Plce1 (NM_019588) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933403A21Rik; mKIAA1516; Plce; PLCepsilon
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225255 representing NM_019588 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCGGAAGAAATGGCCGCTTCTGTCCTCATCCCTGTGACTCAAAGGAAAGTGGCTTCTGCCCAGT CGGTGGCAGAAGAGAGGAGTGTAAAGGTCTCAGATGCGGGGATTCCTCGGGCACGTGCTGGCAGGCAGGG TGCGCTCATCCCTCCTACCATCTCACAGTGGAACAAACATAAGGAAGAGTCGTCTAGAAGTGATTTGTCC AAGGTCTTCTCAATAGCGAGGGGGGAACTAGTCTGCGATGAGAATTCCAACGAAGAGGGCTGGGAGGAAA ACGCACCGGACTCCCCGGAAAACCACGCGATGAACGGCAACAGTTTAGTGCAAAGCCACCAGCACCAGTT TCCCCGGAGCCAGCTTTGTGAAGCCCGTGACTCTGTCACAGAAGACCCGTGTTTGCAGCCTGGAATTCCT TCTCCCCTGGAAAGGAAGGTGCTCCCTGGAATTCAACTGGAGATGGAGGACTCTCCCATGGACGTGAGCC CTGCGGGAAGTCAGCCTAGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACACGGCGGTATTTCA CTTCCATTATGAAGCTGACAGGACAATGTCGGATGCTTTTCATACCCTGTCAGAAAACCTGATTTTGGAT GACTGTGCAAATTGTGTTACTCTTCCTGGGGGGCAGCAAAATAAAAATTGCATGGCGTATGCTTGCAAAC TGGTGGAGTTGACAAGAACCTGTGGAAGTAAGAATGGGCAAGTCCAGTGTGAGCACTGTACCTCGCTGCG CGATGAATACCTGTGCTTCGAAAGCTCCTGCTCAAAGGCCGATGAGGTCTGCTCAGGTGGTGGCTTTTGC GAGGACGGCTTTGCTCACGGTCCTGCTGCCAAGACTTTTCTGAACCCTCTGGAGGATTTCTCTGACAATT GTGAAGACGTAGACGATTTTTTTAAAAGCAAAAAGGAGCGGTCCACGTTGTTAGTCCGAAGGTTTTGTAA AAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACCAGAGCCATCATGAGAACTCTGCCTTCTGGC TGTATTGGGCCAGCTGCTTGGAATTACGTTGATCAGAAGAAAGCTGGTCTCCTATGGCCTTGTGGGAATG TAATGGGAACTCTGTCAGCAATGGACATAAGGCAAAGTGGGAGCCAACGTCTGTCTGAAGCCCAGTGGTG CCTGATCTACAGTGCCGTGAGAAGAGGGGAAGAAATAGAAGATACGGTTGGATCTCTTCTCCATTGCTCC ACACAGCTCCCAAACTCTGAGACAGCCCATGGAAGGATTGAAGATGGTCCCTGCTTAAAGCAGTGTGTAC GAGACACTGAATGTGAATTCCGTGCTACCCTCCAAAGGACATCCATAGCTCAGTACATCACCGGTTCTCT TCTAGAGGCAACTACATCTTTAGGAGCAAGAAGTGGCCTTCTCAGTTCCTTCGGAGGATCCACTGGACGA ATAATGCTGAAAGAACGCCAACTGGGCACCTCTATGGCCAATTCCAATCCTGTTCCTTCAAGTTCAGCTG GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC AGAGCAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGCTTCTTAACCCGGGACTTG AGTTCACCTGAATGCCAGAGATCACTGCCCCGTCTCAAAGCATCCATCTCAGAATCCATCCTCACGTCTC AAAGTGGAGAACACAATGCCCTTGAAGACCTTGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG GCTGATCCTCACAGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA TGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC TAAAGATGTGGCAGTTCATGGACCAGTCTGACATTGAGACCATGCGGAGCCTGAAGGATGCCATGGCCCA GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTC CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT GCCCACGGTACAGCTCCCAAGAAGAAGCTCTGGAGTTTGTAGCCGATTACAGTGGACAAGATAACTTCTT ACAACGAGTGGGACAGAATGGCTTAAAGAATTCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGGTC ATCCGGAGCTGCAGCCGAAGTTTGGAGATGGAGGAGGAAGACAGTGCCAGTGAAGGGAGCGGCTCTAGGA AAAACTCCCTGAAGGACAAAGCCCGATGGCAGTTTATAATTGGAGATTTGCTGGATTCAGAAAATGACAT CTTTGAGAAGTCCAAAGAGTGTGACCCCCATGGATCCGAGGAGTCACAGAAAGCCTTTGACCATGGGACA GAGCTTATCCCATGGTACGTGCTGTCCATCCAAGCAGATGTGCACCAGTTCCTATTGCAGGGGGCCACAG TCATCCACTATGACCAGGACACACATCTCTCGGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGCAC GTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCAGGCGCCAGACCCAAGCTCGGTGTGCTTAGTAAC ATGGCCGAGCCTGGCAAGTTCCCATCCCCAGGCAACGCCGGGGTGAGCGGCCTGGCAGAGGGAATTCTGG ATCTGTTCTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA CAAACTTAGTAGCATGCTTCTATCGGAGACGGGGGTCACACTGCTCTATGGGCTTCAGACCACGGACAAT AGATTACTGCACTTTGTGGCGCCAAAGCACACGGCTGAGATGCTTTTCAGTGGATTGCTGGAACTCACTA CAGCTGTGAGAAAGATAAGGAGGTTCCCTGACCAACGACAGCAGTGGCTTCGGAAACAGTATGTCAGCCT CTATCAGGAGGATGGTCGGTATGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGCGG TGGAGCACTAGAAACCCCAGCCCTGGAATGTCAGCAAAGAATGCTGAGAAGCCCAACATGCAGAGAAACA ACACCCTGGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGAGAGAGTGGAGAGGTGAC GGACGATGAGATGGCTACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT CAAGACGTTAACGAACAAGAAGAGTCGGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA GAAGAGCCTACTCTTTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCCATCAGTGC CTGGAGCAGCAGCAGCTGGCATGGGCGAATCCGAGGAGGAATGCAAGGCTTCCAGAGCTTCATGGTCTCA GACAGTAACATGAGTTTCGTTGAATTCGTCGAGCTGTTCAAATCATTCAGCATCAGGAGCCGCAAGGACT TGAAGGACATCTTTGACATCTACTCTGTACCCTGCAATCGGTCTGCCTCGGAGTCAGCCCCACTCTACAC CAACCTGACAATCGAAGAAAATACCAGCGATCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC GACCTGGGGCTGTTCATTAAAAGTAAACAGCAGCTTTCTGACAACCAGAGGCAGATATCTGATGCTATCG CTGCTGCAAGCATCGTGACCAATGGTACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT ACTCCAGCTCAATGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTGAGCATC ATCCAGAAATTTGAGCCTAGCGTCAGCATGTGTCATCAAGGCCTACTGTCATTTGAAGGGTTTGCAAGGT TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGATGAGTCACGGGAGAACAAGAAAGAACTGCAGCT ACCCCTCTCCTACTATTATATAGAATCTTCACACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG TCCTCTGTGGAGCTCTATAGCCAGGTCCTCCTGCAAGGATGTAGAAGCATAGAGCTGGACTGCTGGGACG GGGATGATGGGATGCCCATCATCTATCATGGGCATACACTGACAACCAAGATCCCCTTCAAGGAAGTGGT GGAAGCCATCGATCGCAGTGCCTTCATCACCTCAGACCTGCCAATCATCATATCCATTGAGAACCATTGT TCATTGCCTCAACAACGAAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT TCTTATTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGATCAACTTAGAAGGAAAGTACT CCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTAAAACAAAAGGCTCATCAGTTA GCATCCATGCAAGCACAGGCTTTCACCGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG AAGATGAGGAGGATGAATATGATTACGATTACGAGTCCCTGTCCGATGACAATATTCTGGAAGACAGACC TGAGAACAAATCATGCGCTGACAAGCTTCAGTTTGAGTACAATGAAGAGGTCCCCAAGAGGATAAAGAAA GCAGACAACTCCTCTGGCAATAAGGGAAAGGTGTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC AGAACAAAAAGGAAAGCAGGCAGATTGCACCGGAGCTTTCTGACCTCGTCATTTATTGCCAGGCAGTAAA ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTAGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC ATTTTTGGCAACAATCCTGGCAGAATGAGCCCCGGGGAGACAGCGCCATTTAACAGAACATCTGGAAAAG GTTCCTGTGAAGGGATGCGACACACCTGGGAGGAATCTTCTCCACTCAGCCCAAGCACATCCCTCAGCGC TATCATCAGGACTCCTAAATGCTATCACATCTCCTCACTGAACGAGAACGCCGCCAAACGTCTGTGCCGC AGGGGTTCTCAGAAACTGATCCAGCACACCGCTTATCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAA TCGACTCCTCCAATCCCAACCCCATCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCA AACCGATGACCTTCCTTTACATTTAAACGCCGCCATGTTTGAGGCGAATGGTGGGTGTGGTTATGTTTTG AAGCCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTACCAGAAGTTTTCTCCACTGGAAAGAGACCTAG ACAACCTGGATCCTGCTATCTATTCTTTAACTATCATCTCTGGCCAGAATGTGTGCCCTAGTAACAGCAC CGGAAGTCCATGCATTGAAGTCGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCC ATCCATCGAAACACTCTAAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTG TATTTCTTCGTTTTGCAGTCGTGGAAAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAG AGCTTTGAAACGAGGATATCGACATCTTCAGCTGCGGAACCTCCACAATGAAATCTTGGAAATCTCTAGC TTATTCATAAACAGCAGAAGGATGGAAGAAAACCCCTCTGGCAGTTCCATGCCAGCCTCGTTGATGTTTA ATACTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTT TGCTGTTTTCACTATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGGTTTTGGCAGTTGACCAA GACACCAAATGTACTGCCACAGACTATTTTTTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATG AATGCAGGAAACAACCATTCCAGAGAGCTGTGGGTCCAGAAGAAGATATTGTGCAGATTTTAAACAGTTG GTTCCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAGGAAACTCTAGAAGAGAAAAGC ATTGTCTTCGACGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTGCAAGTGCATGATG TCTCTCCAGAGCAACCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTAATTCAGCA GACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAACCCATGTGACTATGTGCTTTTG GAAGAGGTGCTGAAAGATGCAGCTAATAAGAAGTCTTCTACCCCAAAGTCCTCGCAGAGGATCCTCTTGG ACCAAGAATGTGTGTTTCAAGCGCAAAGCAAGTGGAAGGGTGCGGGAAAGTTCATACTTAAGCTAAAGGA GCAGGTGCAGGCATCCCGAGAAGACAAAAGGAGGGGTATCTCCTTTGCAAGTGAACTCAAGAAGCTCACT AAGTCCACTAAGCAGTCTCGAGGGCTCCCATCACCTCCTCAGCTTGTGGCCTCAGAAAGTGTTCAAAGCA AGGAGGAGAAACCTGTGGGTGCCTTGTCCTCTAGTGACACAGTGGGTTACCAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019588
Insert Size	6849 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019588.2, NP_062534.2
RefSeq Size	9525 bp
RefSeq ORF	6849 bp
Locus ID	74055
UniProt ID	Q8K4S1
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.[UniProtKB/Swiss-Prot Function]

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