Myh9 (NM_022410) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fltn; Myhn-1; Myhn1; NMHCIIA; NMMHC-A; NMMHC-IIA; TU72.6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225079 representing NM_022410 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGCAGGCTGCAGACAAGTACCTCTATGTGGATAAAAACTTCATCAATAACCCGCTGGCCCAAG CTGACTGGGCTGCCAAGAAGTTGGTATGGGTGCCTTCCAGCAAGAATGGCTTTGAACCAGCTAGCCTCAA GGAGGAGGTGGGAGAAGAGGCCATTGTAGAGCTGGTAGAGAATGGGAAGAAGGTGAAGGTGAACAAGGAC GACATCCAGAAGATGAACCCACCCAAGTTCTCCAAGGTGGAGGACATGGCAGAGCTCACGTGCCTCAACG AAGCTTCGGTGCTGCACAACCTCAAGGAGCGATACTACTCAGGGCTTATCTACACCTATTCAGGCCTGTT CTGTGTGGTCATCAACCCTTATAAGAACCTGCCCATCTACTCAGAGGAGATCGTGGAGATGTACAAGGGC AAGAAGAGGCACGAGATGCCACCCCACATCTACGCCATCACAGATACTGCCTACCGGAGCATGATGCAGG ACCGGGAAGATCAGTCCATCCTGTGCACGGGGGAGTCTGGAGCAGGGAAGACAGAGAACACCAAGAAAGT CATCCAGTACCTGGCACATGTGGCCTCCTCACACAAGAGCAAGAAGGACCAGGGGGAGTTGGAGCGGCAG CTGCTACAGGCCAACCCTATCCTAGAGGCCTTTGGAAACGCCAAGACGGTGAAGAATGACAACTCCTCTC GATTCGGTAAATTCATTCGTATCAACTTTGATGTCAATGGCTACATTGTTGGTGCCAACATTGAGACTTA TCTTCTGGAGAAATCTCGTGCTATCCGCCAAGCCAAAGAGGAGCGGACCTTCCACATCTTCTACTACCTG CTGTCTGGGGCCGGAGAACACCTGAAGACTGATCTCCTGTTGGAGCCATACAACAAATACCGCTTCCTGT CCAACGGGCACGTCACCATCCCTGGGCAGCAGGACAAGGACATGTTCCAGGAGACAATGGAGGCCATGAG AATTATGGGCATCCCAGAGGATGAGCAGATGGGCTTGCTGCGGGTCATCTCTGGGGTCCTTCAGCTTGGC AACATTGCCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCGGACAACACAGCTGCTCAAAAGG TGTCCCACCTCCTGGGGATCAATGTGACCGACTTCACCAGAGGCATCCTCACCCCACGCATCAAGGTGGG CAGAGACTATGTGCAGAAGGCGCAGACTAAAGAGCAGGCTGACTTTGCCATTGAGGCCTTGGCCAAGGCT ACCTATGAGCGGATGTTCCGCTGGCTGGTGCTTCGCATCAACAAAGCTCTGGACAAGACCAAGAGGCAGG GCGCCTCATTTATCGGGATCCTGGACATCGCTGGCTTTGAGATCTTTGATCTGAACTCCTTCGAGCAGCT GTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACCATGTTCATCCTGGAGCAGGAG GAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTCGGCCTGGACCTGCAGCCCTGCATCGACC TCATTGAGAAGCCGGCGGGTCCCCCAGGCATCCTGGCCCTGCTAGATGAGGAGTGCTGGTTTCCTAAGGC CACTGACAAGAGCTTCGTGGAGAAGGTGGTGCAGGAGCAGGGCACCCACCCCAAGTTCCAGAAGCCCAAG CAACTGAAGGACAAGGCTGATTTCTGCATTATCCACTATGCCGGCAAGGTGGACTATAAAGCTGACGAGT GGCTGATGAAGAACATGGACCCCTTGAACGACAACATCGCCACGCTGCTTCACCAGTCCTCAGACAAGTT TGTCTCTGAGCTGTGGAAGGATGTGGATCGGATCATTGGCTTGGACCAAGTGGCTGGAATGTCCGAGACA GCACTACCTGGTGCCTTCAAGACCCGGAAGGGCATGTTCCGTACTGTCGGACAGCTGTACAAGGAGCAGC TGGCCAAGCTCATGGCCACGTTGAGGAATACCAACCCCAACTTCGTGCGCTGCATCATTCCCAACCATGA GAAGAAGGCCGGCAAACTGGACCCGCACTTGGTGCTGGACCAGCTGCGCTGCAATGGCGTCCTTGAGGGC ATCCGGATCTGCCGCCAGGGCTTTCCCAACAGGGTGGTCTTCCAGGAGTTCCGGCAGAGGTATGAGATCC TCACCCCCAACTCCATCCCGAAGGGCTTCATGGATGGCAAGCAAGCGTGTGTGCTCATGATCAAAGCCTT GGAGCTTGACAGCAACCTGTACCGCATCGGCCAGAGCAAAGTGTTCTTCCGGGCAGGAGTGCTAGCCCAC CTGGAGGAAGAGCGGGACCTGAAGATCACCGATGTCATCATTGGCTTCCAGGCCTGCTGCAGGGGCTACC TGGCCAGGAAGGCCTTTGCCAAGAGGCAGCAACAGCTGACCGCCATGAAGGTCCTACAGAGGAACTGTGC TGCGTACCTCAGGCTGCGCAACTGGCAGTGGTGGAGGCTCTTCACCAAGGTCAAGCCCCTGTTGAACTCA ATAAGACATGAGGATGAGCTGTTAGCCAAGGAGGCGGAACTGACAAAGGTTCGAGAGAAACATCTGGCTG CAGAGAACAGGCTGACAGAGATGGAGACGATGCAGTCTCAGCTCATGGCAGAGAAGCTGCAGCTTCAGGA GCAGCTGCAGGCGGAGACAGAGCTGTGTGCCGAGGCTGAGGAGCTCCGGGCCCGTCTGACAGCGAAGAAG CAGGAGCTGGAGGAGATCTGCCATGACCTGGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTGCCAGTACC TGCAGGCCGAGAAGAAGAAGATGCAGCAGAACATCCAGGAACTTGAGGAGCAGTTGGAGGAGGAGGAGAG CGCCCGGCAGAAGCTGCAGCTTGAGAAGGTGACCACCGAGGCCAAGCTGAAGAAACTGGAGGAGGACCAG ATCATCATGGAGGACCAGAACTGCAAACTGGCCAAGGAGAAGAAACTGCTGGAAGACAGAGTAGCTGAAT TCACTACCAACCTCATGGAAGAGGAGGAGAAGTCCAAGAGCCTGGCCAAGCTCAAGAACAAGCACGAGGC AATGATCACCGACCTGGAAGAGCGCCTCCGTAGGGAGGAGAAGCAGAGGCAGGAGTTGGAGAAGACCCGT CGCAAGCTGGAGGGAGACTCCACAGACCTCAGTGACCAGATTGCTGAGCTCCAGGCGCAGATAGCAGAGC TCAAGATGCAGCTGGCCAAGAAGGAGGAGGAGTTGCAGGCTGCCTTGGCCAGAGTGGAAGAAGAAGCTGC TCAGAAGAATATGGCCCTGAAGAAGATCCGAGAACTGGAAACTCAGATCTCTGAGCTCCAGGAGGACCTG GAGTCGGAGCGAGCCTCCAGGAATAAAGCCGAGAAGCAGAAACGGGATCTGGGAGAGGAGCTGGAGGCGC TGAAGACAGAGCTGGAGGACACGCTGGACTCCACGGCTGCCCAGCAGGAGCTGAGGTCGAAGCGTGAGCA GGAGGTGAGCATCCTGAAGAAGACTCTGGAGGACGAGGCCAAGACCCATGAGGCCCAGATCCAGGAGATG AGGCAGAAGCACTCACAGGCTGTGGAGGAGCTGGCAGATCAGTTGGAGCAGACGAAGCGGGTAAAAGCTA CCCTTGAGAAGGCGAAGCAGACCCTGGAGAATGAGCGGGGAGAGCTGGCCAATGAGGTGAAGGCCCTGCT GCAAGGCAAGGGCGACTCAGAGCACAAGCGCAAGAAGGTGGAGGCGCAGCTGCAAGAACTGCAGGTCAAG TTCAGCGAGGGAGAGCGCGTGCGAACCGAACTGGCCGACAAGGTCACCAAGCTGCAGGTTGAACTGGACA GTGTGACCGGTCTCCTTAGCCAGTCTGACAGCAAGTCCAGCAAGCTTACGAAGGACTTCTCTGCGCTGGA GTCCCAGCTTCAGGACACACAGGAGTTGCTCCAGGAGGAGAACCGGCAGAAGCTGAGCCTGAGCACCAAG CTCAAGCAGATGGAGGATGAGAAAAACTCCTTCAGGGAGCAGCTGGAGGAGGAGGAGGAGGCCAAGCGCA ACTTGGAGAAGCAGATCGCCACGCTCCATGCCCAGGTGACCGACATGAAGAAGAAGATGGAGGACGGTGT AGGGTGCCTGGAGACTGCAGAGGAGGCGAAGCGGAGGCTTCAGAAGGACTTGGAAGGCCTGAGCCAGCGG CTTGAGGAGAAGGTGGCTGCCTACGATAAGCTGGAGAAGACCAAGACACGGCTGCAGCAGGAGCTGGACG ACCTGCTGGTTGACCTGGACCACCAGCGGCAGAGCGTCTCCAACCTGGAAAAGAAGCAGAAGAAGTTCGA CCAGCTCCTAGCCGAGGAGAAGACCATCTCGGCCAAGTATGCAGAGGAGCGTGACCGAGCTGAGGCTGAG GCCCGTGAGAAGGAGACAAAGGCGCTATCACTGGCCCGGGCGCTTGAGGAGGCCATGGAGCAGAAGGCAG AGCTGGAGCGGCTCAACAAGCAGTTCCGCACGGAGATGGAGGACCTCATGAGCTCCAAGGATGACGTGGG CAAGAGTGTCCACGAGCTGGAGAAGTCCAAGCGGGCCTTGGAGCAGCAGGTGGAGGAGATGAAGACCCAG CTGGAGGAGCTGGAGGATGAGCTGCAGGCCACGGAGGATGCCAAGCTCCGCCTGGAGGTGAACCTGCAGG CCATGAAGGCCCAGTTTGAGCGGGATCTGCAGGGCCGGGATGAACAGAGCGAGGAGAAGAAGAAGCAGCT GGTCAGACAGGTGCGGGAGATGGAGGCGGAGCTGGAGGATGAGAGGAAGCAGCGCTCCATGGCCATGGCC GCACGCAAGAAACTGGAGATGGATCTGAAGGACCTGGAGGCACACATTGACACAGCCAATAAGAACCGGG AAGAGGCCATCAAACAGCTGCGGAAGCTTCAGGCCCAGATGAAGGACTGCATGCGGGAGCTGGACGACAC GCGCGCCTCCCGGGAGGAGATCCTGGCGCAGGCCAAGGAGAATGAGAAGAAGCTGAAGAGCATGGAGGCC GAGATGATTCAGCTGCAGGAGGAACTGGCAGCTGCTGAGCGTGCTAAGCGTCAGGCCCAACAGGAACGGG ACGAGCTGGCTGATGAGATCGCCAACAGCAGTGGCAAAGGGGCCCTAGCATTAGAGGAGAAGCGGCGACT GGAGGCCCGCATTGCCCAGCTGGAGGAGGAGCTGGAGGAGGAACAGGGCAACACGGAGCTGATCAACGAT CGGCTGAAGAAGGCCAACCTGCAGATCGACCAAATAAACACCGACCTGAACCTGGAACGCAGCCACGCAC AGAAGAATGAGAATGCGCGACAGCAGCTGGAACGCCAGAACAAGGAGCTCAAGGCCAAGCTGCAGGAAAT GGAGAGTGCTGTCAAGTCCAAATACAAGGCCTCCATCGCGGCCTTGGAGGCCAAAATTGCACAGCTGGAG GAACAGCTGGACAACGAGACCAAGGAGCGCCAGGCAGCCTCCAAGCAGGTGCGCCGGACGGAGAAGAAGC TGAAGGACGTGCTGCTGCAGGTGGAGGACGAGCGGAGGAACGCGGAACAGTTCAAGGACCAGGCTGACAA GGCGTCCACCCGCCTGAAGCAGCTTAAACGGCAGCTAGAGGAGGCTGAAGAGGAGGCCCAGCGGGCCAAT GCCTCACGCCGGAAGCTGCAGCGTGAGCTGGAAGATGCCACAGAGACCGCTGATGCTATGAACCGCGAGG TCAGCTCCCTGAAGAACAAACTGAGGCGTGGGGACCTGCCATTTGTCGTGACTCGCCGAATTGTTCGGAA AGGCACTGGCGACTGCTCAGACGAGGAGGTCGACGGTAAAGCAGATGGGGCCGATGCCAAGGCAGCTGAA TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022410
Insert Size	5883 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022410.3, NP_071855.2
RefSeq Size	7439 bp
RefSeq ORF	5883 bp
Locus ID	17886
UniProt ID	Q8VDD5
Gene Summary	During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function]

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