Zgrf1 (NM_197997) Mouse Tagged ORF Clone
CAT#: MR215000
- TrueORF®
4930422G04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930422G04 gene (4930422G04Rik)
ORF Plasmid: tGFP
"NM_197997" in other vectors (2)
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CNY 12,520.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4930422G04Rik; AI448607 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215000 representing NM_197997
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATGTCAAGAGTTTATTGTCCTATATACTCATCAAAAGATGAAGAAGTCCAAAGTGTGGCAAGATG GAGTTCTGAAGATCACTCACTTAGGCAATAAGGCGATTTTATATGATGACAAAGGAGCATGTTTGGAAAG CCTGTTTCTTAAGTGCCTTGAGGTGAAACCTGGAGATGACTTGGAAAGTGAGCGGTATTTAATCACAGTT GAGGAGGCTAAGGCTGTTGGAAGCAGAGCTGTTGAACCGGATGGCTCTAGAGAAGCACTGGAGTCAGGCT CAAGGACGCTTGTATCCTCCAGCAGGTCTCTTGGGTGTCAGCCCTCTGGCTTAAAAAGGAAGGCTACTGG TTTTCAAAGACCATATAAGATGCCAAAGAAAGTGACTATTACTGAGAACAGTGAACCTGCTGCCTCACTT GGTGATGAGAACCCTGGCCCGCCTGGCCCGAGACTTCTTCCCACATTCTCCAGCACACTTCCTTTGTTTC CCACTGTTGGTCAGAAAGATTTAACTCCTGTGTCAACGGATAATCAGAGTCCCATCACATTCAGCAACAG AGAGAGAAGCGACACACCTTTATCACTTCCTTCTTCATACTTCAAGATTAACACAAACACACTAGGTAAA GAGGACAAGCTTTGCTTTCCTGTCAGTTCTGAAACCAAACATTCAGACTCTTTACTGGCCAGTGAACCCA TGAGAAGAAATGGTTTAGACTCTCACTGTCCTGGAGTTTCACAAAACGTCAGAAGCAAAGCCCAGATATT AGCTCTTCTGAAGTCCAGCTCAACTAACAGGAAGGATCTGCATGGTGAGATTCCAGGACACTTTCCTAAG ATAGAACCACAGGGGTGTTTGAATATTATTTCTAAGCCAGAGGAAGACTATGCAGAAACACAGAGCATAG GGAATTTACGCTGTGAGCAGCAGTCAGAAAATCCTACAAGAACCACAAGCCGGTGGGCCAGGTATTTACC TTCCCAGAGATCACCTCCCTGTTCTGCTGCAGATGAGAATGATACAGAGGACAAACCAGAAGCCCAAGAA GATGTGAACATTTTTAACTTGTCTGAACTTTTACAAAAAAAGTCAGAGCTCTTTGAAACATGTTCCAAAA AGGGAGAATTACACAGTGAGGACAAGCCAGTAGATAATACTTGCCAATACTGGAATCAGGAGGATAACTT AGCACCTTCATTCTGTAAGAACAGCAGTGTCCTGGTTAGCTGCAGCAGTAAAGAGAATGCCAGTTTATTA TCAGAATCTGACATTCAGTACAGTAGCAAAGTACCAGTTAATCAACATGAGAAGGTGTGGACTCGAGAAG GTTCTCAGGGGGCAGATGCGGATGCAGCATTGGAACCGGAGTATAGACCAGTGTCACCTTTGCCAGAAAT AGGACATAAACAAACTGAAGTTGAAGCTTCTCTAAGTACCAGCTCTAGGATCTCTGACGACATTGCTGAC ATGGGTTCTAAAAGCAATGCTGATAGAGAAGATCTGAAAACTGTTCATAAAGCTGTGCAACCATTTTTGG AGGTTTCTTTTAATCTGAGCAACTTTGAGACCAGTGACACTGATGAGGCGTCACAAGAAGACAGCAGACT TTCCCAGGATTCAGAGCGGTGGGAGAAGGAAGCTGTGCTTACAGATGATTCTTGTGTTCAGAAGAGCTGT GAGGATATAGGCTGCCGAGAAATTGTAGGAAAATTGCCACTCCTGTCATCTACCGATGACGAGCCTAAAG AGGCCCTCCCTGCTGATGGGACTCTGTTGTCAGAGTTCTGTGATAGAACCTGTGTGGGTTTCAATTCAGG ACCTCATGGAGATGTAAAAACTGGCAAAGCACTAGAGGGACAGTGTCATAGTGACACAGGGAGCAGTTTG GACTCGTCGCTGGAGTGGAGTGAGGATGTAGCAGGGGACAGCAGAGAAGATGCTAGTCAATCTATCCAAG GCAATGCAATTAATTGTGGTGGTGTTTCACCCTCAAAGAAACCCAGAGGTGTAAACAGAAGCTTATACAG TCCTTATCTTCTAACCGCAGTCACTGACCCAGCGCCTGAGAACAGTGACCTGCTCTCAGAAGCCAGGAAA TCACCTGCCATAGAAGTTTCGCGTGCTCTGCTAGAGTGTCCTTGTGAGCATCGGGCTTCTCAGCAGCCTG TTGAGTTTCAAGGACATCAGGTGAAAGGATCCGCTACTAGTGGTGTAATGGTCAGAGGACACAGCTTACA GCGAGGATGCACTCAGTTTCCAGACAGCATTGACTATGAAAACTTCCTGACTGATACTTGTGTTTGGACA CCAGGATTGCCTAGCACTTATGGGCAGACGGACTTCTTGCAGGTGATTTCTCCAGAACAAAAGATCCCTG CATTGAGCCCTGCACCTACTTTTTCTTTCAACACAAGAAATGAAGACACTGTGCTTGAGCTCTCTGAGGA GTCTCTGAAAACAAGAACTCTCCCTGGATTGAAAACCATTGGTTTCCAGGACAGTAAGAACCTCCAACGG TTTCCGTTTCTAAGTGGAGCCTCAGCTCCCTTTGCCACTTTGCCAGCTGATGATGGCCCAGCTGTCTTAG ATCCCTGTTCATTCATGATTGATGATGACGCGCGAGAGCCTTCTGGTTCTTCCATGTTGAACCTGTGTGA AGAGTCAGGTCTTTCATTCGACCTTGGACTTGAGGGCCAGGGTGGGACTCCAGGAGGTGTTTCACTGCTT CCTAAGAGTTCTACTCAAAGCAAGTGGCTGAAATATCAAAACCCACCCCAGTGCAACTCAACTGCTCCGA ACAGACTTGCCAGCGAGGTGACCGAGGGTCTCTTTGCTGAAGCGGTGTCTGGCTTGCATTTTAGCCACAC AAGTGAGAGTGAAAGTTCTGTTGACCCTGTGCGCCTGCAGATGATAAAAGGCTTGCTCCATCAACAGCAG CAGGATTTGGTTTCCAGAAAGCAAGCTTTTTCTCTGACTTTAAATCAGACTTGCAAGACTCAGGAACATG AGACTGTGTTAGGAAGCTCTGCCAGCAAGAACTGCAGAGCCAAAGATCTACAGGAGATAAATAATTCTGA TCTGTGCTTCCCAAATGGGCAGAAAATAATATCAGCTTATCTCCCACAAAGGCAAGTTCACATACCTGCT GTTTTCCAGTCTCCTGCTCACTATAGGCAGGTCTTTACAGCTTCCATCATAGAGCATCTGAATATATTGC TGTTTGAATTGGCACAAAGGCTGTACAAAGCACTTTCCAAAGTTGACATATCCTTTTATACATCGTCAAA AGGAGAGACAATGAGAAGTGGAAAAAATAATTCACCATCCTGCCACCATAATCAACCTGCCAAGCTTGTC ATGGTTAAAAAAGAAGGTCCAAATAAGGGCCGTCTCTTTTATACATGTGATAAGTCAAAAGATAATCAAT GTAAATTTTTCAAATGGCTTGAGGAAGTGACTCCAGGACAGTTACCACAGAATACGTCTCAATCCACCAT GGTTTTTAATGATATTAAGAGTATTGGCTCATATTTAAGAAGTCAAAAGGTACCAGTCTATGAAGAATGC CAGCTTTTGTTGAGGAGAGGATTTGATTTTCAGAGAAAACAGTGTGGTAAACTAAAGAAGCTAACTACTG TAAATCCTGACTTTTACAGTGAAACAAAAAGTAAGATTTATCTTAAGCTGAGTCGGAGGGAGAGCTCTTC AGTTTATAGTAAAGGTGATCTTTGGGTTATTTCAAAAACCCTAGACTTTGAACTAGATACTTTCATTGCA TGCAGTGCCTTCTTTGGGCCATCTTCTGTCAATGAAGTGGAGCTGCTGCCTTTGAAAGGTTATTTCCCTT CTAATTGGCCCACCAACATCACAGTCCATGCATTGTTGGTTTGTAATGCCAGCACAGAGCTGACAACTCT ACAGAACATTCAGGACTACTTTAACCCAGCTGCTCTTCCACTAATGCCATACCTGCTAGCAATGTCTCAG TCAGCTACAGTTAGCAGTAAAAATATCAGTAAAAGAAAATTTATCCCACCAGCCATTACAAGCATTAAAA CAAAAACGGAACTCCACCTTGGAGCGACCCTGCAGTTAGCTAGGGAGTTGATTAACGTACACAGGTTGAA CAAGGACCAAGCTACAGCACTAATTCAGATAGCTCAAATGATGGCATCCCAAGGAAGTGATGAAGATGCC CTGGAGCCCTTCGGCCATTCCCTGCCTATCACAGTCATTCATGGTGTTTTTGGAGCAGGCAAAAGCTATT TGCTGGCTGTGGTGATTCTGTTCTTGGTAGAGCTGTTTGAAAAGTGTGATTCTGGAACAGTTGGCAATGC AAGGCCATGGAAAGTCCTCGTTTCTTCTTCCACTAACGTAGCTGTTGACAGAGTGCTTCTTGGGCTTCTC AGTCTTGGATTTGAAAAGTTCATCAGGGTTGGAAGTGTTAGGAAGATCGCCAAGCCAGTTTTACCCTACA GCTTGCATGCTGGCTCAGACAATGAAAGTGAACAATTGAAGGAACTCAATGCACTGTTGAAGGAAGAACT GACTCCTATTGAAAGAGTTTATGTCAGAAAAAGTATCGAGCAGCATAAGCTGGGAACCAACAGAGTCCTG CTAAAGCAGGTCCGAGTGGTTGGCGTTACCTGTGCAGCCTGCGCATTCCCGTGTCTGAATGACCTTAAGT TCCCCGTGGTTGTGCTGGATGAGTGCAGCCAGATGACAGAGCCAGCCTCCCTTCTTCCCATTGCAAGGTT TCAGTGTGAAAAGCTAATTCTTGTTGGAGACCCCAAACAGCTGCCTCCTACAATTCAGGGTTCTGATGCA GCTCATGAAAATGGACTGGAACAAACTCTCTTTGATCGGCTTTGCTTAATGGGCCACAAACCCGTTCTGT TAAGAACCCAGTACCGTTGTCATCCTGCGATCAGTGCCATAGCTAATGATCTGTTTTATGAAGGAAGCCT AGTGAATGGCATTTCCGAGAGAGAGAGAAGCCCTGTACTGGAGTGGCTGCCCACGCTGTGTTTCTATAAC GTCACGGGAGCAGAGCAGGTTGAGAGAGAAAACAGCTTTGTGAATGTGGCAGAAGCCACGTTTACCCTCA AGCTAATCCAATCACTGATGGCAAGTGGGGTAGAGAGCTGCATGATTGGGGTGATAACATTATACAAGTC GCAGATGTACAAGATTTGCAATTTACTCAGTGCTGTGGACGTCGGCCATCCTGATGTTAAAGCTGTGCAG GTGTCCACAGTGGATGCCTTTCAGGGTGCCGAGAAGGAAATCACTATTCTGTCCTGCGTGAGGACAAGAC AAGTAGGGTTCATTGACTCAGAGAAACGAATGAACGTTGCTTTGACCAGAGGAAGGAGACACTTATTGAT TGTAGGAAGTTTGTCCTGTTTGAGGAAAAATCGACTATGGGGGCGTGTGATCCAACACTGTGAAGGAAGG GAAGACGGATTGCAGCATGCAAGCCAGTGTGAACCACAGCTGGACCATCTTCTTAAAGACTATTTAGAAA AACAAGCAGAAGAAAAACAGAAAAAAAAGGAGAAAGAGAAATCAAAGGATAAATCTCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215000 representing NM_197997
Red=Cloning site Green=Tags(s) MECQEFIVLYTHQKMKKSKVWQDGVLKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESERYLITV EEAKAVGSRAVEPDGSREALESGSRTLVSSSRSLGCQPSGLKRKATGFQRPYKMPKKVTITENSEPAASL GDENPGPPGPRLLPTFSSTLPLFPTVGQKDLTPVSTDNQSPITFSNRERSDTPLSLPSSYFKINTNTLGK EDKLCFPVSSETKHSDSLLASEPMRRNGLDSHCPGVSQNVRSKAQILALLKSSSTNRKDLHGEIPGHFPK IEPQGCLNIISKPEEDYAETQSIGNLRCEQQSENPTRTTSRWARYLPSQRSPPCSAADENDTEDKPEAQE DVNIFNLSELLQKKSELFETCSKKGELHSEDKPVDNTCQYWNQEDNLAPSFCKNSSVLVSCSSKENASLL SESDIQYSSKVPVNQHEKVWTREGSQGADADAALEPEYRPVSPLPEIGHKQTEVEASLSTSSRISDDIAD MGSKSNADREDLKTVHKAVQPFLEVSFNLSNFETSDTDEASQEDSRLSQDSERWEKEAVLTDDSCVQKSC EDIGCREIVGKLPLLSSTDDEPKEALPADGTLLSEFCDRTCVGFNSGPHGDVKTGKALEGQCHSDTGSSL DSSLEWSEDVAGDSREDASQSIQGNAINCGGVSPSKKPRGVNRSLYSPYLLTAVTDPAPENSDLLSEARK SPAIEVSRALLECPCEHRASQQPVEFQGHQVKGSATSGVMVRGHSLQRGCTQFPDSIDYENFLTDTCVWT PGLPSTYGQTDFLQVISPEQKIPALSPAPTFSFNTRNEDTVLELSEESLKTRTLPGLKTIGFQDSKNLQR FPFLSGASAPFATLPADDGPAVLDPCSFMIDDDAREPSGSSMLNLCEESGLSFDLGLEGQGGTPGGVSLL PKSSTQSKWLKYQNPPQCNSTAPNRLASEVTEGLFAEAVSGLHFSHTSESESSVDPVRLQMIKGLLHQQQ QDLVSRKQAFSLTLNQTCKTQEHETVLGSSASKNCRAKDLQEINNSDLCFPNGQKIISAYLPQRQVHIPA VFQSPAHYRQVFTASIIEHLNILLFELAQRLYKALSKVDISFYTSSKGETMRSGKNNSPSCHHNQPAKLV MVKKEGPNKGRLFYTCDKSKDNQCKFFKWLEEVTPGQLPQNTSQSTMVFNDIKSIGSYLRSQKVPVYEEC QLLLRRGFDFQRKQCGKLKKLTTVNPDFYSETKSKIYLKLSRRESSSVYSKGDLWVISKTLDFELDTFIA CSAFFGPSSVNEVELLPLKGYFPSNWPTNITVHALLVCNASTELTTLQNIQDYFNPAALPLMPYLLAMSQ SATVSSKNISKRKFIPPAITSIKTKTELHLGATLQLARELINVHRLNKDQATALIQIAQMMASQGSDEDA LEPFGHSLPITVIHGVFGAGKSYLLAVVILFLVELFEKCDSGTVGNARPWKVLVSSSTNVAVDRVLLGLL SLGFEKFIRVGSVRKIAKPVLPYSLHAGSDNESEQLKELNALLKEELTPIERVYVRKSIEQHKLGTNRVL LKQVRVVGVTCAACAFPCLNDLKFPVVVLDECSQMTEPASLLPIARFQCEKLILVGDPKQLPPTIQGSDA AHENGLEQTLFDRLCLMGHKPVLLRTQYRCHPAISAIANDLFYEGSLVNGISERERSPVLEWLPTLCFYN VTGAEQVERENSFVNVAEATFTLKLIQSLMASGVESCMIGVITLYKSQMYKICNLLSAVDVGHPDVKAVQ VSTVDAFQGAEKEITILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGSLSCLRKNRLWGRVIQHCEGR EDGLQHASQCEPQLDHLLKDYLEKQAEEKQKKKEKEKSKDKSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_197997 |
ORF Size | 5589 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_197997.2, NP_932114.2 |
RefSeq Size | 7064 bp |
RefSeq ORF | 5592 bp |
Locus ID | 71643 |
UniProt ID | Q0VGT4 |
MW | 206.2 kDa |
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Product Manuals |
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SDS |