Zgrf1 (NM_197997) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930422G04Rik; AI448607
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224991 representing NM_197997 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATGTCAAGAGTTTATTGTCCTATATACTCATCAAAAGATGAAGAAGTCCAAAGTGTGGCAAGATG GAGTTCTGAAGATCACTCACTTAGGCAATAAGGCGATTTTATATGATGACAAAGGAGCATGTTTGGAAAG CCTGTTTCTTAAGTGCCTTGAGGTGAAACCTGGAGATGACTTGGAAAGTGAGCGGTATTTAATCACAGTT GAGGAGGCTAAGGCTGTTGGAAGCAGAGCTGTTGAACCGGATGGCTCTAGAGAAGCACTGGAGTCAGGCT CAAGGACGCTTGTATCCTCCAGCAGGTCTCTTGGGTGTCAGCCCTCTGGCTTAAAAAGGAAGGCTACTGG TTTTCAAAGACCATATAAGATGCCAAAGAAAGTGACTATTACTGAGAACAGTGAACCTGCTGCCTCACTT GGTGATGAGAACCCTGGCCCGCCTGGCCCGAGACTTCTTCCCACATTCTCCAGCACACTTCCTTTGTTTC CCACTGTTGGTCAGAAAGATTTAACTCCTGTGTCAACGGATAATCAGAGTCCCATCACATTCAGCAACAG AGAGAGAAGCGACACACCTTTATCACTTCCTTCTTCATACTTCAAGATTAACACAAACACACTAGGTAAA GAGGACAAGCTTTGCTTTCCTGTCAGTTCTGAAACCAAACATTCAGACTCTTTACTGGCCAGTGAACCCA TGAGAAGAAATGGTTTAGACTCTCACTGTCCTGGAGTTTCACAAAACGTCAGAAGCAAAGCCCAGATATT AGCTCTTCTGAAGTCCAGCTCAACTAACAGGAAGGATCTGCATGGTGAGATTCCAGGACACTTTCCTAAG ATAGAACCACAGGGGTGTTTGAATATTATTTCTAAGCCAGAGGAAGACTATGCAGAAACACAGAGCATAG GGAATTTACGCTGTGAGCAGCAGTCAGAAAATCCTACAAGAACCACAAGCCGGTGGGCCAGGTATTTACC TTCCCAGAGATCACCTCCCTGTTCTGCTGCAGATGAGAATGATACAGAGGACAAACCAGAAGCCCAAGAA GATGTGAACATTTTTAACTTGTCTGAACTTTTACAAAAAAAGTCAGAGCTCTTTGAAACATGTTCCAAAA AGGGAGAATTACACAGTGAGGACAAGCCAGTAGATAATACTTGCCAATACTGGAATCAGGAGGATAACTT AGCACCTTCATTCTGTAAGAACAGCAGTGTCCTGGTTAGCTGCAGCAGTAAAGAGAATGCCAGTTTATTA TCAGAATCTGACATTCAGTACAGTAGCAAAGTACCAGTTAATCAACATGAGAAGGTGTGGACTCGAGAAG GTTCTCAGGGGGCAGATGCGGATGCAGCATTGGAACCGGAGTATAGACCAGTGTCACCTTTGCCAGAAAT AGGACATAAACAAACTGAAGTTGAAGCTTCTCTAAGTACCAGCTCTAGGATCTCTGACGACATTGCTGAC ATGGGTTCTAAAAGCAATGCTGATAGAGAAGATCTGAAAACTGTTCATAAAGCTGTGCAACCATTTTTGG AGGTTTCTTTTAATCTGAGCAACTTTGAGACCAGTGACACTGATGAGGCGTCACAAGAAGACAGCAGACT TTCCCAGGATTCAGAGCGGTGGGAGAAGGAAGCTGTGCTTACAGATGATTCTTGTGTTCAGAAGAGCTGT GAGGATATAGGCTGCCGAGAAATTGTAGGAAAATTGCCACTCCTGTCATCTACCGATGACGAGCCTAAAG AGGCCCTCCCTGCTGATGGGACTCTGTTGTCAGAGTTCTGTGATAGAACCTGTGTGGGTTTCAATTCAGG ACCTCATGGAGATGTAAAAACTGGCAAAGCACTAGAGGGACAGTGTCATAGTGACACAGGGAGCAGTTTG GACTCGTCGCTGGAGTGGAGTGAGGATGTAGCAGGGGACAGCAGAGAAGATGCTAGTCAATCTATCCAAG GCAATGCAATTAATTGTGGTGGTGTTTCACCCTCAAAGAAACCCAGAGGTGTAAACAGAAGCTTATACAG TCCTTATCTTCTAACCGCAGTCACTGACCCAGCGCCTGAGAACAGTGACCTGCTCTCAGAAGCCAGGAAA TCACCTGCCATAGAAGTTTCGCGTGCTCTGCTAGAGTGTCCTTGTGAGCATCGGGCTTCTCAGCAGCCTG TTGAGTTTCAAGGACATCAGGTGAAAGGATCCGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTTACA GCGAGGATGCACTCAGTTTCCAGACAGCATTGACTATGAAAACTTCCTGACTGATACTTGTGTTTGGACA CCAGGATTGCCTAGCACTTATGGGCAGACGGACTTCTTGCAGGTGATTTCTCCAGAACAAAAGATCCCTG CATTGAGCCCTGCACCTACTTTTTCTTTCAACACAAGAAATGAAGACACTGTGCTTGAGCTCTCTGAGGA GTCTCTGAAAACAAGAACTCTCCCTGGATTGAAAACCATTGGTTTCCAGGACAGTAAGAACCTCCAACGG TTTCCGTTTCTAAGTGGAGCCTCAGCTCCCTTTGCCACTTTGCCAGCTGATGATGGCCCAGCTGTCTTAG ATCCCTGTTCATTCATGATTGATGATGACGCGCGAGAGCCTTCTGGTTCTTCCATGTTGAACCTGTGTGA AGAGTCAGGTCTTTCATTCGACCTTGGACTTGAGGGCCAGGGTGGGACTCCAGGAGGTGTTTCACTGCTT CCTAAGAGTTCTACTCAAAGCAAGTGGCTGAAATATCAAAACCCACCCCAGTGCAACTCAACTGCTCCGA ACAGACTTGCCAGCGAGGTGACCGAGGGTCTCTTTGCTGAAGCGGTGTCTGGCTTGCATTTTAGCCACAC AAGTGAGAGTGAAAGTTCTGTTGACCCTGTGCGCCTGCAGATGATAAAAGGCTTGCTCCATCAACAGCAG CAGGATTTGGTTTCCAGAAAGCAAGCTTTTTCTCTGACTTTAAATCAGACTTGCAAGACTCAGGAACATG AGACTGTGTTAGGAAGCTCTGCCAGCAAGAACTGCAGAGCCAAAGATCTACAGGAGATAAATAATTCTGA TCTGTGCTTCCCAAATGGGCAGAAAATAATATCAGCTTATCTCCCACAAAGGCAAGTTCACATACCTGCT GTTTTCCAGTCTCCTGCTCACTATAGGCAGGTCTTTACAGCTTCCATCATAGAGCATCTGAATATATTGC TGTTTGAATTGGCACAAAGGCTGTACAAAGCACTTTCCAAAGTTGACATATCCTTTTATACATCGTCAAA AGGAGAGACAATGAGAAGTGGAAAAAATAATTCACCATCCTGCCACCATAATCAACCTGCCAAGCTTGTC ATGGTTAAAAAAGAAGGTCCAAATAAGGGCCGTCTCTTTTATACATGTGATAAGTCAAAAGATAATCAAT GTAAATTTTTCAAATGGCTTGAGGAAGTGACTCCAGGACAGTTACCACAGAATACGTCTCAATCCACCAT GGTTTTTAATGATATTAAGAGTATTGGCTCATATTTAAGAAGTCAAAAGGTACCAGTCTATGAAGAATGC CAGCTTTTGTTGAGGAGAGGATTTGATTTTCAGAGAAAACAGTGTGGTAAACTAAAGAAGCTAACTACTG TAAATCCTGACTTTTACAGTGAAACAAAAAGTAAGATTTATCTTAAGCTGAGTCGGAGGGAGAGCTCTTC AGTTTATAGTAAAGGTGATCTTTGGGTTATTTCAAAAACCCTAGACTTTGAACTAGATACTTTCATTGCA TGCAGTGCCTTCTTTGGGCCATCTTCTGTCAATGAAGTGGAGCTGCTGCCTTTGAAAGGTTATTTCCCTT CTAATTGGCCCACCAACATCACAGTCCATGCATTGTTGGTTTGTAATGCCAGCACAGAGCTGACAACTCT ACAGAACATTCAGGACTACTTTAACCCAGCTGCTCTTCCACTAATGCCATACCTGCTAGCAATGTCTCAG TCAGCTACAGTTAGCAGTAAAAATATCAGTAAAAGAAAATTTATCCCACCAGCCATTACAAGCATTAAAA CAAAAACGGAACTCCACCTTGGAGCGACCCTGCAGTTAGCTAGGGAGTTGATTAACGTACACAGGTTGAA CAAGGACCAAGCTACAGCACTAATTCAGATAGCTCAAATGATGGCATCCCAAGGAAGTGATGAAGATGCC CTGGAGCCCTTCGGCCATTCCCTGCCTATCACAGTCATTCATGGTGTTTTTGGAGCAGGCAAAAGCTATT TGCTGGCTGTGGTGATTCTGTTCTTGGTAGAGCTGTTTGAAAAGTGTGATTCTGGAACAGTTGGCAATGC AAGGCCATGGAAAGTCCTCGTTTCTTCTTCCACTAACGTAGCTGTTGACAGAGTGCTTCTTGGGCTTCTC AGTCTTGGATTTGAAAAGTTCATCAGGGTTGGAAGTGTTAGGAAGATCGCCAAGCCAGTTTTACCCTACA GCTTGCATGCTGGCTCAGACAATGAAAGTGAACAATTGAAGGAACTCAATGCACTGTTGAAGGAAGAACT GACTCCTATTGAAAGAGTTTATGTCAGAAAAAGTATCGAGCAGCATAAGCTGGGAACCAACAGAGTCCTG CTAAAGCAGGTCCGAGTGGTTGGCGTTACCTGTGCAGCCTGCGCATTCCCGTGTCTGAATGACCTTAAGT TCCCCGTGGTTGTGCTGGATGAGTGCAGCCAGATGACAGAGCCAGCCTCCCTTCTTCCCATTGCAAGGTT TCAGTGTGAAAAGCTAATTCTTGTTGGAGACCCCAAACAGCTGCCTCCTACAATTCAGGGTTCTGATGCA GCTCATGAAAATGGACTGGAACAAACTCTCTTTGATCGGCTTTGCTTAATGGGCCACAAACCCGTTCTGT TAAGAACCCAGTACCGTTGTCATCCTGCGATCAGTGCCATAGCTAATGATCTGTTTTATGAAGGAAGCCT AGTGAATGGCATTTCCGAGAGAGAGAGAAGCCCTGTACTGGAGTGGCTGCCCACGCTGTGTTTCTATAAC GTCACGGGAGCAGAGCAGGTTGAGAGAGAAAACAGCTTTGTGAATGTGGCAGAAGCCACGTTTACCCTCA AGCTAATCCAATCACTGATGGCAAGTGGGGTAGAGAGCTGCATGATTGGGGTGATAACATTATACAAGTC GCAGATGTACAAGATTTGCAATTTACTCAGTGCTGTGGACGTCGGCCATCCTGATGTTAAAGCTGTGCAG GTGTCCACAGTGGATGCCTTTCAGGGTGCCGAGAAGGAAATCACTATTCTGTCCTGCGTGAGGACAAGAC AAGTAGGGTTCATTGACTCAGAGAAACGAATGAACGTTGCTTTGACCAGAGGAAGGAGACACTTATTGAT TGTAGGAAGTTTGTCCTGTTTGAGGAAAAATCGACTATGGGGGCGTGTGATCCAACACTGTGAAGGAAGG GAAGACGGATTGCAGCATGCAAGCCAGTGTGAACCACAGCTGGACCATCTTCTTAAAGACTATTTAGAAA AACAAGCAGAAGAAAAACAGAAAAAAAAGGAGAAAGAGAAATCAAAGGATAAATCTCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_197997
Insert Size	5592 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_197997.2, NP_932114.2
RefSeq Size	7064 bp
RefSeq ORF	5592 bp
Locus ID	71643
UniProt ID	Q0VGT4

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