Ptpn23 (NM_001081043) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI462446; PTP-TD14
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224834 representing NM_001081043 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGTGCCCCGGATGCCCATGATCTGGCTGGACCTGAAGGAGGCCGGTGACTTTCACTTCCAGT CTGCGGTGAAGAAGTTTGTCCTGAAGAATTATGGAGAGAACCCAGAAGCCTACAACGAGGAACTAAAGAA GCTGGAATTACTCAGACAGAATGCTATTCGCGTTGCCCGGGACTTTGAGGGCTGCAGTGTTCTTCGAAAG TACCTTGGCCAGCTACACTACCTACAGAGTCGGGTGCCCATGGGCTCAGGCCAGGAGGCTGCTGTAGCTG TCACCTGGACTGAGATCTTCTCGGGCAAGTCTGTGTCCCATGAAGACATCAAGTACGAGCAGGCCTGTAT TCTCTACAACCTCGGTGCACTGCACTCCATGCTGGGGGCTATGGACAAGCGGGTGTCTGAGGAGGGCATG AAAGTCTCCTGCACCCACTTCCAGTGTGCAGCAGGCGCCTTTGCCTACCTGCGTGAACACTTCCCACAGG CCTTCAGTGTGGACATGAGTCGCCAGATCCTTACGCTCAATGTCAACCTCATGCTGGGCCAGGCTCAGGA GTGCCTGTTGGAGAAGTCCATGTTGGACAACCGGAAGAGCTTTCTAGTGGCCCGCATCAGCGCACAGGTG GTAGATTACTATAAGGAGGCATGCCGGGCCTTGGAAAACCCTGATACTGCCTCCCTGCTGGGCCGTATCC AGAAAGACTGGAAGAAACTTGTCCAAATGAAGATCTACTACTTTGCGGCTGTGGCTCATCTACACATGGG GAAGCAGGCTGAGGAGCAACAGAAATTTGGGGAACGGGTGGCCTACTTCCAGAGTGCCCTGGACAAGCTT AATGAAGCCATCAAGTTGGCCAAGGGCCAGCCCGACACTGTGCAAGATGCACTTCGCTTTGCTATGGATG TCATCGGGGGCAAATATAATTCTGCCAAGAAGGACAATGACTTTATCTACCATGAGGCTGTGCCAGCACT GGATACACTCCAGCCTGTGAAAGGAGCTCCCTTGGTGAAGCCCTTGCCAGTAAACCCCACAGACCCAGCT GTTACAGGTCCTGACATCTTTGCCAAGTTGGTGCCCATGGCTGCCCATGAGGCCTCATCACTGTACAGTG AGGAGAAGGCCAAACTGCTTCGGGAGATGCTGGCCAAAATTGAAGATAAGAATGAGGTCCTAGATCAGTT CATGGACTCCATGCAGCTGGACCCTGAGACAGTGGACAACTTGGATGCCTACAACCATATCCCCCCCCAG CTCATGGAGAAGTGTGCGGCACTCAGCGTCCGGCCTGACACTGTCAAGAACCTTGTCCAGTCTATGCAGG TGCTGTCAGGGGTGTTCACGGATGTGGAGGCCTCCCTGAAGGACATCAGGGATCTACTGGAGGAAGATGA GCTGCAAGAGCAGAAATTGCAGGAGACCCTGGGCCAGGCCGGGGCTGGCCCTGGCCCCTCTGTTGCCAAG GCTGAGCTTGCAGAGGTGAGGCGAGAATGGGCCAAGTACATGGAAGTGCATGAAAAGGCTTCCTTCACCA ACAGTGAGCTGCATCGTGCTATGAACCTGCATGTTGGTAACTTGCGCCTGCTCAGTGGACCACTGGACCA GGTGCGGGCTGCCCTACCCACACCAGCCCTCACCCCAGAGGACAAAGCCGTGCTGCAAAACCTAAAGCGC ATCCTCGCCAAGGTGCAGGAGATGCGGGACCAGCGTGTGTCCTTGGAACAGCAGCTCCGAGAGCTGATCC AGAAGGATGACATCACTGCCTCTCTGGTCACCACTGACCACTCAGAGATGAAGAAACTGTTTGAGGAGCA GCTGAAGAAGTATGACCAGCTGAAGGTCTACCTGGAGCAGAACCTGGCCGCCCAGGACAATGTCCTCCGT GCCCTGACTGAGGCCAACGTGCAGTATGCAGCCGTGCGGCGGGTGCTTAGTGAGCTGGACCAAAAGTGGA ACTCCACACTACAGACCCTGGTAGCATCCTATGAGGCCTATGAGGACCTGATGAAGAAGTCCCAAGAAGG CAAGGACTTTTATGCAGACTTGGAAAGCAAGGTAGCTACCCTTCTGGAACGGGCACAATCCATCTGTCGT GCCCAAGAGGCCGCTCGCCAGCAGCTCCTGGACAGAGAGCTGAAGAAGAAGGCACCACCACCTCGGCCTA CAGCCCCAAAGCCATTGCTGTCCCGCCGTGAGGAGGGGGAGGCAGTGGAGGCTGGAGACACACCTGAGGA GCTGCGCAGCTTGCCCCCTGACATGATGGTTGGCCCACGGCTACCTGACCCATTCCTAGGAACTACTGCC CCTCTCCACTTTTCTCCTGGCCCCTTCCCTAGTTCCACAGGCCCAGCAACCCACTATCTCTCAGGACCCT TACCCCCTGGAACTTATTCAGGCCCCACTCAACTGATGCAGCCTAGGGCTGCAGTTCCCATGGCACCTGC AACGGTCCTCTACCCAGCCCCTGCCTATACATCGGAGTTGGGCCTCGTACCCAGATCCTCTCCCCAGCAT GGAATTGTAAGCAGTCCCTATGCAGGGGTAGGGCCACCCCAACCAGTTGTAGGTCTTCCCTCTGCTCCAC CTCCCCAGCTCTCAGGACCCGAGTTAGCCATGACAGTTCGGCCAGCTACCACCACAGTAGATAGTGTCCA AGCCCCTATCTCTAGCCACACGGCACCACGGCCAAACCCTACCCCTGCTCTTCCCCAGCCCTGCTTTCCA GTGCCTCAGCCTGTACCTCAATCTGTTCCCCAACCACAGCCTCTGCCTGTACCCTATACTTATTCTATAG GGACCAAGCAGCCTCTGCCTGCACCCTATACTTACTCTATAGGGACCAAGCAGCACCTCACTGGCCCTCT ACCACAGCATCAGTTTCCTCCTGGGATCCCCACAGGCTTTCCAGTCCCCAGGACTGGGCCCCAGGCCCAG GCCCAGCCCCAACCCCAGCCCCAGCCCCAGCCTCAGCCTCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCC AGTCCCAGTCCCAGCCCCAGCCCCAGCCCCAGCCTCAGCCCCAACGGCCAGCATTTGGGCCACAACCCAC ACAACAGCCCCTTCCATTCCAGCATCCACATCTCTTCCCATCTCAGGCCCCAGGGATTCTTCCACCACCA CCACCAACACCCTACCATTTTACTCCTCAGCCTGGTGTCTTGGGACAACCACCACCCACCCTACACACCC AGCTCTATCCAGGCCCATCTCAAGACCCTCTTCCCCCACATTCAGGGGCTCTGCCTTTCCCCAGCCCTGG GCCCCCTCATCCTCACCCCACCCTGGCATATGGCCCTGCCCCTTCCCCTAGGCCCCTTGGTCCTCAGGCA ACCCCTGTCTCCATTCGAGGCCCCCCACCAGCCAGTCAGCCCACCCCTAGTCCACACTTGGTGCCTTCAC CTGCCCCATCACCAGGGCCTGGCCCAGTACCTTCAAGACCTCCAACAGCCGAGCCACCACCTTGTTTGCG CCGAGGTGCTGCAGCTGCAGACCTTCTGTCTTCTAGCCCTGAGAGCCAGCATGGAGGTACCCAGCCTCCT GGGGGTGGGCAGCCCTTGCTACAGCCTACCAAGGTAGATGCAGCTGAGGGCCGTCGGCCACAGGCCCTGC GGCTAATTGAGCAGGACCCCTATGAACATCCCGAGAGGCTGCAGCAGTTGCAGCAGGAGCTGGAGGCCTT TCGGGGCCAGCTGGGGGATGCAGGAGCACTGGATGCCATCTGGAGAGAGTTGCAAGAGGCACAGGAACAC GATGCCCGAGGTCGATCCATTGCCATTGCCCGCTGCTACTCCCTGAAGAACCGGCACCAGGATGTCATGC CGTATGACAGCAACCGTGTGGTGCTGCGCTCAGGCAAAGATGATTATATTAATGCTAGCTGTGTGGAGGG GCTCTCGCCATACTGTCCACCCTTAGTGGCCACCCAGGCTCCGCTGCCTGGCACAGCTGCAGATTTCTGG CTCATGGTGCATGAACAGAAAGTGTCAGTCATTGTCATGCTGGTGTCTGAGGCTGAGATGGAAAAGCAAA AGGTGGCGCGTTACTTCCCCACAGAGAGGGGCCAGCCCATGGTGCATGGAGCCCTGAGCGTGGCACTGAG CAGTATTCGTACTACTGAGACCCATGTGGAGCGAGTGCTGAGTCTGCAGTTCCGGGATCAGAGCCTCAAG CGCTCTCTTGTGCACCTCCATTTCCCTACTTGGCCTGAGTTAGGCCTGCCAGACAGCCCTGGCAATCTGC TGCGCTTCATCCAGGAGGTGCATGCACATTATTTGCACCAGCGACCCCTGCACACACCTATTGTCGTACA CTGCAGCTCTGGGGTGGGCCGCACAGGAGCCTTCGCGTTGCTCTATGCAGCCGTGCAGGAGGTGGAGGCT GGGAATGGGATTCCAGAGCTGCCCCAGCTGGTGAGGCGGATGCGGCAGCAGAGGAAACACATGCTGCAGG AAAAACTTCACCTCAAGTTCTGCCATGAGGCGTTGGTGAGACATGTGGAGCAGGTCCTACAGCGCCATGG TGTGCCTCCTCCTGGCAAGCCTGTGGCCAGTGTGAACATCAGCCAGAAGAACCACCTTCCTCAGGACTCC CAGGATCTGGTCCTTGGTGGAGACGTGCCCATCAGCTCCATTCAGGCTACCATTGCCAAGCTCAGCATCC GGCCCCTAGGTGGCTTGGATTCCCCAGCTGCCAGCCTCCCAGGCCTTGTAGAACCCCCAGGTCTTCCACC AGCTAGCCTTCCAGAGTCTACCCCTGTCCCATCTTCCTCGCCTCCCCCACTCTCTTCACCTCTGCCTGAA GCCCCCCAGCCAGAGGAGGAGCCGTCAGTGCCTGAAGCCCCCAGCTTAGGGCCCCCCTCGTCATCCCTGG AGCTGTTGGCCTCTCTGACTCCAGAAGCATTTTCACTGGACAGTTCTTTACGGGGAAAGCAGCGGATGAG CAAGCAGAACTTTCTGCAGGCCCACAACGGGCAGGGTCTAAGGGCTGCCCAGCCTACGGATGACCCCCTC AGCCTCCTGGATCCACTCTGGACACTCAACAAGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081043
Insert Size	5079 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081043.1, NP_001074512.1
RefSeq Size	5384 bp
RefSeq ORF	5079 bp
Locus ID	104831
UniProt ID	Q6PB44
Gene Summary	Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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