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Sbf1 (NM_001170561) Mouse Tagged ORF Clone
CAT＃: MG217371
- TrueORF®
Sbf1 (tGFP-tagged) - Mouse SET binding factor 1 (Sbf1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001170561" in other vectors (2)
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CNY 15,580.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610510A08Rik; B230113C15Rik; mKIAA3020; Mtmr5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217371 representing NM_001170561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGCTCGCGGACTACTTCGTGCTGGTGGCGTTCGGGCCGCACCCGCGCGGGAGTGGGGAAGGCC AGGGCCAGATCCTGCAGCGCTTCCCGGAGAAGGACTGGGAGGACAACCCTTTCCCCCAGGGCATTGAGCT GTTTTGCCAGCCCAGTGGGTGGCAGCTATGTCCCGAGAGGAATCCACCAACTTTCTTTGTGGCTGTCCTT ACTGACATCAACTCTGAGAGGCACTACTGCGCCTGCTTGACCTTCTGGGAGCCAGTGGAGTCCACACAGG AAGTAGTGTGCACTGACAATGCCACGGAGAAGGAGGAAGAGGCAGATGGAGGAGGCCAAGCGCGGCTGTC ATCCACAGCCCCAGCCCAGCCTGGCCAGCTTTTTGCGCCAAAGACTCTGGTTCTGGTGTCTCGACTGGAC CATGCCGAAGTGTTCAGGAATAGCCTTGGTCTCATCTATGCTATCCACGTGGAAGGCCTGAATGTGAGCC TCGAGAATGTGATCGGGAACCTGCTCACATGTACCGTCCCATTGGCTGGCGGGTCTCAGAGAACCATCTC TTTGGGGGCTGGTGACCGGCAGGTCATTCAGACTCCACTGGTAGACTCACTGCCTGTCAGCCGCTGTAGT GTAGCCCTGCTCTTCCGCCAGCTGGGTATCACCAATGTGCTGTCTTTGTTCTGTGCTGCCCTTACTGAGC ACAAAGTCCTCTTCTTGTCCAGGAGCTACCAACGTCTAGCAGATGCTTGCAGGGGTCTCTTGGCATTGCT GTTCCCTCTCAGATACAGCTTCACTTATGTGCCCATCTTGCCGGCACAGCTGCTGGAAGTCCTTAGCACA CCTACGCCTTTCATCATTGGGGTCAATGCAGCCTTCCAGGCAGAGACTCAGGAGCTGCTGGATGTGATTG TTGCTGATCTTGACGGAGGGACAGTGACTGTCCCTGAGTGTGTGCACATTCCACCCCTGCCAGAGCCACT ACAAAGCCAGACCCACAATGTTCTGAGCATGGTCCTGGATCCAGAACTGGAGTTGGCTGACCTTGCCTTC CCACCCCCTACAACATCTGCTTCCTCCCTGAAAATGCAGGACAAGGAGCTCCGTGCTGTCTTTCTGCGGC TCTTTGCTCAGCTCCTGCAAGGCTACCGCTGGTGCCTGCACATCGTGCGCATCCACCCAGAGCCCGTCAT CCGCTTCCATAAGGCAGCATTCTTAGGCCAGCGTGGACTGGTGGAGGATGATTTCCTGATGAAAGTATTG GAGGGCATGGCCTTCGCGGGCTTTGTATCTGAGCGTGGGGTTCCCTACCGTGCCACAGACCTGTTTGATG AGCTGGTGGCTCATGAGGTAGCACGGATGCGAGCAGATGAGAGCCATCCCCACCGCGTCCTGCGTCATGT CCAGGAACTGGCAGAGCAACTTTATAAGAATGAAAACCCGTACCCAGCTGTGGCGATGCACAAAGTGCAG AGGCCAGGAGAGGCCAGTCACCTGCGGCGGACCCACCGGCCATTCCCCCGGCTAGATGAGGGCACAATTC AGTGGATTGTGGACCAGGCTGCGGCCAAGATGCAGGGCGCACCTCCAGCTGTCAAGGCTGAGCGGAGGAG CACTGTGCCTTCAGGGCCCCCCATGACGGCCATACTGGAGCGCTGTAGTGGGCCTCATATCAACAGTGCA CGTCGCTTGGAGGTAGTACGGAATTGCATCTCCTATGTGTTTGAAGGGAAAATGCTTGAGGCAAAGAAGT TGCTTCCGGCTGTACTCAGGGCCCTGAAGGGGCGAGCTGCCCGTCGCTGCCTTGCCCATGAGCTTCACCT GCACGTACAGCAGAACCGGGCAGTTCTGGATCATCAGCAGTTTGACTTTGTCGTCCGCATGATGAATTGC TGCCTGCAGGATTGCACTTCCCTGGATGAGCATGGCATTGCATCTGCACTGCTGCCTTTGGTCACAGCCT TCTGCAGGAAGCTGAGCCCAGGGGTGACACAGTTTGCATACAGCTGTGTTCAGGAGCACGTAGTGTGGAG CACACCTCAGTTCTGGGAGGCCATGTTCTATGGGGATGTGCAGACCCATATCCGAGCCCTCTACCTGGAG CCCTCTGATGGCGTGAGCCCCACCCAGGAGACTGGGGAGGCACAGTCTCAGGATGATGAACGATCTGCCC TGGATGTGGCTTCAGAGCAGAGGCGCCTGTGGCCAACCCTGAGCCGTGAGAAGCAGCAGGAGCTGGTACA GAAGGAGGAAAGCACTGTGTTCAGCCAGGCCATCCACTATGCCAACCGCATGAGCTACCTTCTGCTGCCT CTGGACAGCAGCAAGAGCCGGCTGCTGCGGGAGCGGGCAGGGTTGGGAGACCTGGAGAGTGCCAGCAACA GCCTGGTCACCAACAGCATGGCAGGCAGTGTGGCTGAGAGCTATGACACAGAGAGTGGCTTTGAAGATGC AGAGACGTGCGATGTGGCTGGGGCTGTAGTCCGCTTCATCAACCGCTTTGTGGACAAAGTCTGCACAGAG AGTGGGGTCACCAGCGACCACCTCAAAGGACTGCATGTCATGGTGCCAGACATTGTCCAGATGCACATTG AGACCCTGGAGGCCGTACACCGTGAGAGCAAGAGGCTGCCCCCGATACAGAAGCCCAAGCTGCTGAGGCC ACGCCTGTTGCCTGGTGAGGAGTGTGTCCTCGATGGCCTTCGAGTGTATCTGCTGCCAGACGGGCGTGAG GAGGGTGTAGGAGGCAGTGGAGGGGGCCCTGCTCTACTCCCAGCTGAGGGTGCTGTCTTCCTTACCACAT ACCGCGTCATCTTCACGGGGATGCCTACTGACCCCCTGGTGGGGGAGCAGGTGGTAGTCCGCTCCTTCCC CGTGGCTGCCTTGACCAAGGAGAAGCGCATTAGTGTGCAGACCCCTGTGGACCAGCTTCTGCAGGATGGG CTGCAGCTTCGTTCCTGCACATTCCAGCTGCTGAAAATGGCCTTTGATGAGGAGGTGGGATCTGACAGTG CTGAGCTCTTCCGAAAGCAGCTACACAAGCTTCGGTACCCACCAGACATCAGGGCCACCTTTGCATTCAC ACTTGGCTCAGCTCACACACCTGGCAGGCCGCCACGGGTTACCAAGGACAAGGGTCCTTCATTCAGAACC CTATCCCGGAACCTGATGAAGAATGCTAAAAAGACCATTGGGCGGCAGTATGTTACTCGTAAGAAGTATA ACCCCCCTGGCTGGGAGCATCGGGGCCAGCCACCCCCTGAGGACCAGGAGGACGAGATCTCAGTGTCAGA GGAGCTGGAGCCTAGCACACTGACCCCGTCCTCAGCCCTGAAGCCCTCTGACCGCATGACCATGAGCAGC CTGGTGGAACGGGCATGTTGCCGTGACTACCAGCGCCTTGGACTAGGTACCCTGAGCAGCAGCCTGAGCC GGGCCAAGTCTGAGCCCTTTCGCATCTCTCCTGTTAATCGCATGTATGCCATATGTCGCAGCTATCCAGG ATTGCTGATTGTTCCCCAGAGCATCCAGGACAACGCCCTGCAGCGTGTATCCCGCTGCTACCGCCAGAAC CGCTTCCCTGTGGTCTGCTGGCGCAGTGGGCGCTCCAAGGCTGTCTTGCTACGCTCTGGGGGCCTGCATG GCAAAGGTGTTGTTGGTCTCTTCAAGGCCCAGAATACACCTTCTCCAGGCCAGGCCCAGGCCGACTCCAG CAGCCTGGAGCAAGAGAAGTACCTGCAGGCTGTGGTCAGCTCCATGCCACGTTATGCTGACTCATCAGGA CGAAACACACTGAGTAGCTTCTCCTCAGCCCACATGGGTGGCCATGTGCCCAGCCCCCGAGCCAGGGTCA CCACGCTGTCCAACCCTTTGGCGGCCTCGGCCTCCAGATGGACTGCGTCCCGAGGGAAATGGAGCAGTGT CCGAGCCAGTGGTCGAAGCAGTGGTCTTGGTTCTGATGTGGGCTCTCGGCTAGCTGGCAGAGATCTCCTC AGTACTCCCCACACCAACGGAGCTCCACCTGATTCTGGTTTTCTACGGCCACAGCGTGCAGCCCTCTACA TCATTGGTGACAAAGCTCAGCTCAAGGGTGTGCGCCCAGATCCCTTGCAACAGTGGGAGCTGGTACCTAT TGAAGTATTTGAGGCAAGGCAGGTGAAAGCCAGCTTCAAGAAACTATTGAAAGCCTGTGTGCCTGGTTGC CCTGCCACTGAGCCCAGCCCAGCCTCTTTTCTGCGCTCACTGGAGGACTCAGAATGGCTGATCCAGATCC ACAAGCTGCTGCAGATATCGGTGCTGGTAGTGGAGCTGCTCGACTCTGGCTCCTCTGTCCTGGTGAGCCT GGAGGACGGCTGGGACATCACCACTCAGGTGGTATCCTTGGTGCAGCTGCTCTCAGACCCCTTCTACCGC ACTCTGGAAGGCTTCCGACTGCTAGTGGAAAAAGAGTGGCTGTCCTTTGGCCATCGTTTCAGCCACCGCG GGGCCCACACCCTAGCTGGGCAGAGCAGTGGCTTCACACCCGTCTTCCTGCAGTTCCTGGACTGTGTACA CCAGGTCCATTTGCAGTTCCCCATGGAGTTTGAGTTCAGTCAGTTCTACCTCAAGTTCCTTGGTTACCAC CACACATCCCGCCGTTTCCGGACCTTCTTGCTGGACTCTGATTATGAGCGTATTGAGCTGGGGCTGCTGT ATGAGGAGAAGGGTGAACGCAGGGGTCAGCTGGCCTGCAAGTCAGTGTGGGAGTATGTAGACCGGCTAAG CAAGAGGACCCCCATGTTCTACAACTACACATATGCACCCGAGGACACAGAGGTGCTGCGACCCTACAGC AATGTGTCCAATCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGGGGCCCCCTTATGACT GGGAGCTGGCCCAGGGACCCCCTGAGCCTCCAGAAGAGGAACGTCCTGATGGAGGTGCTCCCCAGAGCAG GCGCCGTGTGGTATGGCCATGCTATGACAGCCGTCCTCGAGTCCAGCCTGATGCCATCTCACGTCTGCTG GAGGAGCTGCAGCGCTTGGAGACAGAGTTAGGTCGGCCTTCTGAACGCTGGAAAGACACCTGGGACCGGG TGAAGGCTGCTCAGCGCCTTGAAAGCCGGCAAGATGGACGTGGTACCCCCAGCTCACTTCTGGTGTCGGC TGTGCCCCACCACCGCCGCTCGCTAGGCGTCTATCTACAGGAGGGGCCTGTGGGCTCTACTCTTAGCCTC AGCCTGGACAGTGACCAGAGCAGTGGCTCAACCACATCTAGCTCTCGGCAGGCCGCCCGGCGGAGCACCA GCACCCTGTACAGCCAGTTCCAGACAGCTGAGAGTGAGAACAGGTCCTATGAGGGCATCCTATACAAGAA GGGGGCCTTCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTAGACAAGACCAAGCACCAGCTGCGATAC TATGACCACCGAATGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGGAAGCTGTGGCACCTG GCACACCCACTATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTTGATGTGAAGACAACACGTCG CGTTTACAACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGGATCCAGAGCTGCCTG TCGGATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217371 representing NM_001170561
Red=Cloning site Green=Tags(s) MARLADYFVLVAFGPHPRGSGEGQGQILQRFPEKDWEDNPFPQGIELFCQPSGWQLCPERNPPTFFVAVL TDINSERHYCACLTFWEPVESTQEVVCTDNATEKEEEADGGGQARLSSTAPAQPGQLFAPKTLVLVSRLD HAEVFRNSLGLIYAIHVEGLNVSLENVIGNLLTCTVPLAGGSQRTISLGAGDRQVIQTPLVDSLPVSRCS VALLFRQLGITNVLSLFCAALTEHKVLFLSRSYQRLADACRGLLALLFPLRYSFTYVPILPAQLLEVLST PTPFIIGVNAAFQAETQELLDVIVADLDGGTVTVPECVHIPPLPEPLQSQTHNVLSMVLDPELELADLAF PPPTTSASSLKMQDKELRAVFLRLFAQLLQGYRWCLHIVRIHPEPVIRFHKAAFLGQRGLVEDDFLMKVL EGMAFAGFVSERGVPYRATDLFDELVAHEVARMRADESHPHRVLRHVQELAEQLYKNENPYPAVAMHKVQ RPGEASHLRRTHRPFPRLDEGTIQWIVDQAAAKMQGAPPAVKAERRSTVPSGPPMTAILERCSGPHINSA RRLEVVRNCISYVFEGKMLEAKKLLPAVLRALKGRAARRCLAHELHLHVQQNRAVLDHQQFDFVVRMMNC CLQDCTSLDEHGIASALLPLVTAFCRKLSPGVTQFAYSCVQEHVVWSTPQFWEAMFYGDVQTHIRALYLE PSDGVSPTQETGEAQSQDDERSALDVASEQRRLWPTLSREKQQELVQKEESTVFSQAIHYANRMSYLLLP LDSSKSRLLRERAGLGDLESASNSLVTNSMAGSVAESYDTESGFEDAETCDVAGAVVRFINRFVDKVCTE SGVTSDHLKGLHVMVPDIVQMHIETLEAVHRESKRLPPIQKPKLLRPRLLPGEECVLDGLRVYLLPDGRE EGVGGSGGGPALLPAEGAVFLTTYRVIFTGMPTDPLVGEQVVVRSFPVAALTKEKRISVQTPVDQLLQDG LQLRSCTFQLLKMAFDEEVGSDSAELFRKQLHKLRYPPDIRATFAFTLGSAHTPGRPPRVTKDKGPSFRT LSRNLMKNAKKTIGRQYVTRKKYNPPGWEHRGQPPPEDQEDEISVSEELEPSTLTPSSALKPSDRMTMSS LVERACCRDYQRLGLGTLSSSLSRAKSEPFRISPVNRMYAICRSYPGLLIVPQSIQDNALQRVSRCYRQN RFPVVCWRSGRSKAVLLRSGGLHGKGVVGLFKAQNTPSPGQAQADSSSLEQEKYLQAVVSSMPRYADSSG RNTLSSFSSAHMGGHVPSPRARVTTLSNPLAASASRWTASRGKWSSVRASGRSSGLGSDVGSRLAGRDLL STPHTNGAPPDSGFLRPQRAALYIIGDKAQLKGVRPDPLQQWELVPIEVFEARQVKASFKKLLKACVPGC PATEPSPASFLRSLEDSEWLIQIHKLLQISVLVVELLDSGSSVLVSLEDGWDITTQVVSLVQLLSDPFYR TLEGFRLLVEKEWLSFGHRFSHRGAHTLAGQSSGFTPVFLQFLDCVHQVHLQFPMEFEFSQFYLKFLGYH HTSRRFRTFLLDSDYERIELGLLYEEKGERRGQLACKSVWEYVDRLSKRTPMFYNYTYAPEDTEVLRPYS NVSNLKVWDFYTEETLAEGPPYDWELAQGPPEPPEEERPDGGAPQSRRRVVWPCYDSRPRVQPDAISRLL EELQRLETELGRPSERWKDTWDRVKAAQRLESRQDGRGTPSSLLVSAVPHHRRSLGVYLQEGPVGSTLSL SLDSDQSSGSTTSSSRQAARRSTSTLYSQFQTAESENRSYEGILYKKGAFMKPWKARWFVLDKTKHQLRY YDHRMDTECKGVIDLAEVEAVAPGTPTIGAPKTVDEKAFFDVKTTRRVYNFCAQDVPSAQQWVDRIQSCL SDA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001170561 |
| ORF Size | 5679 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170561.1, NP_001164032.1 |
| RefSeq Size | 6268 bp |
| RefSeq ORF | 5682 bp |
| Locus ID | 77980 |
| UniProt ID | Q6ZPE2 |
| Gene Summary | Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts.[UniProtKB/Swiss-Prot Function] |
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