Col5a3 (NM_016919) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224890 representing NM_016919 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGCTGCCGGAGACTGGATCAGCTTCAGGCCGGCCTCTGCCTGCTCCTGGCCTCCCTGCAGCTCG TGTCCTGGACGCTGGCTGCAGAACCTGTGGACGTACTGGAAGCCTGGGGTGTGCATAGAGACCAGGCTGG GGTGGCTGAAGGGCCTGGCTTCTGCCCCCTGAGGATTCCACAGGGTGACCGAGCATTCAGGGTGGGCAAG TCCAGCCTTCTCAGTGTCCCCACGTGGCAGCTCTTCCCAGATGGGCATTTTCCTGAGAACTTTTCTGTGC TGCTCACACTGAGGGCCCAGCCAGCCAATCAGTCTGTCCTTCTGTCTATTTATGATGAGAAGGGTGTCCG GCAGCTGGGGCTGGCACTGGGGCCAGCTCTGGGCCTCCTTGGTGACTCCTTCAGGCCCCTCCCCAAGCAA GTCAACATTATGGATGGCAGGTGGCACCGTGTGGCAGTCAGCATCAGTGGTAACAAGGTGACCCTGGTGG TTGACTGTGAACCGCAGCCCCCAACATTTGGTCAGGGGCCTCGGTTTATAAGTACAGCTGGACTCACTGT GATGGGAACCCAGGACACCAGGGAAGAGTCTTTTGAGGGAGACATCCAGGAGCTGCTGTTAATTCCAGAC CCTCAGGCTGCCTTCCAGGCCTGTGAGAGCTACCTCCCTGGTTGTGAAACCCTCGATTCCACAACCACAG GGGCCCCCAAAGACGATGAACCAGAAACCCCTGCCCCTCGTCGTCGAAAGGGCAAAGGGAAGAAAAAAGG GCGGGGTCGAAAGGGCAAGGGAAGAAAGAAAAACAAGGAGACCTCAGAGCTGAGTCCGACCCCTGGTGCC CCTGAGAACCAGACCTCCCTCCACATCCCTGAGACAGAGAAGACAGTTCCCCACCTGCCTCTGACTCCCA CACCTCTGGCCATCACCACCACTGTCACGATTGGACAAAATGCCACAGTCTCGCAGGGGTTGGACTCCGG TACTGAAACCGAGCAGACGACTCCAGAGGTGGACTCTACTGAGGAGGGTGAAGGAGGTGGCCCCACCATG GGCCCCAAGTTCCGGGCAGCAGAGCAGTCCTTACAGACTGAGTTCCAGATCTTTCCTGGTGCTGGAGAAA AGGGAGCGAAAGGAGAGCCTGCGACAGTAGAGCAGGGACAGCAGTTTGAGGGGCCTGCAGGAGCTCCAGG ACCCCGGGGAATATCTGGTCCTTCAGGCCCTCCTGGGCCTCCGGGCTTCCCTGGGGACCGTGGTCTACCG GGTCCTGCCGGCCTCCCAGGAATCCCAGGCATCGATGGAGCCCGGGGCCTGCCGGGCACAGTGATTATGA TGCCGTTCCATTTTGCAAGCAGCTCGATGAAGGGACCCCCAGTGTCCTTCCAGCAGGCCCAGGCCCAGGC AGTATTGCAACAGGCTCAGCTGTCCATGAAAGGGCCCCCTGGTCCAGTAGGGCTCACTGGGCGCCCAGGC CCTGTGGGCCTCCCTGGATATCCAGGTCTGAAAGGTGAACTGGGAGAAGTGGGGCCACAGGGCCCCCGAG GATTACAGGGCCCTCCTGGGCCTCCTGGACGGGAAGGCAAGACAGGCCGAGCTGGAGCAGATGGGGCTCG GGGGCTCCCGGGAGACACAGGACCTAAGGGTGACAGGGGCTTTGATGGCCTGCCCGGGCTGCCTGGTGAG AAGGGCCAAAGGGGTGACTTTGGACGAGTAGGGCAACCTGGTCCCCCAGGAGAGGATGGTGTAAAGGGCC TGCAGGGACCTCCAGGGCCCACTGGCCAGGCTGGAGAGCCGGGTCCCCGAGGTCTGATTGGCCCCAGAGG TCTCCCAGGTCCCCTAGGACGCCCGGGTGTGACAGGGAGTGATGGCGCACCAGGGGCCAAAGGCAACGTG GGTCCTCCTGGAGAACCAGGACCCCCAGGACAGCAAGGAAACCACGGCTCCCAGGGAATTCCAGGCCCCC AGGGGCCCATTGGCACTCCCGGGGAAAAGGGTCCCCCTGGAAACCCCGGAATTCCAGGTGTCCCAGGATC TGAGGGCCCCCCGGGCCACCCAGGCCACGAGGGTCCCACAGGAGAAAAAGGGGCTCAGGGCCCACCAGGA TCAGCAGGCCCTCGGGGCTATCCTGGACTTCGTGGTGTGAAGGGTACCTCTGGTAACCGGGGTCTCCAAG GCGAGAAAGGAGAAAGGGGAGAGGATGGCTTTCCTGGCTTCAAGGGTGATGAGGGACCAAAAGGCGACCG GGGAAACCCCGGACCCCCAGGTCCCAGAGGAGAGGATGGTCCAGAAGGACAAAAGGGGCCTGGGGGACTG CCTGGTGATGAGGGTCCTCCAGGAGCAGCAGGGGAGAAGGGCAAGCTTGGGGTGCCAGGTCTCCCAGGTT ATCCAGGACGCCCAGGACCTAAGGGATCTATTGGATTTCCTGGACCCTTGGGACCACTGGGGGAGAAAGG CAAGCGGGGCAAAGCAGGACAGCCAGGAGAGGAAGGAGAACGCGGCACACCGGGCACCCGAGGAGACAGG GGACAGCCGGGGGCCACAGGCCAGCCTGGCCCCAAGGGTGACGTGGGCCAGAATGGGTCTCCTGGGCCCC CTGGAGAAAAGGGTCTACCCGGTCTTCAAGGCCCACCAGGATTCCCCGGACCAAAAGGCCCCCCGGGTCC TCAGGGGAAAGATGGGATATCTGGGCACCCTGGACAAAGAGGAGAATTGGGCTTCCAAGGTCTGACAGGC CCCCCTGGACCAGCTGGCGTCCTTGGTCCTCAGGGAAAGGTAGGGGACGTGGGGCCTCTAGGCGAGAGAG GCCCCCCAGGGCCTCCTGGACCTCCTGGTGAACAAGGTCTGCCAGGCATAGAAGGCAGAGAAGGGGCCAA GGGTGAGCTAGGACCCCTGGGGTCCGTCGGGAAGGAGGGGCCACCTGGGCCCAGGGGCTTCCCTGGCCCC CAAGGAGCCCCCGGAGACCCAGGACCCATTGGTTTGAAGGGTGACAAAGGTCCCCCAGGCCCTGTTGGGG CAAATGGCTCCCCGGGAGAGCGTGGTCCTGTAGGCCCCTCTGGTGGCATTGGGCTTCCTGGCCAGAGTGG AGGGCAAGGCCCTATTGGTCCTGCTGGCGAGAAGGGGTCCCCGGGAGAACGGGGTACTCCTGGTCCTACT GGCAAAGATGGTATTCCAGGACCCCCGGGGCTTCAGGGCCCCTCTGGAGCTGCGGGGCCTTCTGGGGAAG AAGGAGACAAGGGGGAAGTAGGGATGCCTGGTCACAAAGGAAGCAAAGGGGATAAAGGAGATGCAGGCCC ACCTGGACCAACAGGAATAAGAGGTCCAGCAGGCCATTCAGGCCTCCCGGGTGCTGATGGCGCTCAGGGT CGCCGGGGACCCCCTGGCCTCTTCGGGCAGAAGGGGGATGACGGAGTTCGAGGCTTTGTAGGTGTAATTG GTCCTCCAGGTCTGCAGGGGCTGCCGGGTCCTCCGGGGGAGAAGGGCGAGGTTGGAGACGTAGGATCCAT GGGTCCACATGGAGCTCCAGGCCCTCGGGGTCCCCCTGGGCCCAGTGGATCAGAGGGCCCCCCAGGTCTG CCTGGAGGAGTAGGACAGCCTGGTGCTGTGGGCGAGAAGGGTGAGCCAGGGGATGCTGGAGACGCCGGAC CCCCAGGAATTCCCGGCATCCCTGGGCCCAAAGGTGAAATTGGTGAAAAGGGGGATTCGGGTCCATCAGG GGCTGCTGGTCCCCCAGGCAAGAAAGGACCCCCAGGAGAGGACGGCTCTAAGGGGAACATGGGTCCCACA GGACTCCCTGGAGATCTAGGGCCCCCAGGAGACCCTGGAGTTCCGGGTATTGATGGCATCCCAGGGGAGA AGGGAAATGCTGGTGATATTGGGGGACCGGGGCCACCTGGAGCTTCCGGGGAACCTGGTGCCCGTGGCCT CCCTGGCAAGAGGGGTTCCCCTGGCCGCATGGGTCCAGAAGGAAGAGAGGGCGAGAAAGGCGCCAAGGGA GATGCTGGTCCTGATGGACCCCCAGGCAGGACAGGCCCCATTGGGGCTCGAGGGCCCCCTGGACGAATTG GGCCTGATGGTCTTCCAGGGATCCCTGGTCCTGTGGGTGAACCAGGTCTCCTGGGACCTCCTGGGCTAAT CGGCCCTCCAGGGCCCCTGGGCCCACCTGGCCTCCCTGGCCTGAAGGGAGATGCTGGCCCCAAGGGGGAG AAGGGCCACATTGGGCTAATAGGCCTCATTGGTCCCCCAGGGGAGGCCGGTGAGAAAGGCGATCAGGGGT TGCCAGGTGTGCAGGGCCCCCCAGGCCTTCAGGGAGACCCTGGTCTCCCTGGTCCTGTTGGCTCGTTAGG TCACCCTGGGCCCCCAGGTGTGGTGGGCCCTCTGGGACAGAAGGGCTCCAAAGGGTCCCCGGGATCTCTT GGTCCTCGTGGAGACCCTGGACCAGCGGGTCCTCCTGGTCCCCCGGGTTCTCCGGCTGAGGTGCATGGCC TGCGCAGGCGCCGATCTGTGACGGACACCCTGGAAGGTGGCCTGGAGGAGGTGATGGCCTCACTGAATTC ACTGAGCTTGGAGCTGCAGCAGTTGCAGAGACCTCTGGGCACAGCCGAGAGCCCAGGCCTCATGTGCCGA GAGCTTCACCGCGACCACCCACACCTGCCCGATGGAGAGTACTGGATTGACCCCAATCAGGGCTGTGCAC GTGACGCCTTCAAGGTTTTCTGCAACTTCACGGCAGGAGGTGAGACCTGTCTCTATCCAGACAAGAAGTT TGAGACGGTGAAACTGGCCTCGTGGTCCCGAGAGAAGCCTGGAGGCTGGTACAGCACCTTCCGCCGAGGG AAGAAGTTCTCCTATGTGGATGCTGATGGCTCCCCGGTGAATGTGGTCCAGTTGACCTTCCTGAAGTTGT TGAGTGCTGCAGCCCATCAGAGGTTCACTTACATCTGCCAGAACTCGGTGGCATGGCTGGATGAAGCTGC GGGTGACCACAGGCACTCCATCCGCTTCCAAGGGACCAACTGGGAAGAGTTGTCCTTCAACCAGACAACA GCAGCTACCATCAAGGTCTCCCATGATGGCTGTCGGGTCCGGAAGGGACAGGCGAAGACCCTCTTTGAAT TCAGCTCTTCTGTGGGTTTCCTGCCTCTGTGGGATGTGGCTGCCTCTGACTTTGGTCAGACGAACCAAAA GTTTGGGTTTGAACTCGGCTCCATCTGCTTTAGCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_016919
Insert Size	5220 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016919.3, NP_058615.1
RefSeq Size	6135 bp
RefSeq ORF	5220 bp
Locus ID	53867

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