Cacna1s (NM_014193) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW493108; Cav1.1; Cchl1a3; fmd; mdg; sj
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224959 representing NM_014193 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCCCTCACCCCAGGACGAGGGCCTGAGGAAGAAGCAGCCGAAGAAACCTGTTCCTGAGATTC TGCCGAGGCCCCCGCGGGCCTTGTTCTGCCTGACTCTACAGAACCCCCTGAGGAAGGCCTGCATCAGCAT CGTGGAATGGAAACCCTTCGAGACCATCATCTTGCTCACCATCTTTGCCAACTGTGTGGCCCTGGCAGTG TACCTGCCCATGCCAGAGGATGACAACAACACTCTGAACCTCGGCCTGGAGAAACTGGAGTACTTCTTCC TCATTGTCTTCTCCATTGAAGCCGCCATGAAGATCATAGCCTACGGCTTCCTATTCCATCAGGATGCATA CCTGCGCAGTGGCTGGAACGTTCTGGACTTCATCATTGTCTTTCTGGGGGTCTTCACTGTGATTCTGGAA CAAGTTAACATCATTCAAACCAACACGGCCCCCATGAGCAGCAAAGGAGCCGGCTTGGATGTGAAGGCCC TTCGAGCATTCCGCGTGCTCAGACCCCTGCGGCTGGTGTCGGGGGTGCCCAGTCTTCAGGTGGTCCTCAA CTCCATCTTCAAGGCCATGCTCCCCCTGTTCCACATTGCCCTGCTCGTCCTCTTCATGGTCATCATCTAC GCCATCATTGGGCTGGAGCTCTTCAAAGGCAAGATGCACAAGACTTGCTACTTCATTGGGACAGACATTG TAGCCACAGTTGAGAATGAGAAACCATCACCCTGTGCTAGAACTGGCTCTGGGCGTCCCTGCACCATCAA TGGCAGCGAGTGCCGAGGAGGTTGGCCAGGACCCAACCACGGCATCACCCACTTCGACAACTTTGGCTTC TCTATGCTCACTGTGTATCAATGCATCTCCATGGAGGGGTGGACTGATGTCCTGTACTGGGTCAACGATG CCATCGGGAATGAGTGGCCCTGGATCTACTTTGTCACCCTCATTCTGCTGGGCTCCTTCTTCATCCTCAA CCTGGTGCTGGGTGTCCTGAGTGGAGAATTCACCAAAGAGCGAGAGAAGGCCAAGTCCAGGGGAACCTTC CAAAAGCTCCGAGAGAAACAGCAGCTGGAGGAGGACCTCCGTGGTTACATGAGCTGGATCACACAGGGCG AGGTCATGGACGTGGACGACTTGAGAGAAGGCAAGCTGTCTTTGGATGAAGGGGGATCAGACACAGAGAG CTTGTATGAAATCGAAGGCTTGAACAAAATCATCCAGTTCATTCGGCACTGGCGACAGTGGAATCGCGTC TTCCGCTGGAAGTGCCATGACCTAGTGAAATCCAAGGTCTTCTATTGGCTGGTGATCCTGATCGTTGCCC TAAACACCCTGTCCATTGCCTCGGAGCACCACAACCAGCCACTCTGGTTGACCCATTTGCAAGATGTTGC CAACCGCGTGTTGCTGACCCTCTTCACCATCGAAATGCTCATGAAGATGTACGGGCTGGGCCTGCGCCAG TACTTCATGTCCATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGCATCCTGGAGATCCTGCTGG TGGAGTCGGGAGCCATGTCGCCCCTGGGCATCTCTGTGCTCCGCTGTATCCGCCTCCTGAGGCTCTTCAA GATCACCAAGTACTGGACATCGCTGAGCAACCTGGTGGCCTCACTGCTCAACTCCATCCGCTCCATTGCT TCCCTCTTGCTGCTGCTCTTTCTCTTCATCATCATCTTTGCCCTGCTGGGCATGCAGCTCTTTGGAGGCA GATATGACTTTGAAGACACAGAAGTACGACGAAGCAACTTTGACAACTTCCCCCAGGCCCTCATCAGTGT CTTCCAGGTCCTGACGGGTGAAGACTGGAACTCTGTGATGTACAATGGGATCATGGCTTACGGAGGTCCG ACCTACCCTGGTGTGCTGGTGTGCATTTATTTCATCATCCTTTTCGTCTGTGGCAACTACATCCTTCTCA ACGTCTTCCTGGCTATTGCTGTGGACAACCTGGCAGAGGCAGAGAGTCTGACATCTGCCCAAAAGGCCAA GGCTGAGGAGAGAAAGCGCAGGAAGATGTCGAAAGGGCTCCCAGACAAGTCAGAAGAGGAGAGGGCCACG GTGACCAAGAAACTGGAGCAGAAGTCCAAGGGTGAAGGCATCCCCACCACCGCCAAGCTGAAAATCGATG AGTTTGAATCAAACGTTAATGAGGTGAAAGACCCCTACCCTTCGGCTGACTTCCCAGGGGACGATGAGGA AGATGAGCCCGAGATCCCAGTGAGCCCCCGACCACGCCCTCTGGCCGAGCTGCAGCTGAAAGAGAAAGCA GTGCCCATCCCGGAGGCTAGCTCCTTCTTCATCTTCAGTCCCACCAACAAGATCCGTGTCCTGTGTCACC GCATTGTCAATGCCACCTGGTTCACCAACTTCATCCTGCTCTTCATCCTGCTCAGCAGTGCCGCCCTGGC CGCTGAGGATCCCATCCGGGCCGACTCCATGAGGAATCAGATCCTCGAGTATTTTGATTATGTGTTCACT GCTGTTTTCACGGTGGAGATTGTCCTTAAGATGACCACCTATGGAGCCTTCCTGCACAAAGGCTCCTTCT GCCGCAACTACTTCAACATCCTGGACCTGCTGGTGGTGGCTGTGTCCCTCATCTCCATGGGCCTCGAGTC CAGCGCCATCTCCGTGGTGAAGATCTTAAGGGTTCTGAGAGTGCTGAGACCACTCCGGGCCATAAACAGA GCCAAAGGGTTGAAGCACGTGGTGCAGTGCGTGTTTGTTGCCATTCGCACCATCGGGAACATCGTGCTGG TCACCACGCTCCTGCAGTTCATGTTTGCGTGCATTGGAGTGCAGCTTTTCAAGGGAAAGTTCTACAGCTG CAATGACTTATCCAAGATGACTGAAGAGGAATGCAGGGGCTACTACTACATATACAAGGATGGAGACCCC ACTCAGATAGAGCTGCGTCCCCGCCAGTGGATACACAATGACTTCCACTTTGACAATGTACTCTCCGCTA TGATGTCACTCTTCACGGTCTCCACCTTCGAGGGTTGGCCTCAGCTACTGTACAAGGCCATAGACTCAAA TGAGGAGGACACCGGCCCTGTCTACAACAACCGAGTGGAGATGGCCATCTTCTTCATTATCTACATCATC CTCATTGCCTTCTTCATGATGAACATCTTTGTGGGCTTTGTCATCGTTACCTTCCAGGAACAGGGAGAGA CTGAGTATAAGAACTGTGAGCTCGACAAGAACCAGCGCCAATGCGTACAGTATGCCCTGAAGGCCCGCCC ACTGAGGTGTTACATCCCCAAAAACCCATACCAGTATCAGGTGTGGTATGTCGTCACTTCCTCCTACTTT GAATACCTGATGTTTGCTCTCATCATGCTCAATACTATCTGCCTGGGCATGCAGCATTACAACCAGTCGG AACAGATGAACCACATCTCAGACATTCTCAACGTGGCTTTCACCATCATCTTCACCCTGGAGATGGTCCT CAAGCTCATAGCTTTCAAGCCCAGGGGCTATTTTGGAGATCCTTGGAATGTGTTTGACTTCCTAATCGTC ATCGGCAGCATCATTGACGTCATCCTGAGCGAGATCGATGACCCAGATGAGAGCGCCCGTATCTCCAGTG CCTTCTTCCGCCTGTTCCGGGTCATGAGACTGGTCAAGCTGCTGAACCGGGCGGAGGGTGTGCGCACCCT GCTGTGGACATTCATCAAGTCCTTCCAGGCCCTGCCGTACGTGGCTTTGCTGATCGTGATGCTCTTCTTC ATCTACGCGGTCATCGGCATGCAGATGTTCGGGAAGATCGCCATGGTGGACGGGACGCAGATAAACCGGA ACAACAACTTCCAGACTTTCCCACAGGCCGTGCTGCTGCTCTTCAGGTGTGCCACAGGGGAAGCCTGGCA AGAGATCCTGCTGGCCTGCAGCTATGGGAAGCTGTGTGACCCGGAGTCTGACTACGCACCCGGGGAGGAG CATACATGCGGCACCAACTTCGCCTACTACTACTTCATCAGTTTCTACATGCTCTGCGCCTTCCTGATCA TCAACCTCTTTGTGGCTGTCATCATGGACAACTTTGATTACCTCACAAGGGACTGGTCCATTCTGGGCCC TCACCACCTGGACGAGTTCAAGGCCATTTGGGCTGAGTACGACCCGGAGGCTAAGGGCAGGATCAAGCAC CTGGATGTGGTGACCCTGCTGAGGAGGATTCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACACAGGG TAGCATGTAAGAGGCTGGTGGGCATGAACATGCCCCTGAACAGCGATGGCACAGTCACCTTTAATGCCAC ACTATTTGCCCTGGTGCGCACAGCCCTCAAGATCAAGACAGAAGGCAACTTTGAACAAGCTAACGAGGAG CTGAGAGCCATCATCAAGAAGATCTGGAAGAGAACCAGCATGAAGCTCTTGGACCAAGTCATCCCTCCAA TAGGAGATGATGAGGTGACAGTAGGGAAATTCTATGCCACATTCCTCATCCAGGAGCACTTCCGGAAGTT CATGAAGCGCCAGGAGGAATATTATGGGTATCGACCCAAAAAGGACACAGTTCAGATACAGGCTGGATTA CGGACCATAGAGGAAGAGGCAGCTCCTGAGATCCACCGTGCCATCTCTGGAGACCTGACAGCAGAGGAGG AACTGGAGAGGGCCATGGTAGAGGCTGCCATGGAGGAGGGGATATTCCGGAGGACGGGAGGCCTGTTTGG TCAAGTGGACAACTTCCTGGAAAGGACCAACTCTCTGCCCCCTGTCATGGCCAACCAAAGACCCCTCCAA TTTGCTGAAATAGAGATGGAAGAGCTGGAGTCACCAGTCTTCTTGGAGGACTTCCCTCAGAACCCAGGCA CCCACCCTCTTGCTCGTGCCAATACCAACAACGCCAATGCCAATGTTGCCTATGGCAACAGCAGCCATAG AAACAACCCAGTGTTTTCCAGCATCTGCTATGAACGGGAGTTCTTGGGAGAGGCGGACATGCCTGTTACC AGAGAAGGACCCCTCAGTCAGCCCTGCAGGGCCTCAGGACCTCACAGCAGATCTCATGTGGACAAACTGA AAAGACCGATGACCCAGAGAGGAATGCCTGAAGGCCAGGTGCCTCCTTCCCCGTGCCAGCTCTCCCAGGC AGAGCATCCTGTGCAGAAAGAGGGAAAGGGCCCCACGTCACGGTTTCTGGAAACACCCAACAGCAGGAAC TTTGAGGAGCACGTTCCCAGGAATTCGGCTCACAGGTGCACGGCTCCAGCTACTGCCATGCTGATCCAGG AGGCTCTGGTTCGAGGAGGACTAGACTCCTTAGCAGCAGATGCTAACTTCGTCATGGCAACAGGCCAGGC CCTGGCAGATGCCTGCCAGATGGAACCAGAGGAAGTCGAGGTGGCAGCAACAGAGCTACTGAAGCAAGAA TCCCCAGAGGGCGGGGCCGTGCCCTGGGAGCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_014193
Insert Size	5496 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014193.2, NP_055008.2
RefSeq Size	6188 bp
RefSeq ORF	5496 bp
Locus ID	12292

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