTGTGCTGGCCCTGCTGGATGA AGAATGCTGGTTCCCCAAAGCTACAGACAAGTCTTTTGTGGAGAAGCTATGCTCAGAGCAGGGAAATCAC CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG TGGACTACAATGCAAGTGCCTGGCTGACCAAGAACATGGACCCGCTAAATGACAATGTGACATCACTCCT CAATGCCTCCTCTGACAAGTTTGTGGCTGACCTGTGGAAGGATGTGGACCGCATTGTGGGGCTGGACCAG ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG GCCAGCTCTACAAAGAGCAGTTGGGGAAACTGATGACTACACTGCGCAATACCACGCCTAACTTTGTGCG CTGCATCATCCCCAACCATGAGAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTGCTGGAACAGCTGCGC TGCAACGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT TCCGGCAACGCTATGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGATGGAAAGCAAGCCTG CATTCTCATGATCAAAGCCCTCGAACTTGACCCTAACCTGTACAGGATTGGGCAGAGCAAAATCTTCTTC CGAACGGGGGTCCTGGCCCACCTAGAGGAGGAACGAGACCTGAAAATTACTGATGTCATCATGGCCTTCC AGGCAATGTGTCGTGGCTACCTTGCCAGAAAGGCCTTCACCAAGAGGCAGCAACAGCTGACAGCCATGAA GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGCGGCTCTTCACCAAA GTAAAGCCATTGCTCCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAAAAGA TCAAGGAGCGGCAGCAGAAGGCAGAGACTGAGTTGAAGGAGCTGGAGCAGAAGCACACTCAGCTGGCTGA GGAGAAGACTCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAGGAGATGCGG GTCCGGTTGGCAGCCAAGAAGCAGGAACTGGAGGAGATCCTACATGAGATGGAGGCCCGCCTGGAGGAAG AGGAAGACCGGGGCCAGCAACTACAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTAGACCTGGAGGA GCAACTGGAGGAGGAAGAGGCCGCCAGACAGAAACTACAGCTAGAGAAGGTCACGGCTGAGGCCAAGATC AAGAAACTGGAGGATGACATCTTGGTTATGGATGATCAGAACAGTAAACTTTCAAAAGAACGAAAACTCC TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA GCTGAAGAGCAAGCATGAGTCTATGATCTCAGAGCTGGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCATGAGCAGATCGCTGACT TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC CAGGCTTGATGAAGAGATCGCCCAGAAAAACAATGCCCTAAAGAAGATTCGCGAGCTAGAGGGCCATATC TCAGACCTACAAGAGGACCTAGACTCAGAGCGGGCTGCCAGGAACAAGGCCGAGAAGCAGAAGCGAGACC TGGGGGAGGAGCTGGAGGCACTCAAGACGGAGCTGGAGGATACGCTGGACAGCACAGCTACCCAGCAGGA GCTCAGAGCCAAGAGGGAACAGGAGGTGACAGTGCTGAAGAAGGCCCTGGATGAGGAGACGCGGTCCCAT GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAGC AGTTCAAAAGGGCCAAGGCAAACCTGGACAAAAGCAAGCAGACACTGGAGAAGGAGAACGCGGACCTGGC TGGGGAGCTGCGTGTCCTGGGCCAGGCGAAGCAGGAGGTGGAACACAAGAAGAAGAAGCTGGAGGTGCAG CTGCAGGATCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGGCTGAGCTCAGTGACAAGGTCCACA AGCTACAGAATGAAGTGGAGAGTGTCACTGGCATGCTCAATGAGGCAGAGGGCAAAGCCATCAAACTGGC CAAAGATGTGGCTTCCCTTGGATCCCAGCTTCAGGACACCCAAGAGCTGCTCCAAGAAGAAACCCGGCAG AAGCTCAATGTGTCTACCAAGCTGCGTCAGTTGGAAGATGAAAGGAACAGCCTGCAGGACCAGCTGGATG AGGAGATGGAGGCTAAGCAAAACCTGGAGCGCCATGTCTCAACACTGAACATTCAGCTCTCAGACTCTAA GAAGAAGCTGCAGGACTTTGCAAGTACCATCGAGGTCATGGAGGAGGGGAAGAAGAGGTTACAGAAAGAG ATGGAGGGCCTCAGCCAGCAGTATGAGGAGAAGGCGGCTGCCTATGACAAACTGGAGAAAACCAAGAACA GGCTCCAGCAGGAGCTGGATGACCTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA AAAGAAGCAGAAGAAATTTGACCAGTTGTTAGCTGAGGAGAAGAACATCTCCTCCAAGTATGCGGATGAG AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAGACAAAGGCTTTGTCTCTAGCCCGGGCCCTGGAGG AAGCCCTGGAAGCCAAAGAAGAGCTGGAGAGGACCAACAAGATGCTCAAAGCTGAGATGGAAGACCTGGT CAGCTCCAAGGATGATGTAGGCAAGAACGTGCATGAACTGGAGAAGTCCAAGCGTGCCTTGGAGACCCAG ATGGAAGAGATGAAAACCCAGCTGGAGGAGTTGGAGGATGAGCTGCAGGCCACTGAGGATGCCAAGCTGC GGCTAGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGCGATCTCCAGGCTCGGGATGAACAGAA TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCACGAGTATGAGACAGAACTGGAAGATGAACGGAAG CAGAGAGCTCTGGCGGCGGCAGCTAAGAAGAAGCTGGAAGGGGACCTAAAAGACCTAGAGCTCCAGGCTG ACTCAGCCATCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTGCAGGCTCAGATGAAGGACTT CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGGGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG AAAGCCAAGAGTCTGGAGGCAGACCTCATGCAGCTCCAAGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCCGAGGAGCTGGCTAGCAGCTTGTCAGGAAGGAATACACT GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAACTAGAGGAGGAGCTGGAGGAAGAGCAGGGC AACATGGAGGCCATGAGTGATAGAGTACGCAAGGCCACACTGCAGGCTGAGCAACTGAGCAATGAGCTGG CCACAGAACGCAGCACGGCTCAGAAGAATGAGAGCGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT GCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTCAAGTCCACTGTTGCGGCGCTGGAG GCCAAGATTGCACAGCTGGAGGAGCAGGTTGAACAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGC TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTGCTGCAGGTGGAGGATGAGCGCAAGATGGCAGAGCA GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTGAAGAGGCAGCTGGAAGAGGCAGAG GAGGAGTCCCAGCGCATCAACGCCAACCGCAGGAAGCTGCAGCGGGAGCTAGATGAGGCCACAGAGAGCA ATGAGGCCATGGGCCGTGAGGTGAACGCCCTCAAGAGCAAACTCAGGAGAGGAAACGAGGCTTCATTTGT TCCTTCCAGAAGGGCTGGGGGCCGTAGAGTTATTGAAAACACAGATGGTTCTGAAGAAGAAATGGACGCT CGGGACTCAGACTTCAATGGAACCAAAGCCAGTGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001161775
Insert Size	5919 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001161775.1, NP_001155247.1
RefSeq Size	6593 bp
RefSeq ORF	5919 bp
Locus ID	17880

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