Cltc (NM_001003908) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110065L21Rik; CHC; R74732
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224804 representing NM_001003908 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGATTCTGCCCATTCGCTTTCAGGAGCATCTCCAGCTCCAGAACCTGGGTATCAACCCAGCAA ACATTGGCTTCAGTACCTTGACTATGGAGTCAGACAAATTCATCTGCATTAGAGAAAAAGTGGGAGAGCA GGCCCAGGTGGTAATCATTGATATGAATGATCCAAGTAATCCAATTCGAAGACCAATCTCAGCAGACAGT GCCATCATGAATCCTGCCAGCAAAGTGATTGCACTGAAAGCTGGCAAAACTCTTCAGATATTTAACATTG AAATGAAAAGTAAAATGAAGGCCCATACCATGACTGATGATGTCACCTTCTGGAAATGGATCTCTTTGAA TACGGTTGCTCTTGTTACGGATAATGCAGTTTATCACTGGAGTATGGAAGGAGAGTCTCAGCCAGTGAAA ATGTTTGATCGTCATTCTAGCCTCGCAGGATGCCAGATCATCAATTACCGTACAGATGCAAAGCAGAAAT GGTTGCTTCTCACTGGCATATCTGCACAGCAAAATCGTGTGGTTGGAGCTATGCAGCTGTATTCTGTAGA TAGGAAAGTTTCTCAGCCTATTGAAGGGCATGCAGCTAGTTTTGCACAATTTAAGATGGAAGGAAATGCA GAAGAATCAACATTATTTTGTTTTGCTGTGCGGGGTCAGGCAGGAGGAAAGTTACATATCATTGAAGTTG GTACCCCACCCACAGGGAATCAACCCTTCCCAAAGAAGGCAGTGGATGTTTTCTTTCCTCCAGAAGCACA AAATGACTTTCCTGTTGCAATGCAGATCAGTGAAAAGCATGATGTGGTGTTCTTGATTACCAAGTATGGT TATATTCACCTCTATGATCTTGAGACTGGCACGTGCATCTACATGAACAGAATCAGCGGAGAAACCATCT TCGTCACTGCACCTCATGAAGCCACAGCTGGAATAATAGGAGTCAACAGAAAGGGACAGGTCCTGTCAGT TTGTGTGGAAGAGGAGAACATAATTCCTTATATCACCAATGTCCTACAAAATCCTGACTTGGCTCTGAGA ATGGCTGTGCGGAACAACTTAGCTGGTGCTGAAGAACTCTTTGCCCGGAAATTTAATGCTCTCTTCGCCC AGGGAAACTACTCAGAGGCTGCAAAGGTGGCTGCTAATGCTCCAAAGGGAATCCTTCGTACTCCAGATAC CATCCGTCGATTCCAGAGTGTCCCAGCTCAGCCAGGTCAAACGTCACCTCTACTTCAGTATTTCGGAATC CTTTTGGACCAGGGCCAGCTGAACAAATACGAGTCCTTAGAGTTATGTAGACCTGTACTTCAGCAAGGGC GGAAACAGCTTTTGGAGAAATGGCTGAAAGAAGATAAGCTGGAATGTTCTGAAGAACTGGGTGATCTCGT TAAATCTGTGGACCCAACATTAGCACTGAGTGTTTACCTCAGGGCCAATGTACCTAATAAAGTCATCCAG TGCTTTGCAGAGACAGGCCAAGTCCAGAAGATTGTTTTATATGCTAAAAAAGTTGGCTATACTCCAGACT GGATCTTTCTCCTGAGAAATGTCATGCGGATCAGTCCAGATCAAGGACAGCAGTTTGCTCAAATGTTAGT TCAGGATGAAGAGCCTCTTGCTGATATCACCCAGATTGTGGATGTCTTTATGGAATACAATCTAATCCAA CAATGTACTGCCTTCTTACTTGATGCTTTGAAGAATAATCGTCCCTCTGAAGGTCCTTTGCAGACGCGAT TGCTTGAGATGAACCTTATGCACGCACCTCAAGTTGCAGATGCCATTCTCGGGAATCAGATGTTCACCCA CTATGACCGGGCTCATATCGCCCAGCTGTGTGAGAAGGCGGGCCTGCTGCAGCGTGCATTAGAACACTTC ACCGATTTATATGACATAAAACGTGCAGTTGTTCATACCCATCTTCTTAACCCTGAGTGGTTAGTGAATT ACTTTGGGTCCTTATCAGTTGAAGATTCCCTAGAATGCCTCAGAGCCATGCTGTCAGCTAATATTCGTCA AAATCTACAGATCTGTGTTCAGGTGGCTTCTAAATATCACGAACAGCTGTCAACTCAGTCTCTGATTGAG CTCTTTGAATCATTCAAGAGTTTTGAAGGTCTCTTTTATTTTCTGGGGTCCATTGTTAACTTTAGTCAGG ACCCAGATGTGCACTTTAAATATATTCAGGCGGCTTGCAAGACTGGGCAAATCAAAGAAGTGGAAAGAAT CTGCAGAGAAAGCAACTGCTATGATCCCGAGCGAGTCAAGAATTTTCTCAAGGAGGCAAAACTAACAGAT CAGTTGCCACTTATCATTGTCTGTGATCGGTTTGACTTTGTCCATGACCTGGTGCTGTATTTATATAGAA ACAATCTTCAGAAGTATATAGAGATATATGTCCAGAAGGTAAATCCAAGTCGACTTCCTGTGGTTATTGG AGGATTGCTGGATGTTGACTGCTCTGAAGATGTCATTAAAAATTTGATTCTTGTTGTGAGAGGTCAATTC TCTACTGATGAGCTTGTTGCTGAAGTCGAGAAAAGAAACAGATTGAAGCTGCTTCTTCCTTGGCTTGAGG CCAGAATTCATGAGGGCTGTGAGGAGCCTGCTACTCACAATGCACTTGCCAAAATATACATTGACAGTAA CAACAACCCAGAGAGATTTCTTCGTGAAAACCCTTATTATGACAGTCGTGTTGTTGGGAAGTACTGTGAG AAGAGAGACCCCCATCTGGCTTGTGTTGCTTATGAACGTGGCCAGTGTGACCTGGAGCTCATCAATGTTT GCAATGAGAATTCCCTCTTCAAAAGCCTTTCTCGCTACTTAGTCCGTCGAAAGGATCCAGAATTGTGGGG TAGTGTGCTACTGGAAAGCAATCCATACAGAAGACCGTTAATTGACCAGGTTGTACAGACAGCCTTGTCT GAAACTCAGGACCCAGAAGAAGTGTCAGTAACTGTGAAGGCTTTTATGACAGCAGATCTTCCTAATGAGC TCATTGAACTGCTGGAGAAAATCGTTCTTGATAACTCCGTATTCAGTGAGCACAGGAATCTGCAAAATCT CCTCATCCTCACTGCAATTAAGGCTGACCGAACACGTGTTATGGAGTATATTAACCGCCTGGATAATTAT GATGCTCCAGATATTGCCAACATTGCCATCAGCAATGAGCTGTTTGAAGAGGCATTTGCTATTTTCCGGA AGTTTGATGTCAATACTTCAGCAGTGCAGGTGTTGATTGAACATATTGGAAACCTGGATCGTGCATATGA GTTTGCTGAACGCTGCAATGAGCCTGCTGTTTGGAGTCAACTTGCAAAAGCTCAGTTGCAGAAAGGAATG GTGAAGGAAGCCATTGATTCTTACATCAAAGCAGATGATCCTTCATCTTACATGGAAGTTGTCCAGGCTG CCAATGCCAGCGGAAACTGGGAAGAATTGGTGAAGTATTTGCAGATGGCACGCAAGAAGGCTCGAGAGTC CTATGTGGAGACAGAATTGATATTTGCACTGGCTAAAACAAACCGCCTTGCAGAATTAGAAGAGTTCATC AATGGGCCAAATAATGCTCATATTCAGCAAGTTGGTGACCGTTGTTATGATGAAAAAATGTATGATGCTG CTAAGTTGTTGTACAATAATGTTTCTAACTTTGGACGCTTGGCATCTACCCTTGTTCACCTTGGCGAGTA TCAGGCAGCAGTTGATGGTGCCAGAAAAGCTAATAGTACTCGAACATGGAAAGAGGTCTGCTTTGCCTGT GTAGATGGGAAAGAGTTCCGCCTTGCTCAGATGTGTGGACTCCATATTGTGGTGCATGCAGATGAATTAG AGGAGCTTATCAACTACTATCAGGATCGTGGATATTTTGAAGAACTCATCACCATGTTGGAGGCAGCCTT GGGACTGGAGCGAGCTCACATGGGAATGTTCACAGAGCTAGCTATTTTGTATTCTAAATTCAAGCCACAG AAAATGAGAGAACACCTGGAGTTGTTCTGGTCGAGAGTGAATATTCCTAAGGTGCTGAGAGCTGCAGAAC AGGCTCATCTCTGGGCAGAGCTGGTGTTTCTGTATGACAAGTACGAAGAGTATGACAATGCCATCATTAC CATGATGAATCACCCCACCGATGCATGGAAGGAAGGGCAGTTCAAAGACATCATCACCAAGGTGGCTAAT GTGGAACTGTACTACAAAGCAATCCAGTTCTACTTAGAATTCAAGCCTTTGTTGTTAAATGACTTGCTCA TGGTGCTGTCTCCACGGTTGGATCACACTCGTGCAGTCAATTATTTCAGCAAGGTAAAACAGCTACCACT GGTGAAACCATATTTACGCTCAGTGCAGAACCACAACAACAAATCTGTGAATGAATCACTGAACAACCTC TTTATTACTGAAGAAGATTACCAGGCTCTGCGAACATCAATAGATGCTTATGACAACTTTGACAACATCT CACTTGCTCAGCGTTTGGAAAAACACGAACTCATTGAATTCAGAAGAATTGCTGCTTATCTCTTCAAAGG CAACAATCGCTGGAAACAGAGTGTAGAGTTGTGCAAGAAAGACAGCCTTTACAAGGATGCAATGCAGTAT GCCTCTGAATCTAAAGACACAGAATTGGCTGAAGAGCTCCTACAGTGGTTTTTGCAGGAAGAAAAAAGAG AATGCTTTGGAGCTTGTCTCTTTACCTGTTATGATCTCTTAAGGCCAGATGTCGTCCTGGAAACCGCATG GAGACATAATATCATGGATTTCGCCATGCCCTATTTCATCCAGGTCATGAAGGAATATTTGACCAAGGTG GATAAATTAGATGCTTCGGAGTCGCTGAGAAAAGAAGAAGAACAAGCTACAGAGACACAGCCCATTGTTT ATGGTCAGCCCCAGCTGATGCTAACAGCAGGACCCAGTGTGGCAGTCCCTCCCCAGGCACCTTTTGGTTA TGGTTATACTGCACCACCGTATGGGCAGCCACAGCCTGGCTTTGGGTACAGCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001003908
Insert Size	5028 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001003908.1, NP_001003908.1
RefSeq Size	6178 bp
RefSeq ORF	5028 bp
Locus ID	67300
UniProt ID	Q68FD5
Gene Summary	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1.

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