Dock2 (NM_033374) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI662014; AW122239; CED-5; Hch; MBC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224955 representing NM_033374 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCTGGCGCAAAACTGACAAGGAGCGTCACGGAGTGGCTATCTACAACTTCCAAGGCAGTGAAG CCCAGCATCTCACGCTACAGATTGGCGATGTGGTACGAATACAGGAGACCTGTGGAGACTGGTACAGAGG GTACCTCATAAAGCATAAACTGTCACAGGGCATTTTCCCTACATCCTTTATCCATCTCAAGGAAGTGACA GTGGAGAAGAGAAGGAACATAGAGAACATCATTCCTGCAGAAATCCCTCTGGCACAAGAAGTGACAACCA CGCTCTGGGAGTGGGGAAGCATCTGGAAGCAGCTCTATGTGGCCAGCAAAAAGGAACGCTTCCTCCAAGT GCAGTCCATGATGTACGACCTGATGGAATGGCGCTCTCAGCTCCTCTCAGGAACGCTACCCAAGGATGAG CTGAAGGAACTGAAGCAGAAAGTCACATCGAAGATCGACTATGGCAACAAAATCCTTGAGCTCGATCTCA TTGTCAGAGATGAAGATGGAAACATACTGGACCCTGATAAGACCAGCGTCATCAGCTTGTTCCATGCTCA CGAGGAGGCAACTTACAAAATCACAGAGCGCATCAAAGAAGAAATGTCAAAAGACCAGCCAGATTATGGG GTTTATTCTCGGATCTCCTCATCCCCCACCCACAGCCTCTACGTCTTTGTGAGAAACTTTGTGTGCCGAA TTGGGGAGGATGCTGAGCTCTTCATGTCTCTCTATGACCCTCACAAGCAAACGGTCATCAGTGAGAACTA TCTGGTGCGATGGGGCAGCAAAGGATTCCCAAAGGAAATTGAGATGCTCAATAACCTGAAGGTAGTCTTC ACGGATCTTGGAAATAAAGACCTCAACAGGGATAAGATTTTCTTGATCTGTCAAATAGTCCGAATTGGGA AGATGGATCTTAAGGACATTAATGCCAAGAAGTGCACCCAGGGGCTGAGGAGACCTTTTGGGGTGGCAGT TATGGACATCACCGACATCATCAAGGGCAAGGCAGAGAGCGACGAAGAGAAGCAACATTTTATTCCATTT CACCCGGTCTCAGCTGAGAACGATTTCCTTCACAGCCTTCTGGGCAAAGTCATAGCTTCCAAGGGAGACA GTGGAGGGCAAGGCCTTTGGGTGACCATGAAGATGCTGGTGGGGGACATCATTCAGATCCGTAAAGACTA CCCACACTTGGTGGACAGGACCACTGTGGTAGCCCGAAAGCTGGGCTTCCCAGAGATCATCATGCCAGGG GATGTCAGGAACGACATCTACATCACTCTCTTACAAGGTGACTTTGACAAGTACACCAAGACCACACAGC GGAACGTGGAAGTGATCATGTGTGTGTGCACAGAGGATGGCAAAGTGCTACCTAATGCAATTTGTGTGGG AGCGGGGGATAAGGCCATGAACGAGTACCACTCAGTCGTCTACTACCAAGTCAAACAGCCCCGATGGATG GAAACAGTCAAGGTGGCTGTCCCTATTGAAGATATGCAGAGGATCCATTTGAGGTTCATGTTTCGACACC GCTCATCACTAGAATCTAAAGATAAAGGAGAAAAGAACTTTGCCATGTCCTACGTGAAACTCATGAAAGA AGATGGAACCACTCTGCATGATGGATACCACGAGTTGGTGGTTCTCAAGGGAGACAGCAAGAAAATGGAA GATGCCAGCGCTTACCTGACACTTCCTTCTTATCGACACCCCGTGGAAAACAAGGGAGCTACGCTGAGCC GGAGCTCCAGCAGTGTTGGGGGCCTTTCTGTCAGCTCCCGGGATGTGTTCTCCATTTCCACGCTAGTGTG CTCCACAAAGCTGACCCAGAATGTGGGTTTGCTTGGCTTGCTGAAGTGGCGTATGAAGCCACAACTGCTC CAGGAGAATCTAGAGAAGTTGAAGATTGTGGATGGCGAGGAAGTAGTGAAGTTCCTCCAGGACACTCTGG ATGCCCTCTTCAACATCATGATGGAACACTCTCAGAGTAACGAGTATGACATCCTCGTCTTTGATGCTTT GATCTATATAATAGGACTCATTGCAGACCGGAAATTCCAGCATTTTAACACCGTATTGGAGGCTTACATC CAACAGCATTTCAGTGCTACCTTGGCCTACAAGAAACTGATGACGGTGCTGAAGACTTACTTGGATACCT CCAGCAGGGGGGAGCAGTGCGAGCCCATCCTCAGAACGCTCAAAGCCTTAGAATACGTGTTCAAGTTCAT TGTTCGGTCGAGGACATTATTCTCACAGCTTTACGAAGGCAAAGAGCAGATGGAGTTTGAAGAATCCATG AGACGGCTCTTCGAATCCATCAACAACCTGATGAAAAGTCAGTACAAGACCACCATCCTATTGCAGGTGG CGGCTTTGAAATACATCCCTTCAGTCCTTCACGATGTGGAAACAGTCTTTGATGCCAAGTTGCTCAGCCA GCTACTGTATGAGTTCTACACCTGCATCCCTCCCGTGAAGCTGCAGAAGCAGAAAGTTCAGTCCATGAAT GAGATCGTGCAGAGCAACCTCTTCAAAAAGCAAGAATGCCGGGACATTCTGCTTCCTGTCATCACCAAAG AGCTGAAGGAGCTGCTGGAGCAGAGGGACGATGGGCAGCACCAGGCTGAGAAGAAGCACTGTGTGGAACT TCTCAACAGCATCTTGGAGGTCCTCAGCTGTCAGGATGCGGCCTTCACTTATGACCACATCCAAGAGATC ATGGTCCAGCTGCTTCGCACAGTGAACCGGACAGTCATCACGATGGGCCGAGATCACGCTTTGATTAGTC ACTTTGTGGCGTGTATGACAGCTATCTTAGACCAGATGGGCGACCAACACTATTCATTTTACATTGAGAC CTTCCAGACCAGCTCAGACCTTGTGGACTTCTTGATGGAGACATTCATCATGTTCAAGGACCTCATTGGG AAGAATGTGTATCCTGGGGACTGGATGGCCATGAGCATGGTCCAGAACCGGGTCTTCCTGAGAGCCATCA ACAAGTTTGCAGAAACCATGAACCAGAAGTTTCTGGAACACACAAGCTTTGAGTTCCAGCTGTGGAACAA CTATTTTCATCTGGCAGTTGCCTTTATCACTCAGGACTCTCTGCAGCTGGAGCAGTTCACACACGCTAAG TACAACAAAATCCTGAATAAGTACGGGGACATGAGACGGCTCATAGGATTCTCCATCCGTGATATGTGGT ACAAGCTTGGCCAGAACAAAATCTGCTTCATCCCGGGCATGGTGGGACCTATATTAGAGATGACACTTAT CCCTGAGGCTGAGCTCAGGAAAGCCACCATCCCAATCTTCTTCGACATGATGCTGTGTGAATATCAAAGG ACTGGGGCTTTCAAAAAGTTTGAAAATGAAATCATCCTGAAGCTGGACCATGAGGTGGAAGGAGGCCGGG GGGATGAGCAGTACATGCAACTGCTGGAGTCCATCCTGATGGAGTGCACAGCGGAACACCCAACCATTGC CAAGTCGGTGGAGAACTTTGTGAGCTTGGTCAAAGGGCTGCTGGAGAAGTTGCTAGATTACCGGGGCGTG ATGACAGATGAGAGCAAAGACAACCGTATGAGCTGCACAGTGAACTTGCTGAATTTCTACAAAGATAACA ACCGGGAAGAGATGTACATAAGATACCTGTATAAACTGCGTGACCTTCACTTGGACTGTGAAAACTACAC AGAGGCTGCCTACACACTCCTCCTCCACACCTGGCTTCTCAAGTGGTCAGATGAGCAGTGTGCATCCCAG GTCATGCAGACAGGCCAGCAGCACCCTCAGACCCACCGGCAGCTGAAGGAGACACTCTATGAGACCATTA TAGGCTACTTTGACAAAGGAAAGATGTGGGAAGAGGCCATCAGCCTGTGCAAGGAACTGGCGGAACAATA TGAGATGGAGATCTTTGACTACGAGCTACTCAGCCAGAACCTGACCCAGCAGGCCAAATTCTATGAAAAC ATCATGAAAATCCTCAGAACCAAACCAGACTACTTTGCTGTTGGATACTATGGCCAGGGATTCCCCTCCT TTCTGCGGAACAAAGTGTTCATCTACCGAGGCAAGGAATATGAGCGAAGAGAAGACTTCCAGATGCAGCT TTTGAGCCAGTTCCCCAACGCAGAAAAGATGAACACAACTTCTGCCCCAGGAGACGATGTGAGGAATGCC CCAGGCCAGTACATCCAGTGTTTCACTGTGCAGCCTGTTCTGGATGAACACCCCAGGTTCAAGAACAAAC CGGTGCCTGACCAGATCATAAACTTTTACAAGTCTAATTATGTGCAAAAGTTCCACTACTCCAGGCCTGT GCGCAGGGGCAAGGTAGACCCAGAGAACGAGTTTGCTTCCATGTGGATCGAGAGGACTTCCTTCCTGACG GCCTACAAGCTGCCTGGCATCCTGCGCTGGTTTGAGGTAGTTCACATGTCTCAGACCACAATTAGTCCTC TGGAGAATGCCATCGAGACTATGTCCACAGTCAACGAGAAGATCCTGATGATGATAAACCAGTACCAGAG TGATGAAAGCCTCCCCATCAACCCTCTCTCCATGCTCCTCAATGGGATCGTGGACCCTGCTGTCATGGGA GGCTTCGCCAAGTATGAGAAGGCCTTCTTCACTGAAGAGTATAGCAGGGAGCACCCGGAGGACCAGGACA AGCTGAGCCATCTCAAGGACCTGATTGCATGGCAGATCCCTTTCCTGGGAGCTGGGATTAAGATCCACGA GAAAAGGGTGTCAGACAACCTGCGCCCCTTCCATGACCGGATGGAGGAGTGCTTCAAGAACCTGAAAATG AAGGTGGAAAAGGAGTATGGTGTCCGAGAGATGCCTGACTTCGAAGACAGGAGAGTGGGCCGCCCCAGGT CCATGCTGCGTTCCTACAGGCAGATGTCCGTCATCTCTCTGGCCTCCATGCATTCTGACTGCAGCACTCC CAGCAAAGTCCCCGCAGAAAGTTTTGACCTAGAGTCAGCCCCACCCAAGACTCCCAAAGTGGAGGAGGAG CCCATCTCCCCAGGGAGCACTCTGCCAGAGGTCAAGCTGCGGCGGTCCAAGAAAAGGACCAAGAGGAGCA GCGTGGTGTTTGCAGACGAGAAGGCAGCAACGGAGTCAGACCTGAAGCGGCTTTCTAGAAAGCAAGAGTT CATGAGTGACACCAACCTCTCAGAGCATGCAGCCATCCCTGCCAGGGTGTCTATCCTCTCTCAGATGAGT TTTGCCAGCCAGTCCATGCCCACCATCCCAGCCCTCACACTCTCCGTGGCAGGCGTGCCTGGGTTGGATG AGGCCAACACATCTCCCCGCCTGAGTCAGACCTTCTTCCAAGTCTCAGATGGTGACAAGAAGACCCTGAA AAAGAAGAAAGTCAATCAATTCTTCAAGACCATGCTGGCTAGCAAGTCGAGTGAAGAAAGCAAGCAGATC CCAGACTTCCTGTCCACCAACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_033374
Insert Size	5487 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_033374.3, NP_203538.2
RefSeq Size	6431 bp
RefSeq ORF	5487 bp
Locus ID	94176
UniProt ID	Q8C3J5
Gene Summary	Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.[UniProtKB/Swiss-Prot Function]

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