Dock1 (NM_001033420) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130006G06Rik; AI854900; D630004B07Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224993 representing NM_001033420 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGCTGGGTCCCCACCAAGCGAGAGGAGAAGTACGGCGTGGCCTTTTACAACTACGATGCCAGGG GAGCAGATGAACTGTCCCTTCAAATTGGAGACACCGTGCACATCCTAGAAACTTATGAAGGGTGGTACAG AGGCTACACCTTAAGGAAAAAGTCTAAAAAGGGTATATTTCCTGCTTCATATATTCATCTTAAAGAAGCC ATTGTTGAAGGAAAAGGGCAACATGAAACCGTCATCCCTGGGGACCTCCCCCTTATACAGGAAGTCACCA CAACGCTTCGAGAGTGGTCTACCATCTGGAGGCAACTTTATGTGCAAGATAATAGAGAGATGTTTCGAAG TGTGCGACACATGATCTATGACCTTATTGAATGGCGGTCGCAGATCCTTTCTGGAACTCTGCCCCAGGAT GAGCTCAAAGAGCTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTCTTGATTTGGACC TGGTGGTGAGGGATGAAGATGGGAACATTTTGGACCCGGAACTAACTAGCACCATCAGTCTCTTCCGAGC ACACGAAGTTGCTTCTAAACAAGTGGAGGAGAGACTGCAAGAGGAGAAATCTCAAAAGCAGAACATGGAT ATCAACAGACAAGCCAAGTTTGCAGCCACCCCATCGCTGGCCTTGTTTGTTAACCTCAAAAATGTGGTTT GTAAAATAGGAGAAGATGCTGAAGTTCTCATGTCTCTTTATGACCCCATGGAGTCCAAATTCATCAGTGA GAACTATCTGGTCCGCTGGTCAAGTTCAGGATTACCAAAAGATATTGATCGGTTACATAACTTGCGAGCT GTGTTTACGGATCTGGGGAGCAAAGACTTGAAAAGGGAGAAAATTAGTTTCGTCTGCCAGATTGTCCGTG TCGGTCGCATGGAGCTGAGGGACAGCAACACGAGGAAGCTGACTTCGGGGCTGCGGCGACCTTTCGGCGT GGCTGTGATGGATGTGACAGACATAATAAACGGGAAAGTCGATGATGAAGATAAACAGCATTTCATCCCC TTCCAGGCGGTGGCAGGGGAGAATGACTTCCTTCAAACTGTCATAAACAAAGTCATTGCAGCCAAAGAAG TCAACCACAAGGGACAAGGTTTGTGGGTAACCTTGAAATTACTTCCTGGAGACATCCATCAGATTCGGAA GGAGTTTCCTCACTTGGTGGACAGGACCACAGCTGTGGCCCGCAAGACAGGGTTTCCTGAGATTATTATG CCAGGTGATGTTCGAAATGATATCTATGTGACATTAGTTCAAGGGGATTTTGATAAAGGCAGTAAAACAA CTGCGAAGAACGTGGAGGTGACAGTGTCTGTCTATGATGAAGATGGGAAGCGATTGGAGCACGTGATTTT CCCCGGTGCTGGCGACGAGGCGATCTCCGAGTACAAATCGGTGATTTATTATCAAGTGAAGCAGCCTCGC TGGTTTGAGACCCTTAAGGTGGCCATCCCCATTGAGGACGTGAACCGGAGTCACCTTCGATTCACCTTCC GCCATAGATCCTCGCAGGACTCCAAGGATAAATCTGAAAAGATCTTCGCACTGGCCTTTGTGAAGCTGAT GAGATACGATGGGACCACCCTGAGAGACGGGGAGCATGACCTTATTGTCTACAAGGCTGAAGCCAAAAAG CTCGAAGATGCAGCCACATACTTGAGCCTCCCTAGTACCAAGGGGGAGTTGGAAGAAAAGGGCCACTCAG CCACAGGCAAAGGAATGCAGAGTCTTGGGAGTTGTACCATCAGCAAAGACTCTTTCCAGATCTCCACTCT GGTGTGTTCCACGAAACTGACCCAGAACGTGGACCTCCTGGGGCTGTTGAAGTGGAGATCCAACACCAAC CTTCTGCAGCAGAACTTGAGGCAGTTGATGAAGGTGGACGGTGGTGAAGTGGTGAAGTTCCTTCAGGACA CCTTGGATGCCCTCTTCAACATCATGATGGAGAACTCAGAGAGTGAGACGTTTGATACCTTGGTGTTTGA TGCTCTGGTCTTTATTATTGGACTAATCGCGGATAGGAAATTTCAGCATTTTAATCCTGTTTTGGAGACT TACATTAAGAAACACTTCAGTGCAACATTAGCCTACACGAAGCTGACCAAGGTGTTACGGACCTACGTGG CCAGCGCAGAGAAGCCGGGAGTCAACGAGCAGCTGTACAAAGCCATAAAAGCCTTGGAGTACATCTTCAA GTTCATAGTGCGCTCACGGGTACTGTTCAACCAGCTGTATGAAAACAAGGGGGAAGCTGACTTCGTGGAG TCCTTGTTGCAGCTGTTCCGGTCCATCAACGACATGATGAGCAGCCTGTCAGAGCTCACAGTCAGGGTGA AGGGGGCAGCCCTGAAGTACTTGCCGACGATCGTCAATGATGTGAAGTTGGTGTTTGATCCCAAGGAGCT CAGCAAAATGTTCACGGAATTCATCCTGAATGTCCCTGCGGGCTTGCTGACGGTCCAGAAGCTCTCCTGC CTGATTGAGATTGTTCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTCCCCATGATGA CAGACCAGCTCAAGTACCACCTGGAGAGGCAGGAGGAGCTGGAGGCTTGTTGCCAGCTGCTTAGCAACAT CCTGGAGGTGCTATACCGGAAGGATGTGGGACCGACTCAGAGGCACGTCCAGATCATCATGGAGACGCTG CTTAGGACTGTGAACAGGACGGTCATTTCCATGGGGCGGGACTCCGAGCTCATTGGGAACTTTGTGGCTT GCATGACAGCCATTTTACGGCAGATGGAAGACTACCATTATGCACACCTGATCAAGACCTTTGGGAAAAT GAGGACAGATGTCGTGGATTTTCTGATGGAGACATTCATCATGTTTAAGAACCTCATCGGGAAGAACGTC TACCCCTTCGACTGGGTGATAATGAACACGATGCAGAACAAAGTCTTTCTGCGGGCGATTAATCAGTATG CAGATATGCTGAACAAGAGATTTCTGGATCAAGCCAACTTTGAGCTGCAGCTGTGGAACAACTACTTTCA CCTGGCCGTGGCTTTCCTCACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCTAAGAGAGCTAAA ATCCTTAACAAGTATGGAGACATGAGGAGGCAGATTGGCTTTGAGATCAGAGACATGTGGTACAACCTGG GTCAACACAAGATAAAATTCATCCCAGAAATGGTTGGCCCAATATTGGAAATGACATTGATTCCTGAGAC AGAGCTTCGAAAAGCCACCCTTCCCATCTTCTTCGACATGATGCAGTGTGAATTCCACTCCACCCGGAGC TTTCAAATGTTTGAAAATGAGATCATCACTAAGCTGGATCATGAAGTGGAAGGAGGCAGAGGAGACGAGC AATACAAAGTGTTATTTGATAAAATCCTCCTGGAGCACTGCAGGAAGCATAAATACCTCGCCAAGACAGG AGAAACTTTTGTGAAGCTCGTAGTGCGGTTGATGGAGAGGCTCCTGGACTACAGGACCATCATGCATGAC GAAAACAAAGACAATCGGATGAGCTGCACCGTCAACGTGCTGAATTTCTACAAAGAAATTGAAAGAGAAG AGATGTATATAAGGTACTTGTATAAGCTCTGTGACCTGCACAAAGAGTGTGACAACTACACAGAGGCGGC CTACACTTTGCTTCTGCACGCGAAACTTCTAAAGTGGTCAGAGGACGTGTGTGCAGCTCACCTCACTCAG CGGGACGGGTTCCAGGCGACAACACAGGGACAGCTGAAAGAGCAGCTCTACCAAGAAATCATCCACTACT TTGACAAAGGCAAGATGTGGGAGGAGGCCATCGCCCTGGGCAAGGAACTTGCGGAGCAGTACGAAACAGA GATGTTTGATTATGAGCAGCTCAGTGAATTGCTGAAAAAACAGGCACAATTTTACGAGAACATCGTGAAG GTGATAAGACCCAAGCCTGACTATTTTGCTGTTGGCTACTATGGGCAAGGGTTCCCGTCATTCCTTCGGG GCAAAGTGTTCATTTACCGAGGCAAGGAATATGAGCGACGGGAAGATTTTGAAGCTCGGCTGTTAACTCA GTTTCCAAATGCTGAAAAAATGAAGACGACGTCTCCACCGGGCGATGACATTAAGACTTCTCCCGGCCAG TACATCCAGTGCTTCACAGTGAAGCCCAAGCTAGACTTACCTCCGAGATTTCACCGACCCGTGTCTGAAC AGATTGTAAGTTTTTACAGGGTGAATGAGGTACAGCGATTCGAGTATTCACGGCCCATCCGGAAAGGAGA GAAAAACCCAGACAATGAATTTGCGAATATGTGGATTGAAAGAACCATCTACACTACGGCCTATAAACTG CCAGGAATTTTAAGGTGGTTTGAAGTCAAATCTGTCTTCATGGTAGAGATCAGCCCACTGGAGAATGCCA TCGAGACAATGCAGTTGACCAATGACAAGATCAGCAGCATGGTCCAGCAGCACCTGGATGACCCGGGCCT GCCTATCAACCCCCTGTCCATGCTCCTGAATGGCATTGTGGATCCTGCTGTCATGGGTGGTTTTGCCAAT TATGAGAAGGCTTTCTTCACAGACAGATACCTGCAGGAGCATCCTGAGGCCCATGGGCAGATTGAGAAGC TCAAGGACCTGATAGCCTGGCAGATTCCATTTTTGGCTGAAGGGATCAGGATTCATGGAGACAAAGTCAC AGAGGCCCTGAGGCCATTCCACGAGCGCATGGAAGCCTGCTTCAAGCAACTGAAGGAAAAAGTGGAAAAG CAATATGGCGTCAGGACCATGCCCTCAGGCTTGGATGACAGGAGAGGCAGCCGTCCACGTTCAATGGTGC GATCTTTCACCATGCCATCATCCTCCCGGCCCCTGTCAGTGGCTTCCGTCTCTTCTTTCTCCTCTGACAG CACTCCTTCCAGGCCAGGCTCTGATGGATTTGCTCTAGAGCCTCTGCTGCCGAAGAAGATGCACTCCAGG TCCCAGGACAAGCTAGACAAGGACGATCCAGATAAAGAGAAGAAGGACAAGAAGAAGGAGAAAAGGAACA GCAAACACCAAGAGATATTTGATAAGGAATTCAAGCCCGCTGACAGCTCCCTGCAGCAGTCTGAGGCTGT GATCCTTTCTGAAACGATAAGTCCCCTGCGGCCCCAAAGACCCAAGAGCCAGGTGATCAATGTCATTGGA AATGAAAGGCGCTTCTCTGTGTCTCCAGCATCACCGTCCTCCCAGCAGACGCCCCCTCCAGTCACACCAA GAGCCAAGCTCAGCTTCAGCATTCAGCCCAGTCTGGAGCTGAACGGCATGATGGGAATGGATGTGGCCGA TGTCCCACCCCCTCTGCCCCTCAAAGGCAACATGGCAGATTATGGAAATCTGATGGAAAACCAGGATATG ATGGTCTCACCCACATCTCCACCTCCTCCTCCCCCACAACGGCAACAGCCACCTCCACTGCCCAGCAAAA CACCACCTCCTCCCCCTCCGAAGACGACCCGAAAGCAGACCTCAGTGGACTCTGGAATCGTGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001033420
Insert Size	5598 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001033420.2, NP_001028592.1
RefSeq Size	6815 bp
RefSeq ORF	5598 bp
Locus ID	330662
UniProt ID	Q8BUR4
Gene Summary	Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.[UniProtKB/Swiss-Prot Function]

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