Hc (NM_010406) Mouse Untagged Clone

CAT#: MC224814

Hc (untagged) - Mouse hemolytic complement (Hc), (10ug)



  "NM_010406" in other vectors (2)

CNY 26,980.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms C5; C5a; He
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224814 representing NM_010406
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTACCAGCCATGGGTCTTTGGGGAATACTTTGTCTTTTAATTTTCCTGGACAAAACTTGGGGAC
AGGAACAAACCTACGTCATTTCAGCACCCAAAATCCTCCGGGTCGGCTCGTCTGAAAATGTGGTAATTCA
AGTCCATGGCTACACTGAAGCATTTGATGCAACTCTTTCTCTAAAAAGCTATCCTGACAAAAAAGTCACC
TTCTCTTCAGGCTATGTTAATTTGTCCCCGGAAAACAAATTCCAAAACGCGGCACTGTTGACACTACAGC
CCAATCAAGTTCCTAGAGAAGAAAGCCCAGTCTCTCACGTGTATCTGGAAGTTGTGTCAAAACACTTTTC
AAAATCAAAGAAAATACCAATTACCTATAACAATGGAATTCTCTTCATCCATACAGACAAACCTGTTTAC
ACGCCGGACCAGTCAGTAAAGATCAGAGTCTATTCTCTGGGTGACGACTTGAAGCCAGCCAAACGGGAGA
CTGTCTTAACTTTCATAGACCCCGAAGGATCAGAAGTTGACATTGTAGAAGAAAATGATTACACCGGAAT
TATCTCTTTTCCTGACTTCAAGATTCCATCTAATCCCAAGTATGGTGTTTGGACAATTAAAGCTAACTAT
AAGAAGGATTTTACAACAACTGGAACTGCATACTTTGAAATTAAAGAATATGTCTTGCCACGATTCTCTG
TTTCAATAGAACTAGAAAGAACCTTCATTGGCTATAAAAACTTTAAGAACTTTGAAATCACTGTGAAAGC
AAGATATTTTTATAATAAAGTGGTACCTGATGCTGAAGTGTATGCCTTTTTTGGATTGAGAGAGGACATA
AAAGATGAGGAGAAGCAGATGATGCACAAAGCCACACAAGCCGCAAAGTTGGTTGACGGAGTTGCTCAGA
TCTCTTTTGATTCTGAAACAGCAGTTAAAGAGCTGTCCTACAACAGTCTAGAAGACTTAAACAACAAGTA
CCTTTATATTGCAGTAACAGTCACAGAATCTTCAGGTGGATTTTCAGAAGAGGCAGAAATCCCTGGAGTC
AAATATGTCCTCTCTCCCTACACACTGAATTTGGTCGCTACTCCTCTTTTCGTGAAGCCCGGGATTCCAT
TTTCCATCAAGGCACAGGTTAAAGATTCACTCGAGCAGGCGGTAGGAGGGGTCCCAGTAACTCTGATGGC
ACAAACAGTCGATGTGAATCAAGAGACATCTGACTTGGAAACAAAGAGGAGCATCACTCATGACACTGAT
GGAGTAGCTGTGTTTGTGCTGAACCTCCCATCAAATGTGACGGTGCTAAAGTTTGAGATCAGAACTGATG
ACCCAGAACTTCCCGAAGAAAATCAAGCCAGCAAAGAGTACGAAGCAGTTGCGTACTCGTCTCTCAGCCA
AAGTTACATTTACATCGCTTGGACTGAAAACTACAAGCCCATGCTTGTGGGAGAATACCTGAATATTATG
GTTACCCCCAAGAGCCCATATATCGACAAAATAACTCACTATAATTACTTGATTTTATCCAAAGGCAAAA
TTGTACAGTACGGCACAAGAGAGAAACTTTTCTCCTCAACTTATCAAAATATAAATATTCCAGTGACACA
GAACATGGTTCCTTCAGCACGACTCCTGGTCTATTACATAGTCACAGGGGAGCAAACAGCAGAATTAGTG
GCTGACGCAGTCTGGATAAATATTGAGGAGAAGTGTGGCAACCAGCTCCAGGTCCATCTGTCTCCAGATG
AATATGTGTATTCTCCAGGCCAAACTGTGTCCCTTGACATGGTGACTGAAGCAGACTCATGGGTAGCACT
ATCAGCAGTGGACAGAGCTGTGTATAAAGTCCAGGGAAACGCCAAAAGGGCCATGCAAAGAGTCTTTCAA
GCTTTGGATGAAAAGAGTGACCTGGGCTGTGGGGCAGGTGGTGGCCATGACAATGCAGATGTATTCCATC
TAGCTGGGCTCACCTTCCTCACCAACGCAAACGCAGATGACTCCCATTATCGTGATGACTCTTGTAAAGA
AATTCTCAGGTCAAAGAGAAACCTGCATCTCCTAAGGCAGAAAATAGAAGAACAAGCTGCTAAGTACAAA
CATAGTGTGCCAAAGAAATGCTGCTATGACGGAGCCCGAGTGAACTTCTACGAAACCTGTGAGGAGCGAG
TGGCCCGGGTTACCATAGGCCCTCTCTGCATCAGGGCCTTCAACGAGTGCTGTACTATTGCGAACAAGAT
CCGAAAAGAAAGCCCCCATAAACCTGTCCAACTGGGAAGGATCCACATTAAGACCCTGTTACCAGTGATG
AAGGCAGATATCCGAAGCTACTTTCCAGAGAGCTGGCTATGGGAAATTCATCGCGTTCCCAAAAGAAAAC
AGCTGCAGGTCACGCTGCCTGACTCACTAACGACTTGGGAAATTCAAGGCATTGGCATTTCAGACAATGG
TATATGTGTTGCTGATACACTCAAGGCAAAGGTGTTCAAAGAAGTCTTCCTGGAGATGAACATACCATAT
TCTGTTGTGCGAGGAGAACAGATCCAATTGAAAGGAACTGTTTACAACTATATGACCTCAGGGACAAAGT
TCTGTGTTAAAATGTCTGCTGTGGAGGGGATCTGCACTTCAGGAAGCTCAGCTGCTAGCCTTCACACCTC
CAGGCCCTCCAGATGTGTGTTCCAGAGGATAGAGGGCTCGTCCAGTCACTTGGTGACCTTCACCCTGCTT
CCTCTGGAAATTGGCCTTCACTCCATAAACTTCTCACTAGAGACCTCATTTGGGAAAGACATCTTAGTAA
AGACATTACGGGTAGTGCCAGAAGGAGTCAAGAGGGAAAGCTATGCCGGCGTGATTCTGGACCCTAAGGG
AATTCGTGGTATTGTTAACAGACGAAAGGAATTCCCATACAGGATCCCATTAGATTTGGTCCCCAAGACC
AAAGTTGAAAGGATTTTGAGTGTCAAAGGACTGCTTGTAGGGGAGTTCTTGTCCACGGTTCTGAGTAAGG
AAGGCATCAACATCCTAACCCACCTCCCCAAGGGCAGTGCAGAGGCAGAGCTCATGAGCATAGCTCCGGT
GTTCTATGTTTTCCACTACCTGGAAGCAGGAAACCATTGGAATATTTTCTATCCTGATACACTGAGTAAA
AGACAGAGCCTGGAGAAAAAAATAAAACAAGGGGTGGTGAGCGTCATGTCCTACAGAAACGCTGACTATT
CCTACAGCATGTGGAAGGGGGCGAGCGCTAGTACCTGGCTGACAGCTTTTGCTCTGAGAGTGCTTGGACA
GGTGGCCAAGTATGTAAAACAGGATGAAAACTCAATTTGTAACTCTTTGCTATGGCTGGTTGAGAAGTGT
CAGCTGGAAAACGGCTCTTTCAAGGAAAATTCCCAATATCTACCAATAAAATTACAGGGTACTTTGCCTG
CTGAAGCCCAAGAGAAAACTTTGTATCTTACAGCCTTTTCTGTGATTGGAATTAGAAAGGCAGTTGACAT
ATGCCCCACCATGAAAATCCACACAGCGCTAGATAAAGCCGACTCCTTCCTGCTTGAAAACACCCTGCCA
TCCAAGAGCACCTTCACACTGGCCATTGTAGCCTATGCTCTTTCCCTAGGAGACAGAACCCACCCGAGGT
TTCGTCTAATTGTGTCGGCCCTGAGGAAGGAAGCTTTTGTTAAAGGTGATCCGCCCATTTACCGTTACTG
GAGAGATACCCTCAAACGTCCAGACAGCTCTGTGCCCAGCAGCGGCACAGCAGGTATGGTTGAAACCACA
GCCTATGCTTTGCTCGCCAGCCTGAAACTGAAGGATATGAATTACGCCAACCCCATCATCAAGTGGCTAT
CTGAAGAGCAGAGGTATGGAGGCGGCTTTTATTCCACCCAGGATACGATTAATGCCATCGAGGGCCTGAC
AGAATATTCACTCCTGTTAAAACAAATTCATTTGGATATGGACATCAATGTCGCCTACAAACACGAAGGT
GACTTCCACAAGTATAAGGTGACAGAGAAGCATTTCCTGGGGAGGCCAGTGGAGGTATCTCTCAATGATG
ACCTTGTTGTCAGCACAGGCTACAGCAGTGGCTTGGCCACAGTATATGTAAAAACTGTGGTTCACAAAAT
TAGTGTCTCTGAGGAATTTTGCAGCTTTTACTTGAAAATTGATACCCAAGATATTGAAGCATCCAGCCAC
TTCAGGCTCAGTGACTCTGGATTCAAGCGCATAATAGCATGTGCCAGCTACAAGCCCAGCAAGGAGGAGT
CAACATCCGGGTCCTCCCATGCAGTAATGGATATATCACTGCCGACTGGAATCGGAGCAAACGAGGAAGA
TTTACGGGCTCTTGTGGAAGGAGTGGATCAACTACTAACTGATTACCAGATCAAAGATGGCCATGTCATT
CTGCAACTGAATTCGATCCCCTCCAGAGATTTCCTCTGTGTCCGGTTCCGGATATTTGAACTTTTCCAAG
TTGGGTTTCTGAATCCTGCTACCTTCACGGTGTACGAGTATCACAGACCAGATAAGCAGTGCACCATGAT
TTATAGCATTTCTGACACCAGGCTTCAGAAAGTCTGTGAAGGAGCAGCTTGCACATGTGTGGAAGCTGAC
TGTGCGCAACTGCAGGCAGAAGTAGACCTAGCCATCTCTGCAGACTCCAGAAAAGAGAAAGCCTGTAAAC
CAGAGACTGCATATGCTTATAAAGTCAGGATCACATCAGCCACTGAAGAAAATGTTTTTGTCAAGTACAC
TGCGACTCTTCTGGTCACTTACAAAACAGGGGAAGCTGCTGATGAGAATTCGGAGGTCACCTTCATTAAA
AAGATGAGCTGTACCAATGCCAACCTGGTGAAAGGGAAGCAGTATTTAATCATGGGCAAAGAGGTTCTGC
AGATCAAACACAATTTCAGTTTCAAGTATATATACCCTCTAGATTCCTCCACCTGGATTGAATATTGGCC
CACAGACACAACGTGTCCATCCTGTCAAGCATTTGTAGAGAATTTGAATAACTTTGCTGAAGACCTCTTT
TTAAACAGCTGTGAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010406
Insert Size 5058 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010406.2, NP_034536.2
RefSeq Size 5448 bp
RefSeq ORF 5058 bp
Locus ID 15139
UniProt ID P06684
Gene Summary This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model. [provided by RefSeq, Nov 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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