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Col5a3 (NM_016919) Mouse Tagged ORF Clone
CAT＃: MG221630
- TrueORF®
Col5a3 (tGFP-tagged) - Mouse collagen type V alpha 3 (Col5a3), (10ug)
ORF Plasmid: DDK
"NM_016919" in other vectors (2)
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CNY 21,000.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221630 representing NM_016919
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGCTGCCGGAGACTGGATCAGCTTCAGGCCGGCCTCTGCCTGCTCCTGGCCTCCCTGCAGCTCG TGTCCTGGACGCTGGCTGCAGAACCTGTGGACGTACTGGAAGCCTGGGGTGTGCATAGAGACCAGGCTGG GGTGGCTGAAGGGCCTGGCTTCTGCCCCCTGAGGATTCCACAGGGTGACCGAGCATTCAGGGTGGGCAAG TCCAGCCTTCTCAGTGTCCCCACGTGGCAGCTCTTCCCAGATGGGCATTTTCCTGAGAACTTTTCTGTGC TGCTCACACTGAGGGCCCAGCCAGCCAATCAGTCTGTCCTTCTGTCTATTTATGATGAGAAGGGTGTCCG GCAGCTGGGGCTGGCACTGGGGCCAGCTCTGGGCCTCCTTGGTGACTCCTTCAGGCCCCTCCCCAAGCAA GTCAACATTATGGATGGCAGGTGGCACCGTGTGGCAGTCAGCATCAGTGGTAACAAGGTGACCCTGGTGG TTGACTGTGAACCGCAGCCCCCAACATTTGGTCAGGGGCCTCGGTTTATAAGTACAGCTGGACTCACTGT GATGGGAACCCAGGACACCAGGGAAGAGTCTTTTGAGGGAGACATCCAGGAGCTGCTGTTAATTCCAGAC CCTCAGGCTGCCTTCCAGGCCTGTGAGAGCTACCTCCCTGGTTGTGAAACCCTCGATTCCACAACCACAG GGGCCCCCAAAGACGATGAACCAGAAACCCCTGCCCCTCGTCGTCGAAAGGGCAAAGGGAAGAAAAAAGG GCGGGGTCGAAAGGGCAAGGGAAGAAAGAAAAACAAGGAGACCTCAGAGCTGAGTCCGACCCCTGGTGCC CCTGAGAACCAGACCTCCCTCCACATCCCTGAGACAGAGAAGACAGTTCCCCACCTGCCTCTGACTCCCA CACCTCTGGCCATCACCACCACTGTCACGATTGGACAAAATGCCACAGTCTCGCAGGGGTTGGACTCCGG TACTGAAACCGAGCAGACGACTCCAGAGGTGGACTCTACTGAGGAGGGTGAAGGAGGTGGCCCCACCATG GGCCCCAAGTTCCGGGCAGCAGAGCAGTCCTTACAGACTGAGTTCCAGATCTTTCCTGGTGCTGGAGAAA AGGGAGCGAAAGGAGAGCCTGCGACAGTAGAGCAGGGACAGCAGTTTGAGGGGCCTGCAGGAGCTCCAGG ACCCCGGGGAATATCTGGTCCTTCAGGCCCTCCTGGGCCTCCGGGCTTCCCTGGGGACCGTGGTCTACCG GGTCCTGCCGGCCTCCCAGGAATCCCAGGCATCGATGGAGCCCGGGGCCTGCCGGGCACAGTGATTATGA TGCCGTTCCATTTTGCAAGCAGCTCGATGAAGGGACCCCCAGTGTCCTTCCAGCAGGCCCAGGCCCAGGC AGTATTGCAACAGGCTCAGCTGTCCATGAAAGGGCCCCCTGGTCCAGTAGGGCTCACTGGGCGCCCAGGC CCTGTGGGCCTCCCTGGATATCCAGGTCTGAAAGGTGAACTGGGAGAAGTGGGGCCACAGGGCCCCCGAG GATTACAGGGCCCTCCTGGGCCTCCTGGACGGGAAGGCAAGACAGGCCGAGCTGGAGCAGATGGGGCTCG GGGGCTCCCGGGAGACACAGGACCTAAGGGTGACAGGGGCTTTGATGGCCTGCCCGGGCTGCCTGGTGAG AAGGGCCAAAGGGGTGACTTTGGACGAGTAGGGCAACCTGGTCCCCCAGGAGAGGATGGTGTAAAGGGCC TGCAGGGACCTCCAGGGCCCACTGGCCAGGCTGGAGAGCCGGGTCCCCGAGGTCTGATTGGCCCCAGAGG TCTCCCAGGTCCCCTAGGACGCCCGGGTGTGACAGGGAGTGATGGCGCACCAGGGGCCAAAGGCAACGTG GGTCCTCCTGGAGAACCAGGACCCCCAGGACAGCAAGGAAACCACGGCTCCCAGGGAATTCCAGGCCCCC AGGGGCCCATTGGCACTCCCGGGGAAAAGGGTCCCCCTGGAAACCCCGGAATTCCAGGTGTCCCAGGATC TGAGGGCCCCCCGGGCCACCCAGGCCACGAGGGTCCCACAGGAGAAAAAGGGGCTCAGGGCCCACCAGGA TCAGCAGGCCCTCGGGGCTATCCTGGACTTCGTGGTGTGAAGGGTACCTCTGGTAACCGGGGTCTCCAAG GCGAGAAAGGAGAAAGGGGAGAGGATGGCTTTCCTGGCTTCAAGGGTGATGAGGGACCAAAAGGCGACCG GGGAAACCCCGGACCCCCAGGTCCCAGAGGAGAGGATGGTCCAGAAGGACAAAAGGGGCCTGGGGGACTG CCTGGTGATGAGGGTCCTCCAGGAGCAGCAGGGGAGAAGGGCAAGCTTGGGGTGCCAGGTCTCCCAGGTT ATCCAGGACGCCCAGGACCTAAGGGATCTATTGGATTTCCTGGACCCTTGGGACCACTGGGGGAGAAAGG CAAGCGGGGCAAAGCAGGACAGCCAGGAGAGGAAGGAGAACGCGGCACACCGGGCACCCGAGGAGACAGG GGACAGCCGGGGGCCACAGGCCAGCCTGGCCCCAAGGGTGACGTGGGCCAGAATGGGTCTCCTGGGCCCC CTGGAGAAAAGGGTCTACCCGGTCTTCAAGGCCCACCAGGATTCCCCGGACCAAAAGGCCCCCCGGGTCC TCAGGGGAAAGATGGGATATCTGGGCACCCTGGACAAAGAGGAGAATTGGGCTTCCAAGGTCTGACAGGC CCCCCTGGACCAGCTGGCGTCCTTGGTCCTCAGGGAAAGGTAGGGGACGTGGGGCCTCTAGGCGAGAGAG GCCCCCCAGGGCCTCCTGGACCTCCTGGTGAACAAGGTCTGCCAGGCATAGAAGGCAGAGAAGGGGCCAA GGGTGAGCTAGGACCCCTGGGGTCCGTCGGGAAGGAGGGGCCACCTGGGCCCAGGGGCTTCCCTGGCCCC CAAGGAGCCCCCGGAGACCCAGGACCCATTGGTTTGAAGGGTGACAAAGGTCCCCCAGGCCCTGTTGGGG CAAATGGCTCCCCGGGAGAGCGTGGTCCTGTAGGCCCCTCTGGTGGCATTGGGCTTCCTGGCCAGAGTGG AGGGCAAGGCCCTATTGGTCCTGCTGGCGAGAAGGGGTCCCCGGGAGAACGGGGTACTCCTGGTCCTACT GGCAAAGATGGTATTCCAGGACCCCCGGGGCTTCAGGGCCCCTCTGGAGCTGCGGGGCCTTCTGGGGAAG AAGGAGACAAGGGGGAAGTAGGGATGCCTGGTCACAAAGGAAGCAAAGGGGATAAAGGAGATGCAGGCCC ACCTGGACCAACAGGAATAAGAGGTCCAGCAGGCCATTCAGGCCTCCCGGGTGCTGATGGCGCTCAGGGT CGCCGGGGACCCCCTGGCCTCTTCGGGCAGAAGGGGGATGACGGAGTTCGAGGCTTTGTAGGTGTAATTG GTCCTCCAGGTCTGCAGGGGCTGCCGGGTCCTCCGGGGGAGAAGGGCGAGGTTGGAGACGTAGGATCCAT GGGTCCACATGGAGCTCCAGGCCCTCGGGGTCCCCCTGGGCCCAGTGGATCAGAGGGCCCCCCAGGTCTG CCTGGAGGAGTAGGACAGCCTGGTGCTGTGGGCGAGAAGGGTGAGCCAGGGGATGCTGGAGACGCCGGAC CCCCAGGAATTCCCGGCATCCCTGGGCCCAAAGGTGAAATTGGTGAAAAGGGGGATTCGGGTCCATCAGG GGCTGCTGGTCCCCCAGGCAAGAAAGGACCCCCAGGAGAGGACGGCTCTAAGGGGAACATGGGTCCCACA GGACTCCCTGGAGATCTAGGGCCCCCAGGAGACCCTGGAGTTCCGGGTATTGATGGCATCCCAGGGGAGA AGGGAAATGCTGGTGATATTGGGGGACCGGGGCCACCTGGAGCTTCCGGGGAACCTGGTGCCCGTGGCCT CCCTGGCAAGAGGGGTTCCCCTGGCCGCATGGGTCCAGAAGGAAGAGAGGGCGAGAAAGGCGCCAAGGGA GATGCTGGTCCTGATGGACCCCCAGGCAGGACAGGCCCCATTGGGGCTCGAGGGCCCCCTGGACGAATTG GGCCTGATGGTCTTCCAGGGATCCCTGGTCCTGTGGGTGAACCAGGTCTCCTGGGACCTCCTGGGCTAAT CGGCCCTCCAGGGCCCCTGGGCCCACCTGGCCTCCCTGGCCTGAAGGGAGATGCTGGCCCCAAGGGGGAG AAGGGCCACATTGGGCTAATAGGCCTCATTGGTCCCCCAGGGGAGGCCGGTGAGAAAGGCGATCAGGGGT TGCCAGGTGTGCAGGGCCCCCCAGGCCTTCAGGGAGACCCTGGTCTCCCTGGTCCTGTTGGCTCGTTAGG TCACCCTGGGCCCCCAGGTGTGGTGGGCCCTCTGGGACAGAAGGGCTCCAAAGGGTCCCCGGGATCTCTT GGTCCTCGTGGAGACCCTGGACCAGCGGGTCCTCCTGGTCCCCCGGGTTCTCCGGCTGAGGTGCATGGCC TGCGCAGGCGCCGATCTGTGACGGACACCCTGGAAGGTGGCCTGGAGGAGGTGATGGCCTCACTGAATTC ACTGAGCTTGGAGCTGCAGCAGTTGCAGAGACCTCTGGGCACAGCCGAGAGCCCAGGCCTCATGTGCCGA GAGCTTCACCGCGACCACCCACACCTGCCCGATGGAGAGTACTGGATTGACCCCAATCAGGGCTGTGCAC GTGACGCCTTCAAGGTTTTCTGCAACTTCACGGCAGGAGGTGAGACCTGTCTCTATCCAGACAAGAAGTT TGAGACGGTGAAACTGGCCTCGTGGTCCCGAGAGAAGCCTGGAGGCTGGTACAGCACCTTCCGCCGAGGG AAGAAGTTCTCCTATGTGGATGCTGATGGCTCCCCGGTGAATGTGGTCCAGTTGACCTTCCTGAAGTTGT TGAGTGCTGCAGCCCATCAGAGGTTCACTTACATCTGCCAGAACTCGGTGGCATGGCTGGATGAAGCTGC GGGTGACCACAGGCACTCCATCCGCTTCCAAGGGACCAACTGGGAAGAGTTGTCCTTCAACCAGACAACA GCAGCTACCATCAAGGTCTCCCATGATGGCTGTCGGGTCCGGAAGGGACAGGCGAAGACCCTCTTTGAAT TCAGCTCTTCTGTGGGTTTCCTGCCTCTGTGGGATGTGGCTGCCTCTGACTTTGGTCAGACGAACCAAAA GTTTGGGTTTGAACTCGGCTCCATCTGCTTTAGCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221630 representing NM_016919
Red=Cloning site Green=Tags(s) MRSCRRLDQLQAGLCLLLASLQLVSWTLAAEPVDVLEAWGVHRDQAGVAEGPGFCPLRIPQGDRAFRVGK SSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDEKGVRQLGLALGPALGLLGDSFRPLPKQ VNIMDGRWHRVAVSISGNKVTLVVDCEPQPPTFGQGPRFISTAGLTVMGTQDTREESFEGDIQELLLIPD PQAAFQACESYLPGCETLDSTTTGAPKDDEPETPAPRRRKGKGKKKGRGRKGKGRKKNKETSELSPTPGA PENQTSLHIPETEKTVPHLPLTPTPLAITTTVTIGQNATVSQGLDSGTETEQTTPEVDSTEEGEGGGPTM GPKFRAAEQSLQTEFQIFPGAGEKGAKGEPATVEQGQQFEGPAGAPGPRGISGPSGPPGPPGFPGDRGLP GPAGLPGIPGIDGARGLPGTVIMMPFHFASSSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPG PVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFDGLPGLPGE KGQRGDFGRVGQPGPPGEDGVKGLQGPPGPTGQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNV GPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPG SAGPRGYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGL PGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDR GQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTG PPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGP QGAPGDPGPIGLKGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPT GKDGIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHSGLPGADGAQG RRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPPGPSGSEGPPGL PGGVGQPGAVGEKGEPGDAGDAGPPGIPGIPGPKGEIGEKGDSGPSGAAGPPGKKGPPGEDGSKGNMGPT GLPGDLGPPGDPGVPGIDGIPGEKGNAGDIGGPGPPGASGEPGARGLPGKRGSPGRMGPEGREGEKGAKG DAGPDGPPGRTGPIGARGPPGRIGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGPKGE KGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGSKGSPGSL GPRGDPGPAGPPGPPGSPAEVHGLRRRRSVTDTLEGGLEEVMASLNSLSLELQQLQRPLGTAESPGLMCR ELHRDHPHLPDGEYWIDPNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSREKPGGWYSTFRRG KKFSYVDADGSPVNVVQLTFLKLLSAAAHQRFTYICQNSVAWLDEAAGDHRHSIRFQGTNWEELSFNQTT AATIKVSHDGCRVRKGQAKTLFEFSSSVGFLPLWDVAASDFGQTNQKFGFELGSICFSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016919 |
| ORF Size | 5217 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016919.3, NP_058615.1 |
| RefSeq Size | 6086 bp |
| RefSeq ORF | 5220 bp |
| Locus ID | 53867 |
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