F8 (NM_007977) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cf-8; Cf8; FVIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225271 representing NM_007977 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAATAGCACTCTTCGCTTGCTTCTTTCTGAGCCTTTTCAATTTCTGCTCTAGTGCCATCAGAAGAT ACTACCTTGGTGCAGTGGAATTGTCCTGGAACTATATTCAGAGTGATCTGCTCAGTGTGCTGCATACAGA CTCAAGATTTCTTCCTAGAATGTCAACATCTTTTCCATTCAACACCTCCATCATGTATAAAAAGACTGTG TTTGTAGAGTACAAGGACCAGCTTTTCAACATTGCCAAGCCCAGGCCACCCTGGATGGGTTTGCTAGGTC CTACCATTTGGACTGAGGTTCATGACACAGTGGTCATTACACTTAAAAACATGGCTTCTCATCCTGTCAG TCTTCATGCTGTTGGTGTGTCCTACTGGAAAGCTTCTGAGGGAGATGAATATGAAGATCAGACAAGCCAA ATGGAGAAGGAAGATGATAAAGTTTTCCCTGGTGAAAGTCATACTTATGTTTGGCAAGTCCTGAAAGAGA ATGGTCCAATGGCCTCTGACCCTCCATGTCTCACTTACTCATATATGTCTCATGTGGATCTGGTGAAAGA TTTGAATTCAGGCCTCATTGGAGCTCTGCTAGTATGTAAAGAAGGCAGTCTCTCCAAAGAAAGAACACAG ATGTTGTACCAATTTGTACTGCTTTTTGCTGTATTTGATGAAGGGAAGAGCTGGCACTCAGAAACAAACG ACTCTTATACACAGTCTATGGATTCTGCATCTGCTAGAGACTGGCCTAAAATGCACACAGTCAATGGCTA TGTAAACAGGTCTCTTCCAGGTCTGATTGGATGCCATAGGAAATCAGTCTACTGGCACGTGATTGGAATG GGCACCACTCCTGAAATACACTCAATATTCCTCGAAGGTCACACATTTTTTGTGAGGAACCACCGTCAAG CTTCATTGGAGATATCACCAATAACTTTCCTTACTGCTCAAACACTCTTGATAGATCTTGGGCAGTTCCT ACTATTTTGTCATATCTCTTCCCATAAACATGATGGCATGGAAGCTTATGTCAAAGTAGATAGCTGCCCT GAGGAATCCCAATGGCAAAAGAAAAATAATAATGAGGAAATGGAAGATTATGATGATGATCTTTATTCAG AAATGGATATGTTCACATTGGATTATGACAGCTCTCCTTTTATCCAAATTCGCTCGGTTGCTAAAAAGTA CCCTAAAACTTGGATACATTATATTTCTGCTGAGGAGGAAGACTGGGACTATGCACCTTCAGTTCCTACC TCGGATAATGGAAGTTATAAAAGCCAGTATCTGAGCAATGGTCCTCATCGGATTGGTAGGAAATATAAAA AAGTCAGATTTATAGCATACACAGATGAAACCTTTAAGACTCGTGAAACTATTCAGCATGAATCAGGACT CTTGGGACCTTTACTTTATGGAGAAGTTGGAGACACACTGTTGATTATTTTTAAGAATCAAGCAAGCCGA CCATATAACATTTACCCTCATGGAATCACTGATGTCAGTCCTCTACATGCAAGGAGATTGCCAAGAGGTA TAAAGCACGTGAAGGATTTGCCAATTCATCCAGGAGAGATATTCAAGTACAAGTGGACAGTTACAGTAGA AGATGGACCAACTAAATCAGATCCACGGTGCCTGACCCGCTATTATTCAAGTTTCATTAACCCTGAGAGA GATCTAGCTTCAGGACTGATTGGCCCTCTTCTCATCTGCTACAAAGAATCTGTAGATCAAAGGGGAAACC AGATGATGTCAGACAAAAGAAATGTCATCCTGTTTTCTATATTTGATGAGAACCAAAGCTGGTACATCAC AGAGAACATGCAACGCTTCCTCCCCAATGCAGCTAAAACACAGCCCCAGGACCCTGGGTTCCAGGCCTCC AACATCATGCACAGCATCAATGGCTATGTTTTTGATAGCTTGGAGTTGACAGTTTGTTTGCATGAGGTGG CATACTGGCACATTCTCAGTGTTGGAGCACAGACAGACTTCTTATCTATCTTCTTCTCTGGATATACTTT CAAACACAAAATGGTCTATGAAGATACACTTACCCTGTTCCCATTCTCAGGAGAAACTGTCTTTATGTCG ATGGAAAACCCAGGTCTATGGGTCTTGGGGTGTCATAATTCAGACTTTCGGAAGAGAGGTATGACAGCAT TGCTGAAAGTTTCTAGTTGTGACAAGAGCACTAGTGATTATTATGAAGAAATATATGAAGATATTCCAAC ACAGTTGGTGAATGAGAACAATGTCATTGATCCCAGAAGCTTCTTCCAGAATACAAATCATCCTAATACT AGGAAAAAGAAATTCAAAGATTCCACAATTCCAAAAAATGATATGGAGAAGATTGAGCCTCAGTTTGAAG AGATAGCAGAGATGCTTAAAGTACAGAGTGTCTCAGTTAGTGACATGTTGATGCTCTTGGGACAGAGTCA TCCTACTCCACATGGCTTATTTTTATCAGATGGCCAAGAAGCCATCTATGAGGCTATTCATGATGATCAT TCACCAAATGCAATAGACAGCAATGAAGGCCCATCTAAAGTGACCCAACTCAGGCCAGAATCCCATCACA GTGAGAAAATAGTATTTACTCCTCAGCCCGGCCTCCAGTTAAGATCCAATAAAAGTTTGGAGACAACTAT AGAAGTAAAGTGGAAGAAACTTGGTTTGCAAGTTTCTAGTTTGCCAAGTAATCTAATGACTACAACAATT CTGTCAGACAATTTGAAAGCAACTTTTGAAAAGACAGATTCTTCAGGATTTCCAGATATGCCAGTTCACT CTAGTAGTAAATTAAGTACTACTGCATTTGGTAAGAAAGCATATTCCCTTGTTGGGTCTCATGTACCTTT AAACGTGAGTGAAGAAAATAGTGATTCCAACATATTGGATTCAACTTTAATGTATAGTCAAGAAAGTTTA CCAAGAGATAATATATTATCAATGGAGAATGATAGATTACTCAGAGAGAAGAGGTTTCATGGAATTGCTT TATTGACCAAAGATAATACTTTATTCAAAGACAATGTCTCCTTAATGAAAACAAACAAAACATATAATCA TTCAACAACTAATGAAAAACTACACACTGAGAGCCCAACATCAATTGAGAATAGTACAACAGACTTGCAA GATGCCATATTAAAGGTCAATAGTGAGATTCAAGAAGTAACAGCTTTGATTCATGATGGAACACTTTTAG GCAAAAATTCTACATATTTGAGACTAAACCATATGCTAAATAGAACTACCTCAACAAAAAATAAAGACAT ATTTCATAGAAAAGATGAAGATCCTATTCCACAAGATGAAGAGAATACAATCATGCCATTTTCCAAGATG TTGTTCTTGTCAGAATCTTCAAATTGGTTTAAAAAGACCAATGGAAATAATTCCTTGAACTCTGAGCAAG AACATAGTCCAAAGCAATTAGTATATTTAATGTTTAAAAAATATGTAAAAAATCAAAGTTTCTTGTCAGA GAAAAATAAAGTCACAGTAGAACAGGATGGATTTACAAAGAACATAGGACTTAAAGACATGGCTTTTCCA CATAATATGAGCATATTTCTTACCACTTTGTCTAACGTACATGAAAATGGTAGGCACAATCAAGAAAAAA ATATTCAGGAAGAGATAGAGAAGGAAGCACTAATTGAAGAGAAAGTAGTTTTGCCCCAGGTGCACGAAGC AACTGGCTCTAAGAATTTCTTGAAAGACATATTGATACTAGGCACTAGGCAAAATATAAGTTTATATGAA GTACATGTACCAGTACTTCAAAACATCACATCAATAAACAATTCAACAAATACAGTACAGATTCACATGG AGCATTTCTTTAAAAGAAGGAAGGACAAGGAAACAAATTCAGAAGGCTTGGTAAATAAAACCAGAGAAAT GGTAAAAAACTATCCAAGCCAGAAGAATATTACTACTCAACGTAGTAAACGGGCTTTGGGACAATTCAGA CTGTCAACTCAATGGCTTAAAACCATAAACTGTTCAACACAGTGTATCATTAAACAGATAGACCACAGCA AGGAAATGAAAAAGTTCATTACTAAATCTTCCTTATCAGATTCTTCTGTGATTAAAAGCACCACTCAGAC AAATAGTTCTGACTCACACATTGTAAAAACATCAGCATTTCCACCAATAGATCTCAAAAGGAGTCCATTC CAAAACAAATTTTCTCATGTTCAAGCATCATCCTACATTTATGACTTTAAGACAAAAAGTTCAAGAATTC AAGAAAGCAATAATTTCTTAAAAGAAACCAAAATAAATAACCCTTCTTTAGCCATTCTACCATGGAATAT GTTCATAGATCAAGGAAAATTTACCTCCCCAGGGAAAAGTAACACAAACTCAGTCACATATAAGAAACGT GAGAACATTATTTTCTTGAAACCAACTTTGCCTGAAGAATCTGGCAAAATTGAATTGCTTCCTCAAGTTT CCATTCAAGAGGAAGAAATTTTACCTACAGAAACTAGCCATGGATCTCCTGGACACTTGAATCTCATGAA AGAGGTCTTTCTTCAGAAAATACAGGGGCCTACTAAATGGAATAAAGCAAAGAGGCATGGAGAAAGTATA AAAGGTAAAACAGAGAGCTCTAAAAATACTCGCTCAAAACTGCTAAATCATCATGCTTGGGATTATCATT ATGCTGCACAGATACCAAAAGATATGTGGAAATCCAAAGAGAAGTCACCAGAAATTATATCCATTAAGCA AGAGGACACCATTTTGTCTCTGAGGCCTCATGGAAACAGTCATTCAATAGGGGCAAATGAGAAACAAAAT TGGCCTCAAAGAGAAACCACTTGGGTAAAGCAAGGCCAAACTCAAAGGACATGCTCTCAAATCCCACCAG TGTTGAAACGACATCAAAGGGAACTTAGTGCTTTTCAATCAGAACAAGAAGCAACTGACTATGATGATGC CATCACCATTGAAACAATCGAGGATTTTGACATTTACAGTGAGGACATAAAGCAAGGTCCCCGCAGCTTT CAACAGAAAACAAGGCACTATTTTATTGCAGCTGTGGAACGACTCTGGGACTATGGGATGAGTACATCTC ATGTTCTACGAAATAGGTATCAAAGTGACAATGTACCTCAGTTCAAGAAAGTAGTTTTCCAGGAATTTAC TGATGGCTCCTTTAGTCAGCCCTTATATCGTGGAGAATTAAATGAACACCTGGGGTTGTTGGGCCCATAT ATAAGAGCAGAAGTTGAAGACAACATTATGGTAACTTTCAAAAACCAGGCCTCCCGTCCCTACTCCTTCT ATTCTAGCCTCATTTCTTATAAAGAAGATCAGAGAGGAGAAGAACCTAGAAGAAACTTTGTCAAGCCTAA TGAAACCAAAATTTATTTTTGGAAAGTACAACATCATATGGCACCCACAGAAGATGAGTTTGACTGCAAG GCCTGGGCTTATTTCTCTGATGTTGATCTTGAAAGAGATATGCACTCGGGATTAATTGGACCCCTTCTGA TTTGCCACGCGAACACACTGAATCCTGCTCATGGGAGACAAGTGTCAGTACAGGAATTTGCTCTGCTTTT CACTATCTTTGATGAGACCAAGAGCTGGTACTTCACTGAAAACGTGAAAAGGAACTGCAAGACACCCTGC AATTTCCAGATGGAAGACCCCACTTTGAAAGAGAATTATCGCTTCCATGCAATCAATGGTTATGTAATGG ATACCCTACCAGGCTTAGTAATGGCTCAAGATCAAAGGATTCGATGGTATCTTCTCAGCATGGGCAACAA TGAGAACATCCAATCTATTCATTTCAGTGGACATGTTTTCACTGTACGGAAAAAAGAGGAGTATAAAATG GCAGTGTACAACCTCTACCCAGGTGTTTTTGAGACTCTGGAAATGATACCATCCAGAGCTGGAATATGGC GAGTAGAATGCCTTATTGGCGAGCACTTACAGGCTGGGATGAGCACTCTTTTTCTGGTGTACAGCAAGCA GTGTCAGATTCCTCTTGGAATGGCTTCTGGAAGCATCCGTGATTTCCAGATTACAGCTTCAGGACATTAT GGACAGTGGGCCCCAAACCTGGCAAGACTTCATTATTCCGGATCAATCAATGCCTGGAGTACCAAGGAGC CCTTTTCTTGGATCAAGGTAGATCTGTTGGCACCAATGATTGTTCATGGCATCAAGACTCAGGGTGCTCG TCAGAAATTTTCCAGCCTTTATATCTCTCAATTTATCATCATGTATAGCCTGGATGGGAAGAAGTGGCTG AGTTATCAAGGAAATTCCACTGGAACCTTAATGGTTTTCTTTGGCAATGTGGACTCATCTGGGATTAAGC ATAATAGTTTTAATCCTCCAATTATTGCTCGATATATCCGTTTGCACCCCACTCATTCTAGCATCCGTAG TACTCTTCGCATGGAGTTGATGGGCTGTGATTTAAACAGTTGCAGCATACCATTGGGAATGGAAAGTAAA GTAATATCAGATACACAAATCACTGCCTCATCCTACTTCACCAACATGTTTGCTACTTGGTCTCCTTCAC AAGCTCGACTTCACCTCCAGGGAAGGACTAATGCCTGGCGACCTCAGGTGAATGATCCAAAACAATGGTT GCAAGTGGACTTACAAAAGACAATGAAAGTCACTGGAATAATAACCCAGGGAGTGAAATCTCTCTTTACC AGCATGTTTGTGAAAGAGTTCCTTATTTCCAGCAGTCAAGATGGCCATCACTGGACTCAAATTTTATACA ATGGCAAGGTAAAGGTTTTTCAGGGGAATCAGGACTCATCCACACCTATGATGAATTCTCTAGACCCACC ATTACTCACTCGCTATCTTCGAATTCACCCCCAGATCTGGGAGCACCAAATTGCTCTGAGGCTTGAGATT CTAGGATGTGAGGCCCAGCAGCAATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007977
Insert Size	6960 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007977.2, NP_032003.2
RefSeq Size	7522 bp
RefSeq ORF	6960 bp
Locus ID	14069
UniProt ID	Q06194
Gene Summary	Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.