Nisch (NM_022656) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200007D05Rik; 3202002H23Rik; AW494485; I-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224724 representing NM_022656 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCGACACTCAGCTTCGGCCCTGAGCGGGAGGCCGAGCCTGCCAAGGAGGCGCGCGTCGTGG GTTCTGAGCTCGTGGACACGTACACGGTTTATGTCATCCAGGTTACCGATGGCAACCATGAGTGGACGAT CAAACACCGTTACAGTGATTTTCATGACCTACATGAAAAGCTTGTGGCAGAGAGAAAAATTGACAAATCT CTACTTCCACCCAAAAAGATAATTGGGAAGAACTCGAGAAGCTTGGTAGAGAAGAGGGAGAGAGACCTGG AGGTGTACCTCCAGACGCTCCTCACAACCTTCCCTGATGTGGCTCCCAGAGTTCTGGCCCACTTCCTGCA TTTTCACCTCTATGAAGTAAATGGTGTCACTGCAGCACTGGCTGAAGAACTCTTTGAAAAAGGAGAGCAA CTTCTAGGAGCCGGTGAGGTCTTTGCCATCAGGCCCTTGCAGCTCTATGCTATCACAGAACAGCTGCAAC AGGGAAAGCCTACTTGTGCCAGTGGTGATGCCAAGACTGACCTTGGACACATCCTAGACTTCACCTGTCG CCTTAAGTATCTTAAGGTTTCAGGCACAGAAGGACCTTTTGGGACCAGCAATATTAAGGAGCAGCTCTTG CCCTTTGATCTTTCAATATTCAAGTCTCTTCACCAGGTGGAGATAAGTCATTGTGATGCCAAGCATATCC GAGGGCTGGTCACCTCCAAGCCCACTTTAGCCACAATGAGTGTTAGATTCTCAGCAACCTCAATGAAGGA AGTGCTTGCTCCTGAAGCCTCAGAATTTGATGAGTGGGAGCCTGAAGGCACTGCTACCCTCGGAGGCCCT GTGACTGCTATCATCCCAACATGGCAAGCACTGACTACTCTAGACCTGAGCCACAACAGCATCTGTGAGA TCGACGAATCTGTGAAACTGATCCCAAAGATAGAGTACCTGGACCTGAGTCACAATGGACTGCGAGTTGT GGATAACCTGCAGCACCTATACAACCTCGTGCACCTTGACCTGTCCTACAACAAGCTCTCCTCCCTGGAA GGCGTGCACACTAAACTGGGCAATGTCAAAACCCTAAACCTGGCAGGCAACTTCTTAGAGAGTCTGAGCG GTCTGCACAAACTCTACTCCTTGGTTAATGTGGACCTACGAGACAACCGGATTGAGCAGTTGGATGAGGT CAAGAGCATTGGCAGCCTGCCATGTCTGGAGCGTTTGACCTTGCTGAACAACCCTTTGAGCATCATCCCT GACTACCGGACCAAGGTGCTTTCCCAGTTTGGAGAACGAGCCTCTGAGATTTGTCTAGATGATGTCGCAA CCACAGAGAAAGAGCTGGACACTGTGGAAGTGCTGAAGGCAATTCAGAAAGCCAAAGATGTCAAGTCCAA ACTGAGCAACACAGAAAAGAAGGCTGGTGAGGACTTCCGGCTCCCGCCTGCACCCTGCATCAGACCCGGC GGCTCCCCTCCTGCAGCTCCCGCCTCAGCCTCCCTGCCTCAGCCGATCCTCTCCAACCAAGGTATCATGT TTGTACAGGAAGAGGCCCTGGCCAGCAGCCTCTCATCCACGGATAGTCTGCCACCCGAGGACCACCGGCC CATTGCCCGAGCCTGCTCTGACTCCTTGGAATCTATCCCTGCAGGACAGGTGGCCTCTGATGATCTGAGG GATGTGCCAGGAGCTGTTGGCGGCGTGAGCCCAGATCATGCAGAGCCAGAGGTTCAGGTGGTGCCTGGGT CCGGCCAGATCATCTTCCTGCCCTTCACTTGCATTGGCTACACAGCCACCAACCAGGACTTTATCCAGCG CCTCAGCACACTGATCCGCCAGGCCATTGAACGGCAACTCCCTGCCTGGATTGAGGCTGCCAACCAGCGC GAGGAAGCCCATGGTGAGCAGGGCGAGGAGGAAGAAGAGGAAGAGGAAGAGGAGGATGTTGCTGAGAACC GCTACTTTGAAATGGGACCCCCAGACGCAGAGGAAGAGGAGGGGAGTGGCCAGGGAGAAGAGGATGAGGA AGACGAGGACGAGGAGGCGGAGGAGGAGCGCCTGGCTCTGGAGTGGGCCCTGGGCGCCGATGAGGACTTC CTGCTGGAGCACATCCGCATCCTCAAGGTGCTCTGGTGCTTCCTGATCCACGTGCAAGGCAGCATCCGCC AGTTCGCTGCCTGCCTTGTGCTCACTGACTTCGGCATCGCAGTCTTTGAGATCCCACACCAAGAGTCAAG AGGCAGCAGCCAGCACATCCTCTCATCCCTGCGTTTTGTCTTCTGCTTCCCACATGGCGACCTCACGGAG TTTGGCTTCCTCATGCCCGAGCTGTGTCTGGTGCTCAAGGTGCGGCACAGTGAGAACACGCTCTTCATCA TCTCGGATGCTGCCAACTTACACGAGTTCCATGCTGATCTACGCTCCTGCTTTGCACCGCAGCACATGGC CATGCTGTGCAGCCCCATCCTCTACGGCAGCCACACCACCCTGCAGGAGTTCTTGCGCCAGCTGCTCACC TTCTACAAGGTGGCCGGGGGCTCTCAGGAGCGCAGCCAGGGCTGCTTCCCTGTCTACCTGGTCTACAGTG ACAAGCGCATGGTCCAGACCCCTGCCGGGGACTATTCAGGCAATATCGAATGGGCCAGCTGCACGCTGTG CTCGGCGGTGCGGCGCTCCTGCTGCGCGCCCTCGGAGGCCGTCAAGTCCGCTGCCATCCCCTACTGGCTG CTGCTCACATCCCAGCATCTCAACGTCATCAAGGCCGACTTCAACCCCATGCCCAATCGAGGCACCCACA ACTGCCGCAACCGCAACAGCTTCAAGCTTAGCCGCGTCCCGCTCTCTACCGTGCTGCTAGACCCCACTCG CAGCTGCACCCAGCCACGGGGTGCCTTCGCTGATGGCCATGTGCTCGAGCTGCTCGTGGGCTACCGCTTC GTTACCGCCATCTTTGTGCTGCCCCACGAGAAATTCCACTTCCTGCGAGTCTACAATCAGCTACGCGCCT CACTGCAGGACCTGAAGACTGTGGTCATCTCCAAGAATCCTTCAGCTAAGCCAAGAAATCAGCCTGCCAA GAGCAGGGCCAGTGCTGAGCAGCGGCTACAGGAGACCCCAGCAGACGCTCCGGCTCCAGCTGCAGTCCCA CCAACAGCTTCAGCTCCAGCCCCCGCAGAGGCCTTGGCTCCAGATCTGGCTCCTGTACAGGCCCCAGGAG AGGACCGAGGTCTAACTTCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCAGC CGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCG GCCCCAGCAGAGGCTCCAGCCGCAGCAGAGGCTCCGGCCGCAGCAGAGGCTCCAGCTGCAGCAGAGGCTC CAGCCGCAGCAGAGGCTCCAGCTTCAGCAGAGGCTCCAGCTCCAAACCAGGCTCCTGCTCCAGCAAGGGG TCCCGCTCCAGCAAGGGGTCCCGCTCCAGCAGGGGGTCCCGCTCCAGCAGAGGCCCTGGCACAAGCAGAA GTCCCTGCCCAGTACCCAAGTGAGCGCCTAATCCAGTCCACATCTGAAGAGAATCAGATCCCTTCTCACT TGCCAGTATGCCCATCACTCCAGCACATTGCCCGTCTTCGGGGGAGAGCCATCATTGACCTCTTCCACAA CAGCATTGCTGAGGTTGAAAATGAGGAGCTGAGGCACCTCCTGTGGTCATCAGTGGTGTTCTACCAGACC CCGGGGCTAGAAGTGACCGCCTGTGTGCTGCTCTCTAGCAAGGCTGTGTACTTCATACTGCATGATGGTC TCCGCCGCTACTTCTCTGAACCACTGCAGGATTTCTGGCACCAGAAAAACACCGACTATAACAACAGTCC TTTCCACGTCTCTCAGTGCTTTGTGTTGAAACTCAGTGACCTGCAGTCAGTCAACGTCGGCCTTTTCGAC CAGTACTTCCGGCTGACGGGCTCCTCCCCGACGCAGGTGGTCACGTGCTTGACTCGCGACAGCTACCTGA CGCACTGCTTCCTCCAGCATCTGATGCTTGTGCTGTCCTCCCTGGAGCGCACACCCTCGCCTGAGCCTGT TGACAAGGACTTCTACTCAGAATTTGGGGACAAGAATACAGGGAAAATGGAGAACTATGAGCTGATCCAT TCCAGCCGCGTCAAGTTCACCTACCCCAGTGAGGAAGAGGTTGGGGACCTGACCTATATTGTCGCACAGA AGATGGCTGATCCTGCAAAGAATCCAGCCCTCAGCATCTTACTGTACATCCAGGCCTTCCAGGTGGTCAC ACCACACCTTGGGCGGGGCAGGGGCCCACTGCGCCCTAAGACGCTGCTCCTGACCAGCGCCGAGATCTTC CTCCTGGATGAGGACTACATCCACTATCCATTGCCTGAATTTGCCAAAGAGCCACCGCAGAGGGACAGAT ACCGGCTAGACGATGGCCGCCGGGTCCGGGATTTGGACCGGGTGCTCATGGGCTACTATCCCTACCCACA GGCCCTCACTCTTGTTTTTGATGACACACAGGGTCACGACCTCATGGGGAGTGTCACCCTGGACCACTTT GGGGAGATGCCAGGTGGTCCTGGCAGGGTTGGCCAGGGCCGAGAGGTACAGTGGCAGGTGTTTGTCCCCA GTGCCGAGAGCCGAGAAAAGCTCATCTCACTGCTCGCACGACAGTGGGAAGCTCTTTGTGGCAGGGAGCT GCCTGTGGAGCTCACTGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022656
Insert Size	4782 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022656.2, NP_073147.2
RefSeq Size	5606 bp
RefSeq ORF	4782 bp
Locus ID	64652
UniProt ID	Q80TM9
Gene Summary	Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis (By similarity). Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3 (By similarity). When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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