Lrrk2 (NM_025730) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921513O20Rik; 9330188B09Rik; AW561911; cI-46; D630001M17Rik; Gm927
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225344 representing NM_025730 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGGCGCCTGTCAGGGCTGCGAAGAGGAAGAGGAGGAGGAGGCTCTGAAGAAGTTGATAGTCA GGCTGAATAATGTCCAGGAAGGCAAGCAGATCGAGACGTTGCTTCAGCTCCTGGAGGACATGCTGGTGTT CACCTACTCGGACCGCGCCTCCAAGTTATTTGAAGATAAAAATTTCCACGTGCCTCTGTTGATTGTCCTG GACTCCTACATGAGAGTTGCCAGTGTACAGCAGGCGGGGTGGTCACTTCTGTGCAAATTAATAGAAGTCT GTCCAGGGACATTGCAAAGCTTAATAGGACCCCAGGATATTGGAAATGATTGGGAAGTCCTTGGTATTCA CCGGCTGATTCTTAAAATGTTAACTGTTCATCACGCCAATGTAAACCTGTCAATAGTTGGACTAAAAGCC TTGGATCTCCTCCTAGATTCAGGTAAACTCACCTTGCTGATACTGGATGAAGAATGTGATATTTTCTTGT TAATTTTTGATGCCATGCACAGATATTCAGCCAATGATGAAGTCCAAAAACTGGGATGCAAAGCTTTACA CGTGCTTTTTGAGAGAGTTTCCGAGGAACAGCTGACTGAGTTTGTGGAGAACAAAGATTACACGATACTG CTGAGTACGTTCGGCAGCTTCAGAAGGGACAAGGAGATTGTGTACCACGTACTTTGCTGCTTGCATTCCC TGGCGGTTACATGCAGCAATGTAGAGGTCCTCATGAGTGGGAATGTCCGGTGCTACAATCTTGTGGTGGA GGCCATGAAAGCCTTCCCCACCAATGAAAACATCCAAGAGGTGAGCTGCTCCTTGTTCCAGAAGCTTACA TTAGGTAACTTTTTCAACATCCTGGTGTTGAACGAAGTGCATGTCTTTGTGGTGAAAGCGGTCCGACAGT ATCCTGAGAACGCAGCCTTACAGATCTCTGCACTCAGCTGTTTAGCACTCCTCACTGAGACTATTTTCTT AAACCAAGACTTGGAGGAAAGAAGTGAGACTCAAGAGCAGAGCGAAGAGGAAGACAGTGAGAAGCTTTTC TGGCTGGAACCCTGCTATAAAGCCCTGGTGCGCCATCGAAAGGACAAACACGTGCAGGAGGCTGCCTGCT GGGCACTAAATAACCTCCTTATGTACCAGAACAGTTTGCATGAGAAGATCGGAGATGAAGATGGCCAGTT CCCTGCGCACAGGGAAGTGATGCTGTCTATGCTGATGCACTCTTCTTCCAAAGATGTCTTCCAAGCAGCT GCACATGCTCTGTCCACTCTCTTGGAACAAAATGTTAATTTCAGGAAAATCCTGCTGGCAAAAGGAGTAT ACCTGAATGTCTTGGAATTGATGCAGAAGCATGCCCATGCGCCTGAGGTGGCAGAGAGTGGCTGCAAGAT GCTGAGTCACCTGTTTGAAGGAAGTAACCCTTCTCTGGATACAATGGCAGCAGTGGTCCCTAAAATACTA ACAGTGATGAAAGCCCACGGAACGTCTCTGTCAGTCCAGCTGGAGGCGCTGCGAGCTATCTTGCATTTCG TTGTGCCAGGACTATTGGAAGAATCCAGGGAGGACTCTCAATGCAGACCAAATGTGCTCAGAAAACAGTG TTTCAGGACTGACATCCACAAGCTGGTTCTAGTCGCTCTGAACAGGTTCATTGGGAATCCTGGGATTCAG AAATGTGGATTGAAAGTAATCTCTTCTCTCGCGCACCTTCCTGATGCCACAGAGACATTGTCCCTGCAAG GAGCAGTTGACTCAGTCCTCCACACCTTACAGATGTATCCAGATGACCAAGAAATTCAGTGTCTGGGCTT ACACCTTATGGGATGCTTGATGACAAAGAAGAATTTCTGCATAGGGACAGGGCACCTCCTGGCAAAAATT CTGGCTTCCACTTTGCAGCGCTTTAAAGATGTTGCTGAGGTGCAGACTACAGGATTACAGACAACCCTGT CAATACTTGAGCTGTCAGTATCTTTCTCCAAGCTGCTAGTGCACTATTCCTTTGATGTGGTGATATTTCA TCAGATGTCTTCCAGTGTTGTAGAACAAAAGGATGAGCAGTTCCTCAATCTATGTTGCAAATGCTTTGCA AAAGTGGCCGTGGATGATGAGCTGAAAAACACCATGCTAGAGAGAGCCTGCGATCAGAATAACAGCATCA TGGTTGAATGTTTGCTCCTCTTGGGAGCTGATGCCAACCAAGTGAAGGGGGCAACTTCTTTAATCTATCA GGTATGTGAGAAAGAGAGCAGTCCTAAATTGGTGGAACTGTTGCTTAATGGTGGTTGTCGTGAACAAGAT GTACGGAAGGCCCTGACCGTAAGCATCCAAAAGGGCGACAGCCAGGTCATCAGCTTGCTCCTCAGGAAAC TTGCCCTGGACCTGGCCAACAACAGCATTTGCCTTGGAGGATTTGGCATAGGAAAAATTGATCCTTCTTG GCTTGGTCCTTTATTTCCAGATAAGTCATCCAATTTAAGGAAGCAAACAAACACAGGATCTGTCCTAGCG AGGAAAGTGCTCCGGTATCAGATGAGAAACACCCTTCAAGAAGGCGTGGCCTCAGGCAGTGACGGCAAGT TTTCTGAAGACGCGCTGGCGAAATTTGGAGAATGGACCTTTATTCCCGACTCTTCTATGGACAGTGTGTT TGGCCAGAGCGATGATCTGGATAGCGAAGGCAGCGAGAGCTCATTTCTCGTGAAGAGGAAGTCCAACTCA ATTAGTGTAGGGGAAGTTTACAGAGATCTAGCTCTGCAGCGCTGCTCACCAAATGCTCAGAGGCATTCCA ATTCGCTGGGTCCTGTTTTTGACCATGAAGACTTACTGAGACGAAAAAGAAAAATACTGTCTTCAGATGA GTCTCTCAGGTCCTCAAGGCTGCCGTCCCATATGAGGCAATCAGATAGCTCTTCTTCCCTGGCTTCTGAG AGAGAACACATCACGTCGTTAGACCTATCTGCCAACGAACTCAAAGATATTGATGCTCTGAGCCAGAAGT GTTGCCTCAGTAGCCACCTGGAACATCTCACCAAACTGGAACTTCACCAGAATTCACTCACGAGCTTCCC ACAGCAGCTGTGTGAGACTCTGAAGTGTTTGATACACTTGGATTTGCACAGTAACAAATTCACCTCATTT CCCTCTTTCGTGTTGAAAATGCCACGTATCACCAACCTAGATGCCTCTCGAAATGACATCGGGCCAACAG TAGTTTTAGACCCTGCGATGAAGTGTCCAAGCCTCAAACAGTTGAATCTGTCCTATAACCAGCTCTCTTC AATCCCAGAGAATCTTGCCCAAGTGGTGGAGAAACTTGAGCAGCTCCTACTGGAAGGAAATAAAATATCC GGGATTTGCTCTCCCCTGAGCCTGAAGGAACTGAAGATTTTAAATCTTAGTAAAAATCACATTCCATCCC TACCTGGAGATTTTCTTGAGGCTTGTTCAAAAGTCGAGAGTTTCAGTGCTCGCATGAATTTTCTTGCTGC AATGCCTGCCTTACCTTCTTCCATAACGAGCTTAAAATTGTCTCAGAACTCTTTCACGTGCATTCCAGAA GCGATTTTCAGTCTTCCGCACTTGCGGTCCTTGGATATGAGCCACAACAACATTGAATGTCTGCCGGGAC CTGCACATTGGAAGTCTCTGAACTTAAGGGAACTCATTTTTAGCAAGAATCAGATCAGCACCTTAGACTT TAGTGAGAACCCACACGTGTGGTCAAGAGTAGAGAAACTGCATCTCTCTCATAATAAACTGAAAGAGATT CCTCCAGAAATTGGCTGCCTTGAAAATCTGACGTCTCTCGACGTCAGTTACAACTTGGAACTGAGGTCCT TCCCAAATGAAATGGGGAAGTTGAGCAAGATATGGGATCTTCCCTTGGACGGACTGCATCTGAATTTTGA CTTTAAGCACGTAGGATGCAAGGCCAAAGACATCATAAGGTTTCTACAACAACGTCTGAAAAAGGCTGTA CCCTACAACCGAATGAAGCTCATGATTGTGGGAAATACGGGGAGCGGTAAGACCACTTTACTGCAACAAC TCATGAAAATGAAGAAACCAGAACTTGGCATGCAGGGTGCCACAGTCGGCATAGACGTGCGAGACTGGTC CATCCAAATACGGGGCAAAAGGAGAAAGGACCTGGTTCTAAACGTGTGGGATTTTGCAGGTCGTGAGGAA TTCTACAGCACTCACCCCCACTTCATGACCCAGAGAGCCCTCTACCTGGCTGTCTATGATCTCAGCAAGG GGCAGGCAGAGGTGGACGCCATGAAGCCCTGGCTCTTCAATATCAAGGCTCGTGCCTCTTCTTCCCCGGT GATTCTGGTGGGCACACATTTGGATGTTTCTGATGAGAAGCAGCGGAAAGCGTGCATAAGCAAAATCACG AAGGAACTCCTAAATAAGCGAGGATTCCCCACCATCCGGGACTACCACTTTGTGAATGCCACCGAGGAGT CAGATGCGCTGGCAAAGCTTCGGAAAACCATCATAAATGAGAGCCTTAATTTCAAGATCCGAGATCAGCC TGTGGTTGGGCAGCTAATTCCAGATTGCTACGTAGAACTGGAGAAAATCATTTTATCAGAGCGGAAAGCT GTGCCGACTGAGTTTCCTGTGATTAACCGGAAACACCTGTTACAGCTCGTGAACGAACATCAGCTGCAGC TGGATGAGAACGAGCTCCCACACGCCGTTCACTTCCTAAATGAGTCGGGAGTTCTTCTGCATTTTCAAGA CCCTGCCCTGCAGCTAAGTGACCTGTACTTTGTGGAACCCAAGTGGCTTTGTAAAGTCATGGCACAGATC TTGACAGTGAAGGTAGACGGCTGTCTGAAACATCCTAAGGGCATCATTTCCCGGAGAGATGTGGAAAAAT TCCTTTCAAAGAAGAAGCGATTCCCGAAGAACTATATGATGCAATACTTTAAACTATTAGAAAAATTTCA GATCGCATTGCCAATAGGGGAAGAATATCTTCTGGTTCCAAGCAGCTTGTCTGACCACAGGCCAGTGATA GAGCTCCCCCACTGTGAGAACTCTGAGATCATCATCCGGCTGTACGAAATGCCGTACTTTCCCATGGGAT TTTGGTCAAGATTGATTAACCGATTACTTGAAATCTCACCCTTCATGCTTTCTGGCAGAGAGAGAGCACT ACGCCCTAACAGGATGTATTGGCGGCAAGGCATCTACTTGAATTGGTCTCCAGAAGCATACTGTCTGGTA GGCTCTGAAGTCTTAGACAATCGACCTGAGAGTTTCTTGAAAATCACAGTTCCGTCTTGTAGAAAAGGTT GTATTCTTCTGGGCCGAGTTGTGGATCATATTGACTCACTCATGGAAGAATGGTTTCCCGGGTTACTGGA GATTGACATTTGTGGGGAAGGAGAAACTCTGTTGAAGAAATGGGCATTGTACAGTTTTAATGATGGTGAA GAACATCAGAAGATCTTGCTTGATGAGTTGATGAAGAAGGCTGAAGAAGGAGACCTGTTAATAAATCCAG ACCAACCAAGGCTCACTATTCCAATATCCCAGATTGCTCCGGACTTGATCTTGGCTGACCTGCCTAGAAA TATCATGTTGAACAATGATGAGTTGGAATTTGAGGAAGCACCAGAGTTTCTCTTAGGCGATGGAAGTTTT GGATCCGTTTATCGAGCTGCCTACGAAGGAGAGGAAGTGGCTGTGAAGATTTTTAATAAGCACACATCTC TTAGGCTGTTAAGACAAGAGTTGGTGGTCCTTTGTCACCTTCACCACCCCAGCCTGATATCCTTGCTGGC GGCTGGTATTCGTCCTCGGATGTTGGTAATGGAGTTGGCCTCCAAAGGTTCCTTGGATCGCCTGCTGCAG CAGGACAAAGCCAGCCTCACCAGAACCCTCCAGCACAGGATCGCGTTGCATGTGGCCGACGGCCTGAGGT ATCTCCACTCAGCCATGATTATTTACCGTGACCTGAAGCCCCACAATGTGCTGCTTTTTACCCTGTATCC CAATGCTGCCATCATTGCGAAGATTGCGGACTACGGGATCGCACAGTACTGCTGCAGGATGGGAATAAAG ACATCAGAGGGCACCCCAGGGTTCCGGGCACCTGAAGTTGCCAGGGGGAATGTCATTTATAACCAACAGG CCGATGTTTATTCTTTTGGCTTACTACTTCACGATATTTGGACAACTGGGAGTAGGATTATGGAGGGTTT GAGGTTCCCAAATGAGTTTGATGAGTTAGCCATACAAGGGAAGTTGCCAGATCCAGTTAAAGAATATGGC TGTGCCCCATGGCCTATGGTTGAGAAGTTAATTACAAAGTGTTTGAAAGAAAATCCTCAAGAAAGACCCA CTTCTGCCCAGGTCTTTGACATTTTGAATTCGGCTGAATTAATTTGCCTCATGCGACACATTTTAATACC TAAGAACATCATTGTTGAATGCATGGTTGCCACGAATCTCAATAGCAAGAGTGCGACTCTCTGGTTGGGA TGTGGGAACACAGAAAAAGGACAGCTTTCCTTATTTGACTTAAACACGGAAAGATACAGCTATGAGGAAG TTGCTGATAGTAGAATACTGTGCTTGGCTTTGGTGCATCTCGCTGCTGAGAAAGAGAGCTGGGTTGTGTG CGGGACACAGTCTGGGGCTCTCCTGGTCATCAATGTTGAAGAGGAGACAAAGAGACACACCCTGGAAAAG ATGACTGATTCTGTCACTTGTTTGCATTGCAATTCCCTTGCCAAGCAGAGCAAGCAAAGTAACTTTCTTT TGGTGGGAACTGCTGATGGTAACTTAATGATATTTGAAGATAAAGCCGTTAAGTGTAAAGGAGCTGCCCC CTTGAAGACACTACACATAGGCGATGTCAGTACGCCCCTGATGTGCCTGAGCGAGTCCCTGAATTCATCT GAAAGACACATCACATGGGGAGGGTGTGGCACAAAGGTCTTCTCCTTTTCCAATGATTTCACCATTCAGA AACTCATCGAGACAAAAACCAACCAGCTGTTTTCTTACGCAGCTTTCAGCGATTCTAACATCATAGCGCT GGCAGTAGACACAGCCCTGTATATTGCCAAGAAAAACAGCCCTGTCGTAGAGGTGTGGGACAAGAAAACA GAAAAGCTCTGTGAATTAATAGACTGTGTGCACTTCTTAAAGGAGGTGATGGTAAAACTAAACAAGGAAT CGAAACATCAGCTGTCCTACTCTGGGAGGGTGAAGGCCCTCTGCCTGCAGAAGAACACGGCTCTCTGGAT CGGAACTGGAGGAGGCCACATCTTACTCCTGGATCTTTCTACTCGGCGAGTTATCCGCACCATTCACAAT TTCTGTGATTCTGTGAGAGCCATGGCCACAGCACAATTAGGAAGCCTTAAGAATGTCATGCTGGTTTTGG GGTACAAGCGGAAGAGTACAGAGGGTATCCAAGAACAAAAAGAGATACAATCTTGTTTGTCTATTTGGGA CCTCAATCTTCCACACGAGGTGCAAAATTTAGAAAAACACATTGAAGTAAGAACAGAATTAGCTGATAAA ATGAGGAAAACATCTGTTGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_025730
Insert Size	7584 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_025730.3, NP_080006.3
RefSeq Size	8231 bp
RefSeq ORF	7584 bp
Locus ID	66725
UniProt ID	Q5S006
Gene Summary	Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Phosphorylates PRDX3. Has GTPase activity (By similarity). Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization (PubMed:25201882).[UniProtKB/Swiss-Prot Function]

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