F8 (NM_007977) Mouse Untagged Clone

CAT#: MC225271

F8 (untagged) - Mouse coagulation factor VIII (F8), transcript variant 1, (10ug)



  "NM_007977" in other vectors (2)

CNY 37,150.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Cf-8; Cf8; FVIII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225271 representing NM_007977
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAATAGCACTCTTCGCTTGCTTCTTTCTGAGCCTTTTCAATTTCTGCTCTAGTGCCATCAGAAGAT
ACTACCTTGGTGCAGTGGAATTGTCCTGGAACTATATTCAGAGTGATCTGCTCAGTGTGCTGCATACAGA
CTCAAGATTTCTTCCTAGAATGTCAACATCTTTTCCATTCAACACCTCCATCATGTATAAAAAGACTGTG
TTTGTAGAGTACAAGGACCAGCTTTTCAACATTGCCAAGCCCAGGCCACCCTGGATGGGTTTGCTAGGTC
CTACCATTTGGACTGAGGTTCATGACACAGTGGTCATTACACTTAAAAACATGGCTTCTCATCCTGTCAG
TCTTCATGCTGTTGGTGTGTCCTACTGGAAAGCTTCTGAGGGAGATGAATATGAAGATCAGACAAGCCAA
ATGGAGAAGGAAGATGATAAAGTTTTCCCTGGTGAAAGTCATACTTATGTTTGGCAAGTCCTGAAAGAGA
ATGGTCCAATGGCCTCTGACCCTCCATGTCTCACTTACTCATATATGTCTCATGTGGATCTGGTGAAAGA
TTTGAATTCAGGCCTCATTGGAGCTCTGCTAGTATGTAAAGAAGGCAGTCTCTCCAAAGAAAGAACACAG
ATGTTGTACCAATTTGTACTGCTTTTTGCTGTATTTGATGAAGGGAAGAGCTGGCACTCAGAAACAAACG
ACTCTTATACACAGTCTATGGATTCTGCATCTGCTAGAGACTGGCCTAAAATGCACACAGTCAATGGCTA
TGTAAACAGGTCTCTTCCAGGTCTGATTGGATGCCATAGGAAATCAGTCTACTGGCACGTGATTGGAATG
GGCACCACTCCTGAAATACACTCAATATTCCTCGAAGGTCACACATTTTTTGTGAGGAACCACCGTCAAG
CTTCATTGGAGATATCACCAATAACTTTCCTTACTGCTCAAACACTCTTGATAGATCTTGGGCAGTTCCT
ACTATTTTGTCATATCTCTTCCCATAAACATGATGGCATGGAAGCTTATGTCAAAGTAGATAGCTGCCCT
GAGGAATCCCAATGGCAAAAGAAAAATAATAATGAGGAAATGGAAGATTATGATGATGATCTTTATTCAG
AAATGGATATGTTCACATTGGATTATGACAGCTCTCCTTTTATCCAAATTCGCTCGGTTGCTAAAAAGTA
CCCTAAAACTTGGATACATTATATTTCTGCTGAGGAGGAAGACTGGGACTATGCACCTTCAGTTCCTACC
TCGGATAATGGAAGTTATAAAAGCCAGTATCTGAGCAATGGTCCTCATCGGATTGGTAGGAAATATAAAA
AAGTCAGATTTATAGCATACACAGATGAAACCTTTAAGACTCGTGAAACTATTCAGCATGAATCAGGACT
CTTGGGACCTTTACTTTATGGAGAAGTTGGAGACACACTGTTGATTATTTTTAAGAATCAAGCAAGCCGA
CCATATAACATTTACCCTCATGGAATCACTGATGTCAGTCCTCTACATGCAAGGAGATTGCCAAGAGGTA
TAAAGCACGTGAAGGATTTGCCAATTCATCCAGGAGAGATATTCAAGTACAAGTGGACAGTTACAGTAGA
AGATGGACCAACTAAATCAGATCCACGGTGCCTGACCCGCTATTATTCAAGTTTCATTAACCCTGAGAGA
GATCTAGCTTCAGGACTGATTGGCCCTCTTCTCATCTGCTACAAAGAATCTGTAGATCAAAGGGGAAACC
AGATGATGTCAGACAAAAGAAATGTCATCCTGTTTTCTATATTTGATGAGAACCAAAGCTGGTACATCAC
AGAGAACATGCAACGCTTCCTCCCCAATGCAGCTAAAACACAGCCCCAGGACCCTGGGTTCCAGGCCTCC
AACATCATGCACAGCATCAATGGCTATGTTTTTGATAGCTTGGAGTTGACAGTTTGTTTGCATGAGGTGG
CATACTGGCACATTCTCAGTGTTGGAGCACAGACAGACTTCTTATCTATCTTCTTCTCTGGATATACTTT
CAAACACAAAATGGTCTATGAAGATACACTTACCCTGTTCCCATTCTCAGGAGAAACTGTCTTTATGTCG
ATGGAAAACCCAGGTCTATGGGTCTTGGGGTGTCATAATTCAGACTTTCGGAAGAGAGGTATGACAGCAT
TGCTGAAAGTTTCTAGTTGTGACAAGAGCACTAGTGATTATTATGAAGAAATATATGAAGATATTCCAAC
ACAGTTGGTGAATGAGAACAATGTCATTGATCCCAGAAGCTTCTTCCAGAATACAAATCATCCTAATACT
AGGAAAAAGAAATTCAAAGATTCCACAATTCCAAAAAATGATATGGAGAAGATTGAGCCTCAGTTTGAAG
AGATAGCAGAGATGCTTAAAGTACAGAGTGTCTCAGTTAGTGACATGTTGATGCTCTTGGGACAGAGTCA
TCCTACTCCACATGGCTTATTTTTATCAGATGGCCAAGAAGCCATCTATGAGGCTATTCATGATGATCAT
TCACCAAATGCAATAGACAGCAATGAAGGCCCATCTAAAGTGACCCAACTCAGGCCAGAATCCCATCACA
GTGAGAAAATAGTATTTACTCCTCAGCCCGGCCTCCAGTTAAGATCCAATAAAAGTTTGGAGACAACTAT
AGAAGTAAAGTGGAAGAAACTTGGTTTGCAAGTTTCTAGTTTGCCAAGTAATCTAATGACTACAACAATT
CTGTCAGACAATTTGAAAGCAACTTTTGAAAAGACAGATTCTTCAGGATTTCCAGATATGCCAGTTCACT
CTAGTAGTAAATTAAGTACTACTGCATTTGGTAAGAAAGCATATTCCCTTGTTGGGTCTCATGTACCTTT
AAACGTGAGTGAAGAAAATAGTGATTCCAACATATTGGATTCAACTTTAATGTATAGTCAAGAAAGTTTA
CCAAGAGATAATATATTATCAATGGAGAATGATAGATTACTCAGAGAGAAGAGGTTTCATGGAATTGCTT
TATTGACCAAAGATAATACTTTATTCAAAGACAATGTCTCCTTAATGAAAACAAACAAAACATATAATCA
TTCAACAACTAATGAAAAACTACACACTGAGAGCCCAACATCAATTGAGAATAGTACAACAGACTTGCAA
GATGCCATATTAAAGGTCAATAGTGAGATTCAAGAAGTAACAGCTTTGATTCATGATGGAACACTTTTAG
GCAAAAATTCTACATATTTGAGACTAAACCATATGCTAAATAGAACTACCTCAACAAAAAATAAAGACAT
ATTTCATAGAAAAGATGAAGATCCTATTCCACAAGATGAAGAGAATACAATCATGCCATTTTCCAAGATG
TTGTTCTTGTCAGAATCTTCAAATTGGTTTAAAAAGACCAATGGAAATAATTCCTTGAACTCTGAGCAAG
AACATAGTCCAAAGCAATTAGTATATTTAATGTTTAAAAAATATGTAAAAAATCAAAGTTTCTTGTCAGA
GAAAAATAAAGTCACAGTAGAACAGGATGGATTTACAAAGAACATAGGACTTAAAGACATGGCTTTTCCA
CATAATATGAGCATATTTCTTACCACTTTGTCTAACGTACATGAAAATGGTAGGCACAATCAAGAAAAAA
ATATTCAGGAAGAGATAGAGAAGGAAGCACTAATTGAAGAGAAAGTAGTTTTGCCCCAGGTGCACGAAGC
AACTGGCTCTAAGAATTTCTTGAAAGACATATTGATACTAGGCACTAGGCAAAATATAAGTTTATATGAA
GTACATGTACCAGTACTTCAAAACATCACATCAATAAACAATTCAACAAATACAGTACAGATTCACATGG
AGCATTTCTTTAAAAGAAGGAAGGACAAGGAAACAAATTCAGAAGGCTTGGTAAATAAAACCAGAGAAAT
GGTAAAAAACTATCCAAGCCAGAAGAATATTACTACTCAACGTAGTAAACGGGCTTTGGGACAATTCAGA
CTGTCAACTCAATGGCTTAAAACCATAAACTGTTCAACACAGTGTATCATTAAACAGATAGACCACAGCA
AGGAAATGAAAAAGTTCATTACTAAATCTTCCTTATCAGATTCTTCTGTGATTAAAAGCACCACTCAGAC
AAATAGTTCTGACTCACACATTGTAAAAACATCAGCATTTCCACCAATAGATCTCAAAAGGAGTCCATTC
CAAAACAAATTTTCTCATGTTCAAGCATCATCCTACATTTATGACTTTAAGACAAAAAGTTCAAGAATTC
AAGAAAGCAATAATTTCTTAAAAGAAACCAAAATAAATAACCCTTCTTTAGCCATTCTACCATGGAATAT
GTTCATAGATCAAGGAAAATTTACCTCCCCAGGGAAAAGTAACACAAACTCAGTCACATATAAGAAACGT
GAGAACATTATTTTCTTGAAACCAACTTTGCCTGAAGAATCTGGCAAAATTGAATTGCTTCCTCAAGTTT
CCATTCAAGAGGAAGAAATTTTACCTACAGAAACTAGCCATGGATCTCCTGGACACTTGAATCTCATGAA
AGAGGTCTTTCTTCAGAAAATACAGGGGCCTACTAAATGGAATAAAGCAAAGAGGCATGGAGAAAGTATA
AAAGGTAAAACAGAGAGCTCTAAAAATACTCGCTCAAAACTGCTAAATCATCATGCTTGGGATTATCATT
ATGCTGCACAGATACCAAAAGATATGTGGAAATCCAAAGAGAAGTCACCAGAAATTATATCCATTAAGCA
AGAGGACACCATTTTGTCTCTGAGGCCTCATGGAAACAGTCATTCAATAGGGGCAAATGAGAAACAAAAT
TGGCCTCAAAGAGAAACCACTTGGGTAAAGCAAGGCCAAACTCAAAGGACATGCTCTCAAATCCCACCAG
TGTTGAAACGACATCAAAGGGAACTTAGTGCTTTTCAATCAGAACAAGAAGCAACTGACTATGATGATGC
CATCACCATTGAAACAATCGAGGATTTTGACATTTACAGTGAGGACATAAAGCAAGGTCCCCGCAGCTTT
CAACAGAAAACAAGGCACTATTTTATTGCAGCTGTGGAACGACTCTGGGACTATGGGATGAGTACATCTC
ATGTTCTACGAAATAGGTATCAAAGTGACAATGTACCTCAGTTCAAGAAAGTAGTTTTCCAGGAATTTAC
TGATGGCTCCTTTAGTCAGCCCTTATATCGTGGAGAATTAAATGAACACCTGGGGTTGTTGGGCCCATAT
ATAAGAGCAGAAGTTGAAGACAACATTATGGTAACTTTCAAAAACCAGGCCTCCCGTCCCTACTCCTTCT
ATTCTAGCCTCATTTCTTATAAAGAAGATCAGAGAGGAGAAGAACCTAGAAGAAACTTTGTCAAGCCTAA
TGAAACCAAAATTTATTTTTGGAAAGTACAACATCATATGGCACCCACAGAAGATGAGTTTGACTGCAAG
GCCTGGGCTTATTTCTCTGATGTTGATCTTGAAAGAGATATGCACTCGGGATTAATTGGACCCCTTCTGA
TTTGCCACGCGAACACACTGAATCCTGCTCATGGGAGACAAGTGTCAGTACAGGAATTTGCTCTGCTTTT
CACTATCTTTGATGAGACCAAGAGCTGGTACTTCACTGAAAACGTGAAAAGGAACTGCAAGACACCCTGC
AATTTCCAGATGGAAGACCCCACTTTGAAAGAGAATTATCGCTTCCATGCAATCAATGGTTATGTAATGG
ATACCCTACCAGGCTTAGTAATGGCTCAAGATCAAAGGATTCGATGGTATCTTCTCAGCATGGGCAACAA
TGAGAACATCCAATCTATTCATTTCAGTGGACATGTTTTCACTGTACGGAAAAAAGAGGAGTATAAAATG
GCAGTGTACAACCTCTACCCAGGTGTTTTTGAGACTCTGGAAATGATACCATCCAGAGCTGGAATATGGC
GAGTAGAATGCCTTATTGGCGAGCACTTACAGGCTGGGATGAGCACTCTTTTTCTGGTGTACAGCAAGCA
GTGTCAGATTCCTCTTGGAATGGCTTCTGGAAGCATCCGTGATTTCCAGATTACAGCTTCAGGACATTAT
GGACAGTGGGCCCCAAACCTGGCAAGACTTCATTATTCCGGATCAATCAATGCCTGGAGTACCAAGGAGC
CCTTTTCTTGGATCAAGGTAGATCTGTTGGCACCAATGATTGTTCATGGCATCAAGACTCAGGGTGCTCG
TCAGAAATTTTCCAGCCTTTATATCTCTCAATTTATCATCATGTATAGCCTGGATGGGAAGAAGTGGCTG
AGTTATCAAGGAAATTCCACTGGAACCTTAATGGTTTTCTTTGGCAATGTGGACTCATCTGGGATTAAGC
ATAATAGTTTTAATCCTCCAATTATTGCTCGATATATCCGTTTGCACCCCACTCATTCTAGCATCCGTAG
TACTCTTCGCATGGAGTTGATGGGCTGTGATTTAAACAGTTGCAGCATACCATTGGGAATGGAAAGTAAA
GTAATATCAGATACACAAATCACTGCCTCATCCTACTTCACCAACATGTTTGCTACTTGGTCTCCTTCAC
AAGCTCGACTTCACCTCCAGGGAAGGACTAATGCCTGGCGACCTCAGGTGAATGATCCAAAACAATGGTT
GCAAGTGGACTTACAAAAGACAATGAAAGTCACTGGAATAATAACCCAGGGAGTGAAATCTCTCTTTACC
AGCATGTTTGTGAAAGAGTTCCTTATTTCCAGCAGTCAAGATGGCCATCACTGGACTCAAATTTTATACA
ATGGCAAGGTAAAGGTTTTTCAGGGGAATCAGGACTCATCCACACCTATGATGAATTCTCTAGACCCACC
ATTACTCACTCGCTATCTTCGAATTCACCCCCAGATCTGGGAGCACCAAATTGCTCTGAGGCTTGAGATT
CTAGGATGTGAGGCCCAGCAGCAATACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_007977
Insert Size 6960 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007977.2, NP_032003.2
RefSeq Size 7522 bp
RefSeq ORF 6960 bp
Locus ID 14069
UniProt ID Q06194
Gene Summary Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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