Tns1 (NM_027884) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110018I21Rik; 1200014E20Rik; AI648117; E030018G17Rik; E030037J05Rik; Tns
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225004 representing NM_027884 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCACCGTGAGCCTGGTGTGCTGCGAGGCGCTCGAGCCTCTGCCCTCCTGCGGCCCGCAGCCCC CCGGGACTCCGCCGGGCCCCGCGCGGCCCGAGCGCTGTGAGCCCGGGGGTGCGGCGCCCGACCCGAGGAG GCGGCTGCTGCTTCAGCCAGAAGACCTGGAGGCCCCCAAGACCCACCACTTCAAGGTGAAGGCCTTCAAG AAGGTGAAGCCCTGTGGCATCTGCCGGCAGGCCATCACTCGGGAAGGCTGTGTTTGCAAAGTCTGCAGCT TCTCCTGTCATCGGAAATGCCAGGCCAAGGTGGCTGCCCCTTGCGTTCCTCCGTCCAGCCATGAACTGGT GCCCATCACCACAGAGACCGTACCCAAGAATGTAGTAGATGTGGGGGAAGGAGACTGCCGGGTTGGAAGT TCTCCGAAAAACCTGGAGGAGGGCGGCTCCATGAGAGTTTCTCCAAGCATACAGCCACAACCACAATCAC AGCCTACCAGTCTCTCTAGAAACACGAGTGTGAGCCGAGCCATGGAAGACAGTTGTGAGCTGGACCTGGT GTATGTCACCGAACGCATCATCGCCGTGTCCTTCCCTAGCACAGCCAATGAGGAGAACTTCCGCAGCAAC CTCCGTGAAGTGGCTCAGATGTTGAAGTCCAAGCATGGGGGCAACTACCTGCTATTCAACCTCTCTGAAC AGAGGCCTGACATCACCAAGCTGCATGCTAAGGTCCTGGAATTCGGCTGGCCTGACCTGCACACACCGGC CCTGGAGAAGATATGCAGTGTCTGTAAGGCCATGGACACCTGGCTCAATGCAGACCCCCACAATGTCGTT GTTCTCCACAACAAGGGAAACCGAGGCAGGATTGGGGTTGTCATTGCAGCGTATCTGCACTACAGCAACA TTTCAGCCAGTGCTGACCAGGCTCTGGACCGGTTTGCAATGAAGAGATTCTATGAGGACAAGATCGTGCC CATTGGCCAGCCATCCCAGAGGAGATATGTGCACTACTTCAGTGGCCTACTCTCTGGCTCCATCAAAATG AACAACAAGCCTTTGTTTCTGCATCATGTGATCATGCACGGCATCCCCAACTTTGAGTCTAAAGGAGGGT GCCGGCCCTTTCTCCGCATCTACCAAGCCATGCAACCTGTGTACACATCTGGCATTTACAACATCCCTGG AGACAGCCAGGCCAGCATCTGTATCACCATTGAACCAGGGCTGCTCTTGAAGGGAGACATCTTGCTGAAG TGTTATCATAAGAAATTCCGGAGCCCGGCTCGAGACGTCATCTTCCGAGTGCAGTTCCACACCTGTGCCA TCCATGACTTGGGGGTTGTTTTTGGGAAGGAAGATCTGGATGAGGCCTTCAAAGATGATCGATTTCCTGA CTATGGCAAAGTGGAGTTTGTCTTTTCCTATGGACCAGAGAAAATTCAAGGCATGGAACACCTGGAGAAT GGGCCCAGCGTATCTGTAGACTATAACACTTCTGACCCCCTCATCCGCTGGGACTCCTATGACAACTTCA GTGGGCATCGTGAGGATGGCATGGAAGAAGTGGTGGGACACACCCAAGGGCCGCTGGATGGGAGCCTGTA CGCCAAGGTGAAGAAGAAGGATTCCCTGAATGGCAGTTCGGGGCCTGTCACTACTGCACGGCCTGCCCTG TCAGCCACTCCCAACCACGTGGAACACACCCTGTCTGTGAGCAGCGACTCTGGCAACTCCACAGCCTCCA CTAAGACAGACAAGACCGATGAGCCAGTCTCTGGGGCTACCACTGCCCCTGCTGCCCTGAGTCCTCAGGA GAAGAAAGAGCTGGACCGCCTACTAAGTGGCTTTGGTGTGGATAGAGAAAAGCAAGGCGCCATGTACCGG GCTCAGCAACTCAGATCCCATCCAGGCGGGGGCCCGACTGTGCCCTCCCCTGGCCGCCACATTGTCCCAG CCCAGGTTCACGTCAATGGTGGGGCCCTGGCATCTGAGCGGGAGACAGACATTCTAGATGATGAGCTGCC CATCCAGGACGGGCAGAGTGGGGGCAGCATGGGAACGCTGTCCTCCCTGGATGGGGTCACCAACACCAGT GAAAGTGGCTACCCAGAGACCCTGTCCCCACTGACCAATGGCCTGGACAAACCCTATTCCACAGAACCTG TGCTCAACGGCGGAGGCTACCCCTATGAGGCTGCCAACCGGGTGATTCCTGTCCACAGCAGTCACTCGGC CCCCATTCGACCCTCCTACTCTGCTCAGGAGGGTTTAGCTGGCTACCAGCGTGAGGGACCCCACCCAGCC TGGTCACAGCAAGTGACCAGTGCCCACTGTGGCTGTGACCCCAGTGGTCTATTCAGATCCCAGTCCTTTC CAGATGTGGAGCCCCAGCTGCCCCAAGCTCCAACCCGTGGGGGTAGTAGCCGAGAGGCTGTGCAGAGGGG TCTGAATTCATGGCAACAGCAGCAGCCCCACCCACCTCCCCGCCAGCAGGAGAGATCCCCACTGCAGAGT CTTGCCCGCAGCAAGCCTAGCCCCCAGCTGTCAGCAGAGACCCCCGTAGCTGCTCTCCCAGAGTTCCCAA GGGCAGCGTCCCAGCAGGAGATCGAGCAGTCCATCGAAACCCTCAATATGTTGATGCTGGACCTGGAGCC AGCCTCCGCAGCTGCCCCTCTTCATAAGTCCCAGAGTGTCCCTGGGGCCTGGCCAGGGGCTTCTCCACTC TCTTCCCAGCCCCTCTTGGGGTCTTCCCGCCAGTCCCACCCACTTACCCAGTCCCGATCTGGCTATATCC CCAGTGGGCATTCTCTGGGAACCCCGGAGCTGGTCTCTTCTGGCAGGCCTTATTCACCTTACGATTATCA GCTGCATCCAGCTGGATCCAACCAGAGTTTCCATCCAAAAAGTCCAGCCTCTTCCACCTTCCTTCCAAGC CCTCATAGCTCTGCGGGGCCTCAGGAGCCCCCAGCCTCTCTCCCTGGTCTCATCGCTCAGCCCCAGCTCC CACCAAAGGAAACTACATCAGATCCCTCCCGAACTCCAGAGGAAGAGCCGCTGAACCTGGAAGGGCTGGT AGCCCATCGGGTAGCAGGGGTTCAGGCTCGGGAGAGGCAGCCTGCAGAGCCCCCAGGCCCACTCCGGAGG CGGGCAGCCAGTGATGGACAGTATGAAAACCAGTCTCCGGAGGCCACATCCCCCCGTAGTCCTGGGGTCC GCTCCCCCGTCCAGTGCGTCTCCCCTGAGCTGGCTCTTACCATTGCCCTCAATCCTGGAGGGCGCCCCAA AGAGCCTCATTTGCATAGCTACAAGGAGGCCTTTGAGGAGATGGAGGGGACCTCCCCAAGCAGCCCGCCA CACAGTGTGGCGCGCTCTCCTCCAGGTCTGGCCAAGACACCCCTGTCTGCTCTGGGCCTGAAACCCCACA ACCCAGCAGACATCTTGCTGCACCCCACAGGTGTTGCCAGAAGACTGATCCAGCCAGAGGAAGACGAGGG GGAGGAGGTGACTAAGCCTCCAGAAGAGCCCCGGAGCTATGTGGAGTCCGTGGCGAGGACAGCGGTGGCA GGGCCTCGAGCTCAGGACGTTGAGCCTAAGAGCTTCAGCGCACCAGCTGCTCACGCCTATGGGCACGAGA CACCCCTGAGGAACGGGACCCCAGGGGGCTCCTTTGTCTCCCCGAGCCCCCTCTCCACAAGCAGCCCCAT CCTCAGTGCTGACAGCACTTCCGTGGGCAGTTTCCCATCAGTGGTGAGCAGTGACCAGGGTCCCCGGACA CCCTTCCAGCCTATGCTGGACTCCAGCATCCGCTCAGGCAGCTTGGGGCAGCCGAGCCCTGCAGCACTGA GTTATCAGAGCTCCTCGCCTGTCCCTGTTGGTGGCAGCAGCTACAACAGCCCTGACTACTCCCTCCAACC ATTCAGCTCTTCTCCAGAAAGCCAAGGCCAGCCACAGTACAGTGCGGCCAGTGTCCACATGGTACCTGGG AGCCCTCAGGCACGACACAGGACAGTGGGTACCAACACTCCACCTAGCCCTGGTTTTGGCCGGCGGGCCG TCAACCCCACCATGGCTGCCCCTGGTAGTCCCAGTTTGAGCCATCGGCAGGTGATGGGTCCATCTGGTCC AGGTTTCCATGGTAACGTGGTTTCTGGCCACCCAGCCAGTGCAGCTACCACTCCTGGAAGCCCCAGCCTG GGCCGGCACCCAGTGGGAAGCCATCAAGTTCCAGGCCTCCACAGCAGTGTGGTCACCACTCCGGGAAGCC CAAGCCTGGGCCGACACCCTGGTGCCCACCAAGGGAATCTGGCCTCCAGTCTCCACAGCAATGCAGTCAT CAGCCCTGGGAGCCCAAGCTTGGGCCGGCACCTGGGTGGGTCTGGATCCGTGGTTCCTGGAAGCCCCAGC TTGGACCGGCATGCAGCTTATGGTGGCTACTCTACCCCTGAGGACCGAAGACCAACACTGTCCCGGCAAA GCAGTGCCTCTGGCTACCAGGCCCCATCCACACCGTCATTCCCTGTCTCTCCTGCCTACTACCCTGGCCT GAGCAGCCCTGCCACCTCACCCTCGCCAGACTCAGCAGCCTTCCGGCAGGGGAGCCCCACACCAGCCTTA CCAGAGAAGCGAAGAATGTCAGTGGGAGACCGGGCAGGCAGCCTCCCCAACTATGCCACCATCAATGGGA AAGTGTCTTCCTCGCCTGTCGCCAATGGCATGGCCAGTGGGAGCAGCACCGTCTCCTTCTCTCACACGCT TCCGGACTTTTCTAAGTATTCGATGCCAGACAATAGTCCGGAGACTCGGGCTAAAGTGAAGTTTGTCCAG GACACTTCCAAGTATTGGTACAAGCCTGAGATCTCCAGAGAACAGGCCATCGCACTCCTAAAGGACCAGG AGCCAGGAGCCTTCATCATCCGGGACAGCCACTCCTTCCGAGGGGCTTATGGGCTGGCCATGAAGGTGTC CTCACCCCCTCCAACCATCACTCAGCAGGGCAAGAAAGGAGACATGACCCATGAGCTGGTGAGGCACTTT CTGATCGAGACAGGCCCTAGAGGAGTGAAGCTCAAGGGTTGTCCCAATGAGCCAAACTTTGGTTCCCTTT CTGCCCTGGTCTATCAGCACTCCGTCATCCCACTGGCCCTGCCCTGTAAACTGGTCATTCCAAGCCGAGA CCCCACAGATGAATCAAAAGATAGCTCAGGCCCTGCCAACTCAACCACTGACCTGCTGAAGCAAGGAGCA GCTTGCAATGTGCTCTTCGTCAACTCTGTGGATATGGAGTCACTCACCGGGCCACAGGCCATTTCCAAAG CCACATCTGAGACTCTGGCTGCCGACCCCACACCGGCTGCCACCATTGTTCACTTCAAGGTCTCTGCCCA AGGAATCACACTGACTGACAATCAAAGAAAGCTCTTCTTTAGAAGACACTACCCCCTCAACACTGTCACC TTCTGTGACCTGGATCCACAAGAAAGAAAGTGGATGAAGACAGAGGGAGGCGCCCCTGCTAAGCTCTTTG GCTTCGTGGCCCGGAAGCAGGGCAGCACCACAGACAACGCTTGCCACCTCTTCGCAGAGCTTGACCCCAA CCAGCCTGCGTCTGCCATCGTCAACTTCGTCTCCAAGGTCATGCTGAGTGCTGGCCAGAAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027884
Insert Size	5667 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027884.3, NP_082160.3
RefSeq Size	9966 bp
RefSeq ORF	5667 bp
Locus ID	21961

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