Hc (NM_010406) Mouse Tagged ORF Clone
CAT#: MR225274
- TrueORF®
Hc (Myc-DDK-tagged) - Mouse hemolytic complement (Hc)
ORF Plasmid: tGFP
"NM_010406" in other vectors (2)
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CNY 11,296.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C5; C5a; He |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225274 representing NM_010406
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCTTTGGGGAATACTTTGTCTTTTAATTTTCCTGGACAAAACTTGGGGACAGGAACAAACCTACG TCATTTCAGCACCCAAAATCCTCCGGGTCGGCTCGTCTGAAAATGTGGTAATTCAAGTCCATGGCTACAC TGAAGCATTTGATGCAACTCTTTCTCTAAAAAGCTATCCTGACAAAAAAGTCACCTTCTCTTCAGGCTAT GTTAATTTGTCCCCGGAAAACAAATTCCAAAACGCGGCACTGTTGACACTACAGCCCAATCAAGTTCCTA GAGAAGAAAGCCCAGTCTCTCACGTGTATCTGGAAGTTGTGTCAAAACACTTTTCAAAATCAAAGAAAAT ACCAATTACCTATAACAATGGAATTCTCTTCATCCATACAGACAAACCTGTTTACACGCCGGACCAGTCA GTAAAGATCAGAGTCTATTCTCTGGGTGACGACTTGAAGCCAGCCAAACGGGAGACTGTCTTAACTTTCA TAGACCCCGAAGGATCAGAAGTTGACATTGTAGAAGAAAATGATTACACCGGAATTATCTCTTTTCCTGA CTTCAAGATTCCATCTAATCCCAAGTATGGTGTTTGGACAATTAAAGCTAACTATAAGAAGGATTTTACA ACAACTGGAACTGCATACTTTGAAATTAAAGAATATGTCTTGCCACGATTCTCTGTTTCAATAGAACTAG AAAGAACCTTCATTGGCTATAAAAACTTTAAGAACTTTGAAATCACTGTGAAAGCAAGATATTTTTATAA TAAAGTGGTACCTGATGCTGAAGTGTATGCCTTTTTTGGATTGAGAGAGGACATAAAAGATGAGGAGAAG CAGATGATGCACAAAGCCACACAAGCCGCAAAGTTGGTTGACGGAGTTGCTCAGATCTCTTTTGATTCTG AAACAGCAGTTAAAGAGCTGTCCTACAACAGTCTAGAAGACTTAAACAACAAGTACCTTTATATTGCAGT AACAGTCACAGAATCTTCAGGTGGATTTTCAGAAGAGGCAGAAATCCCTGGAGTCAAATATGTCCTCTCT CCCTACACACTGAATTTGGTCGCTACTCCTCTTTTCGTGAAGCCCGGGATTCCATTTTCCATCAAGGCAC AGGTTAAAGATTCACTCGAGCAGGCGGTAGGAGGGGTCCCAGTAACTCTGATGGCACAAACAGTCGATGT GAATCAAGAGACATCTGACTTGGAAACAAAGAGGAGCATCACTCATGACACTGATGGAGTAGCTGTGTTT GTGCTGAACCTCCCATCAAATGTGACGGTGCTAAAGTTTGAGATCAGAACTGATGACCCAGAACTTCCCG AAGAAAATCAAGCCAGCAAAGAGTACGAAGCAGTTGCGTACTCGTCTCTCAGCCAAAGTTACATTTACAT CGCTTGGACTGAAAACTACAAGCCCATGCTTGTGGGAGAATACCTGAATATTATGGTTACCCCCAAGAGC CCATATATCGACAAAATAACTCACTATAATTACTTGATTTTATCCAAAGGCAAAATTGTACAGTACGGCA CAAGAGAGAAACTTTTCTCCTCAACTTATCAAAATATAAATATTCCAGTGACACAGAACATGGTTCCTTC AGCACGACTCCTGGTCTATTACATAGTCACAGGGGAGCAAACAGCAGAATTAGTGGCTGACGCAGTCTGG ATAAATATTGAGGAGAAGTGTGGCAACCAGCTCCAGGTCCATCTGTCTCCAGATGAATATGTGTATTCTC CAGGCCAAACTGTGTCCCTTGACATGGTGACTGAAGCAGACTCATGGGTAGCACTATCAGCAGTGGACAG AGCTGTGTATAAAGTCCAGGGAAACGCCAAAAGGGCCATGCAAAGAGTCTTTCAAGCTTTGGATGAAAAG AGTGACCTGGGCTGTGGGGCAGGTGGTGGCCATGACAATGCAGATGTATTCCATCTAGCTGGGCTCACCT TCCTCACCAACGCAAACGCAGATGACTCCCATTATCGTGATGACTCTTGTAAAGAAATTCTCAGGTCAAA GAGAAACCTGCATCTCCTAAGGCAGAAAATAGAAGAACAAGCTGCTAAGTACAAACATAGTGTGCCAAAG AAATGCTGCTATGACGGAGCCCGAGTGAACTTCTACGAAACCTGTGAGGAGCGAGTGGCCCGGGTTACCA TAGGCCCTCTCTGCATCAGGGCCTTCAACGAGTGCTGTACTATTGCGAACAAGATCCGAAAAGAAAGCCC CCATAAACCTGTCCAACTGGGAAGGATCCACATTAAGACCCTGTTACCAGTGATGAAGGCAGATATCCGA AGCTACTTTCCAGAGAGCTGGCTATGGGAAATTCATCGCGTTCCCAAAAGAAAACAGCTGCAGGTCACGC TGCCTGACTCACTAACGACTTGGGAAATTCAAGGCATTGGCATTTCAGACAATGGTATATGTGTTGCTGA TACACTCAAGGCAAAGGTGTTCAAAGAAGTCTTCCTGGAGATGAACATACCATATTCTGTTGTGCGAGGA GAACAGATCCAATTGAAAGGAACTGTTTACAACTATATGACCTCAGGGACAAAGTTCTGTGTTAAAATGT CTGCTGTGGAGGGGATCTGCACTTCAGGAAGCTCAGCTGCTAGCCTTCACACCTCCAGGCCCTCCAGATG TGTGTTCCAGAGGATAGAGGGCTCGTCCAGTCACTTGGTGACCTTCACCCTGCTTCCTCTGGAAATTGGC CTTCACTCCATAAACTTCTCACTAGAGACCTCATTTGGGAAAGACATCTTAGTAAAGACATTACGGGTAG TGCCAGAAGGAGTCAAGAGGGAAAGCTATGCCGGCGTGATTCTGGACCCTAAGGGAATTCGTGGTATTGT TAACAGACGAAAGGAATTCCCATACAGGATCCCATTAGATTTGGTCCCCAAGACCAAAGTTGAAAGGATT TTGAGTGTCAAAGGACTGCTTGTAGGGGAGTTCTTGTCCACGGTTCTGAGTAAGGAAGGCATCAACATCC TAACCCACCTCCCCAAGGGCAGTGCAGAGGCAGAGCTCATGAGCATAGCTCCGGTGTTCTATGTTTTCCA CTACCTGGAAGCAGGAAACCATTGGAATATTTTCTATCCTGATACACTGAGTAAAAGACAGAGCCTGGAG AAAAAAATAAAACAAGGGGTGGTGAGCGTCATGTCCTACAGAAACGCTGACTATTCCTACAGCATGTGGA AGGGGGCGAGCGCTAGTACCTGGCTGACAGCTTTTGCTCTGAGAGTGCTTGGACAGGTGGCCAAGTATGT AAAACAGGATGAAAACTCAATTTGTAACTCTTTGCTATGGCTGGTTGAGAAGTGTCAGCTGGAAAACGGC TCTTTCAAGGAAAATTCCCAATATCTACCAATAAAATTACAGGGTACTTTGCCTGCTGAAGCCCAAGAGA AAACTTTGTATCTTACAGCCTTTTCTGTGATTGGAATTAGAAAGGCAGTTGACATATGCCCCACCATGAA AATCCACACAGCGCTAGATAAAGCCGACTCCTTCCTGCTTGAAAACACCCTGCCATCCAAGAGCACCTTC ACACTGGCCATTGTAGCCTATGCTCTTTCCCTAGGAGACAGAACCCACCCGAGGTTTCGTCTAATTGTGT CGGCCCTGAGGAAGGAAGCTTTTGTTAAAGGTGATCCGCCCATTTACCGTTACTGGAGAGATACCCTCAA ACGTCCAGACAGCTCTGTGCCCAGCAGCGGCACAGCAGGTATGGTTGAAACCACAGCCTATGCTTTGCTC GCCAGCCTGAAACTGAAGGATATGAATTACGCCAACCCCATCATCAAGTGGCTATCTGAAGAGCAGAGGT ATGGAGGCGGCTTTTATTCCACCCAGGATACGATTAATGCCATCGAGGGCCTGACAGAATATTCACTCCT GTTAAAACAAATTCATTTGGATATGGACATCAATGTCGCCTACAAACACGAAGGTGACTTCCACAAGTAT AAGGTGACAGAGAAGCATTTCCTGGGGAGGCCAGTGGAGGTATCTCTCAATGATGACCTTGTTGTCAGCA CAGGCTACAGCAGTGGCTTGGCCACAGTATATGTAAAAACTGTGGTTCACAAAATTAGTGTCTCTGAGGA ATTTTGCAGCTTTTACTTGAAAATTGATACCCAAGATATTGAAGCATCCAGCCACTTCAGGCTCAGTGAC TCTGGATTCAAGCGCATAATAGCATGTGCCAGCTACAAGCCCAGCAAGGAGGAGTCAACATCCGGGTCCT CCCATGCAGTAATGGATATATCACTGCCGACTGGAATCGGAGCAAACGAGGAAGATTTACGGGCTCTTGT GGAAGGAGTGGATCAACTACTAACTGATTACCAGATCAAAGATGGCCATGTCATTCTGCAACTGAATTCG ATCCCCTCCAGAGATTTCCTCTGTGTCCGGTTCCGGATATTTGAACTTTTCCAAGTTGGGTTTCTGAATC CTGCTACCTTCACGGTGTACGAGTATCACAGACCAGATAAGCAGTGCACCATGATTTATAGCATTTCTGA CACCAGGCTTCAGAAAGTCTGTGAAGGAGCAGCTTGCACATGTGTGGAAGCTGACTGTGCGCAACTGCAG GCAGAAGTAGACCTAGCCATCTCTGCAGACTCCAGAAAAGAGAAAGCCTGTAAACCAGAGACTGCATATG CTTATAAAGTCAGGATCACATCAGCCACTGAAGAAAATGTTTTTGTCAAGTACACTGCGACTCTTCTGGT CACTTACAAAACAGGGGAAGCTGCTGATGAGAATTCGGAGGTCACCTTCATTAAAAAGATGAGCTGTACC AATGCCAACCTGGTGAAAGGGAAGCAGTATTTAATCATGGGCAAAGAGGTTCTGCAGATCAAACACAATT TCAGTTTCAAGTATATATACCCTCTAGATTCCTCCACCTGGATTGAATATTGGCCCACAGACACAACGTG TCCATCCTGTCAAGCATTTGTAGAGAATTTGAATAACTTTGCTGAAGACCTCTTTTTAAACAGCTGTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225274 representing NM_010406
Red=Cloning site Green=Tags(s) MGLWGILCLLIFLDKTWGQEQTYVISAPKILRVGSSENVVIQVHGYTEAFDATLSLKSYPDKKVTFSSGY VNLSPENKFQNAALLTLQPNQVPREESPVSHVYLEVVSKHFSKSKKIPITYNNGILFIHTDKPVYTPDQS VKIRVYSLGDDLKPAKRETVLTFIDPEGSEVDIVEENDYTGIISFPDFKIPSNPKYGVWTIKANYKKDFT TTGTAYFEIKEYVLPRFSVSIELERTFIGYKNFKNFEITVKARYFYNKVVPDAEVYAFFGLREDIKDEEK QMMHKATQAAKLVDGVAQISFDSETAVKELSYNSLEDLNNKYLYIAVTVTESSGGFSEEAEIPGVKYVLS PYTLNLVATPLFVKPGIPFSIKAQVKDSLEQAVGGVPVTLMAQTVDVNQETSDLETKRSITHDTDGVAVF VLNLPSNVTVLKFEIRTDDPELPEENQASKEYEAVAYSSLSQSYIYIAWTENYKPMLVGEYLNIMVTPKS PYIDKITHYNYLILSKGKIVQYGTREKLFSSTYQNINIPVTQNMVPSARLLVYYIVTGEQTAELVADAVW INIEEKCGNQLQVHLSPDEYVYSPGQTVSLDMVTEADSWVALSAVDRAVYKVQGNAKRAMQRVFQALDEK SDLGCGAGGGHDNADVFHLAGLTFLTNANADDSHYRDDSCKEILRSKRNLHLLRQKIEEQAAKYKHSVPK KCCYDGARVNFYETCEERVARVTIGPLCIRAFNECCTIANKIRKESPHKPVQLGRIHIKTLLPVMKADIR SYFPESWLWEIHRVPKRKQLQVTLPDSLTTWEIQGIGISDNGICVADTLKAKVFKEVFLEMNIPYSVVRG EQIQLKGTVYNYMTSGTKFCVKMSAVEGICTSGSSAASLHTSRPSRCVFQRIEGSSSHLVTFTLLPLEIG LHSINFSLETSFGKDILVKTLRVVPEGVKRESYAGVILDPKGIRGIVNRRKEFPYRIPLDLVPKTKVERI LSVKGLLVGEFLSTVLSKEGINILTHLPKGSAEAELMSIAPVFYVFHYLEAGNHWNIFYPDTLSKRQSLE KKIKQGVVSVMSYRNADYSYSMWKGASASTWLTAFALRVLGQVAKYVKQDENSICNSLLWLVEKCQLENG SFKENSQYLPIKLQGTLPAEAQEKTLYLTAFSVIGIRKAVDICPTMKIHTALDKADSFLLENTLPSKSTF TLAIVAYALSLGDRTHPRFRLIVSALRKEAFVKGDPPIYRYWRDTLKRPDSSVPSSGTAGMVETTAYALL ASLKLKDMNYANPIIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLLKQIHLDMDINVAYKHEGDFHKY KVTEKHFLGRPVEVSLNDDLVVSTGYSSGLATVYVKTVVHKISVSEEFCSFYLKIDTQDIEASSHFRLSD SGFKRIIACASYKPSKEESTSGSSHAVMDISLPTGIGANEEDLRALVEGVDQLLTDYQIKDGHVILQLNS IPSRDFLCVRFRIFELFQVGFLNPATFTVYEYHRPDKQCTMIYSISDTRLQKVCEGAACTCVEADCAQLQ AEVDLAISADSRKEKACKPETAYAYKVRITSATEENVFVKYTATLLVTYKTGEAADENSEVTFIKKMSCT NANLVKGKQYLIMGKEVLQIKHNFSFKYIYPLDSSTWIEYWPTDTTCPSCQAFVENLNNFAEDLFLNSCE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010406 |
ORF Size | 5040 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010406.3 |
RefSeq Size | 5448 bp |
RefSeq ORF | 5058 bp |
Locus ID | 15139 |
UniProt ID | P06684 |
MW | 189.3 kDa |
Gene Summary | This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model. [provided by RefSeq, Nov 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Design and preclinical characterization of ALXN1210: A novel anti-C5 antibody with extended duration of action
,Sheridan, D;Yu, ZX;Zhang, Y;Patel, R;Sun, F;Lasaro, MA;Bouchard, K;Andrien, B;Marozsan, A;Wang, Y;Tamburini, P;,
PLoS ONE
,PubMed ID 29649283
[HC]
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