Sbf1 (NM_001170561) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610510A08Rik; B230113C15Rik; mKIAA3020; Mtmr5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225014 representing NM_001170561 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGCTCGCGGACTACTTCGTGCTGGTGGCGTTCGGGCCGCACCCGCGCGGGAGTGGGGAAGGCC AGGGCCAGATCCTGCAGCGCTTCCCGGAGAAGGACTGGGAGGACAACCCTTTCCCCCAGGGCATTGAGCT GTTTTGCCAGCCCAGTGGGTGGCAGCTATGTCCCGAGAGGAATCCACCAACTTTCTTTGTGGCTGTCCTT ACTGACATCAACTCTGAGAGGCACTACTGCGCCTGCTTGACCTTCTGGGAGCCAGTGGAGTCCACACAGG AAGTAGTGTGCACTGACAATGCCACGGAGAAGGAGGAAGAGGCAGATGGAGGAGGCCAAGCGCGGCTGTC ATCCACAGCCCCAGCCCAGCCTGGCCAGCTTTTTGCGCCAAAGACTCTGGTTCTGGTGTCTCGACTGGAC CATGCCGAAGTGTTCAGGAATAGCCTTGGTCTCATCTATGCTATCCACGTGGAAGGCCTGAATGTGAGCC TCGAGAATGTGATCGGGAACCTGCTCACATGTACCGTCCCATTGGCTGGCGGGTCTCAGAGAACCATCTC TTTGGGGGCTGGTGACCGGCAGGTCATTCAGACTCCACTGGTAGACTCACTGCCTGTCAGCCGCTGTAGT GTAGCCCTGCTCTTCCGCCAGCTGGGTATCACCAATGTGCTGTCTTTGTTCTGTGCTGCCCTTACTGAGC ACAAAGTCCTCTTCTTGTCCAGGAGCTACCAACGTCTAGCAGATGCTTGCAGGGGTCTCTTGGCATTGCT GTTCCCTCTCAGATACAGCTTCACTTATGTGCCCATCTTGCCGGCACAGCTGCTGGAAGTCCTTAGCACA CCTACGCCTTTCATCATTGGGGTCAATGCAGCCTTCCAGGCAGAGACTCAGGAGCTGCTGGATGTGATTG TTGCTGATCTTGACGGAGGGACAGTGACTGTCCCTGAGTGTGTGCACATTCCACCCCTGCCAGAGCCACT ACAAAGCCAGACCCACAATGTTCTGAGCATGGTCCTGGATCCAGAACTGGAGTTGGCTGACCTTGCCTTC CCACCCCCTACAACATCTGCTTCCTCCCTGAAAATGCAGGACAAGGAGCTCCGTGCTGTCTTTCTGCGGC TCTTTGCTCAGCTCCTGCAAGGCTACCGCTGGTGCCTGCACATCGTGCGCATCCACCCAGAGCCCGTCAT CCGCTTCCATAAGGCAGCATTCTTAGGCCAGCGTGGACTGGTGGAGGATGATTTCCTGATGAAAGTATTG GAGGGCATGGCCTTCGCGGGCTTTGTATCTGAGCGTGGGGTTCCCTACCGTGCCACAGACCTGTTTGATG AGCTGGTGGCTCATGAGGTAGCACGGATGCGAGCAGATGAGAGCCATCCCCACCGTGTCCTGCGTCATGT CCAGGAACTGGCAGAGCAACTTTATAAGAATGAAAACCCGTACCCAGCTGTGGCGATGCACAAAGTGCAG AGGCCAGGAGAGGCCAGTCACCTGCGGCGGACCCACCGGCCATTCCCCCGGCTAGATGAGGGCACAATTC AGTGGATTGTGGACCAGGCTGCGGCCAAGATGCAGGGCGCACCTCCAGCTGTCAAGGCTGAGCGGAGGAG CACTGTGCCTTCAGGGCCCCCCATGACGGCCATACTGGAGCGCTGTAGTGGGCCTCATATCAACAGTGCA CGTCGCTTGGAGGTAGTACGGAATTGCATCTCCTATGTGTTTGAAGGGAAAATGCTTGAGGCAAAGAAGT TGCTTCCGGCTGTACTCAGGGCCCTGAAGGGGCGAGCTGCCCGTCGCTGCCTTGCCCATGAGCTTCACCT GCACGTACAGCAGAACCGGGCAGTTCTGGATCATCAGCAGTTTGACTTTGTCGTCCGCATGATGAATTGC TGCCTGCAGGATTGCACTTCCCTGGATGAGCATGGCATTGCATCTGCACTGCTGCCTTTGGTCACAGCCT TCTGCAGGAAGCTGAGCCCAGGGGTGACACAGTTTGCATACAGCTGTGTTCAGGAGCACGTAGTGTGGAG CACACCTCAGTTCTGGGAGGCCATGTTCTATGGGGATGTGCAGACCCATATCCGAGCCCTCTACCTGGAG CCCTCTGATGGCGTGAGCCCCACCCAGGAGACTGGGGAGGCACAGTCTCAGGATGATGAACGATCTGCCC TGGATGTGGCTTCAGAGCAGAGGCGCCTGTGGCCAACCCTGAGCCGTGAGAAGCAGCAGGAGCTGGTACA GAAGGAGGAAAGCACTGTGTTCAGCCAGGCCATCCACTATGCCAACCGCATGAGCTACCTTCTGCTGCCT CTGGACAGCAGCAAGAGCCGGCTGCTGCGGGAGCGGGCAGGGTTGGGAGACCTGGAGAGTGCCAGCAACA GCCTGGTCACCAACAGCATGGCAGGCAGTGTGGCTGAGAGCTATGACACAGAGAGTGGCTTTGAAGATGC AGAGACGTGCGATGTGGCTGGGGCTGTAGTCCGCTTCATCAACCGCTTTGTGGACAAAGTCTGCACAGAG AGTGGGGTCACCAGCGACCACCTCAAAGGACTGCATGTCATGGTGCCAGACATTGTCCAGATGCACATTG AGACCCTGGAGGCCGTACACCGTGAGAGCAAGAGGCTGCCCCCGATACAGAAGCCCAAGCTGCTGAGGCC ACGCCTGTTGCCTGGTGAGGAGTGTGTCCTCGATGGCCTTCGAGTGTATCTGCTGCCAGACGGGCGTGAG GAGGGTGTAGGAGGCAGTGGAGGGGGCCCTGCTCTACTCCCAGCTGAGGGTGCTGTCTTCCTTACCACAT ACCGCGTCATCTTCACGGGGATGCCTACTGACCCCCTGGTGGGGGAGCAGGTGGTAGTCCGCTCCTTCCC CGTGGCTGCCTTGACCAAGGAGAAGCGCATTAGTGTGCAGACCCCTGTGGACCAGCTTCTGCAGGATGGG CTGCAGCTTCGTTCCTGCACATTCCAGCTGCTGAAAATGGCCTTTGATGAGGAGGTGGGATCTGACAGTG CTGAGCTCTTCCGAAAGCAGCTACACAAGCTTCGGTACCCACCAGACATCAGGGCCACCTTTGCATTCAC ACTTGGCTCAGCTCACACACCTGGCAGGCCGCCACGGGTTACCAAGGACAAGGGTCCTTCATTCAGAACC CTATCCCGGAACCTGATGAAGAATGCTAAAAAGACCATTGGGCGGCAGTATGTTACTCGTAAGAAGTATA ACCCCCCTGGCTGGGAGCATCGGGGCCAGCCACCCCCTGAGGACCAGGAGGACGAGATCTCAGTGTCAGA GGAGCTGGAGCCTAGCACACTGACCCCGTCCTCAGCCCTGAAGCCCTCTGACCGCATGACCATGAGCAGC CTGGTGGAACGGGCATGTTGCCGTGACTACCAGCGCCTTGGACTAGGTACCCTGAGCAGCAGCCTGAGCC GGGCCAAGTCTGAGCCCTTTCGCATCTCTCCTGTTAATCGCATGTATGCCATATGTCGCAGCTATCCAGG ATTGCTGATTGTTCCCCAGAGCATCCAGGACAACGCCCTGCAGCGTGTATCCCGCTGCTACCGCCAGAAC CGCTTCCCTGTGGTCTGCTGGCGCAGTGGGCGCTCCAAGGCTGTCTTGCTACGCTCTGGGGGCCTGCATG GCAAAGGTGTTGTTGGTCTCTTCAAGGCCCAGAATACACCTTCTCCAGGCCAGGCCCAGGCCGACTCCAG CAGCCTGGAGCAAGAGAAGTACCTGCAGGCTGTGGTCAGCTCCATGCCACGTTATGCTGACTCATCAGGA CGAAACACACTGAGTAGCTTCTCCTCAGCCCACATGGGTGGCCATGTGCCCAGCCCCCGAGCCAGGGTCA CCACGCTGTCCAACCCTTTGGCGGCCTCGGCCTCCAGATGGACTGCGTCCCGAGGGAAATGGAGCAGTGT CCGAGCCAGTGGTCGAAGCAGTGGTCTTGGTTCTGATGTGGGCTCTCGGCTAGCTGGCAGAGATCTCCTC AGTACTCCCCACACCAACGGAGCTCCACCTGATTCTGGTTTTCTACGGCCACAGCGTGCAGCCCTCTACA TCATTGGTGACAAAGCTCAGCTCAAGGGTGTGCGCCCAGATCCCTTGCAACAGTGGGAGCTGGTACCTAT TGAAGTATTTGAGGCAAGGCAGGTGAAAGCCAGCTTCAAGAAACTATTGAAAGCCTGTGTGCCTGGTTGC CCTGCCACTGAGCCCAGCCCAGCCTCTTTTCTGCGCTCACTGGAGGACTCAGAATGGCTGATCCAGATCC ACAAGCTGCTGCAGATATCGGTGCTGGTAGTGGAGCTGCTCGACTCTGGCTCCTCTGTCCTGGTGAGCCT GGAGGACGGCTGGGACATCACCACTCAGGTGGTATCCTTGGTGCAGCTGCTCTCAGACCCCTTCTACCGC ACTCTGGAAGGCTTCCGACTGCTAGTGGAAAAAGAGTGGCTGTCCTTTGGCCATCGTTTCAGCCACCGCG GGGCCCACACCCTAGCTGGGCAGAGCAGTGGCTTCACACCCGTCTTCCTGCAGTTCCTGGACTGTGTACA CCAGGTCCATTTGCAGTTCCCCATGGAGTTTGAGTTCAGTCAGTTCTACCTCAAGTTCCTTGGTTACCAC CACACATCCCGCCGTTTCCGGACCTTCTTGCTGGACTCTGATTATGAGCGTATTGAGCTGGGGCTGCTGT ATGAGGAGAAGGGTGAACGCAGGGGTCAGCTGGCCTGCAAGTCAGTGTGGGAGTATGTAGACCGGCTAAG CAAGAGGACCCCCATGTTCTACAACTACACATATGCACCCGAGGACACAGAGGTGCTGCGACCCTACAGC AATGTGTCCAATCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGGGGCCCCCTTATGACT GGGAGCTGGCCCAGGGACCCCCTGAGCCTCCAGAAGAGGAACGTCCTGATGGAGGTGCTCCCCAGAGCAG GCGCCGTGTGGTATGGCCATGCTATGACAGCCGTCCTCGAGTCCAGCCTGATGCCATCTCACGTCTGCTG GAGGAGCTGCAGCGCTTGGAGACAGAGTTAGGTCGGCCTTCTGAACGCTGGAAAGACACCTGGGACCGGG TGAAGGCTGCTCAGCGCCTTGAAAGCCGGCAAGATGGACGTGGTACCCCCAGCTCACTTCTGGTGTCGGC TGTGCCCCACCACCGCCGCTCGCTAGGCGTCTATCTACAGGAGGGGCCTGTGGGCTCTACTCTTAGCCTC AGCCTGGACAGTGACCAGAGCAGTGGCTCAACCACATCTAGCTCTCGGCAGGCCGCCCGGCGGAGCACCA GCACCCTGTACAGCCAGTTCCAGACAGCTGAGAGTGAGAACAGGTCCTATGAGGGCATCCTATACAAGAA GGGGGCCTTCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTAGACAAGACCAAGCACCAGCTGCGATAC TATGACCACCGAATGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGGAAGCTGTGGCACCTG GCACACCCACTATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTTGATGTGAAGACAACACGTCG CGTTTACAACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGGATCCAGAGCTGCCTG TCGGATGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170561
Insert Size	5682 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170561.1, NP_001164032.1
RefSeq Size	6268 bp
RefSeq ORF	5682 bp
Locus ID	77980
UniProt ID	Q6ZPE2
Gene Summary	Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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