Arid1a (NM_001080819) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110030E03Rik; BAF250a; Osa1; Smarcf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225259 representing NM_001080819 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGCAGGTCGCCCCCGCCGCCGCCAGCAGCCTGGGCAACCCGCCGCCGCCGCCCTCGGAGCTGA AGAAAGCCGAGCAGCAACAGCGGGAGGAGGCGGGGGGCGAGGCGGCGGCGGCAGCGGCCGAGCGCGGGGA AATGAAGGCAGCCGCCGGGCAGGAGAGCGAGGGCCCCGCCGTGGGGCCGCCGCAGCCGCTGGGAAAGGAG CTGCAGGACGGGGCCGAGAGCAATGGGGGTGGCGGCGGCGGCGGAGCCGGCAGCGGCGGCGGGCCCGGCG CGGAGCCGGACCTGAAGAACTCGAACGGGAACGCGGGCCCTAGGCCCGCCCTGAACAATAACCTCCCGGA GCCGCCCGGCGGCGGCGGCGGCGGCGGCAGCAGCAGCAGCGACGGGGTGGGGGCGCCTCCTCACTCGGCC GCGGCCGCCCTGCCGCCCCCAGCCTACGGCTTCGGGCAAGCCTACGGCCGGAGCCCGTCTGCCGTCGCCG CCGCGGCGGCCGCCGTCTTCCACCAACAACATGGCGGACAACAAAGCCCTGGCCTGGCAGCGCTGCAGAG CGGCGGCGGCGGGGGCTTGGAGCCCTACGCCGGGCCCCAGCAGAACTCGCACGACCACGGCTTCCCCAAC CACCAGTACAACTCCTACTACCCCAACCGCAGCGCCTACCCCCCGCCTCCCCAGGCCTACGCGCTGAGCT CCCCGAGAGGTGGCACTCCGGGCTCCGGCGCGGCGGCGGCCGCCGGCTCCAAGCCGCCTCCCTCCTCCAG CGCCTCTGCCTCCTCGTCGTCTTCGTCCTTCGCACAGCAGCGCTTCGGGGCCATGGGGGGAGGCGGCCCC TCAGCGGCCGGCGGGGGAACTCCCCAGCCCACCGCCACCCCCACCCTCAACCAACTGCTCACGTCGCCCA GCTCGGCCCGTGGCTACCAGGGCTACCCCGGGGGCGACTACGGCGGCGGGCCCCAGGACGGGGGCGCGGG CAAAGGCCCGGCGGACATGGCCTCGCAGTGCTGGGGGGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCC GTCTCGGGAGGGGCCCAACAAAGGAGCCACCACGCGCCCATGAGCCCCGGGAGCAGCGGCGGCGGGGGGC AGCCGCTCGCCCGGACCCCTCAGTCATCCAGTCCAATGGATCAGATGGGAAAGATGAGACCTCAGCCGTA TGGTGGGACTAACCCATACTCGCAACAACAGGGACCTCCTTCAGGACCGCAACAAGGACATGGGTACCCA GGGCAGCCATATGGGTCCCAGACTCCACAGCGGTACCCCATGACCATGCAGGGCCGGGCTCAGAGTGCCA TGGGCAGCCTCTCTTATGCACAGCAGATTCCACCTTATGGCCAGCAAGGCCCCAGTGCGTATGGCCAGCA GGGCCAGACTCCATACTATAACCAGCAAAGTCCTCATCCCCAGCAGCAGCCACCTTACGCCCAGCAACCA CCATCCCAGACCCCTCATGCCCAGCCTTCGTATCAGCAGCAGCCGCAGACTCAGCAACCACAGCTTCAGT CCTCTCAGCCTCCATATTCCCAGCAGCCATCCCAGCCTCCACATCAGCAGTCCCCAACTCCATATCCCTC CCAGCAGTCCACCACACAACAGCATCCCCAGAGCCAGCCCCCCTACTCACAACCACAGGCACAGTCTCCC TACCAGCAGCAGCAACCTCAGCAGCCAGCATCCTCGTCGCTCTCCCAGCAGGCTGCATATCCTCAGCCCC AGCCTCAGCAGTCCCAGCAAACTGCCTATTCCCAGCAGCGCTTCCCTCCACCACAGGAGCTTTCTCAAGA TTCATTTGGGTCTCAGGCATCCTCAGCCCCCTCAATGACCTCCAGTAAGGGAGGGCAAGAAGATATGAAC CTGAGTCTTCAGTCAAGGCCCTCCAGCTTGCCTGATCTGTCTGGTTCAATCGATGATCTCCCCATGGGGA CAGAAGGAGCTCTGAGTCCTGGCGTGAGCACATCAGGGATTTCCAGCAGCCAAGGAGAGCAGAGCAATCC AGCTCAGTCTCCCTTTTCTCCTCACACCTCCCCTCACCTGCCTGGCATCCGAGGCCCGTCCCCGTCCCCT GTTGGCTCTCCTGCCAGTGTCGCGCAGTCTCGCTCAGGACCACTCTCGCCTGCTGCAGTGCCAGGCAACC AGATGCCACCTCGGCCACCCAGTGGCCAGTCAGACAGCATCATGCACCCTTCCATGAACCAATCAAGCAT TGCCCAAGATCGAGGTTATATGCAGAGGAACCCCCAGATGCCCCAGTACACTTCCCCTCAGCCTGGCTCG GCCTTATCCCCACGTCAGCCGTCTGGAGGACAGATGCACTCGGGCGTGGGCTCCTACCAGCAGAACTCCA TGGGGAGCTACGGCCCCCAGGGCAGTCAGTATGGCCCACAAGGAGGCTATCCTAGGCAGCCTAACTATAA TGCCTTGCCCAACGCCAACTACCCCAATGCAGGCATGGCCGGAAGTATGAACCCTATGGGTGCTGGAGGT CAGATGCATGGGCAGCCTGGAATCCCACCTTACGGCACACTCCCTCCAGGGAGAATGGCTCATGCGTCTA TGGGCAACAGGCCCTATGGCCCTAATATGGCCAATATGCCACCTCAGGTTGGGTCAGGGATGTGTCCTCC ACCAGGGGGAATGAACAGGAAAACTCAAGAGTCTGCTGTTGCCATGCATGTTGCTGCCAACTCTATCCAA AACAGGCCACCAGGCTACCCAAATATGAATCAAGGGGGCATGATGGGAACTGGACCTCCCTATGGACAGG GGATCAATAGTATGGCTGGCATGATCAACCCTCAGGGACCCCCATATCCTATGGGTGGAACCATGGCCAA CAATTCAGCAGGGATGGCAGCCAGCCCAGAGATGATGGGCCTTGGGGATGTTAAGTTAACTCCCGCCACA AAAATGAACAACAAGGCAGATGGAACACCCAAGACAGAATCCAAATCTAAGAAATCCAGTTCTTCTACCA CCACCAATGAGAAGATCACCAAATTGTATGAGTTGGGTGGTGAGCCCGAGAGGAAGATGTGGGTGGACCG GTACCTGGCCTTCACAGAGGAGAAGGCCATGGGCATGACAAATCTGCCTGCTGTGGGGAGGAAGCCTCTG GACCTCTATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGGTTGACTCAGGTCAACAAGAACAAAAAAT GGCGGGAACTTGCAACCAACCTCAATGTGGGTACATCAAGCAGTGCTGCCAGCTCACTGAAAAAGCAGTA TATCCAATGTCTCTATGCCTTTGAGTGCAAGATCGAGCGTGGAGAAGACCCTCCCCCCGATATCTTCGCA GCTGCTGACTCCAAGAAGTCCCAACCCAAGATCCAGCCCCCCTCTCCTGCGGGATCAGGGTCTATGCAGG GGCCACAAACTCCTCAGTCAACCAGCAGTTCTATGGCAGAAGGAGGAGACCTGAAGCCACCAACTCCAGC ATCCACACCACATAGTCAAATTCCCCCCTTACCAGGCATGAGCAGGAGCAACTCAGTCGGAATCCAGGAT GCCTTTCCTGATGGAAGTGACCCCACATTCCAGAAGCGGAATTCCATGACTCCAAACCCTGGGTACCAGC CCAGTATGAATACCTCTGACATGATGGGGCGCATGTCCTATGAGCCAAATAAGGATCCTTATGGCAGCAT GAGGAAAGCGCCAGGAAGTGATCCCTTCATGTCCTCAGGGCAGGGCCCCAATGGCGGGATGGGTGATCCC TACAGCCGTGCTGCTGGCCCTGGGCTGGGAAGTGTGGCGATGGGACCACGGCAGCACTATCCCTATGGAG GTCCTTACGACAGAGTGAGGACGGAGCCTGGAATCGGGCCTGAAGGAAATATGGGCACTGGAGCCCCTCA GCCAAATCTCATGCCTTCCACCCCAGATTCGGGGATGTATTCTCCTAGCCGCTACCCCCCGCAGCAGCAG CAGCAACAGCAGCAACAACATGATTCCTATGGCAATCAATTCTCTACCCAAGGCACCCCTTCCAGCAGCC CCTTCCCCAGCCAGCAGACCACAATGTATCAGCAGCAGCAGCAGAATTATAAGAGGCCAATGGATGGCAC ATATGGCCCCCCTGCCAAGCGGCATGAAGGGGAGATGTACAGTGTGCCGTACAGCGCTGGGCAAGGCCAG CCTCAACAGCAGCAGTTGCCTGCAGCTCAGTCCCAGCCTGCCAGCCAGCCACAAGCTGCCCAGCCTTCCC CTCAGCAGGACGTGTACAACCAGTACAGCAATGCCTACCCTGCCTCCGCCACCGCTGCTACTGATCGCCG ACCAGCAGGCGGCCCCCAGAACCAATTTCCATTCCAGTTTGGCCGAGACCGAGTCTCTGCACCTCCTGGT TCCAGTGCCCAGCAGAACATGCCACCACAAATGATGGGTGGCCCCATACAGGCATCAGCTGAGGTTGCTC AGCAGGGCACCATGTGGCAGGGGCGAAATGACATGACCTACAATTATGCCAACAGGCAGAACACAGGCTC TGCCACCCAGGGCCCTGCGTATCATGGTGTGAACCGAACAGATGAAATGCTCCACACAGATCAGAGGGCC AACCATGAAGGCCCATGGCCTTCCCATGGCACACGCCAGCCTCCGTATGGTCCTTCAGCCCCTGTTCCCC CCATGACAAGGCCCCCTCCATCTAACTACCAGCCCCCACCAAGCATGCCGAATCACATTCCTCAGGTATC CAGCCCCGCTCCCCTCCCCCGGCCCATGGAGAACCGTACTTCTCCTAGCAAGTCTCCATTCCTGCACTCT GGGATGAAAATGCAAAAGGCGGGTCCACCGGTGCCTGCTTCGCACATAGCGCCTACCCCTGTGCAGCCGC CTATGATTCGGCGGGATATCACCTTCCCACCTGGCTCTGTAGAGGCCACTCAGCCTGTGTTGAAGCAGAG AAGGCGGCTCACAATGAAAGACATTGGAACCCCGGAGGCATGGCGGGTAATGATGTCCCTCAAGTCCGGG CTCCTGGCAGAGAGCACGTGGGCGTTAGACACCATTAACATTCTACTGTATGATGACAACAGCATTATGA CCTTCAACCTCAGCCAGCTCCCAGGCTTGCTAGAGCTCCTTGTGGAATATTTCCGTAGATGCCTAATTGA AATCTTTGGCATTTTAAAGGAGTATGAGGTAGGGGACCCAGGACAGAGAACATTACTAGACCCTGGGAGA TTCACCAAGGTGTATAGTCCAGCCCATACAGAGGAAGAAGAGGAAGAACACCTTGATCCTAAACTGGAGG AGGAAGAGGAAGAAGGGGTTGGAAATGATGAGGAGATGGCCTTTTTGGGCAAGGACAAGCCATCTTCAGA GAATAATGAGGAGAAGCTAGTCAGTAAGTTTGACAAGCTTCCGGTAAAGATCGTGCAGAGGAATGACCCA TTTGTGGTGGACTGCTCAGATAAGCTTGGGCGCGTGCAGGAGTTTGACAGTGGCCTGCTACACTGGCGGA TTGGTGGTGGGGATACCACTGAGCATATCCAGACCCACTTTGAGAGCAAGATAGAGCTGCTGCCTTCCCG GCCTTATGTGCCCTGCCCAACGCCCCCTCGGAAACACCTCACAACAGTAGAGGGCACACCAGGGACAACG GAGCAGGAGGGCCCCCCGCCCGATGGCCTTCCAGAGAAAAGGATCACAGCCACCATGGATGACATGTTGT CTACCCGGTCTAGCACATTGACTGATGAGGGGGCAAAGAGTGCAGAGGCCACCAAGGAAAGCAGCAAGTT TCCATTTGGCATTAGCCCAGCACAGAGCCACCGGAACATCAAAATTTTAGAGGATGAACCCCATAGTAAG GATGAGACCCCACTGTGTACCCTTCTGGACTGGCAGGATTCCCTTGCTAAGCGCTGTGTCTGTGTCTCCA ATACCATCCGGAGCCTGTCGTTTGTGCCAGGCAACGACTTTGAGATGTCCAAACACCCAGGGCTGCTGCT TATCCTGGGCAAGCTGATCCTGCTGCACCACAAGCACCCAGAGCGGAAGCAGGCACCACTAACTTATGAG AAGGAGGAGGAACAGGACCAAGGGGTGAGCTGTGACAAAGTGGAGTGGTGGTGGGACTGCTTGGAGATGC TCCGAGAAAACACGCTGGTCACCCTCGCCAACATCTCGGGGCAATTGGACCTATCCCCATATCCTGAGAG CATCTGCCTGCCTGTCCTGGACGGACTCCTACACTGGGCAGTTTGCCCTTCAGCTGAAGCCCAGGACCCC TTCTCAACCCTAGGCCCCAATGCCGTCCTCTCCCCCCAGAGATTGGTCTTGGAAACCCTCAGCAAACTCA GCATCCAGGACAACAATGTGGACCTGATCCTGGCCACTCCCCCTTTTAGCCGCCTGGAGAAGTTGTATAG TACCATGGTGCGCTTCCTCAGTGACCGAAAGAACCCAGTGTGCCGGGAGATGGCCGTGGTACTGCTGGCA AATCTGGCCCAGGGGGACAGCCTGGCAGCCCGGGCCATTGCAGTGCAGAAGGGCAGCATCGGCAACCTCC TGGGTTTCCTGGAGGACAGCCTTGCTGCCACACAGTTCCAGCAGAGCCAGGCAAGCCTCCTGCATATGCA GAATCCACCCTTTGAACCAACTAGTGTGGACATGATGCGGCGGGCTGCCCGAGCACTGCTTGCCCTGGCC AAGGTGGATGAGAACCACTCAGAGTTCACTCTGTATGAGTCACGGCTGTTGGACATCTCCGTGTCACCAC TGATGAACTCATTGGTTTCACAAGTCATTTGTGATGTACTGTTTTTGATTGGCCAGTCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001080819
Insert Size	6852 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080819.1, NP_001074288.1
RefSeq Size	8175 bp
RefSeq ORF	6852 bp
Locus ID	93760
UniProt ID	A2BH40
Gene Summary	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523).[UniProtKB/Swiss-Prot Function]

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