Hc (NM_010406) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C5; C5a; He
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224814 representing NM_010406 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTACCAGCCATGGGTCTTTGGGGAATACTTTGTCTTTTAATTTTCCTGGACAAAACTTGGGGAC AGGAACAAACCTACGTCATTTCAGCACCCAAAATCCTCCGGGTCGGCTCGTCTGAAAATGTGGTAATTCA AGTCCATGGCTACACTGAAGCATTTGATGCAACTCTTTCTCTAAAAAGCTATCCTGACAAAAAAGTCACC TTCTCTTCAGGCTATGTTAATTTGTCCCCGGAAAACAAATTCCAAAACGCGGCACTGTTGACACTACAGC CCAATCAAGTTCCTAGAGAAGAAAGCCCAGTCTCTCACGTGTATCTGGAAGTTGTGTCAAAACACTTTTC AAAATCAAAGAAAATACCAATTACCTATAACAATGGAATTCTCTTCATCCATACAGACAAACCTGTTTAC ACGCCGGACCAGTCAGTAAAGATCAGAGTCTATTCTCTGGGTGACGACTTGAAGCCAGCCAAACGGGAGA CTGTCTTAACTTTCATAGACCCCGAAGGATCAGAAGTTGACATTGTAGAAGAAAATGATTACACCGGAAT TATCTCTTTTCCTGACTTCAAGATTCCATCTAATCCCAAGTATGGTGTTTGGACAATTAAAGCTAACTAT AAGAAGGATTTTACAACAACTGGAACTGCATACTTTGAAATTAAAGAATATGTCTTGCCACGATTCTCTG TTTCAATAGAACTAGAAAGAACCTTCATTGGCTATAAAAACTTTAAGAACTTTGAAATCACTGTGAAAGC AAGATATTTTTATAATAAAGTGGTACCTGATGCTGAAGTGTATGCCTTTTTTGGATTGAGAGAGGACATA AAAGATGAGGAGAAGCAGATGATGCACAAAGCCACACAAGCCGCAAAGTTGGTTGACGGAGTTGCTCAGA TCTCTTTTGATTCTGAAACAGCAGTTAAAGAGCTGTCCTACAACAGTCTAGAAGACTTAAACAACAAGTA CCTTTATATTGCAGTAACAGTCACAGAATCTTCAGGTGGATTTTCAGAAGAGGCAGAAATCCCTGGAGTC AAATATGTCCTCTCTCCCTACACACTGAATTTGGTCGCTACTCCTCTTTTCGTGAAGCCCGGGATTCCAT TTTCCATCAAGGCACAGGTTAAAGATTCACTCGAGCAGGCGGTAGGAGGGGTCCCAGTAACTCTGATGGC ACAAACAGTCGATGTGAATCAAGAGACATCTGACTTGGAAACAAAGAGGAGCATCACTCATGACACTGAT GGAGTAGCTGTGTTTGTGCTGAACCTCCCATCAAATGTGACGGTGCTAAAGTTTGAGATCAGAACTGATG ACCCAGAACTTCCCGAAGAAAATCAAGCCAGCAAAGAGTACGAAGCAGTTGCGTACTCGTCTCTCAGCCA AAGTTACATTTACATCGCTTGGACTGAAAACTACAAGCCCATGCTTGTGGGAGAATACCTGAATATTATG GTTACCCCCAAGAGCCCATATATCGACAAAATAACTCACTATAATTACTTGATTTTATCCAAAGGCAAAA TTGTACAGTACGGCACAAGAGAGAAACTTTTCTCCTCAACTTATCAAAATATAAATATTCCAGTGACACA GAACATGGTTCCTTCAGCACGACTCCTGGTCTATTACATAGTCACAGGGGAGCAAACAGCAGAATTAGTG GCTGACGCAGTCTGGATAAATATTGAGGAGAAGTGTGGCAACCAGCTCCAGGTCCATCTGTCTCCAGATG AATATGTGTATTCTCCAGGCCAAACTGTGTCCCTTGACATGGTGACTGAAGCAGACTCATGGGTAGCACT ATCAGCAGTGGACAGAGCTGTGTATAAAGTCCAGGGAAACGCCAAAAGGGCCATGCAAAGAGTCTTTCAA GCTTTGGATGAAAAGAGTGACCTGGGCTGTGGGGCAGGTGGTGGCCATGACAATGCAGATGTATTCCATC TAGCTGGGCTCACCTTCCTCACCAACGCAAACGCAGATGACTCCCATTATCGTGATGACTCTTGTAAAGA AATTCTCAGGTCAAAGAGAAACCTGCATCTCCTAAGGCAGAAAATAGAAGAACAAGCTGCTAAGTACAAA CATAGTGTGCCAAAGAAATGCTGCTATGACGGAGCCCGAGTGAACTTCTACGAAACCTGTGAGGAGCGAG TGGCCCGGGTTACCATAGGCCCTCTCTGCATCAGGGCCTTCAACGAGTGCTGTACTATTGCGAACAAGAT CCGAAAAGAAAGCCCCCATAAACCTGTCCAACTGGGAAGGATCCACATTAAGACCCTGTTACCAGTGATG AAGGCAGATATCCGAAGCTACTTTCCAGAGAGCTGGCTATGGGAAATTCATCGCGTTCCCAAAAGAAAAC AGCTGCAGGTCACGCTGCCTGACTCACTAACGACTTGGGAAATTCAAGGCATTGGCATTTCAGACAATGG TATATGTGTTGCTGATACACTCAAGGCAAAGGTGTTCAAAGAAGTCTTCCTGGAGATGAACATACCATAT TCTGTTGTGCGAGGAGAACAGATCCAATTGAAAGGAACTGTTTACAACTATATGACCTCAGGGACAAAGT TCTGTGTTAAAATGTCTGCTGTGGAGGGGATCTGCACTTCAGGAAGCTCAGCTGCTAGCCTTCACACCTC CAGGCCCTCCAGATGTGTGTTCCAGAGGATAGAGGGCTCGTCCAGTCACTTGGTGACCTTCACCCTGCTT CCTCTGGAAATTGGCCTTCACTCCATAAACTTCTCACTAGAGACCTCATTTGGGAAAGACATCTTAGTAA AGACATTACGGGTAGTGCCAGAAGGAGTCAAGAGGGAAAGCTATGCCGGCGTGATTCTGGACCCTAAGGG AATTCGTGGTATTGTTAACAGACGAAAGGAATTCCCATACAGGATCCCATTAGATTTGGTCCCCAAGACC AAAGTTGAAAGGATTTTGAGTGTCAAAGGACTGCTTGTAGGGGAGTTCTTGTCCACGGTTCTGAGTAAGG AAGGCATCAACATCCTAACCCACCTCCCCAAGGGCAGTGCAGAGGCAGAGCTCATGAGCATAGCTCCGGT GTTCTATGTTTTCCACTACCTGGAAGCAGGAAACCATTGGAATATTTTCTATCCTGATACACTGAGTAAA AGACAGAGCCTGGAGAAAAAAATAAAACAAGGGGTGGTGAGCGTCATGTCCTACAGAAACGCTGACTATT CCTACAGCATGTGGAAGGGGGCGAGCGCTAGTACCTGGCTGACAGCTTTTGCTCTGAGAGTGCTTGGACA GGTGGCCAAGTATGTAAAACAGGATGAAAACTCAATTTGTAACTCTTTGCTATGGCTGGTTGAGAAGTGT CAGCTGGAAAACGGCTCTTTCAAGGAAAATTCCCAATATCTACCAATAAAATTACAGGGTACTTTGCCTG CTGAAGCCCAAGAGAAAACTTTGTATCTTACAGCCTTTTCTGTGATTGGAATTAGAAAGGCAGTTGACAT ATGCCCCACCATGAAAATCCACACAGCGCTAGATAAAGCCGACTCCTTCCTGCTTGAAAACACCCTGCCA TCCAAGAGCACCTTCACACTGGCCATTGTAGCCTATGCTCTTTCCCTAGGAGACAGAACCCACCCGAGGT TTCGTCTAATTGTGTCGGCCCTGAGGAAGGAAGCTTTTGTTAAAGGTGATCCGCCCATTTACCGTTACTG GAGAGATACCCTCAAACGTCCAGACAGCTCTGTGCCCAGCAGCGGCACAGCAGGTATGGTTGAAACCACA GCCTATGCTTTGCTCGCCAGCCTGAAACTGAAGGATATGAATTACGCCAACCCCATCATCAAGTGGCTAT CTGAAGAGCAGAGGTATGGAGGCGGCTTTTATTCCACCCAGGATACGATTAATGCCATCGAGGGCCTGAC AGAATATTCACTCCTGTTAAAACAAATTCATTTGGATATGGACATCAATGTCGCCTACAAACACGAAGGT GACTTCCACAAGTATAAGGTGACAGAGAAGCATTTCCTGGGGAGGCCAGTGGAGGTATCTCTCAATGATG ACCTTGTTGTCAGCACAGGCTACAGCAGTGGCTTGGCCACAGTATATGTAAAAACTGTGGTTCACAAAAT TAGTGTCTCTGAGGAATTTTGCAGCTTTTACTTGAAAATTGATACCCAAGATATTGAAGCATCCAGCCAC TTCAGGCTCAGTGACTCTGGATTCAAGCGCATAATAGCATGTGCCAGCTACAAGCCCAGCAAGGAGGAGT CAACATCCGGGTCCTCCCATGCAGTAATGGATATATCACTGCCGACTGGAATCGGAGCAAACGAGGAAGA TTTACGGGCTCTTGTGGAAGGAGTGGATCAACTACTAACTGATTACCAGATCAAAGATGGCCATGTCATT CTGCAACTGAATTCGATCCCCTCCAGAGATTTCCTCTGTGTCCGGTTCCGGATATTTGAACTTTTCCAAG TTGGGTTTCTGAATCCTGCTACCTTCACGGTGTACGAGTATCACAGACCAGATAAGCAGTGCACCATGAT TTATAGCATTTCTGACACCAGGCTTCAGAAAGTCTGTGAAGGAGCAGCTTGCACATGTGTGGAAGCTGAC TGTGCGCAACTGCAGGCAGAAGTAGACCTAGCCATCTCTGCAGACTCCAGAAAAGAGAAAGCCTGTAAAC CAGAGACTGCATATGCTTATAAAGTCAGGATCACATCAGCCACTGAAGAAAATGTTTTTGTCAAGTACAC TGCGACTCTTCTGGTCACTTACAAAACAGGGGAAGCTGCTGATGAGAATTCGGAGGTCACCTTCATTAAA AAGATGAGCTGTACCAATGCCAACCTGGTGAAAGGGAAGCAGTATTTAATCATGGGCAAAGAGGTTCTGC AGATCAAACACAATTTCAGTTTCAAGTATATATACCCTCTAGATTCCTCCACCTGGATTGAATATTGGCC CACAGACACAACGTGTCCATCCTGTCAAGCATTTGTAGAGAATTTGAATAACTTTGCTGAAGACCTCTTT TTAAACAGCTGTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010406
Insert Size	5058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010406.2, NP_034536.2
RefSeq Size	5448 bp
RefSeq ORF	5058 bp
Locus ID	15139
UniProt ID	P06684
Gene Summary	This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model. [provided by RefSeq, Nov 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.