Plce1 (NM_019588) Mouse Untagged Clone
CAT#: MC225255
Plce1 (untagged) - Mouse phospholipase C, epsilon 1 (Plce1), (10ug)
CNY 36,480.00
货期*
12周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4933403A21Rik; mKIAA1516; Plce; PLCepsilon |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC225255 representing NM_019588
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCGGAAGAAATGGCCGCTTCTGTCCTCATCCCTGTGACTCAAAGGAAAGTGGCTTCTGCCCAGT CGGTGGCAGAAGAGAGGAGTGTAAAGGTCTCAGATGCGGGGATTCCTCGGGCACGTGCTGGCAGGCAGGG TGCGCTCATCCCTCCTACCATCTCACAGTGGAACAAACATAAGGAAGAGTCGTCTAGAAGTGATTTGTCC AAGGTCTTCTCAATAGCGAGGGGGGAACTAGTCTGCGATGAGAATTCCAACGAAGAGGGCTGGGAGGAAA ACGCACCGGACTCCCCGGAAAACCACGCGATGAACGGCAACAGTTTAGTGCAAAGCCACCAGCACCAGTT TCCCCGGAGCCAGCTTTGTGAAGCCCGTGACTCTGTCACAGAAGACCCGTGTTTGCAGCCTGGAATTCCT TCTCCCCTGGAAAGGAAGGTGCTCCCTGGAATTCAACTGGAGATGGAGGACTCTCCCATGGACGTGAGCC CTGCGGGAAGTCAGCCTAGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACACGGCGGTATTTCA CTTCCATTATGAAGCTGACAGGACAATGTCGGATGCTTTTCATACCCTGTCAGAAAACCTGATTTTGGAT GACTGTGCAAATTGTGTTACTCTTCCTGGGGGGCAGCAAAATAAAAATTGCATGGCGTATGCTTGCAAAC TGGTGGAGTTGACAAGAACCTGTGGAAGTAAGAATGGGCAAGTCCAGTGTGAGCACTGTACCTCGCTGCG CGATGAATACCTGTGCTTCGAAAGCTCCTGCTCAAAGGCCGATGAGGTCTGCTCAGGTGGTGGCTTTTGC GAGGACGGCTTTGCTCACGGTCCTGCTGCCAAGACTTTTCTGAACCCTCTGGAGGATTTCTCTGACAATT GTGAAGACGTAGACGATTTTTTTAAAAGCAAAAAGGAGCGGTCCACGTTGTTAGTCCGAAGGTTTTGTAA AAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACCAGAGCCATCATGAGAACTCTGCCTTCTGGC TGTATTGGGCCAGCTGCTTGGAATTACGTTGATCAGAAGAAAGCTGGTCTCCTATGGCCTTGTGGGAATG TAATGGGAACTCTGTCAGCAATGGACATAAGGCAAAGTGGGAGCCAACGTCTGTCTGAAGCCCAGTGGTG CCTGATCTACAGTGCCGTGAGAAGAGGGGAAGAAATAGAAGATACGGTTGGATCTCTTCTCCATTGCTCC ACACAGCTCCCAAACTCTGAGACAGCCCATGGAAGGATTGAAGATGGTCCCTGCTTAAAGCAGTGTGTAC GAGACACTGAATGTGAATTCCGTGCTACCCTCCAAAGGACATCCATAGCTCAGTACATCACCGGTTCTCT TCTAGAGGCAACTACATCTTTAGGAGCAAGAAGTGGCCTTCTCAGTTCCTTCGGAGGATCCACTGGACGA ATAATGCTGAAAGAACGCCAACTGGGCACCTCTATGGCCAATTCCAATCCTGTTCCTTCAAGTTCAGCTG GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC AGAGCAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGCTTCTTAACCCGGGACTTG AGTTCACCTGAATGCCAGAGATCACTGCCCCGTCTCAAAGCATCCATCTCAGAATCCATCCTCACGTCTC AAAGTGGAGAACACAATGCCCTTGAAGACCTTGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG GCTGATCCTCACAGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA TGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC TAAAGATGTGGCAGTTCATGGACCAGTCTGACATTGAGACCATGCGGAGCCTGAAGGATGCCATGGCCCA GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTC CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT GCCCACGGTACAGCTCCCAAGAAGAAGCTCTGGAGTTTGTAGCCGATTACAGTGGACAAGATAACTTCTT ACAACGAGTGGGACAGAATGGCTTAAAGAATTCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGGTC ATCCGGAGCTGCAGCCGAAGTTTGGAGATGGAGGAGGAAGACAGTGCCAGTGAAGGGAGCGGCTCTAGGA AAAACTCCCTGAAGGACAAAGCCCGATGGCAGTTTATAATTGGAGATTTGCTGGATTCAGAAAATGACAT CTTTGAGAAGTCCAAAGAGTGTGACCCCCATGGATCCGAGGAGTCACAGAAAGCCTTTGACCATGGGACA GAGCTTATCCCATGGTACGTGCTGTCCATCCAAGCAGATGTGCACCAGTTCCTATTGCAGGGGGCCACAG TCATCCACTATGACCAGGACACACATCTCTCGGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGCAC GTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCAGGCGCCAGACCCAAGCTCGGTGTGCTTAGTAAC ATGGCCGAGCCTGGCAAGTTCCCATCCCCAGGCAACGCCGGGGTGAGCGGCCTGGCAGAGGGAATTCTGG ATCTGTTCTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA CAAACTTAGTAGCATGCTTCTATCGGAGACGGGGGTCACACTGCTCTATGGGCTTCAGACCACGGACAAT AGATTACTGCACTTTGTGGCGCCAAAGCACACGGCTGAGATGCTTTTCAGTGGATTGCTGGAACTCACTA CAGCTGTGAGAAAGATAAGGAGGTTCCCTGACCAACGACAGCAGTGGCTTCGGAAACAGTATGTCAGCCT CTATCAGGAGGATGGTCGGTATGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGCGG TGGAGCACTAGAAACCCCAGCCCTGGAATGTCAGCAAAGAATGCTGAGAAGCCCAACATGCAGAGAAACA ACACCCTGGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGAGAGAGTGGAGAGGTGAC GGACGATGAGATGGCTACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT CAAGACGTTAACGAACAAGAAGAGTCGGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA GAAGAGCCTACTCTTTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCCATCAGTGC CTGGAGCAGCAGCAGCTGGCATGGGCGAATCCGAGGAGGAATGCAAGGCTTCCAGAGCTTCATGGTCTCA GACAGTAACATGAGTTTCGTTGAATTCGTCGAGCTGTTCAAATCATTCAGCATCAGGAGCCGCAAGGACT TGAAGGACATCTTTGACATCTACTCTGTACCCTGCAATCGGTCTGCCTCGGAGTCAGCCCCACTCTACAC CAACCTGACAATCGAAGAAAATACCAGCGATCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC GACCTGGGGCTGTTCATTAAAAGTAAACAGCAGCTTTCTGACAACCAGAGGCAGATATCTGATGCTATCG CTGCTGCAAGCATCGTGACCAATGGTACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT ACTCCAGCTCAATGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTGAGCATC ATCCAGAAATTTGAGCCTAGCGTCAGCATGTGTCATCAAGGCCTACTGTCATTTGAAGGGTTTGCAAGGT TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGATGAGTCACGGGAGAACAAGAAAGAACTGCAGCT ACCCCTCTCCTACTATTATATAGAATCTTCACACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG TCCTCTGTGGAGCTCTATAGCCAGGTCCTCCTGCAAGGATGTAGAAGCATAGAGCTGGACTGCTGGGACG GGGATGATGGGATGCCCATCATCTATCATGGGCATACACTGACAACCAAGATCCCCTTCAAGGAAGTGGT GGAAGCCATCGATCGCAGTGCCTTCATCACCTCAGACCTGCCAATCATCATATCCATTGAGAACCATTGT TCATTGCCTCAACAACGAAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT TCTTATTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGATCAACTTAGAAGGAAAGTACT CCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTAAAACAAAAGGCTCATCAGTTA GCATCCATGCAAGCACAGGCTTTCACCGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG AAGATGAGGAGGATGAATATGATTACGATTACGAGTCCCTGTCCGATGACAATATTCTGGAAGACAGACC TGAGAACAAATCATGCGCTGACAAGCTTCAGTTTGAGTACAATGAAGAGGTCCCCAAGAGGATAAAGAAA GCAGACAACTCCTCTGGCAATAAGGGAAAGGTGTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC AGAACAAAAAGGAAAGCAGGCAGATTGCACCGGAGCTTTCTGACCTCGTCATTTATTGCCAGGCAGTAAA ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTAGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC ATTTTTGGCAACAATCCTGGCAGAATGAGCCCCGGGGAGACAGCGCCATTTAACAGAACATCTGGAAAAG GTTCCTGTGAAGGGATGCGACACACCTGGGAGGAATCTTCTCCACTCAGCCCAAGCACATCCCTCAGCGC TATCATCAGGACTCCTAAATGCTATCACATCTCCTCACTGAACGAGAACGCCGCCAAACGTCTGTGCCGC AGGGGTTCTCAGAAACTGATCCAGCACACCGCTTATCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAA TCGACTCCTCCAATCCCAACCCCATCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCA AACCGATGACCTTCCTTTACATTTAAACGCCGCCATGTTTGAGGCGAATGGTGGGTGTGGTTATGTTTTG AAGCCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTACCAGAAGTTTTCTCCACTGGAAAGAGACCTAG ACAACCTGGATCCTGCTATCTATTCTTTAACTATCATCTCTGGCCAGAATGTGTGCCCTAGTAACAGCAC CGGAAGTCCATGCATTGAAGTCGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCC ATCCATCGAAACACTCTAAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTG TATTTCTTCGTTTTGCAGTCGTGGAAAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAG AGCTTTGAAACGAGGATATCGACATCTTCAGCTGCGGAACCTCCACAATGAAATCTTGGAAATCTCTAGC TTATTCATAAACAGCAGAAGGATGGAAGAAAACCCCTCTGGCAGTTCCATGCCAGCCTCGTTGATGTTTA ATACTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTT TGCTGTTTTCACTATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGGTTTTGGCAGTTGACCAA GACACCAAATGTACTGCCACAGACTATTTTTTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATG AATGCAGGAAACAACCATTCCAGAGAGCTGTGGGTCCAGAAGAAGATATTGTGCAGATTTTAAACAGTTG GTTCCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAGGAAACTCTAGAAGAGAAAAGC ATTGTCTTCGACGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTGCAAGTGCATGATG TCTCTCCAGAGCAACCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTAATTCAGCA GACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAACCCATGTGACTATGTGCTTTTG GAAGAGGTGCTGAAAGATGCAGCTAATAAGAAGTCTTCTACCCCAAAGTCCTCGCAGAGGATCCTCTTGG ACCAAGAATGTGTGTTTCAAGCGCAAAGCAAGTGGAAGGGTGCGGGAAAGTTCATACTTAAGCTAAAGGA GCAGGTGCAGGCATCCCGAGAAGACAAAAGGAGGGGTATCTCCTTTGCAAGTGAACTCAAGAAGCTCACT AAGTCCACTAAGCAGTCTCGAGGGCTCCCATCACCTCCTCAGCTTGTGGCCTCAGAAAGTGTTCAAAGCA AGGAGGAGAAACCTGTGGGTGCCTTGTCCTCTAGTGACACAGTGGGTTACCAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_019588 |
Insert Size | 6849 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019588.2, NP_062534.2 |
RefSeq Size | 9525 bp |
RefSeq ORF | 6849 bp |
Locus ID | 74055 |
UniProt ID | Q8K4S1 |
Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.[UniProtKB/Swiss-Prot Function] |
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