Tnc (NM_011607) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI528729; C130033P17Rik; cytotactin; Hxb; Ten; tenascin-C; TN; TN-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225127 representing NM_011607 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGTGACCTGGCTACTGCCAGGCATCTTTCTAGCTTTGTTTGCCCTCACTCCCGAAGGTGGGG TCCTCAAGAAAATCATCAGGCACAAGCGAGAGAGTGGGCTAAACATGACCCTGCCAGAAGAGAATCAGCC AGTGGTGTTCAACCATATCTACAACATCAAGTTGCCCATGGGTTCCCAATGCTCAGTGGATCTGGAGTCA GCGAGTGGGGAGAAAGACCTGACCCCCACGCCAGAGTCCAGTGGGAGCTTCCAGGAACATACAGTGGATG GGGAAAACCAAATTGTATTCACACACCGCATCAACATCCCCCGCCGGGCCTGTGGCTGTGCTGCAGCTCC AGATGTGAAGGAGCTCCTGAGCAGGCTGGAGGAGCTGGAGTTGCTGGTATCGTCTCTAAGGGAGCAGTGC ACCATGGGTACAGGCTGTTGCCTCCAACCTGCAGAAGGTCGTCTGGACACCAGGCCCTTCTGCAGCGGAC GGGGCAACTTCAGTGCAGAAGGCTGCGGCTGTGTCTGTGAACCAGGCTGGAAAGGCCCCAACTGCTCTGA GCCTGACTGCCCTGGGAACTGTAATCTCAGAGGCCAGTGCCTTGACGGCCAGTGTATCTGTGACGAGGGT TTCACTGGGGAAGACTGCAGCCAGCTGGCCTGTCCGAACGACTGCAATGACCAGGGCAGGTGTGTGAACG GGGTCTGTGTGTGCTTCGAAGGCTATGCCGGCCCTGACTGTGGCCTGGAAGTCTGCCCAGTGCCCTGCAG CGAGGAACACGGGATGTGTGTGGATGGCAGGTGTGTGTGCAAAGATGGCTTTGCGGGTGAGGACTGCAAC GAGCCCCTTTGCCTCAACAACTGCTACAATCGTGGGCGGTGTGTGGAGAACGAGTGTGTCTGCGACGAGG GCTTCACTGGCGAGGACTGCAGTGAGCTCATTTGCCCCAACGACTGCTTCGACCGCGGTCGCTGCATCAA TGGCACCTGCTACTGTGAAGAAGGTTTCACGGGCGAAGACTGCGGTGAGCTCACCTGCCCCAACGACTGT CAGGGCCGTGGCCAGTGTGAGGAGGGACAGTGTGTTTGCAACGAGGGCTTTGCAGGAGCAGACTGCAGTG AAAAGCGGTGTCCCGCCGATTGTCACCACCGTGGCCGCTGCCTCAACGGGCAGTGTGAGTGTGACGATGG GTTCACAGGGGCTGACTGTGGGGATCTCCAGTGTCCCAATGGCTGCAGTGGGCATGGCCGTTGTGTCAAC GGGCAGTGCGTGTGTGACGAGGGCTATACCGGAGAAGACTGTAGCCAGCGGCGATGCCCCAATGACTGTC ACAACCGGGGTCTCTGCGTACAGGGCAAGTGCATATGTGAACAAGGCTTCAAGGGCTTTGACTGTAGTGA GATGAGCTGTCCCAATGACTGCCACCAACATGGCCGCTGTGTGAATGGCATGTGTATCTGTGATGACGAC TACACTGGGGAAGACTGCAGAGACCGGCGCTGTCCCCGGGACTGTAGCCAGCGGGGGCGCTGTGTAGACG GACAGTGCATCTGTGAGGATGGCTTCACTGGCCCTGACTGTGCTGAGCTTTCCTGCCCCAGTGACTGCCA CGGCCATGGCCGCTGTGTGAATGGCCAATGCATCTGCCACGAGGGCTTCACTGGCAAAGACTGCAAAGAG CAAAGGTGCCCCAGTGATTGCCATGGCCAAGGCCGCTGTGAGGACGGCCAGTGTATCTGCCATGAGGGCT TTACGGGCCTGGACTGTGGGCAGCGCTCCTGTCCCAATGACTGCAGCAACCAAGGACAATGTGTGTCAGG CCGCTGCATCTGCAATGAAGGTTACACAGGGATAGACTGCTCTGAGGTGTCCCCTCCCAAAGACCTTATT GTGACAGAAGTAACAGAGGAGACTGTAAATCTGGCATGGGACAATGAGATGCGGGTCACTGAGTACCTCA TTATGTACACACCCACCCATGCTGATGGCCTAGAGATGCAGTTCCGTGTGCCTGGGGACCAGACATCCAC CACCATTCGGGAGCTGGAACCAGGGGTGGAGTACTTCATTCGTGTGTTCGCCATCTTGGAAAACAAGAGG AGCATCCCTGTCAGTGCCAGGGTTGCCACCTATTTGCCTGCACCTGAAGGTCTAAAATTCAAGTCTATCA AGGAGACATCTGTGGAAGTAGAGTGGGATCCTCTAGACATTGCTTTCGAAACATGGGAGATCATTTTCCG AAATATGAACAAAGAAGATGAGGGAGAGATCACAAAAAGTCTGAGGAGACCAGAGACCTCCTACCGCCAA ACTGGCCTTGCTCCTGGCCAAGAGTATGAAATATCTCTGCACATTGTGAAAAACAACACCCGAGGCCCCG GCTTGAAGAAAGTAACCACAACCCGCCTGGATGCCCCCAGCCATATTGAGGTGAAAGATGTCACAGACAC CACAGCACTGATCACCTGGTTCAAGCCCTTGGCTGAAATTGATAGCATTGAGCTCTCCTATGGCATCAAG GATGTACCTGGAGACCGTACCACCATCGACCTCACACACGAAGACAACCAGTACTCCATCGGGAACCTGA GACCTGACACGGAGTATGAGGTGTCCCTCATCTCCCGCAGAGTGGACATGGCAAGCAACCCTGCCAAAGA GACCTTCATCACAGGCCTGGATGCTCCCAGGAATCTCCGCCGTGTCTCACAGACAGACAACAGCATCACC TTGGAGTGGAGGAACGTCAAGGCAGACATCGACAGTTACAGAATTAAGTATGCCCCTATCTCTGGAGGTG ACCATGCTGAGATAGATGTTCCAAAGAGCCAGCAAGCCACAACCAAAACCACACTCACAGGTCTAAGGCC CGGAACTGAATATGGGATTGGTGTTTCTGCTGTCAAGGGAGACAAGGAGAGTGATCCAGCAACCATCAAT GCGGCCACAGAAATTGATGCACCCAAGGACTTACGGGTGTCTGAAACCACACAAGACAGTCTGACGTTTT TCTGGACGACACCCCTGGCCAAGTTTGATCGTTACCGCCTCAACTACAGCCTCCCCACAGGCCAGTCGAT GGAAGTCCAGCTGCCAAAGGATGCCACCTCCCATGTCCTGACAGACCTGGAGCCAGGGCAAGAATACACT GTTCTCCTCATTGCTGAGAAGGGCAGACACAAGAGCAAGCCTGCACGTGTGAAGGCATCCACGGAAGAAG TGCCTTCCCTGGAAAATCTCACTGTGACTGAGGCCGGCTGGGATGGCCTCAGACTCAACTGGACTGCAGA TGACCTGGCCTATGAGTACTTTGTCATTCAGGTACAGGAAGCCAACAATGTGGAGACTGCTCACAACTTC ACAGTACCTGGTAACCTCCGGGCTGCAGACATCCCAGGCCTCAAGGTTGCCACTTCTTATAGAGTCTCCA TCTATGGGGTAGCCCGGGGCTATAGAACACCAGTGCTCTCTGCCGAGACCTCCACAGGGACAACTCCCAA TCTGGGAGAGGTCACTGTGGCCGAGGTGGGCTGGGATGCCCTCACGCTCAACTGGACTGCTCCAGAAGGA GCCTATAAGAACTTTTTCATTCAGGTGCTAGAGGCTGACACGACCCAGACTGTCCAGAACCTCACAGTCC CAGGAGGACTGAGGTCAGTGGACCTGCCTGGGCTCAAAGCAGCCACCCGCTACTACATCACCCTTCGAGG GGTCACCCAGGACTTCGGCACGGCCCCTCTCTCTGTTGAGGTCTTGACAGAGGATCTCCCTCAGCTGGGA GGCTTGTCTGTGACTGAGGTCAGCTGGGACGGCCTCACACTCAACTGGACCACAGATGACCTGGCCTATA AGCACTTTGTCGTTCAGGTGCAGGAGGCCAACAATGTGGAGGCCGCTCAGAACCTCACGGTACCCGGCAG CCTCAGAGCTGTGGACATCCCAGGCCTCAAGGCCGACACCCCTTATAGAGTCTCCATCTACGGGGTCATC CAGGGCTATAGAACACCGATGCTCTCTACTGACGTCTCCACAGCCAGAGAACCTGAAATTGGAAACTTAA ATGTTTCTGATGTAACTCCTAAGAGCTTCAATCTCTCCTGGACAGCTACCGACGGGATCTTCGACATGTT TACTATTGAAATTATTGATTCTAATAGGTTGCTGCAAACAGCAGAACATAATATATCTGGTGCTGAACGG ACTGCCCACATCTCAGGGCTTCCACCTAGTACTGATTTCATTGTCTACCTCTCTGGAATTGCTCCCAGCA TCCGTACCAAAACCATCAGTACCACGGCTACCACAGAAGCTGAACCGGAAGTTGACAACCTTCTAGTTTC AGATGCCACTCCAGACGGTTTCCGTCTGTCCTGGACTGCTGATGAAGGGATATTCGACAGTTTTGTTATC AGGATCAGAGATACCAAAAAGCAATCTGAGCCACAAGAAATATCCCTCCCTTCCCCCGAACGTACCAGGG ACATAACAGGTCTCAGAGAGGCCACTGAGTACGAAATTGAACTCTATGGAATAAGCCGCGGAAGGCGATC CCAGCCAGTCAGTGCCATAGCAACAACAGCCATGGGTTCTCCGAAGGAAATCATGTTCTCAGACATCACT GAAAATGCAGCCACAGTCAGCTGGAGGGCACCTACTGCTCAGGTGGAGAGTTTCCGGATCACTTATGTAC CTATGACAGGAGGTGCCCCGTCCATGGTGACTGTGGATGGAACAGATACTGAGACCAGGCTGGTGAAGCT TACCCCGGGTGTAGAGTACCGCGTCAGTGTGATTGCCATGAAGGGATTCGAAGAAAGCGATCCAGTCTCG GGGACTCTAATCACAGCTCTGGATGGTCCATCTGGTCTTCTGATAGCCAACATCACAGACTCAGAAGCCT TGGCCATGTGGCAGCCAGCCATTGCCACTGTGGACAGTTATGTCATCTCCTACACAGGGGAGAGAGTGCC AGAAGTTACACGCACAGTGTCTGGAAATACAGTGGAGTACGAGCTGCATGACCTGGAGCCTGCCACAGAG TACATCCTGAGTATCTTTGCAGAGAAAGGACAGCAGAAGAGCTCTACCATCGCCACCAAGTTTACCACAG ACCTCGATTCCCCAAGAGAATTTACAGCTACAGAGGTTCAGTCAGAAACTGCCCTCCTTACCTGGAGACC TCCCCGAGCATCGGTCACTGGATACCTCCTGGTCTATGAATCTGTGGATGGTACAGTCAAGGAAGTCATT GTGGGGCCTGACACCACCTCCTACAGCCTGGCAGACCTGAGCCCATCCACCCACTACTCAGCAAGGATCC AGGCATTGAGTGGGTCCCTGAGGAGCAAGCTGATCCAAACCATCTTCACAACAATTGGACTCCTGTACCC ATTCCCCAGGGACTGCTCTCAAGCAATGTTGAATGGTGATACTACCTCTGGCCTCTACACCATCTATATA AATGGTGACAAGACTCAAGCACTGGAAGTCTACTGTGATATGACCTCTGATGGAGGTGGATGGATTGTTT TCCTGAGACGCAAAAATGGACGTGAGGACTTCTATCGCAACTGGAAGGCCTATGCTGCTGGGTTTGGAGA CCGCAGAGAAGAATTTTGGCTTGGACTGGATAACCTGAGCAAAATCACAGCCCAAGGGCAGTATGAGCTC CGGGTGGACCTACAAGACCATGGGGAGTCTGCCTATGCTGTGTACGACAGATTCAGTGTTGGAGATGCCA AGAGTCGCTACAAGCTGAAGGTAGAAGGATACAGTGGAACAGCAGGTGACTCCATGAACTATCACAATGG TAGATCCTTCTCCACCTATGACAAGGACACAGACTCAGCCATCACCAACTGTGCCCTGTCCTACAAAGGA GCTTTCTGGTATAAGAACTGTCATCGTGTCAACCTGATGGGCAGATATGGGGACAATAACCACAGTCAGG GCGTTAACTGGTTCCATTGGAAGGGCCATGAGTACTCAATCCAGTTTGCTGAGATGAAACTGAGACCCAG CAACTTCCGAAATCTGGAAGGCAGGCGTAAGCGGGCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011607
Insert Size	6060 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011607.3, NP_035737.2
RefSeq Size	7100 bp
RefSeq ORF	6060 bp
Locus ID	21923
UniProt ID	Q80YX1
Gene Summary	Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth when provided to neurons in culture. May play a role in supporting the growth of epithelial tumors. Ligand for integrins ITGA8:ITGB1, ITGA9:ITGB1, ITGAV:ITGB3 and ITGAV:ITGB6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function]

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