Zgrf1 (NM_197997) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4930422G04Rik; AI448607
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215000 representing NM_197997 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATGTCAAGAGTTTATTGTCCTATATACTCATCAAAAGATGAAGAAGTCCAAAGTGTGGCAAGATG GAGTTCTGAAGATCACTCACTTAGGCAATAAGGCGATTTTATATGATGACAAAGGAGCATGTTTGGAAAG CCTGTTTCTTAAGTGCCTTGAGGTGAAACCTGGAGATGACTTGGAAAGTGAGCGGTATTTAATCACAGTT GAGGAGGCTAAGGCTGTTGGAAGCAGAGCTGTTGAACCGGATGGCTCTAGAGAAGCACTGGAGTCAGGCT CAAGGACGCTTGTATCCTCCAGCAGGTCTCTTGGGTGTCAGCCCTCTGGCTTAAAAAGGAAGGCTACTGG TTTTCAAAGACCATATAAGATGCCAAAGAAAGTGACTATTACTGAGAACAGTGAACCTGCTGCCTCACTT GGTGATGAGAACCCTGGCCCGCCTGGCCCGAGACTTCTTCCCACATTCTCCAGCACACTTCCTTTGTTTC CCACTGTTGGTCAGAAAGATTTAACTCCTGTGTCAACGGATAATCAGAGTCCCATCACATTCAGCAACAG AGAGAGAAGCGACACACCTTTATCACTTCCTTCTTCATACTTCAAGATTAACACAAACACACTAGGTAAA GAGGACAAGCTTTGCTTTCCTGTCAGTTCTGAAACCAAACATTCAGACTCTTTACTGGCCAGTGAACCCA TGAGAAGAAATGGTTTAGACTCTCACTGTCCTGGAGTTTCACAAAACGTCAGAAGCAAAGCCCAGATATT AGCTCTTCTGAAGTCCAGCTCAACTAACAGGAAGGATCTGCATGGTGAGATTCCAGGACACTTTCCTAAG ATAGAACCACAGGGGTGTTTGAATATTATTTCTAAGCCAGAGGAAGACTATGCAGAAACACAGAGCATAG GGAATTTACGCTGTGAGCAGCAGTCAGAAAATCCTACAAGAACCACAAGCCGGTGGGCCAGGTATTTACC TTCCCAGAGATCACCTCCCTGTTCTGCTGCAGATGAGAATGATACAGAGGACAAACCAGAAGCCCAAGAA GATGTGAACATTTTTAACTTGTCTGAACTTTTACAAAAAAAGTCAGAGCTCTTTGAAACATGTTCCAAAA AGGGAGAATTACACAGTGAGGACAAGCCAGTAGATAATACTTGCCAATACTGGAATCAGGAGGATAACTT AGCACCTTCATTCTGTAAGAACAGCAGTGTCCTGGTTAGCTGCAGCAGTAAAGAGAATGCCAGTTTATTA TCAGAATCTGACATTCAGTACAGTAGCAAAGTACCAGTTAATCAACATGAGAAGGTGTGGACTCGAGAAG GTTCTCAGGGGGCAGATGCGGATGCAGCATTGGAACCGGAGTATAGACCAGTGTCACCTTTGCCAGAAAT AGGACATAAACAAACTGAAGTTGAAGCTTCTCTAAGTACCAGCTCTAGGATCTCTGACGACATTGCTGAC ATGGGTTCTAAAAGCAATGCTGATAGAGAAGATCTGAAAACTGTTCATAAAGCTGTGCAACCATTTTTGG AGGTTTCTTTTAATCTGAGCAACTTTGAGACCAGTGACACTGATGAGGCGTCACAAGAAGACAGCAGACT TTCCCAGGATTCAGAGCGGTGGGAGAAGGAAGCTGTGCTTACAGATGATTCTTGTGTTCAGAAGAGCTGT GAGGATATAGGCTGCCGAGAAATTGTAGGAAAATTGCCACTCCTGTCATCTACCGATGACGAGCCTAAAG AGGCCCTCCCTGCTGATGGGACTCTGTTGTCAGAGTTCTGTGATAGAACCTGTGTGGGTTTCAATTCAGG ACCTCATGGAGATGTAAAAACTGGCAAAGCACTAGAGGGACAGTGTCATAGTGACACAGGGAGCAGTTTG GACTCGTCGCTGGAGTGGAGTGAGGATGTAGCAGGGGACAGCAGAGAAGATGCTAGTCAATCTATCCAAG GCAATGCAATTAATTGTGGTGGTGTTTCACCCTCAAAGAAACCCAGAGGTGTAAACAGAAGCTTATACAG TCCTTATCTTCTAACCGCAGTCACTGACCCAGCGCCTGAGAACAGTGACCTGCTCTCAGAAGCCAGGAAA TCACCTGCCATAGAAGTTTCGCGTGCTCTGCTAGAGTGTCCTTGTGAGCATCGGGCTTCTCAGCAGCCTG TTGAGTTTCAAGGACATCAGGTGAAAGGATCCGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTTACA GCGAGGATGCACTCAGTTTCCAGACAGCATTGACTATGAAAACTTCCTGACTGATACTTGTGTTTGGACA CCAGGATTGCCTAGCACTTATGGGCAGACGGACTTCTTGCAGGTGATTTCTCCAGAACAAAAGATCCCTG CATTGAGCCCTGCACCTACTTTTTCTTTCAACACAAGAAATGAAGACACTGTGCTTGAGCTCTCTGAGGA GTCTCTGAAAACAAGAACTCTCCCTGGATTGAAAACCATTGGTTTCCAGGACAGTAAGAACCTCCAACGG TTTCCGTTTCTAAGTGGAGCCTCAGCTCCCTTTGCCACTTTGCCAGCTGATGATGGCCCAGCTGTCTTAG ATCCCTGTTCATTCATGATTGATGATGACGCGCGAGAGCCTTCTGGTTCTTCCATGTTGAACCTGTGTGA AGAGTCAGGTCTTTCATTCGACCTTGGACTTGAGGGCCAGGGTGGGACTCCAGGAGGTGTTTCACTGCTT CCTAAGAGTTCTACTCAAAGCAAGTGGCTGAAATATCAAAACCCACCCCAGTGCAACTCAACTGCTCCGA ACAGACTTGCCAGCGAGGTGACCGAGGGTCTCTTTGCTGAAGCGGTGTCTGGCTTGCATTTTAGCCACAC AAGTGAGAGTGAAAGTTCTGTTGACCCTGTGCGCCTGCAGATGATAAAAGGCTTGCTCCATCAACAGCAG CAGGATTTGGTTTCCAGAAAGCAAGCTTTTTCTCTGACTTTAAATCAGACTTGCAAGACTCAGGAACATG AGACTGTGTTAGGAAGCTCTGCCAGCAAGAACTGCAGAGCCAAAGATCTACAGGAGATAAATAATTCTGA TCTGTGCTTCCCAAATGGGCAGAAAATAATATCAGCTTATCTCCCACAAAGGCAAGTTCACATACCTGCT GTTTTCCAGTCTCCTGCTCACTATAGGCAGGTCTTTACAGCTTCCATCATAGAGCATCTGAATATATTGC TGTTTGAATTGGCACAAAGGCTGTACAAAGCACTTTCCAAAGTTGACATATCCTTTTATACATCGTCAAA AGGAGAGACAATGAGAAGTGGAAAAAATAATTCACCATCCTGCCACCATAATCAACCTGCCAAGCTTGTC ATGGTTAAAAAAGAAGGTCCAAATAAGGGCCGTCTCTTTTATACATGTGATAAGTCAAAAGATAATCAAT GTAAATTTTTCAAATGGCTTGAGGAAGTGACTCCAGGACAGTTACCACAGAATACGTCTCAATCCACCAT GGTTTTTAATGATATTAAGAGTATTGGCTCATATTTAAGAAGTCAAAAGGTACCAGTCTATGAAGAATGC CAGCTTTTGTTGAGGAGAGGATTTGATTTTCAGAGAAAACAGTGTGGTAAACTAAAGAAGCTAACTACTG TAAATCCTGACTTTTACAGTGAAACAAAAAGTAAGATTTATCTTAAGCTGAGTCGGAGGGAGAGCTCTTC AGTTTATAGTAAAGGTGATCTTTGGGTTATTTCAAAAACCCTAGACTTTGAACTAGATACTTTCATTGCA TGCAGTGCCTTCTTTGGGCCATCTTCTGTCAATGAAGTGGAGCTGCTGCCTTTGAAAGGTTATTTCCCTT CTAATTGGCCCACCAACATCACAGTCCATGCATTGTTGGTTTGTAATGCCAGCACAGAGCTGACAACTCT ACAGAACATTCAGGACTACTTTAACCCAGCTGCTCTTCCACTAATGCCATACCTGCTAGCAATGTCTCAG TCAGCTACAGTTAGCAGTAAAAATATCAGTAAAAGAAAATTTATCCCACCAGCCATTACAAGCATTAAAA CAAAAACGGAACTCCACCTTGGAGCGACCCTGCAGTTAGCTAGGGAGTTGATTAACGTACACAGGTTGAA CAAGGACCAAGCTACAGCACTAATTCAGATAGCTCAAATGATGGCATCCCAAGGAAGTGATGAAGATGCC CTGGAGCCCTTCGGCCATTCCCTGCCTATCACAGTCATTCATGGTGTTTTTGGAGCAGGCAAAAGCTATT TGCTGGCTGTGGTGATTCTGTTCTTGGTAGAGCTGTTTGAAAAGTGTGATTCTGGAACAGTTGGCAATGC AAGGCCATGGAAAGTCCTCGTTTCTTCTTCCACTAACGTAGCTGTTGACAGAGTGCTTCTTGGGCTTCTC AGTCTTGGATTTGAAAAGTTCATCAGGGTTGGAAGTGTTAGGAAGATCGCCAAGCCAGTTTTACCCTACA GCTTGCATGCTGGCTCAGACAATGAAAGTGAACAATTGAAGGAACTCAATGCACTGTTGAAGGAAGAACT GACTCCTATTGAAAGAGTTTATGTCAGAAAAAGTATCGAGCAGCATAAGCTGGGAACCAACAGAGTCCTG CTAAAGCAGGTCCGAGTGGTTGGCGTTACCTGTGCAGCCTGCGCATTCCCGTGTCTGAATGACCTTAAGT TCCCCGTGGTTGTGCTGGATGAGTGCAGCCAGATGACAGAGCCAGCCTCCCTTCTTCCCATTGCAAGGTT TCAGTGTGAAAAGCTAATTCTTGTTGGAGACCCCAAACAGCTGCCTCCTACAATTCAGGGTTCTGATGCA GCTCATGAAAATGGACTGGAACAAACTCTCTTTGATCGGCTTTGCTTAATGGGCCACAAACCCGTTCTGT TAAGAACCCAGTACCGTTGTCATCCTGCGATCAGTGCCATAGCTAATGATCTGTTTTATGAAGGAAGCCT AGTGAATGGCATTTCCGAGAGAGAGAGAAGCCCTGTACTGGAGTGGCTGCCCACGCTGTGTTTCTATAAC GTCACGGGAGCAGAGCAGGTTGAGAGAGAAAACAGCTTTGTGAATGTGGCAGAAGCCACGTTTACCCTCA AGCTAATCCAATCACTGATGGCAAGTGGGGTAGAGAGCTGCATGATTGGGGTGATAACATTATACAAGTC GCAGATGTACAAGATTTGCAATTTACTCAGTGCTGTGGACGTCGGCCATCCTGATGTTAAAGCTGTGCAG GTGTCCACAGTGGATGCCTTTCAGGGTGCCGAGAAGGAAATCACTATTCTGTCCTGCGTGAGGACAAGAC AAGTAGGGTTCATTGACTCAGAGAAACGAATGAACGTTGCTTTGACCAGAGGAAGGAGACACTTATTGAT TGTAGGAAGTTTGTCCTGTTTGAGGAAAAATCGACTATGGGGGCGTGTGATCCAACACTGTGAAGGAAGG GAAGACGGATTGCAGCATGCAAGCCAGTGTGAACCACAGCTGGACCATCTTCTTAAAGACTATTTAGAAA AACAAGCAGAAGAAAAACAGAAAAAAAAGGAGAAAGAGAAATCAAAGGATAAATCTCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215000 representing NM_197997 Red=Cloning site Green=Tags(s) MECQEFIVLYTHQKMKKSKVWQDGVLKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESERYLITV EEAKAVGSRAVEPDGSREALESGSRTLVSSSRSLGCQPSGLKRKATGFQRPYKMPKKVTITENSEPAASL GDENPGPPGPRLLPTFSSTLPLFPTVGQKDLTPVSTDNQSPITFSNRERSDTPLSLPSSYFKINTNTLGK EDKLCFPVSSETKHSDSLLASEPMRRNGLDSHCPGVSQNVRSKAQILALLKSSSTNRKDLHGEIPGHFPK IEPQGCLNIISKPEEDYAETQSIGNLRCEQQSENPTRTTSRWARYLPSQRSPPCSAADENDTEDKPEAQE DVNIFNLSELLQKKSELFETCSKKGELHSEDKPVDNTCQYWNQEDNLAPSFCKNSSVLVSCSSKENASLL SESDIQYSSKVPVNQHEKVWTREGSQGADADAALEPEYRPVSPLPEIGHKQTEVEASLSTSSRISDDIAD MGSKSNADREDLKTVHKAVQPFLEVSFNLSNFETSDTDEASQEDSRLSQDSERWEKEAVLTDDSCVQKSC EDIGCREIVGKLPLLSSTDDEPKEALPADGTLLSEFCDRTCVGFNSGPHGDVKTGKALEGQCHSDTGSSL DSSLEWSEDVAGDSREDASQSIQGNAINCGGVSPSKKPRGVNRSLYSPYLLTAVTDPAPENSDLLSEARK SPAIEVSRALLECPCEHRASQQPVEFQGHQVKGSATSGVMVRGHSLQRGCTQFPDSIDYENFLTDTCVWT PGLPSTYGQTDFLQVISPEQKIPALSPAPTFSFNTRNEDTVLELSEESLKTRTLPGLKTIGFQDSKNLQR FPFLSGASAPFATLPADDGPAVLDPCSFMIDDDAREPSGSSMLNLCEESGLSFDLGLEGQGGTPGGVSLL PKSSTQSKWLKYQNPPQCNSTAPNRLASEVTEGLFAEAVSGLHFSHTSESESSVDPVRLQMIKGLLHQQQ QDLVSRKQAFSLTLNQTCKTQEHETVLGSSASKNCRAKDLQEINNSDLCFPNGQKIISAYLPQRQVHIPA VFQSPAHYRQVFTASIIEHLNILLFELAQRLYKALSKVDISFYTSSKGETMRSGKNNSPSCHHNQPAKLV MVKKEGPNKGRLFYTCDKSKDNQCKFFKWLEEVTPGQLPQNTSQSTMVFNDIKSIGSYLRSQKVPVYEEC QLLLRRGFDFQRKQCGKLKKLTTVNPDFYSETKSKIYLKLSRRESSSVYSKGDLWVISKTLDFELDTFIA CSAFFGPSSVNEVELLPLKGYFPSNWPTNITVHALLVCNASTELTTLQNIQDYFNPAALPLMPYLLAMSQ SATVSSKNISKRKFIPPAITSIKTKTELHLGATLQLARELINVHRLNKDQATALIQIAQMMASQGSDEDA LEPFGHSLPITVIHGVFGAGKSYLLAVVILFLVELFEKCDSGTVGNARPWKVLVSSSTNVAVDRVLLGLL SLGFEKFIRVGSVRKIAKPVLPYSLHAGSDNESEQLKELNALLKEELTPIERVYVRKSIEQHKLGTNRVL LKQVRVVGVTCAACAFPCLNDLKFPVVVLDECSQMTEPASLLPIARFQCEKLILVGDPKQLPPTIQGSDA AHENGLEQTLFDRLCLMGHKPVLLRTQYRCHPAISAIANDLFYEGSLVNGISERERSPVLEWLPTLCFYN VTGAEQVERENSFVNVAEATFTLKLIQSLMASGVESCMIGVITLYKSQMYKICNLLSAVDVGHPDVKAVQ VSTVDAFQGAEKEITILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGSLSCLRKNRLWGRVIQHCEGR EDGLQHASQCEPQLDHLLKDYLEKQAEEKQKKKEKEKSKDKSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_197997
ORF Size	5589 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_197997.2, NP_932114.2
RefSeq Size	7064 bp
RefSeq ORF	5592 bp
Locus ID	71643
UniProt ID	Q0VGT4
MW	206.2 kDa

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