Tnrc6a (NM_144925) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2010321I05Rik; 3110054G10Rik; AW557223; CAGH26; D130023A07Rik; GW182; Tnrc6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225023 representing NM_144925 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAATTGGAAGCTAAAGCTACCAAAGACGTAGAGAGAAATCTTAGCAGGGATTTAGTGCAAGAAG AAGAACAGTTGATGGAAGAAAAGAAAAAGAAAAAAGACGACAAGAAAAAGAAGGAAGCTGCTCAAAAGAA GGCCACTGAACAAAAAATCAAAGTGCCAGAACAGATAAAGCCCAGTGTAAGCCAGCCTCAGCCTGCCAAC TCTGATAACGGCACTTCCACAGCAACCAGCACTAATAATAATGCCAAGCGAGCTACAGCCAGCAATCAGC AGCCGCCGCCACCGCAGCAGCAGCAGCCGCAGCAAGAGCAGCAGCAGCAGCAGCCACAAGCCTTGCCTCG GTATCCACGTGAAGTACCTCCACGATTTCGCCACCAGGAACACAAACAGCTTCTGAAGAGGGGTCAGCAT TTTCCTGTCATAGCAGCAAACTTGGGATCTGCTGTTAAGGTGTTAAACAGCCAATCAGAAAGCAGTGCTG TAACAAATCAACAGCCACAAAATAACGGAGAGGTGCAGAACAGCAAGAGCCAGTCAGATATAAATCATAA TACTTCAGGATCCCATTATGAAAATTGCCAGCGGGGACCTGTGTCTTCTACAAGTGACTGTAGCACAAGC TGTAAGAATGCTGTAAACGACTTGTTGGAAAAAGAAGCATGGCCCTCAGCCCCTGGCAGTGATCCTGAGT TGGCTCCAGAATGTATAGATGCTGATTCTGCCTCCAATTCTGAGTCAGAGAGAAACATCACTGTCATGGC TTCAGGGAACACAGGTGGTGAGAAAGATGGCCTTCGGAACAGCACTGGACTTGGTTCTCAAAGCAAATTT GTGGTTGGTAGCAGCAGCAATAATGTGGGCCATGGAAGTAGTACTGGCCCATGGGGCTTTCCCCATGGAG CCCTAATAAGCACATGTCAGGTCTCTGTGGATGCTCCTGAAAGCAAACCAGAAAGTAGTAACAATAGGAT GAATGCTTGGGGCACTGTAAGTTCTTCATCAAATGGAGGGTTAAATCCAAGCACTTTGAATTCAGCTAGC AACCATGGTGCCTGGCCAGTATTAGAAAACAATGGACTTGCCCTAAAAGGGCCTGTAGGGAGTGGGAGTT CTGGCATCAATATTCAGTGTAGTACCATAGGCCAGATGCCTAACAATCAGAATATCAACTCTAAAGTCAG TGGCTCTTCTACCCATGGTACCTGGGGTAGCCTTCAGGAAACTTGTGAGCCTGAAGTAAGTGGTACACAG AAGGTTTCATTCAGTGGTCAACCTCAGAATATCACCACTGAAACGACTGGACCAAATAACACTACTAACT TTATGACCTCTAGTTTACCAAACTCCGGTTCAGTACAAAATAATGAACTGCCTACTAGTAATCCAGGGGC CTGGCGTGTGAGCACAATGAATCATCCTCAGATACAGGCTCCGTCAGTTATGAATGGCACTTCCCTTTCT CACCTTAGTAATGGAGAGTCAAAAACTGGAGGCTCCTACGGTACTACATGGGGTGCCTATGGTTCTAATT ACTCTGGCGACAAATGTGCAGGCCCTAATGGCCAAGCCAATGGTGACACTGTGAATGCAACTCTAATGCA GCCTGGCATAAATGGGCCTATGGGCACTAACTTTCAAGTTAATACAAATAAAGGGGGAGGTGTATGGGAG CCTGGGACAGTGAATTCCCAGAGTTCACCATGGGGAAGTGGAAATGGTGCAAATTCTGGAGGAAGTCGAA GAGGATGGGGAAGTCCTGCACAGAACACTGGCACTGGTCTATCCAGTGTCGAGTGGAACAAACTGCCTAG CAACCAGCATTCCAATGACAGTGCAAATGGCAATGGTAAGAAGCTTACAAATGGATGGAAATCTACTGAG GAAGACGATCAGGGTTCTGCCACATCTCAGACAAATGAGCAAAACAGTGTGTGGGCCAAAGCAGGAGGCA CAGTGGAGAGTGATGGTAGTGCAGAGAGCACTGGACGCCTTGAAGAAAAAGTAACCGGGGAAAGTCAGAG TAGAGATAGAAGAAAAATTGATCAGCACACATTACTCCAAAGCATTGTAAACAGAACTGACTTAGATCCA CGTGTCCTATCCAACTCTGGGTGGGGACAGACTCCTATTAAGCAGAATACTGCCTGGGATACAGAGACAT CACCAAGAGGGGAAAGAAAGACTGACAATGGGACAGAGGCCTGGGGAAGCTCTGCAACACAGACTTTTAA CTCAGGGGCATGTACAGATAAGACTAGCCCTAATAGTAATGATACCTCATCTGTATCAGGGTGGGGTGAT CCCAAACCTACTCTGAGGTGGGGAGATTCCAAAGGCTCAAACTGCCAGGGGGGGTGGGAAGATGACTCTG CTGCTACAGGAATGATCAAGAGCAACCAGTGGGGGGGTTGCAAGGAAGACAAGTCTACATGGAATGATTC GCAAAAGAGCAAACAAGGCTGGGGTGACGGACAAAAGTCAAGCCAAGGTTGGTCCATTTCTGCCGGTGAT AACTGGGGAGAATCTTCCAGGAGTAACCATTGGGGTGAGGCCAATAAGAAATCCAGCTCAGGAGGCAGTG ACAGTGACAGGTCCATTTCTGGTTGGAACGAACTTGGGAAAACTAGTTCTTTTACTTGGGGAAATAATAT AAATCCAAATAACTCATCGGGATGGGATGAATCTTCTAAACCGAACTCTTCCCAGGGGTGGGGAGACCCT CCAAAGTGTAATCAGTCTCTAGGTTGGGGAGATTCATCAAAGCCAGTTAGTTCTCCAGATTGGAACAAGC AACAAGACATTGTTGGATCATGGGGAATCCCACCAGCCACCAGCAAGCCTCCTGGTACAGGCTGGCTCGG GGGACCTATTCCTGCTCCAGCAAAGGAGGAAGAACCCACAGGCTGGGAGGAGCCATCCCCAGAATCTATA CGAAGAAAAATGGAGATCGATGATGGAACTTCAGCTTGGGGAGATCCAAGCAAATACAACTACAAAAATG TGAACATGTGGAATAAAAACATCCCGGAAGCCAGCGGCCGCTCAGACCAGCAAGCGCAGATGCACCGGCT ATTGCCAGCCGCAAGTGCCGTCTCAAGCAAGGAGACCAGCAGTGGCTCTGGCTGGGGTGAGCCTTGGGCA GAGCCTTCTACTCCAGCCACAACTGTGGATAATGGTACTTCAGCCTGGGGCAAGCCCATAGACAGTGGTC CCAGCTGGGGAGAACCCATTACTGCAGCATCCAATGCATCCACGTGGGGCTCCAGCTCTGTTGGTCCACA GTCATTAAGCAAATCTGGGCCCAAATCTATGCAAGATGGCTGGTGTGGTGATGATATGCCATTGCCTGGA AGTCGACCCACTGGCTGGGAAGAGGAGGAGGATGTAGAGATTGGAATGTGGAACAGTAACTCATCTCAAG AGCTTAACTCATCTTTAAATTGGCCACCATATACCAAGAAAATGTCATCAAAGGGTCTGAGTGGCAAAAA AAGGAGAAGGGAAAGGGGAATGATGAAAGGCGGAAACAAACAAGAGGACGCATGGATAAATCCATTCGTT AAACAGTTTTCAAATATCAGTTTTTCGAGAGACTCACCAGAAGAAAATGTACAGAGCAATAAGATGGATC TTTCTGGAGGAATGTTACAAGACAAGCGGATGGAGATTGATAAACATAGCCTGAATATTGGTGATTACAA TCGAACGGTCGGGAAAGGCCCTGGGTCTCGGCCTCAGATTTCCAAAGAGTCTTCCATGGAGCGCAATCCT TACTTTGATAAGAATGGCAATCCCAATATGTTTGGTGTTGGAAACACAGCAGCACAACCCCGGGGCATGC AGCAGCCTCCAGCACAACCTCTCAGTTCATCTCAGCCTAATCTTCGTGCTCAAGTGCCTCCTCCATTACT CTCCCCTCAGGTTCCAGTTTCATTGCTGAAGTATGCACCAAACAACGGTGGCCTGAATCCGCTCTTTGGC CCTCAACAGGTAGCCATGCTGAACCAGCTATCCCAACTAAACCAGCTTTCTCAGATCTCCCAGTTACAGC GATTGTTAGCGCAGCAGCAGAGGGCACAGAGTCAGAGAAGCGCGCCTTCTGCTAACCGACAGCAGCAGGA CCAGCAGGGTCGACCTCTTAGTGTGCAGCAGCAGATGATGCAACAGTCTCGTCAACTTGATCCAAGCCTG CTGGTGAAGCAGCAGACTCCGCCTTCTCAGCAGCCGCTCCATCAGCCAGCCATGAAGTCCTTCCTTGACA ATGTCATGCCCCACACTACACCCGAGCTGCAGAAAGGGCCATCGCCAGTAAATGCTTTCAGCAACTTTCC TATAGGCTTGAACTCAAACTTGAATGTAAATATGGATATGAACAGTATTAAAGAGCCACAGTCACGACTA AGGAAGTGGACAACAGTGGACAGCATGTCTGTGAACACGTCGTTGGATCAGAACTCCAGCAAACATGGTG CTATTTCAAGTGGTTTTAGGCTGGAAGAGTCTCCATTTGTTCCCTATGACTTTATGAATAGCAGTACTTC ACCAGCCAGTCCTCCAGGTTCAATAGGAGATGGCTGGCCACGTGCCAAATCGCCTAACGGCTCTAGCAGT GTTAACTGGCCACCAGAATTTCGCCCTGGTGAACCATGGAAAGGTTATCCAAACATTGACCCTGAAACTG ACCCTTACGTCACTCCTGGCAGTGTCATAAACAGTCTTTCAATTAATACTGTGCGGGAAGTTGACCACCT CAGGGACAGGAACAGTGGGTCATCCTCATCCTTGAACACCACGCTGCCTTCAACTAGTGCCTGGTCATCC ATTCGTGCCTCCAACTACAATGTTCCCCTCAGCAGTACAGCACAAAGCACTTCAGCCAGAAATAGTGATT CCAAATTGACGTGGTCTCCTGGTTCAGTTACAAACACCTCTCTGGCTCATGAGCTGTGGAAAGTCCCTTT GCCACCTAAAAACATCACTGCTCCGTCCCGCCCACCTCCGGGGCTGACTGGTCAGAAGCCACCCTTGTCT ACGTGGGATAATTCTCCCCTTCGTGTAGGTGGAGGATGGGGAAATTCTGATGCCAGATATACCCCAGGCT CCAGCTGGGGTGAGAGCAGCTCAGGGAGAATAACCAATTGGCTTGTTCTGAAGAACCTCACACCTCAGAT TGATGGCTCAACTCTGCGCACCCTGTGCATGCAGCATGGCCCACTGATTACATTCCATCTGAACCTCCCA CATGGAAATGCTCTGGTCCGTTACAGTTCAAAAGAAGAGGTAGTGAAGGCACAAAAGTCTCTGCACATGT GTGTGCTGGGGAACACTACTATTCTCGCTGAGTTTGCCAGTGAAGAGGAGATCAGTCGCTTCTTTGCACA AAGTCAGTCTCTGACCCCTTCTCCTGGCTGGCAGTCTCTCGGGTCCAGCCAGAGCCGGCTGGGCTCTCTC GACTGTTCCCACTCGTTCTCCAGCCGGACTGATGTCAATCACTGGAATGGTGCTGGGCTGTCGGGAGCTA ACTGTGGAGACCTTCACGGCACTTCCCTCTGGGGGACCCCACATTATTCCACAAGCCTGTGGGGTCCCCC GAGCAGCGACCCCCGGGGAATTAGCAGCCCATCCCCCATTAATGCTTTTCTTTCTGTTGACCACCTGGGT GGGGGTGGGGAGTCCATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_144925
Insert Size	5691 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_144925.3, NP_659174.3
RefSeq Size	8493 bp
RefSeq ORF	5691 bp
Locus ID	233833
UniProt ID	Q3UHK8
Gene Summary	This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein is highly similar to a human protein that functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The human protein associates with messenger RNAs and argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies, and inhibiting its expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.