Atrx (NM_009530) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4833408C14Rik; AI447451; ATR2; DXHXS6677E; HP1-BP38; Hp1bp2; Hp1bp38; MRXS3; Rad54; RAD54L; XH2; Xnp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225333 representing NM_009530 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCTGAGCCCATGAGTGGAAACAAGTTGAGTACATTGGTGCAGAAGCTTCATGACTTCTTAGCAC ACTCATCAGAAGAATCTGAGGAAACGTGTTCTTCTCCACGACTTGTCATGAACCAAAGCACAGATAAAAT CTGTGGTTCTGGACTTAATTCTGATATGATGGAAAATAACAAGGAAGAGGGAGCTAGCACCTCAGAAAAA TCCAGATCTTCAGGTTCATCAAGGTCAAAGAGGAAACCTTCAATTGTGACAAAATATGTAGAATCTGATG ATGAAAAACCCACGGATGAGAATGTAAATGAAAAAGCTGCTACTGAGAATTCAGAAAATGACATTACTAT GCAAAGCTTGCCAAAAGGTACAGTGATTGTACAACCAGAGCCAGTGCTGAATGAAGACAAAGATGATTTT AAAGGGCCTGAATTTAGAAGCAGAAGTAAAATGAAAGCTGACAATCTCAAAAAACGCGGAGAAGATGGAC TTCATGGGATCGTGAGCTGCACAGCTTGTGGACAGCAGGTCAATCATTTTCAAAAAGATTCCATTTATAG ACACCCTTCACTCAAAGTCCTTATCTGTAAGAACTGCTTTAAGTATTATATGAGTGACGATATTAGCCGT GACTCAGATGGAATGGATGAACAATGTAGATGGTGTGCAGAAGGTGGGAACTTGATTTGTTGTGACTTTT GTCATAACGCCTTCTGTAAGAAATGCATCCTGCGCAACCTTGGTCGAAAGGAGTTGTCTACAATAATGGA TGAAAACAATCAGTGGTATTGCTATATTTGTCAACCAGAGCCTCTACTGGACTTGGTGACTGCGTGCAAC AGCGTATTTGAGAATTTAGAACAGTTGTTGCAACAGAATAAGAAGAAGATAAAAGTTGACAGTGAAAAGA CTAGTAAAGTCTGTGACCAAACATCCAAATTTTCTCCAAAGAAGAGTAGTTCTAGTTGTAATGGAGAAGA AAAAAAGTTAGAGGAGTCCTGTTCTGGTTCTGTAAGCTCCACTTACTCTCACTCAGCACTATCTGTGCCC AAAGAGATGATTAAGAAGACAACAAAACTGATTGAGACTACATCCAACATGAACTCCAGTTACATTAAGT TTCTAAAACAGGCAGCAGATAATTCAGAAATGACTTCTGCTATGAAGTTATGTCAACTTAAGTCTTTTAA ATCTGTGTTGGATGATATCAAGAAGGCTCATCTTGCATTAGAAGAAGATTTGAATTCAGAGATCCAAGCT TTGGATGATGTACACAAAGAAAAAAATACCAAAGACCTTAAAAGCACAGATGCTAAGTCTGAAACAAAAT TAGGAAAAGGGGAAAAATCCTATTCTACAGAAAAGAGAGAGTTTTTAAAGTTAGATGCTAGATCATCAGT AAAGGCCATAGATGGTGAAGAGCAGAGAGCACACAAAAGTACTAGTGGGGAACATAAGGGTTCAGGTAGG AAAGATGGCTCTCAGTATGAACCCACTAACACTCCTGAGGATTTAGATATGGATATTGTGTCTGTTCCTT CCTCGGTTCCAGAAGACATTTTTGATAGCCTTGAGAGTGCTATGGAAGTTCAGAGTTCAGCTGATTATCA AGGAGACGGCAACAGTGGGACTGAGCCAGAACTGGAGAGCTCCTCTGTAAAATTAAATGTTTCTTCAAAA GACAGCAGAGGAAATATTAAGTCAAAAGTCACAGCGAAAGTAAGAAAAGAATTATTTGTTAAACTTACCC CAGTTTCCCTTTCTAATTCCCCAATTAAAGGTGTTGACTGTCAGGAAGTGTCACAAGAGAAGAATGGCCG TAAAAGTTCTGGGGTGGCCCGCAGCTCAGAGAAATGTAGACCCAGAGAGGAAATTAGTGATCATGAAAAC AATGTCACAATACTTTTAGAAGACTCCGATCTTCGAAGATCCCCACGTGTAAAAACTACACCGTTGAGGC GACAGACAGAAAGCAACCCTGCAATGTCGAATTCAGATGAAGAAAGTAATGGAACTATGAAGGAGAAACA AAAGATGTCAGGTCCAATAAGAAAAAAGGATAAGAGAAACTCAGCTGACTGTGCTACTGATAACCCTAAA CCTCACAAGGTACCCAAAGCTAAGCAACCAGTGATTGGGGATCAGAATTCAGATTCTGATGAAATGCTAG CAGTCCTCAAAGAGGCAAGTCAGATGGGGCATAGTTCTTCCTCAGACACTGACATTAATGAACCACAGAT GAATCATAAAGGTAAAACAGGGAAAGATGATAATGGAAAAAGGAAACGGAAAAATTCTACTTCCGGGTCA GATTTTGACACTAAAAAAGGAAAATCAACAGAAACCTCTATTATCTCTAAAAAGAAACGCCAGAACTACT CAGAGTCCTCTAACTATGACTCAGAGTTAGAGAGAGAGATAAAAACCATGAGCAGAATTGGGGCTGCCAG AAAAAGTGTTCCAGAGAAAAAAGAAGAGGACTCTTCTGAAGATGAAAAACAGGGCAAAAAAGTAGTGGAT AATGGAGGGCATGAGAGGGCGAAGACAACACAAGAAGGGTCATCTGCTGATGACACTGGTGACACTGAAG GCAGGCAGGGAGGGAGTTGCTCCATAGCAGGAGGTTCCATTGAGAAAGTCAGGAGTGGAGTGGAATTCCG AGAGATGCTTTGCAAACCTGGTGTTTCCTCTGATGGTGCTGAAAAGCCTTCAGTGAAAGAGGAGAACGTG AATTCCCCTGAAGACAAAAGAGTCTCTAAGACTAAAGAAAAGACTAAACATCTGAGAAGCAGACAAAGTA GAAAAGGAAAGGGTGGGTCATCTGATGGCACTGATCGATTTCCAAAGAAAGAGCAGAGCGATGAGTCTTC TGAAGGCGAAAAGAAGCAGAGCAGACAGAGACCTGGAACAAAAGGAAAGAAAGCCCCAGACTTAAAGGGA GAAACACTGAAGCGAGAGCAGGAGTGGGACTCTTCCTCTGATGGGACCGAGAGATTGCCGGAGGAAGAGG AGATTGGTCCTTTTTCTAAAGGCATAAAGCAAAGTAAAACTGACACAGCTGGTGGAGAAAAGAAAGGCAA AAAATGGAAAGATAAATCATGTGAAAAGAAGGAAGAATTATCTGATAGTGTTGATAAATTGCCAGGAAAA GGAGATAGTTGTGACTCTTCCGAAGATAAGAAGACCAGGAACAGAGTGTCTCTGAGAGAGAAGAAACGAT TCAGCTTGCCTGCAAAGAGTCCAGGGAAGAGGCCAGAGTGTTCATCGTCAGATACTGAGAAATCCTTGAA AGGACAGTGTTGTGACTCCACTGAAAAGAGACCGAAAAGAATTGACTTGAGAGAAAGAAGAAACTCCAGT TCAAAGAGAAATACCAAGGAAGTAAAGAGTGCTTCATCCTCTTCTGATGCTGAGGGAAGCTCAGAAGATA ATAAAAAGCAGAAGAAACAAAGAACTTCAGCCAAAAAGAAGACAGGCAATACGAAGGAGAAAAAAAGAAA CTCCTTAAGAGCAACTCCTAAAAGGAAGCAAGTAGACATCACTTCTTCATCTTCTGATATAGGAGATGAT GATCAGAATTCTGCAGGCGAGGAAAGCAGCGATGAACAGAAAATCAAACCCGTGACTGAAAATCTAGTGC TGCCTTCACACACTGGATTTTGTCAGTCTTCAGGAGATGAAGCCTTATCTAAATCAGTGCCTGCCACAGT GGATGATGACGACGACGACAATGATCCTGAAAATAGAATTGCCAAGAAGATGCTTTTAGAAGAAATTAAA GCCAATCTTTCCTCTGATGAGGATGGGTCGTCAGATGATGAACCTGATGGAGGAGGGAAAAAAAGAATTG GGAAACAGAGTGAAGAATCTCCAGCAGATGATGGTGAACTCCGCAGAGAGCAGTTAGCCGTGAATCAAGT CAACTCTGAATCCGACTCTGATTCTGAAGAATCTAAGAAGCCAAGATACAGACATAGGCTTTTGAGGCAT AAACTGACTTTGAGTGATGGAGAATCTGGAGAAGAAAAACCTACTAAGCCTAAAGAGCATAAAGAAGCCA AAGGAAGAAATAGAAGGAAGGTGAGCAGTGAGGATTCAGAAGACACCGACTTTCAGGAGTCAGGAGTTAG CGAAGAAGTCAGTGAATCTGAGGATGAACAGCGGCCTAGAACACGGTCTGCAAAGAAAGCAGAATTGGAA GAAAATCAGCGGAGTTATAAACAGAAGAAGAAAAGACGGCGCATTAAGGTTCAAGAGGATTCCTCCAGTG AAAATAAGAGCCATTCTGAGGAGGACAAGAAGGAAGGTGATGAGGAGGACGAGGAGGACGAGGACGAAGA CGAGGAAGATGAAAATGATGATTCCAAGTCACCTGGAAAAGGCAGAAAGAAAATTCGGAAGATTCTTAAA GATGATAAACTAAGAACAGAAACACAGAATGCCCTTAAAGAAGAGGAAGAAAGGCGAAAACGTATTGCAG AGAGGGAACGTGAAAGGGAGAAATTGAGAGAGGTGATAGAAATTGAGGATGCTTCACCTACTAAGTGTCC AATAACAACCAAATTGGTTTTAGATGAAAATGAAGAGACCAAAGAACCGTTAGTGCAGGTTCATAGAAAC ATGGTTATAAAATTGAAACCCCATCAAGTAGATGGTGTTCAGTTTATGTGGGATTGCTGCTGTGAGTCTG TGGAAAAGACAAAGAAGTCCCCAGGTTCAGGATGTATTCTTGCCCACTGCATGGGCCTTGGTAAGACCCT CCAGGTGGTTAGTTTTCTTCACACAGTTCTTCTGTGTGACAAATTGGATTTCAGTACAGCTTTAGTGGTT TGTCCTCTTAACACTGCTTTGAATTGGATGAATGAATTTGAGAAGTGGCAGGAGGGATTAAATGATAATG AAAAACTTGAGGTATCTGAGTTAGCAACTGTGAAACGTCCTCAGGAGAGAAGTTATATGCTACAGAGATG GCAAGAAGATGGTGGTGTTATGATCATAGGGTATGAAATGTACAGAAATCTCGCTCAAGGAAGAAATGTG AAAAGTCGGAAACTTAAAGACATATTTAATAAAGCTTTAGTTGACCCAGGCCCTGATTTTGTTGTTTGTG ATGAAGGCCATATTCTAAAAAATGAAGCATCTGCTGTTTCAAAAGCTATGAACTCTATAAAATCAAGGAG GAGGATTATTTTAACAGGAACACCACTTCAAAATAACCTAATTGAATATCATTGCATGGTTAACTTTATC AAGGAAAATTTGCTTGGATCCATCAAGGAATTCAGGAATAGATTTATAAATCCAATCCAAAATGGACAGT GTGCAGATTCTACCATGGTAGATGTGAGAGTGATGAAAAAACGTGCTCATATTCTCTATGAGATGTTAGC CGGATGTGTTCAGAGGAAAGATTACACAGCGCTAACAAAGTTCTTACCTCCAAAACATGAGTATGTGTTA GCTGTGAGAATGACTGCTATTCAGTGCAAGCTCTATCAATACTACTTAGATCACTTAACAGGTGTGGGCA ATAGCACTGAAGGTGGAAGAGGAAAGGCAGGTGCAAAGCTTTTTCAAGATTTCCAAATGTTAAGTAGAAT CTGGACTCATCCTTGGTGTTTGCAACTGGACTATATCAGCAAAGAAAATAAGGGTTATTTTGATGAAGAT AGTATGGATGAATTTATAGCTTCAGATTCTGATGAAACCTCTAAGAGTTTAAGCTCAGATGAAAAGAAGA AACCAAAGGGGAAGAAGGGGAAAAAGGACAGCAGCTCAAGCGGAAGTGGCAGTGACAACGATGTTGAAGT CATCAAAGTCTGGAATTCAAGATCTCGAGGAGGTGGAGATGGCAACGTGGATGACACAGGAAATAACCCT TCAGTTTCTCTGAAGCTAGATGAAAGTAAAACTACTTCCACTTCTAACCCAAGTAGCCCAGCTCCAGATT GGTACAAAGATTTTGTTACAGATACTGATGCTGAGGTTTTAGAACATTCTGGAAAAATGGTGCTTCTTTT TGAAATTCTTCGAATGGCAGAAGAAATTGGGGATAAAGTTCTTGTTTTTAGCCAGTCGCTCATATCTCTG GACTTGATTGAAGATTTTCTTGAATTGGCTAGTAGGGAGAAAACAGAAGATAAAGAAAAACCTCTTATTT ATAAAGGTGAAGGAAAGTGGATCCGAAATATTGACTATTACCGCTTGGATGGTTCTACTAATGCACAGTC AAGGAAGAAGTGGGCTGAAGAATTTAATGATGAAACCAATGTGAGAGGGCGATTGTTTATCATTTCCACT AAAGCAGGATCTCTTGGAATTAACCTGGTAGCTGCTAATAGAGTGATTATATTTGATGCTTCTTGGAATC CATCTTATGACATCCAGAGTATATTCAGAGTTTATCGCTTTGGACAAACTAAGCCTGTTTATGTATATAG GTTCTTAGCTCAGGGAACCATGGAAGATAAGATTTATGATCGTCAGGTAACAAAGCAGTCACTGTCTTTC CGCGTTGTGGATCAACAGCAGGTTGAGCGCCATTTTACCATGAATGAGCTTACTGAACTTTATACTTTTG AGCCAGACTTACTAGATGACCCGAATTCAGAAAAGAAGAAGAAAAGGGATACCCCCATGCTGCCAAAGGA TACCATACTTGCAGAACTTCTTCAGATACATAAAGAGCACATTGTGGGCTATCATGAACATGATTCTCTT TTGGATCACAAAGAAGAAGAAGAACTAACTGAAGAAGAAAGAAAAGCAGCTTGGGCTGAATATGAAGCAG AAAAGAAGGGACTGACCATGCGTTTCAACATACCAACTGGGACCAATTTACCCCCTGTCACTTTCACCTC TCAAACTCCTTATATTCCTTTCAATTTGGGAGCCCTGTCCGCAATGAGTAATCAGCAGCTGGAGGACCTT ATTAATCAAGGAAGAGAAAAAGTTGTAGAAGCCACGAACAGTATGACGGCAGTGAGGATTCAGCCTCTTG AAGACATCATTTCAACTGTATGGAAAGAAAACATGAATCTCTCTGAGGCCCAAGTCCAAGCACTAGCATT AAGTAGACAAGCCAGCCAGGAGCTTGACGTGAAACGAAGAGAAGCAATCTACAACGATGTATTGACAAAG CAACAGATGTTGATCAACTGTGTTCAGCGAATACTTATGAATAGACGGCTCCAGCAGCAGTACACTCAGC AGCAACAGCAACAATTGACTTATCAGCAAGCAACACTGAGTCACCTCATGATGCCAAAGCCTCCAAATTT AATCATGACTCCTTCTAACTACCAGCAGATTGATATGAGAGGAATGTATCAGTCAGTGGCTGGTGGTATG CAGCCACCACCCTTACAGCGTGCACCGCCCCCCACAGTGAGAAGCAAAAATCCAGGACCTTCCCCAGGGA AATCAATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009530
Insert Size	7431 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009530.2, NP_033556.2
RefSeq Size	10244 bp
RefSeq ORF	7431 bp
Locus ID	22589
UniProt ID	Q61687
Gene Summary	Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells probably implying recruitment of CBX5 to telomeres. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according (PubMed:20211137) is required for its transcriptional repression in embryonic stem cells. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (By similarity).[UniProtKB/Swiss-Prot Function]

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