Tnc (NM_011607) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI528729; C130033P17Rik; cytotactin; Hxb; Ten; tenascin-C; TN; TN-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR226329 representing NM_011607 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGTGACCTGGCTACTGCCAGGCATCTTTCTAGCTTTGTTTGCCCTCACTCCCGAAGGTGGGG TCCTCAAGAAAATCATCAGGCACAAGCGAGAGAGTGGGCTAAACATGACCCTGCCAGAAGAGAATCAGCC AGTGGTGTTCAACCATATCTACAACATCAAGTTGCCCATGGGTTCCCAATGCTCAGTGGATCTGGAGTCA GCGAGTGGGGAGAAAGACCTGACCCCCACGCCAGAGTCCAGTGGGAGCTTCCAGGAACATACAGTGGATG GGGAAAACCAAATTGTATTCACACACCGCATCAACATCCCCCGCCGGGCCTGTGGCTGTGCTGCAGCTCC AGATGTGAAGGAGCTCCTGAGCAGGCTGGAGGAGCTGGAGTTGCTGGTATCGTCTCTAAGGGAGCAGTGC ACCATGGGTACAGGCTGTTGCCTCCAACCTGCAGAAGGTCGTCTGGACACCAGGCCCTTCTGCAGCGGAC GGGGCAACTTCAGTGCAGAAGGCTGCGGCTGTGTCTGTGAACCAGGCTGGAAAGGCCCCAACTGCTCTGA GCCTGACTGCCCTGGGAACTGTAATCTCAGAGGCCAGTGCCTTGACGGCCAGTGTATCTGTGACGAGGGT TTCACTGGGGAAGACTGCAGCCAGCTGGCCTGTCCGAACGACTGCAATGACCAGGGCAGGTGTGTGAACG GGGTCTGTGTGTGCTTCGAAGGCTATGCCGGCCCTGACTGTGGCCTGGAAGTCTGCCCAGTGCCCTGCAG CGAGGAACACGGGATGTGTGTGGATGGCAGGTGTGTGTGCAAAGATGGCTTTGCGGGTGAGGACTGCAAC GAGCCCCTTTGCCTCAACAACTGCTACAATCGTGGGCGGTGTGTGGAGAACGAGTGTGTCTGCGACGAGG GCTTCACTGGCGAGGACTGCAGTGAGCTCATTTGCCCCAACGACTGCTTCGACCGCGGTCGCTGCATCAA TGGCACCTGCTACTGTGAAGAAGGTTTCACGGGCGAAGACTGCGGTGAGCTCACCTGCCCCAACGACTGT CAGGGCCGTGGCCAGTGTGAGGAGGGACAGTGTGTTTGCAACGAGGGCTTTGCAGGAGCAGACTGCAGTG AAAAGCGGTGTCCCGCCGATTGTCACCACCGTGGCCGCTGCCTCAACGGGCAGTGTGAGTGTGACGATGG GTTCACAGGGGCTGACTGTGGGGATCTCCAGTGTCCCAATGGCTGCAGTGGGCATGGCCGTTGTGTCAAC GGGCAGTGCGTGTGTGACGAGGGCTATACCGGAGAAGACTGTAGCCAGCGGCGATGCCCCAATGACTGTC ACAACCGGGGTCTCTGCGTACAGGGCAAGTGCATATGTGAACAAGGCTTCAAGGGCTTTGACTGTAGTGA GATGAGCTGTCCCAATGACTGCCACCAACATGGCCGCTGTGTGAATGGCATGTGTATCTGTGATGACGAC TACACTGGGGAAGACTGCAGAGACCGGCGCTGTCCCCGGGACTGTAGCCAGCGGGGGCGCTGTGTAGACG GACAGTGCATCTGTGAGGATGGCTTCACTGGCCCTGACTGTGCTGAGCTTTCCTGCCCCAGTGACTGCCA CGGCCATGGCCGCTGTGTGAATGGCCAATGCATCTGCCACGAGGGCTTCACTGGCAAAGACTGCAAAGAG CAAAGGTGCCCCAGTGATTGCCATGGCCAAGGCCGCTGTGAGGACGGCCAGTGTATCTGCCATGAGGGCT TTACGGGCCTGGACTGTGGGCAGCGCTCCTGTCCCAATGACTGCAGCAACCAAGGACAATGTGTGTCAGG CCGCTGCATCTGCAATGAAGGTTACACAGGGATAGACTGCTCTGAGGTGTCCCCTCCCAAAGACCTTATT GTGACAGAAGTAACAGAGGAGACTGTAAATCTGGCATGGGACAATGAGATGCGGGTCACTGAGTACCTCA TTATGTACACACCCACCCATGCTGATGGCCTAGAGATGCAGTTCCGTGTGCCTGGGGACCAGACATCCAC CACCATTCGGGAGCTGGAACCAGGGGTGGAGTACTTCATTCGTGTGTTCGCCATCTTGGAAAACAAGAGG AGCATCCCTGTCAGTGCCAGGGTTGCCACCTATTTGCCTGCACCTGAAGGTCTAAAATTCAAGTCTATCA AGGAGACATCTGTGGAAGTAGAGTGGGATCCTCTAGACATTGCTTTCGAAACATGGGAGATCATTTTCCG AAATATGAACAAAGAAGATGAGGGAGAGATCACAAAAAGTCTGAGGAGACCAGAGACCTCCTACCGCCAA ACTGGCCTTGCTCCTGGCCAAGAGTATGAAATATCTCTGCACATTGTGAAAAACAACACCCGAGGCCCCG GCTTGAAGAAAGTAACCACAACCCGCCTGGATGCCCCCAGCCATATTGAGGTGAAAGATGTCACAGACAC CACAGCACTGATCACCTGGTTCAAGCCCTTGGCTGAAATTGATAGCATTGAGCTCTCCTATGGCATCAAG GATGTACCTGGAGACCGTACCACCATCGACCTCACACACGAAGACAACCAGTACTCCATCGGGAACCTGA GACCTGACACGGAGTATGAGGTGTCCCTCATCTCCCGCAGAGTGGACATGGCAAGCAACCCTGCCAAAGA GACCTTCATCACAGGCCTGGATGCTCCCAGGAATCTCCGCCGTGTCTCACAGACAGACAACAGCATCACC TTGGAGTGGAGGAACGTCAAGGCAGACATCGACAGTTACAGAATTAAGTATGCCCCTATCTCTGGAGGTG ACCATGCTGAGATAGATGTTCCAAAGAGCCAGCAAGCCACAACCAAAACCACACTCACAGGTCTAAGGCC CGGAACTGAATATGGGATTGGTGTTTCTGCTGTCAAGGGAGACAAGGAGAGTGATCCAGCAACCATCAAT GCGGCCACAGAAATTGATGCACCCAAGGACTTACGGGTGTCTGAAACCACACAAGACAGTCTGACGTTTT TCTGGACGACACCCCTGGCCAAGTTTGATCGTTACCGCCTCAACTACAGCCTCCCCACAGGCCAGTCGAT GGAAGTCCAGCTGCCAAAGGATGCCACCTCCCATGTCCTGACAGACCTGGAGCCAGGGCAAGAATACACT GTTCTCCTCATTGCTGAGAAGGGCAGACACAAGAGCAAGCCTGCACGTGTGAAGGCATCCACGGAAGAAG TGCCTTCCCTGGAAAATCTCACTGTGACTGAGGCCGGCTGGGATGGCCTCAGACTCAACTGGACTGCAGA TGACCTGGCCTATGAGTACTTTGTCATTCAGGTACAGGAAGCCAACAATGTGGAGACTGCTCACAACTTC ACAGTACCTGGTAACCTCCGGGCTGCAGACATCCCAGGCCTCAAGGTTGCCACTTCTTATAGAGTCTCCA TCTATGGGGTAGCCCGGGGCTATAGAACACCAGTGCTCTCTGCCGAGACCTCCACAGGGACAACTCCCAA TCTGGGAGAGGTCACTGTGGCCGAGGTGGGCTGGGATGCCCTCACGCTCAACTGGACTGCTCCAGAAGGA GCCTATAAGAACTTTTTCATTCAGGTGCTAGAGGCTGACACGACCCAGACTGTCCAGAACCTCACAGTCC CAGGAGGACTGAGGTCAGTGGACCTGCCTGGGCTCAAAGCAGCCACCCGCTACTACATCACCCTTCGAGG GGTCACCCAGGACTTCGGCACGGCCCCTCTCTCTGTTGAGGTCTTGACAGAGGATCTCCCTCAGCTGGGA GGCTTGTCTGTGACTGAGGTCAGCTGGGACGGCCTCACACTCAACTGGACCACAGATGACCTGGCCTATA AGCACTTTGTCGTTCAGGTGCAGGAGGCCAACAATGTGGAGGCCGCTCAGAACCTCACGGTACCCGGCAG CCTCAGAGCTGTGGACATCCCAGGCCTCAAGGCCGACACCCCTTATAGAGTCTCCATCTACGGGGTCATC CAGGGCTATAGAACACCGATGCTCTCTACTGACGTCTCCACAGCCAGAGAACCTGAAATTGGAAACTTAA ATGTTTCTGATGTAACTCCTAAGAGCTTCAATCTCTCCTGGACAGCTACCGACGGGATCTTCGACATGTT TACTATTGAAATTATTGATTCTAATAGGTTGCTGCAAACAGCAGAACATAATATATCTGGTGCTGAACGG ACTGCCCACATCTCAGGGCTTCCACCTAGTACTGATTTCATTGTCTACCTCTCTGGAATTGCTCCCAGCA TCCGTACCAAAACCATCAGTACCACGGCTACCACAGAAGCTGAACCGGAAGTTGACAACCTTCTAGTTTC AGATGCCACTCCAGACGGTTTCCGTCTGTCCTGGACTGCTGATGAAGGGATATTCGACAGTTTTGTTATC AGGATCAGAGATACCAAAAAGCAATCTGAGCCACAAGAAATATCCCTCCCTTCCCCCGAACGTACCAGGG ACATAACAGGTCTCAGAGAGGCCACTGAGTACGAAATTGAACTCTATGGAATAAGCCGCGGAAGGCGATC CCAGCCAGTCAGTGCCATAGCAACAACAGCCATGGGTTCTCCGAAGGAAATCATGTTCTCAGACATCACT GAAAATGCAGCCACAGTCAGCTGGAGGGCACCTACTGCTCAGGTGGAGAGTTTCCGGATCACTTATGTAC CTATGACAGGAGGTGCCCCGTCCATGGTGACTGTGGATGGAACAGATACTGAGACCAGGCTGGTGAAGCT TACCCCGGGTGTAGAGTACCGCGTCAGTGTGATTGCCATGAAGGGATTCGAAGAAAGCGATCCAGTCTCG GGGACTCTAATCACAGCTCTGGATGGTCCATCTGGTCTTCTGATAGCCAACATCACAGACTCAGAAGCCT TGGCCATGTGGCAGCCAGCCATTGCCACTGTGGACAGTTATGTCATCTCCTACACAGGGGAGAGAGTGCC AGAAGTTACACGCACAGTGTCTGGAAATACAGTGGAGTACGAGCTGCATGACCTGGAGCCTGCCACAGAG TACATCCTGAGTATCTTTGCAGAGAAAGGACAGCAGAAGAGCTCTACCATCGCCACCAAGTTTACCACAG ACCTCGATTCCCCAAGAGAATTTACAGCTACAGAGGTTCAGTCAGAAACTGCCCTCCTTACCTGGAGACC TCCCCGAGCATCGGTCACTGGATACCTCCTGGTCTATGAATCTGTGGATGGTACAGTCAAGGAAGTCATT GTGGGGCCTGACACCACCTCCTACAGCCTGGCAGACCTGAGCCCATCCACCCACTACTCAGCAAGGATCC AGGCATTGAGTGGGTCCCTGAGGAGCAAGCTGATCCAAACCATCTTCACAACAATTGGACTCCTGTACCC ATTCCCCAGGGACTGCTCTCAAGCAATGTTGAATGGTGATACTACCTCTGGCCTCTACACCATCTATATA AATGGTGACAAGACTCAAGCACTGGAAGTCTACTGTGATATGACCTCTGATGGAGGTGGATGGATTGTTT TCCTGAGACGCAAAAATGGACGTGAGGACTTCTATCGCAACTGGAAGGCCTATGCTGCTGGGTTTGGAGA CCGCAGAGAAGAATTTTGGCTTGGACTGGATAACCTGAGCAAAATCACAGCCCAAGGGCAGTATGAGCTC CGGGTGGACCTACAAGACCATGGGGAGTCTGCCTATGCTGTGTACGACAGATTCAGTGTTGGAGATGCCA AGAGTCGCTACAAGCTGAAGGTAGAAGGATACAGTGGAACAGCAGGTGACTCCATGAACTATCACAATGG TAGATCCTTCTCCACCTATGACAAGGACACAGACTCAGCCATCACCAACTGTGCCCTGTCCTACAAAGGA GCTTTCTGGTATAAGAACTGTCATCGTGTCAACCTGATGGGCAGATATGGGGACAATAACCACAGTCAGG GCGTTAACTGGTTCCATTGGAAGGGCCATGAGTACTCAATCCAGTTTGCTGAGATGAAACTGAGACCCAG CAACTTCCGAAATCTGGAAGGCAGGCGTAAGCGGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR226329 representing NM_011607 Red=Cloning site Green=Tags(s) MGAVTWLLPGIFLALFALTPEGGVLKKIIRHKRESGLNMTLPEENQPVVFNHIYNIKLPMGSQCSVDLES ASGEKDLTPTPESSGSFQEHTVDGENQIVFTHRINIPRRACGCAAAPDVKELLSRLEELELLVSSLREQC TMGTGCCLQPAEGRLDTRPFCSGRGNFSAEGCGCVCEPGWKGPNCSEPDCPGNCNLRGQCLDGQCICDEG FTGEDCSQLACPNDCNDQGRCVNGVCVCFEGYAGPDCGLEVCPVPCSEEHGMCVDGRCVCKDGFAGEDCN EPLCLNNCYNRGRCVENECVCDEGFTGEDCSELICPNDCFDRGRCINGTCYCEEGFTGEDCGELTCPNDC QGRGQCEEGQCVCNEGFAGADCSEKRCPADCHHRGRCLNGQCECDDGFTGADCGDLQCPNGCSGHGRCVN GQCVCDEGYTGEDCSQRRCPNDCHNRGLCVQGKCICEQGFKGFDCSEMSCPNDCHQHGRCVNGMCICDDD YTGEDCRDRRCPRDCSQRGRCVDGQCICEDGFTGPDCAELSCPSDCHGHGRCVNGQCICHEGFTGKDCKE QRCPSDCHGQGRCEDGQCICHEGFTGLDCGQRSCPNDCSNQGQCVSGRCICNEGYTGIDCSEVSPPKDLI VTEVTEETVNLAWDNEMRVTEYLIMYTPTHADGLEMQFRVPGDQTSTTIRELEPGVEYFIRVFAILENKR SIPVSARVATYLPAPEGLKFKSIKETSVEVEWDPLDIAFETWEIIFRNMNKEDEGEITKSLRRPETSYRQ TGLAPGQEYEISLHIVKNNTRGPGLKKVTTTRLDAPSHIEVKDVTDTTALITWFKPLAEIDSIELSYGIK DVPGDRTTIDLTHEDNQYSIGNLRPDTEYEVSLISRRVDMASNPAKETFITGLDAPRNLRRVSQTDNSIT LEWRNVKADIDSYRIKYAPISGGDHAEIDVPKSQQATTKTTLTGLRPGTEYGIGVSAVKGDKESDPATIN AATEIDAPKDLRVSETTQDSLTFFWTTPLAKFDRYRLNYSLPTGQSMEVQLPKDATSHVLTDLEPGQEYT VLLIAEKGRHKSKPARVKASTEEVPSLENLTVTEAGWDGLRLNWTADDLAYEYFVIQVQEANNVETAHNF TVPGNLRAADIPGLKVATSYRVSIYGVARGYRTPVLSAETSTGTTPNLGEVTVAEVGWDALTLNWTAPEG AYKNFFIQVLEADTTQTVQNLTVPGGLRSVDLPGLKAATRYYITLRGVTQDFGTAPLSVEVLTEDLPQLG GLSVTEVSWDGLTLNWTTDDLAYKHFVVQVQEANNVEAAQNLTVPGSLRAVDIPGLKADTPYRVSIYGVI QGYRTPMLSTDVSTAREPEIGNLNVSDVTPKSFNLSWTATDGIFDMFTIEIIDSNRLLQTAEHNISGAER TAHISGLPPSTDFIVYLSGIAPSIRTKTISTTATTEAEPEVDNLLVSDATPDGFRLSWTADEGIFDSFVI RIRDTKKQSEPQEISLPSPERTRDITGLREATEYEIELYGISRGRRSQPVSAIATTAMGSPKEIMFSDIT ENAATVSWRAPTAQVESFRITYVPMTGGAPSMVTVDGTDTETRLVKLTPGVEYRVSVIAMKGFEESDPVS GTLITALDGPSGLLIANITDSEALAMWQPAIATVDSYVISYTGERVPEVTRTVSGNTVEYELHDLEPATE YILSIFAEKGQQKSSTIATKFTTDLDSPREFTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVI VGPDTTSYSLADLSPSTHYSARIQALSGSLRSKLIQTIFTTIGLLYPFPRDCSQAMLNGDTTSGLYTIYI NGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYEL RVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKG AFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSNFRNLEGRRKRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_011607
ORF Size	6057 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011607.3, NP_035737.2
RefSeq Size	6834 bp
RefSeq ORF	6060 bp
Locus ID	21923
UniProt ID	Q80YX1
MW	222.3 kDa
Gene Summary	Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth when provided to neurons in culture. May play a role in supporting the growth of epithelial tumors. Ligand for integrins ITGA8:ITGB1, ITGA9:ITGB1, ITGAV:ITGB3 and ITGAV:ITGB6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function]

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