Fbn1 (NM_007993) Mouse Untagged Clone

CAT#: MC225423

Fbn1 (untagged) - Mouse fibrillin 1 (Fbn1), (10ug)



  "NM_007993" in other vectors (2)

CNY 46,080.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI536462; B430209H23; Fib-; Fib-1; Tsk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225423 representing NM_007993
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCGAGGAGGGCTGCTGGAGGTCGCGCTGGCGTTCGCCCTGCTCCTCGAGTCCTACACGAGCCATG
GGGCGGACGCCAATTTGGAGGCTGGGAGCCTGAAGGAGACCAGAGCCAATCGGGCCAAGAGAAGAGGCGG
CGGAGGACACGATGCGCTGAAAGGACCCAATGTCTGTGGATCACGTTATAATGCATACTGTTGTCCTGGA
TGGAAAACCTTACCTGGTGGAAATCAGTGTATTGTTCCCATTTGCCGGCATTCCTGTGGGGATGGATTCT
GCTCGAGGCCAAATATGTGCACTTGCCCGTCTGGTCAGATATCTCCTTCCTGTGGCTCCAGATCCATCCA
ACACTGCAGCATCCGCTGTATGAATGGGGGCAGCTGCAGCGATGACCACTGTCTGTGCCAGAAAGGGTAC
ATCGGCACTCACTGTGGACAGCCTGTCTGTGAAAGTGGCTGTCTCAACGGAGGGAGGTGTGTGGCCCCAA
ATCGGTGTGCTTGCACGTACGGCTTTACTGGACCCCAGTGTGAAAGAGATTACAGAACAGGCCCATGTTT
TACTGTGGTAAGCAACCAGATGTGCCAGGGACAGCTCAGCGGGATTGTCTGCACCAAAACACTTTGCTGT
GCCACCGTGGGCCGAGCCTGGGGCCACCCCTGTGAGATGTGTCCTGCCCAGCCTCACCCCTGCCGCCGCG
GCTTCATTCCCAACATCCGCACTGGAGCTTGTCAAGATGTGGATGAATGTCAGGCCATCCCAGGGATGTG
TCAAGGAGGAAATTGCATTAATACCGTTGGATCTTTTGAGTGCAAATGCCCTGCTGGACACAAATTTAAT
GAAGTGTCACAAAAATGTGAAGATATTGACGAGTGCAGCACCATTCCTGGAGTCTGCGATGGCGGGGAAT
GTACAAACACTGTCAGCAGCTACTTCTGCAAATGTCCCCCTGGTTTTTACACCTCTCCTGATGGCACCAG
ATGCGTAGATGTTCGCCCTGGTTACTGCTACACAGCTCTGGCAAACGGGCGCTGCTCTAACCAGCTGCCA
CAGTCCATAACCAAAATGCAGTGCTGTTGCGATCTTGGCCGGTGCTGGTCTCCAGGGGTTACTGTTGCTC
CCGAGATGTGTCCCATCAGGTCAACTGAGGATTTCAACAAGCTGTGCTCTGTCCCTCTGGTAATTCCCGG
GAGACCAGAATATCCTCCCCCACCCATTGGCCCCCTTCCTCCAGTTCAGCCCGTTCCTCCTGGCTATCCT
CCTGGGCCTGTGATTCCAGCCCCTCGGCCACCGCCAGAATATCCATATCCATCTCCGTCTCGGGAACCAC
CAAGGGTGCTGCCTTTCAACGTTACTGACTACTGTCAACTGGTCCGCTATCTCTGTCAAAATGGGCGCTG
CATTCCAACTCCCGGTAGCTACCGCTGCGAGTGCAACAAGGGCTTCCAGCTGGATATCCGTGGCGAATGC
ATCGACGTGGATGAGTGTGAGAAGAACCCATGCACTGGTGGCGAGTGCATCAACAACCAGGGCTCCTACA
CCTGTCACTGCAGAGCTGGCTACCAGAGCACACTCACCAGAACTGAGTGCAGAGACATAGATGAGTGTCT
TCAGAATGGCCGGATCTGCAACAATGGTCGCTGTATCAACACAGACGGCAGCTTCCACTGCGTATGCAAT
GCGGGCTTTCATGTCACGCGGGACGGAAAGAACTGTGAAGATATGGATGAGTGCAGCATCCGAAACATGT
GCCTAAACGGAATGTGTATTAATGAAGATGGCAGTTTCAAGTGTATTTGCAAACCTGGGTTCCAACTGGC
ATCAGATGGCCGCTACTGCAAAGATATCAATGAGTGTGAGACACCTGGGATCTGCATGAACGGACGCTGT
GTGAACACGGATGGCTCCTACAGATGCGAATGCTTCCCCGGATTGGCTGTGGGTCTAGACGGACGTGTGT
GTGTTGACACACACATGCGGAGCACATGCTATGGAGGATACAGGAGAGGCCAGTGCGTGAAGCCGTTGTT
TGGTGCTGTTACCAAATCGGAATGCTGTTGTGCCAGCACTGAGTATGCCTTTGGGGAACCCTGCCAGCCG
TGTCCTGCACAGAATTCAGCGGAATATCAGGCACTCTGCAGCAGTGGACCGGGAATGACATCAGCAGGCA
CTGATATAAACGAATGTGCATTAGATCCTGATATTTGCCCAAATGGAATTTGTGAAAATCTCCGTGGGAC
CTACAAATGTATATGCAACTCGGGATATGAAGTAGACATAACTGGGAAAAACTGTGTCGATATTAATGAG
TGTGTGCTGAACAGTCTACTTTGTGACAATGGACAATGTCGAAACACACCTGGAAGTTTTGTCTGCACCT
GCCCCAAAGGATTTGTGTACAAACCTGACCTAAAAACCTGTGAAGACATTGATGAATGTGAATCGAGTCC
TTGCATTAATGGAGTCTGCAAGAACAGCCCTGGCTCCTTCATTTGTGAATGTTCTCCTGAAAGTACTCTG
GACCCAACAAAAACCATCTGCATAGAAACCATCAAGGGCACTTGCTGGCAGACTGTCATCGACGGGCGCT
GTGAGATCAACATCAACGGAGCCACCTTGAAGTCCGAGTGCTGCTCCTCCCTTGGTGCTGCGTGGGGGAG
CCCGTGCACCATCTGTCAACTTGATCCCATTTGTGGTAAAGGGTTCTCAAGAATTAAAGGCACGCAATGT
GAAGATATCAATGAGTGTGAAGTGTTCCCGGGAGTATGCAAGAACGGCCTGTGTGTCAACTCCAGGGGTT
CATTCAAGTGCGAGTGTCCCAATGGAATGACTTTGGATGCTACAGGAAGAATCTGTCTTGACATCCGCCT
GGAGACCTGCTTCCTCAAGTATGACGATGAAGAGTGCACCTTGCCCATCGCTGGCCGCCACCGAATGGAT
GCCTGCTGCTGCTCTGTTGGGGCAGCCTGGGGAACGGAAGAGTGTGAGGAGTGTCCATTGAGAAACAGCC
GGGAGTATGAGGAACTCTGTCCCCGAGGACCTGGGTTTGCCACAAAAGACATTACAAATGGGAAACCTTT
CTTCAAAGATATCAATGAGTGCAAGATGATACCCAGCCTCTGTACCCACGGCAAGTGCAGGAACACCATT
GGCAGCTTCAAGTGTAGGTGTGACAGTGGCTTTGCTCTGGATTCTGAAGAGAGGAACTGTACAGACATTG
ATGAGTGCCGCATATCTCCTGACCTCTGTGGCCGAGGCCAGTGTGTGAACACCCCGGGGGACTTTGAATG
CAAGTGTGATGAAGGCTATGAAAGTGGCTTCATGATGATGAAGAACTGCATGGATATTGATGAATGTCAG
AGAGATCCTCTCCTGTGTCGAGGAGGCATTTGCCACAACACAGAGGGAAGCTATCGCTGCGAATGTCCTC
CTGGTCACCAATTGTCCCCAAACATCTCTGCATGCATTGACATCAACGAGTGTGAGCTGAGTGCGAATCT
CTGTCCCCATGGGCGTTGTGTGAACCTCATAGGGAAGTACCAGTGTGCCTGCAACCCTGGCTACCACCCC
ACTCATGACAGGCTCTTCTGTGTCGATATTGATGAATGCAGCATAATGAACGGTGGTTGTGAGACCTTCT
GCACAAACTCTGACGGGAGCTATGAATGTAGCTGTCAGCCAGGCTTCGCGCTAATGCCAGACCAGCGATC
GTGCACAGACATTGATGAGTGTGAAGACAACCCCAATATCTGTGATGGTGGCCAGTGCACAAACATACCT
GGGGAGTACAGGTGCCTGTGCTATGATGGGTTCATGGCATCTGAAGACATGAAGACTTGTGTGGATGTCA
ATGAGTGTGACCTGAATCCAAACATCTGCCTTAGTGGGACCTGTGAAAATACTAAAGGCTCGTTCATCTG
CCACTGTGATATGGGATATTCAGGGAAGAAAGGAAAAACGGGCTGTACAGATATCAATGAATGTGAGATC
GGAGCACACAACTGTGGCAGACATGCTGTATGCACAAATACAGCCGGGAGCTTCAAGTGCAGCTGCAGTC
CCGGCTGGATTGGAGACGGCATTAAGTGCACAGATCTGGATGAATGCTCTAATGGAACCCACATGTGCAG
CCAACACGCGGACTGCAAGAACACCATGGGGTCATATCGCTGTCTCTGTAAGGATGGCTATACAGGGGAT
GGCTTCACCTGTACAGACCTCGACGAGTGCTCCGAGAACCTGAACCTCTGTGGCAATGGCCAGTGCCTCA
ACGCCCCTGGCGGGTACCGCTGTGAATGCGACATGGGCTTCGTGCCCAGTGCTGACGGGAAGGCCTGTGA
AGATATCGATGAGTGCTCCCTTCCAAACATCTGTGTCTTTGGAACTTGCCACAACCTCCCGGGCCTCTTC
CGTTGCGAGTGTGAGATTGGCTATGAACTGGACCGAAGTGGTGGAAACTGCACAGATGTTAATGAGTGTC
TGGATCCCACCACCTGCATCAGTGGAAACTGTGTCAACACTCCCGGTAGTTACACATGCGATTGTCCTCC
GGATTTTGAGCTGAATCCAACTCGTGTCGGCTGTGTCGATACTCGCTCTGGAAACTGCTATCTGGATATC
CGACCCCGGGGAGACAATGGAGATACAGCCTGCAGCAATGAAATTGGAGTTGGTGTCTCTAAGGCTTCCT
GCTGTTGTTCACTGGGTAAAGCTTGGGGAACCCCATGTGAGCTGTGTCCTTCTGTGAACACATCTGAGTA
TAAAATTCTTTGCCCTGGAGGAGAAGGTTTTCGTCCAAATCCCATCACCGTTATATTGGAAGACATCGAT
GAGTGCCAGGAGCTTCCAGGGCTGTGCCAAGGGGGGAAGTGCATCAATACCTTTGGCAGCTTCCAGTGTC
GCTGTCCAACTGGTTACTACCTGAATGAAGACACTCGAGTGTGTGATGATGTGAACGAATGTGAGACTCC
TGGAATCTGTGGTCCGGGGACCTGTTACAACACCGTTGGCAACTATACCTGCATTTGTCCTCCAGACTAC
ATGCAAGTGAACGGGGGAAATAATTGCATGGACATGAGAAGAAGTCTATGCTACAGAAACTATTACGCTG
ACAACCAGACCTGCGATGGAGAACTCCTGTTCAACATGACCAAGAAGATGTGCTGTTGCTCCTACAACAT
CGGCAGAGCCTGGAACAAACCCTGTGAACAGTGCCCCATCCCAAGCACAGATGAGTTTGCTACCCTCTGT
GGGAGCCAGAGGCCCGGCTTCGTGATTGACATTTATACGGGTTTACCCGTGGATATTGATGAATGCCGGG
AGATCCCTGGGGTCTGTGAAAATGGAGTGTGCATCAACATGGTTGGCAGCTTCCGGTGTGAGTGTCCCGT
GGGATTCTTCTATAACGACAAGTTACTGGTTTGTGAAGATATCGACGAGTGTCAGAATGGCCCTGTGTGC
CAGCGAAATGCGGAATGCATCAACACTGCAGGCAGCTACCGCTGTGACTGTAAGCCCGGCTACCGCCTTA
CCTCCACAGGTCAATGCAACGATCGAAACGAGTGCCAAGAAATCCCGAACATATGCAGTCATGGCCAGTG
CATCGACACCGTGGGAAGCTTCTACTGCCTTTGTCACACTGGCTTCAAAACAAATGTGGATCAGACCATG
TGCTTAGACATAAATGAGTGTGAGAGAGACGCCTGTGGGAACGGGACTTGCAGAAACACGATTGGCTCCT
TCAACTGTCGCTGTAACCATGGCTTCATACTGTCTCACAACAATGACTGCATAGATGTTGATGAGTGTGC
AACTGGAAACGGGAACCTTTGCAGAAATGGCCAGTGTGTCAATACCGTGGGCTCCTTTCAGTGCAGGTGC
AATGAAGGCTATGAGGTGGCTCCGGACGGCAGGACCTGTGTGGATATCAACGAGTGTGTTCTGGATCCTG
GGAAATGTGCACCTGGAACCTGTCAGAACCTGGATGGCTCCTACAGATGCATTTGCCCGCCTGGGTATAG
TCTACAGAATGACAAGTGTGAAGATATTGATGAGTGTGTTGAAGAGCCAGAAATCTGTGCCTTGGGGACC
TGCAGCAACACTGAGGGTAGCTTCAAATGTCTGTGTCCAGAGGGGTTCTCCCTGTCCTCCACTGGAAGAA
GGTGCCAAGATTTGCGAATGAGCTACTGCTATGCGAAGTTTGAAGGTGGGAAGTGTTCATCACCCAAATC
CAGAAACCATTCCAAGCAGGAGTGCTGCTGTGCTTTGAAGGGAGAAGGCTGGGGAGATCCTTGTGAGTTG
TGCCCCACTGAGCCAGATGAGGCTTTCCGCCAGATCTGCCCCTTTGGAAGTGGGATCATTGTGGGCCCTG
ATGACTCAGCAGTTGATATGGACGAATGCAAAGAACCTGATGTCTGTAGACATGGGCAGTGCATTAACAC
AGACGGCTCCTATCGATGCGAGTGTCCTTTTGGTTATATTCTGGAAGGGAATGAGTGTGTGGATACCGAT
GAATGCTCTGTGGGCAATCCTTGTGGAAATGGGACCTGCAAGAATGTGATTGGAGGTTTTGAATGTACCT
GTGAGGAGGGGTTCGAGCCTGGCCCAATGATGACTTGTGAAGATATAAATGAATGTGCCCAGAATCCTCT
GCTCTGCGCCTTCCGCTGTGTAAATACCTACGGGTCCTATGAATGCAAATGCCCTGTTGGATACGTTCTC
CGAGAAGACAGGAGGATGTGTAAAGATGAGGATGAGTGTGCAGAGGGAAAACACGACTGTACTGAGAAGC
AAATGGAGTGTAAGAACCTCATTGGTACCTACATGTGCATCTGCGGCCCTGGGTACCAGCGCAGACCCGA
TGGAGAGGGCTGCATAGATGAGAATGAGTGTCAGACCAAGCCCGGGATCTGTGAGAATGGGCGTTGCCTC
AACACCCTGGGTAGCTACACTTGTGAGTGTAACGATGGCTTCACAGCCAGCCCCACTCAGGATGAGTGCT
TGGACAACCGGGAAGGGTACTGCTTTTCGGAGGTCTTGCAAAACATGTGCCAGATTGGCTCAAGCAACAG
GAACCCCGTCACCAAGTCCGAGTGCTGCTGTGATGGAGGGAGAGGCTGGGGACCCCACTGTGAGATCTGC
CCTTTCGAGGGCACAGTGGCTTACAAGAAGCTCTGTCCCCACGGCCGAGGATTCATGACCAACGGAGCAG
ATATTGATGAGTGCAAGGTTATTCATGATGTTTGCCGAAATGGGGAGTGTGTCAACGACAGAGGGTCCTA
TCACTGCATCTGTAAAACTGGCTACACTCCGGATATAACAGGGACCGCCTGTGTAGATCTGAATGAATGC
AACCAGGCTCCCAAACCCTGCAATTTTATATGCAAAAACACAGAAGGGAGTTACCAGTGTTCCTGCCCGA
AGGGCTACATTCTGCAAGAGGATGGAAGGAGCTGCAAAGATCTTGACGAGTGTGCAACCAAGCAGCATAA
CTGTCAGTTCCTGTGTGTTAACACCATCGGTGGCTTCACATGCAAATGCCCTCCTGGGTTTACCCAGCAT
CACACTGCCTGCATTGATAACAATGAGTGCACGTCTGATATCAACCTGTGTGGGTCCAAGGGTGTTTGCC
AGAACACTCCAGGAAGCTTCACCTGTGAATGCCAACGGGGGTTCTCACTCGATCAGAGTGGTGCCAGCTG
TGAAGATGTGGACGAGTGTGAGGGTAACCACCGCTGTCAACATGGCTGCCAGAACATCATCGGAGGCTAT
AGGTGTAGCTGCCCCCAGGGCTACCTCCAGCACTACCAATGGAACCAGTGTGTAGATGAAAACGAGTGCC
TGAGTGCACATGTCTGTGGAGGAGCCTCCTGCCACAACACCCTGGGGAGTTACAAGTGCATGTGTCCCAC
CGGCTTCCAGTACGAACAGTTCAGTGGAGGCTGCCAAGACATCAATGAGTGTGGCTCATCCCAGGCCCCC
TGCAGTTACGGTTGCTCTAATACTGAGGGTGGCTACCTGTGTGGCTGTCCACCAGGATACTTCCGGATAG
GCCAAGGGCATTGTGTTTCTGGAATGGGCATGGGCCGAGGCGGCCCAGAGCCACCTGCCAGCAGCGAGAT
GGACGACAACTCACTGTCCCCAGAGGCCTGCTATGAGTGTAAGATCAACGGCTACCCAAAACGAGGCCGG
AAACGGAGAAGCACGAACGAAACGGATGCCTCCGACATCCAGGACGGGTCTGAGATGGAAGCCAACGTGA
GCCTCGCCAGCTGGGATGTGGAGAAGCCGGCTAGCTTTGCTTTCAATATTTCCCATGTCAATAACAAGGT
CCGAATCCTAGAGCTCCTGCCGGCCCTCACAACTCTGATGAACCACAACAGATACTTGATTGAATCTGGA
AATGAAGATGGCTTCTTTAAAATCAACCAGAAAGAAGGGGTCAGCTACCTCCACTTCACGAAGAAGAAGC
CGGTGGCTGGGACCTACTCCTTACAAATCAGCAGCACCCCACTTTATAAAAAGAAAGAACTTAACCAGTT
AGAAGACAGATATGACAAAGACTACCTCAGTGGTGAACTGGGCGATAACCTGAAGATGAAAATTCAGATC
TTGCTGCATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_007993
Insert Size 8622 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007993.2, NP_032019.2
RefSeq Size 9900 bp
RefSeq ORF 8622 bp
Locus ID 14118
UniProt ID Q61554
Gene Summary This gene encodes a member of the fibrillin family of proteins. The encoded preproprotein is proteolytically processed to generate two proteins including the extracellular matrix component fibrillin-1 and the protein hormone asprosin. Fibrillin-1 is an extracellular matrix glycoprotein that serves as a structural component of calcium-binding microfibrils. Asprosin, secreted by white adipose tissue, has been shown to regulate glucose homeostasis. Homozygous knockout mice for this gene exhibit impaired aortic development and early postnatal death, which was attributed to a deficiency in the fibrillin-1 protein. Mice with a hypomorphic allele of this gene exhibit impaired glucose homeostasis, likely due to a reduction in serum asprosin levels. [provided by RefSeq, Apr 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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