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Tns1 (NM_027884) Mouse Tagged ORF Clone
CAT＃: MG222153
- TrueORF®
Tns1 (tGFP-tagged) - Mouse tensin 1 (Tns1), (10ug)
ORF Plasmid: DDK
"NM_027884" in other vectors (2)
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CNY 22,800.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110018I21Rik; 1200014E20Rik; AI648117; E030018G17Rik; E030037J05Rik; Tns |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222153 representing NM_027884
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCACCGTGAGCCTGGTGTGCTGCGAGGCGCTCGAGCCTCTGCCCTCCTGCGGCCCGCAGCCCC CCGGGACTCCGCCGGGCCCCGCGCGGCCCGAGCGCTGTGAGCCCGGGGGTGCGGCGCCCGACCCGAGGAG GCGGCTGCTGCTTCAGCCAGAAGACCTGGAGGCCCCCAAGACCCACCACTTCAAGGTGAAGGCCTTCAAG AAGGTGAAGCCCTGTGGCATCTGCCGGCAGGCCATCACTCGGGAAGGCTGTGTTTGCAAAGTCTGCAGCT TCTCCTGTCATCGGAAATGCCAGGCCAAGGTGGCTGCCCCTTGCGTTCCTCCGTCCAGCCATGAACTGGT GCCCATCACCACAGAGACCGTACCCAAGAATGTAGTAGATGTGGGGGAAGGAGACTGCCGGGTTGGAAGT TCTCCGAAAAACCTGGAGGAGGGCGGCTCCATGAGAGTTTCTCCAAGCATACAGCCACAACCACAATCAC AGCCTACCAGTCTCTCTAGAAACACGAGTGTGAGCCGAGCCATGGAAGACAGTTGTGAGCTGGACCTGGT GTATGTCACCGAACGCATCATCGCCGTGTCCTTCCCTAGCACAGCCAATGAGGAGAACTTCCGCAGCAAC CTCCGTGAAGTGGCTCAGATGTTGAAGTCCAAGCATGGGGGCAACTACCTGCTATTCAACCTCTCTGAAC AGAGGCCTGACATCACCAAGCTGCATGCTAAGGTCCTGGAATTCGGCTGGCCTGACCTGCACACACCGGC CCTGGAGAAGATATGCAGTGTCTGTAAGGCCATGGACACCTGGCTCAATGCAGACCCCCACAATGTCGTT GTTCTCCACAACAAGGGAAACCGAGGCAGGATTGGGGTTGTCATTGCAGCGTATCTGCACTACAGCAACA TTTCAGCCAGTGCTGACCAGGCTCTGGACCGGTTTGCAATGAAGAGATTCTATGAGGACAAGATCGTGCC CATTGGCCAGCCATCCCAGAGGAGATATGTGCACTACTTCAGTGGCCTACTCTCTGGCTCCATCAAAATG AACAACAAGCCTTTGTTTCTGCATCATGTGATCATGCACGGCATCCCCAACTTTGAGTCTAAAGGAGGGT GCCGGCCCTTTCTCCGCATCTACCAAGCCATGCAACCTGTGTACACATCTGGCATTTACAACATCCCTGG AGACAGCCAGGCCAGCATCTGTATCACCATTGAACCAGGGCTGCTCTTGAAGGGAGACATCTTGCTGAAG TGTTATCATAAGAAATTCCGGAGCCCGGCTCGAGACGTCATCTTCCGAGTGCAGTTCCACACCTGTGCCA TCCATGACTTGGGGGTTGTTTTTGGGAAGGAAGATCTGGATGAGGCCTTCAAAGATGATCGATTTCCTGA CTATGGCAAAGTGGAGTTTGTCTTTTCCTATGGACCAGAGAAAATTCAAGGCATGGAACACCTGGAGAAT GGGCCCAGCGTATCTGTAGACTATAACACTTCTGACCCCCTCATCCGCTGGGACTCCTATGACAACTTCA GTGGGCATCGTGAGGATGGCATGGAAGAAGTGGTGGGACACACCCAAGGGCCGCTGGATGGGAGCCTGTA CGCCAAGGTGAAGAAGAAGGATTCCCTGAATGGCAGTTCGGGGCCTGTCACTACTGCACGGCCTGCCCTG TCAGCCACTCCCAACCACGTGGAACACACCCTGTCTGTGAGCAGCGACTCTGGCAACTCCACAGCCTCCA CTAAGACAGACAAGACCGATGAGCCAGTCTCTGGGGCTACCACTGCCCCTGCTGCCCTGAGTCCTCAGGA GAAGAAAGAGCTGGACCGCCTACTAAGTGGCTTTGGTGTGGATAGAGAAAAGCAAGGCGCCATGTACCGG GCTCAGCAACTCAGATCCCATCCAGGCGGGGGCCCGACTGTGCCCTCCCCTGGCCGCCACATTGTCCCAG CCCAGGTTCACGTCAATGGTGGGGCCCTGGCATCTGAGCGGGAGACAGACATTCTAGATGATGAGCTGCC CATCCAGGACGGGCAGAGTGGGGGCAGCATGGGAACGCTGTCCTCCCTGGATGGGGTCACCAACACCAGT GAAAGTGGCTACCCAGAGACCCTGTCCCCACTGACCAATGGCCTGGACAAACCCTATTCCACAGAACCTG TGCTCAACGGCGGAGGCTACCCCTATGAGGCTGCCAACCGGGTGATTCCTGTCCACAGCAGTCACTCGGC CCCCATTCGACCCTCCTACTCTGCTCAGGAGGGTTTAGCTGGCTACCAGCGTGAGGGACCCCACCCAGCC TGGTCACAGCAAGTGACCAGTGCCCACTGTGGCTGTGACCCCAGTGGTCTATTCAGATCCCAGTCCTTTC CAGATGTGGAGCCCCAGCTGCCCCAAGCTCCAACCCGTGGGGGTAGTAGCCGAGAGGCTGTGCAGAGGGG TCTGAATTCATGGCAACAGCAGCAGCCCCACCCACCTCCCCGCCAGCAGGAGAGATCCCCACTGCAGAGT CTTGCCCGCAGCAAGCCTAGCCCCCAGCTGTCAGCAGAGACCCCCGTAGCTGCTCTCCCAGAGTTCCCAA GGGCAGCGTCCCAGCAGGAGATCGAGCAGTCCATCGAAACCCTCAATATGTTGATGCTGGACCTGGAGCC AGCCTCCGCAGCTGCCCCTCTTCATAAGTCCCAGAGTGTCCCTGGGGCCTGGCCAGGGGCTTCTCCACTC TCTTCCCAGCCCCTCTTGGGGTCTTCCCGCCAGTCCCACCCACTTACCCAGTCCCGATCTGGCTATATCC CCAGTGGGCATTCTCTGGGAACCCCGGAGCTGGTCTCTTCTGGCAGGCCTTATTCACCTTACGATTATCA GCTGCATCCAGCTGGATCCAACCAGAGTTTCCATCCAAAAAGTCCAGCCTCTTCCACCTTCCTTCCAAGC CCTCATAGCTCTGCGGGGCCTCAGGAGCCCCCAGCCTCTCTCCCTGGTCTCATCGCTCAGCCCCAGCTCC CACCAAAGGAAACTACATCAGATCCCTCCCGAACTCCAGAGGAAGAGCCGCTGAACCTGGAAGGGCTGGT AGCCCATCGGGTAGCAGGGGTTCAGGCTCGGGAGAGGCAGCCTGCAGAGCCCCCAGGCCCACTCCGGAGG CGGGCAGCCAGTGATGGACAGTATGAAAACCAGTCTCCGGAGGCCACATCCCCCCGTAGTCCTGGGGTCC GCTCCCCCGTCCAGTGCGTCTCCCCTGAGCTGGCTCTTACCATTGCCCTCAATCCTGGAGGGCGCCCCAA AGAGCCTCATTTGCATAGCTACAAGGAGGCCTTTGAGGAGATGGAGGGGACCTCCCCAAGCAGCCCGCCA CACAGTGTGGCGCGCTCTCCTCCAGGTCTGGCCAAGACACCCCTGTCTGCTCTGGGCCTGAAACCCCACA ACCCAGCAGACATCTTGCTGCACCCCACAGGTGTTGCCAGAAGACTGATCCAGCCAGAGGAAGACGAGGG GGAGGAGGTGACTAAGCCTCCAGAAGAGCCCCGGAGCTATGTGGAGTCCGTGGCGAGGACAGCGGTGGCA GGGCCTCGAGCTCAGGACGTTGAGCCTAAGAGCTTCAGCGCACCAGCTGCTCACGCCTATGGGCACGAGA CACCCCTGAGGAACGGGACCCCAGGGGGCTCCTTTGTCTCCCCGAGCCCCCTCTCCACAAGCAGCCCCAT CCTCAGTGCTGACAGCACTTCCGTGGGCAGTTTCCCATCAGTGGTGAGCAGTGACCAGGGTCCCCGGACA CCCTTCCAGCCTATGCTGGACTCCAGCATCCGCTCAGGCAGCTTGGGGCAGCCGAGCCCTGCAGCACTGA GTTATCAGAGCTCCTCGCCTGTCCCTGTTGGTGGCAGCAGCTACAACAGCCCTGACTACTCCCTCCAACC ATTCAGCTCTTCTCCAGAAAGCCAAGGCCAGCCACAGTACAGTGCGGCCAGTGTCCACATGGTACCTGGG AGCCCTCAGGCACGACACAGGACAGTGGGTACCAACACTCCACCTAGCCCTGGTTTTGGCCGGCGGGCCG TCAACCCCACCATGGCTGCCCCTGGTAGTCCCAGTTTGAGCCATCGGCAGGTGATGGGTCCATCTGGTCC AGGTTTCCATGGTAACGTGGTTTCTGGCCACCCAGCCAGTGCAGCTACCACTCCTGGAAGCCCCAGCCTG GGCCGGCACCCAGTGGGAAGCCATCAAGTTCCAGGCCTCCACAGCAGTGTGGTCACCACTCCGGGAAGCC CAAGCCTGGGCCGACACCCTGGTGCCCACCAAGGGAATCTGGCCTCCAGTCTCCACAGCAATGCAGTCAT CAGCCCTGGGAGCCCAAGCTTGGGCCGGCACCTGGGTGGGTCTGGATCCGTGGTTCCTGGAAGCCCCAGC TTGGACCGGCATGCAGCTTATGGTGGCTACTCTACCCCTGAGGACCGAAGACCAACACTGTCCCGGCAAA GCAGTGCCTCTGGCTACCAGGCCCCATCCACACCGTCATTCCCTGTCTCTCCTGCCTACTACCCTGGCCT GAGCAGCCCTGCCACCTCACCCTCGCCAGACTCAGCAGCCTTCCGGCAGGGGAGCCCCACACCAGCCTTA CCAGAGAAGCGAAGAATGTCAGTGGGAGACCGGGCAGGCAGCCTCCCCAACTATGCCACCATCAATGGGA AAGTGTCTTCCTCGCCTGTCGCCAATGGCATGGCCAGTGGGAGCAGCACCGTCTCCTTCTCTCACACGCT TCCGGACTTTTCTAAGTATTCGATGCCAGACAATAGTCCGGAGACTCGGGCTAAAGTGAAGTTTGTCCAG GACACTTCCAAGTATTGGTACAAGCCTGAGATCTCCAGAGAACAGGCCATCGCACTCCTAAAGGACCAGG AGCCAGGAGCCTTCATCATCCGGGACAGCCACTCCTTCCGAGGGGCTTATGGGCTGGCCATGAAGGTGTC CTCACCCCCTCCAACCATCACTCAGCAGGGCAAGAAAGGAGACATGACCCATGAGCTGGTGAGGCACTTT CTGATCGAGACAGGCCCTAGAGGAGTGAAGCTCAAGGGTTGTCCCAATGAGCCAAACTTTGGTTCCCTTT CTGCCCTGGTCTATCAGCACTCCGTCATCCCACTGGCCCTGCCCTGTAAACTGGTCATTCCAAGCCGAGA CCCCACAGATGAATCAAAAGATAGCTCAGGCCCTGCCAACTCAACCACTGACCTGCTGAAGCAAGGAGCA GCTTGCAATGTGCTCTTCGTCAACTCTGTGGATATGGAGTCACTCACCGGGCCACAGGCCATTTCCAAAG CCACATCTGAGACTCTGGCTGCCGACCCCACACCGGCTGCCACCATTGTTCACTTCAAGGTCTCTGCCCA AGGAATCACACTGACTGACAATCAAAGAAAGCTCTTCTTTAGAAGACACTACCCCCTCAACACTGTCACC TTCTGTGACCTGGATCCACAAGAAAGAAAGTGGATGAAGACAGAGGGAGGCGCCCCTGCTAAGCTCTTTG GCTTCGTGGCCCGGAAGCAGGGCAGCACCACAGACAACGCTTGCCACCTCTTCGCAGAGCTTGACCCCAA CCAGCCTGCGTCTGCCATCGTCAACTTCGTCTCCAAGGTCATGCTGAGTGCTGGCCAGAAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222153 representing NM_027884
Red=Cloning site Green=Tags(s) MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFK KVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGS SPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSN LREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVV VLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKM NNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLK CYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLEN GPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPAL SATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYR AQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTS ESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPA WSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQS LARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPL SSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPS PHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRR RAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPP HSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVA GPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRT PFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPG SPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSL GRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPS LDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPAL PEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQ DTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHF LIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGA ACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVT FCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027884 |
| ORF Size | 5664 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027884.4 |
| RefSeq Size | 9966 bp |
| RefSeq ORF | 5667 bp |
| Locus ID | 21961 |
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