Trio (NM_001081302) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6720464I07Rik; AA408740; Solo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225460 representing NM_001081302 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCAGCAGCGGCGGTGCCACCGCCCCGGCCGCGTCCTCCGGCCCCGCGGCAGCGGCCAGCGCGG CGGGCTCCGGCTGCGGGGGCGGCGCCGGCGAGGGCGCCGAGGAGGCCGCCAAGGACCTAGCCGACATCGC GGCCTTCTTCCGATCCGGGTTTCGGAAAAATGATGAAATGAAAGCTATGGATGTTTTGCCAATTCTGAAG GAAAAAGTCGCCTATCTTTCAGGTGGTAGAGATAAGCGTGGAGGTCCCATTTTAACATTTCCAGCCCGCA GCAATCATGACAGAATACGGCAGGAGGACCTCAGGAGGCTCATTTCCTATTTAGCCTGCATTCCCAGTGA GGAAGTCTGCAAGCGAGGGTTCACAGTGATTGTGGACATGCGCGGCTCCAAGTGGGACTCCATCAAGCCG CTGCTGAAGATCCTGCAGGAGTCCTTTCCGTGCTGCATCCACATAGCCCTGATAATCAAGCCCGACAACT TCTGGCAGAAACAGAGGACGAATTTTGGCAGCTCCAAATTTGAATTTGAGACAAATATGGTCTCCTTAGA AGGCCTTACCAAAGTAGTTGATCCTTCCCAGCTAACTCCCGAGTTTGACGGCTGCCTGGAATACAACCAT GAGGAGTGGATAGAAATCCGAGTAGCTTTCGAGGAGTACATTAGCAATGCAGCCCATATGCTGTCACGGC TAGAGGAACTTCAGGATGTTCTCGCCAAAAAGGAGTTGCCTCAGGACCTAGAGGGGGCTCGGAACATGAT CGACGAGCACTCCCAGTTGAAGAAGAAGGTGATCAAGGCCCCCATTGAGGACTTGGACTTAGAAGGGCAG AAGCTGCTTCAGCGGATACAGAGCAGCGACAGTTTCCCCAAAAAGAACTCAGGCTCGGGCAATGCAGACC TGCAGAACCTCCTGCCCAAGGTGTCCACCATGCTAGACAGGCTGCACTCCACCCGGCAGCACCTCCACCA GATGTGGCACGTGAGGAAACTGAAGCTGGACCAGTGCTTCCAGCTGCGGCTGTTCGAACAGGATGCTGAG AAGATGTTTGACTGGATCACTCACAACAAAGGCCTGTTTCTGAACAGCTACACGGAGATCGGGACCAGCC ACCCGCATGCCATGGAACTTCAGACGCAGCACAATCATTTTGCCATGAACTGTATGAACGTATATGTAAA TATAAACCGCATAATGTCAGTGGCCAATCGCCTGGTGGAGTCGGGCCACTACGCCTCTCAGCAGATTAAG CAGATTGCAAATCAGCTGGAACAAGAGTGGAAGGCGTTTGCAGCAGCCTTGGACGAGCGCAGCACCTTGT TGGACATGTCCTCCATCTTTCACCAGAAGGCTGAGAAGTACATGAGCAACGTGGACTCGTGGTGTAAGGC CTGTGGTGAAGTAGACCTTCCCTCGGAGCTGCAGGACCTGGAAGACGCCATTCATCATCACCAGGGCATC TACGAGCACATCACCCTCGCCTACTCAGAGGTGAGCCAGGATGGGAAGTCCCTCCTTGATAAGCTCCAGC GCCCCCTGACCCCCGGCAGCTCCGACTCCCTCACCGCCTCCGCCAACTACTCCAAGGCTGTGCACCACGT CTTGGATGTCATCCACGAGGTCCTGCACCACCAGCGCCAGCTGGAGAACATCTGGCAGCACCGCAAGGTT CGGCTCCACCAGAGGCTGCAGCTCTGCGTCTTCCAGCAGGACGTTCAGCAGGTGCTGGACTGGATAGAGA ACCACGGAGAAGCGTTTCTGAGCAAACACACGGGCGTGGGCAAATCCCTTCACCGGGCCAGAGCTTTGCA GAAACGCCACGAGGACTTCGAGGAAGTCGCACAGAACACGTACACAAACGCAGATAAACTGCTAGAAGCA GCAGAGCAGCTGGCTCAGACGGGGGAATGTGACCCCGAAGAGATTTATCAGGCTGCCCATCAGCTTGAAG ACCGGATACAAGATTTTGTTCGGCGAGTTGAACAACGAAAGATCCTGCTGGACATGTCTGTGTCATTTCA CACGCACGTAAAAGAGCTCTGGACATGGCTGGAGGAGTTACAGAAGGAACTGCTGGACGACGTGTACGCC GAGTCGGTGGAGGCCGTGCAGGACCTCATCAAGCGATTCGGCCAGCAGCAGCAGACCACCCTGCAGGTGA CTGTCAACGTGATCAAGGAAGGAGAAGACCTCATCCAGCAGCTGAGGGACTCCGCCATCTCCAGTAACAA AACTCCGCACAACAGTTCCATCAACCACATCGAGACGGTGCTACAGCAGCTGGACGAGGCTCAGTCCCAG ATGGAGGAGCTCTTCCAGGAGCGCAAGATCAAGCTGGAGCTCTTCCTGCAGCTGCGCATCTTCGAGCGGG ATGCCATCGATATCATCTCGGACCTTGAGTCGTGGAATGATGAGCTTTCCCAGCAAATGAATGACTTCGA TACGGAGGACCTGACGATCGCAGAGCAGCGCCTACAGCACCATGCAGACAAAGCCCTGACCATGAATAAC TTGACTTTCGATGTCATCCACCAAGGACAGGATCTTCTGCAGTATGTCAATGAGGTCCAGGCCTCTGGTG TGGAGCTGCTTTGTGACAGAGATGTGGACATGGCCACTCGGGTTCAGGACCTCCTGGAGTTTCTTCATGA GAAGCAGCAGGAGTTGGATTTGGCAGCTGAGCAGCATCGGAAACACTTGGAGCAGTGTGTGCAGTTGCGC CACCTGCAGGCGGAAGTGAAGCAGGTGCTGGGCTGGATCCGAAATGGAGAGTCCATGCTAAATGCTGGGC TCATCACGGCCAGCTCGCTGCAGGAAGCCGAGCAGCTGCAGCGAGAACACGAGCAGTTCCAGCATGCGAT TGAGAAAACGCACCAGAGCGCGCTGCAGGTACAGCAGAAGGCGGAGGCCATGCTGCAGGCCAACCACTAT GACATGGACATGATCCGGGACTGTGCAGAGAAGGTCGCCTCGCACTGGCAGCAGCTCATGCTGAAGATGG AAGACCGCCTCAAGCTTGTCAATGCCTCTGTCGCCTTCTACAAGACTTCTGAGCAGGTCTGCAGTGTCCT GGAAAGTCTGGAACAGGAGTACAAGAGAGAAGAGGACTGGTGTGGCGGGGCCGACAAGCTGGGCCCCAAT TCTGAGACTGACCACGTCACCCCCATGATTAGCAAGCACCTCGAACAAAAGGAAGCCTTCCTGAAGGCTT GCACACTGGCCAGGAGGAACGCAGACGTCTTCCTAAAGTACCTGCACAGGAACAGCGTGAGCATGCCGGG GATGGTGACGCACATCAAAGCTCCGGAACAGCAAGTAAAAAATATCTTGAATGAACTCTTCCAGCGGGAA AACAGGGTATTGCATTATTGGACCATGAGGAAAAGGCGGCTGGACCAGTGTCAGCAGTACGTGGTCTTCG AGAGGAGCGCCAAGCAGGCATTAGAGTGGATCCACGACAATGGGGAGTTCTACCTTTCCACACATACCTC AACAGGGTCCAGCATTCAGCACACACAGGAGCTGTTGAAAGAACACGAGGAGTTTCAGATAACTGCCAAG CAAACCAAAGAGAGGGTGAAGCTATTGATACAGCTGGCCGATGGCTTTTGTGAAAAAGGGCATGCCCATG CAGCAGAAATTAAAAAATGTGTGACCGCAGTGGATAAGAGGTACAGAGATTTCTCTCTGCGCATGGAGAA GTACCGGACCTCTTTGGAGAAAGCCCTGGGGATTTCTTCAGATTCCAACAAATCGAGTAAAAGCCTGCAG TTGGACATCATCCCTGCCAGTATCCCCGGGTCAGAGGTGAAGCTTCGGGATGCTGCCCACGAGCTTAATG AAGAGAAGCGGAAGTCTGCCCGCAGGAAAGAGTTCATCATGGCTGAACTAATTCAAACGGAAAAGGCTTA TGTCAGAGACCTCCGAGAGTGTATGGATACCTACCTGTGGGAAATGACCAGCGGCGTGGAGGAGATCCCG CCAGGCATTGTCAACAAGGAGCTCATCATCTTCGGGAACATGCAGGAGATCTACGAGTTCCACAACAACA TCTTCCTGAAGGAGCTGGAGAAGTACGAGCAGCTCCCAGAGGACGTGGGACACTGCTTCGTCACCTGGGC AGACAAGTTTCAGATGTATGTCACATACTGCAAAAATAAACCTGATTCTACACAGCTGATACTAGAGCAC GCAGGGTCCTACTTTGATGAGATCCAGCAGCGGCATGGCTTAGCCAATTCCATCTCTTCCTACCTGATCA AACCAGTTCAGCGGATAACAAAGTATCAGCTCCTTTTAAAGGAGCTGCTCACATGCTGTGAGGAAGGAAA GGGGGAGATCAAAGATGGCCTGGAGGTGATGCTCAGCGTGCCCAAGCGAGCCAATGATGCCATGCACCTC AGCATGCTGGAAGGGTTTGACGAAAACATTGAGTCTCAGGGAGAGCTCATCCTGCAGGAGTCCTTCCAAG TGTGGGACCCGAAAACCTTAATTCGGAAGGGTCGAGAACGGCATCTCTTCCTTTTTGAAATGTCCTTGGT GTTTAGTAAAGAAGTGAAAGACTCCAGCGGGAGAAGCAAGTACCTGTACAAAAGCAAGCTGTTTACCTCA GAGTTGGGTGTCACAGAACACGTTGAAGGAGATCCTTGCAAATTTGCACTGTGGGTGGGGAGGACGCCAA CTTCAGATAATAAAATTGTCCTTAAGGCATCCAGTATCGAGAACAAGCAGGACTGGATCAAGCACATCCG TGAGGTGATCCAGGAGAGGACGGTGCACCTGAGAGGAGCCCTGAAGGAGCCCATTCACATCCCCAAAACA GCTCCGGCAGCAAGGCAGAAGGGGAGGAGGGATGGAGAAGACCTGGACAGCCAAGGGGATGGCAGCAGCC AGCCCGACACCATTTCCATCGCCTCCAGGACATCTCAGAACACACTGGACAGTGATAAGCTTTCTGGTGG CTGCGAGCTGACGGTCGTGATTCATGACTTCACAGCCTGCAACAGCAATGAGCTGACCATCCGGCGTGGC CAGACAGTGGAGGTCCTGGAACGGCCTCACGACAAGCCCGACTGGTGTCTGGTGCGCACCACTGACAGGT CCCCTGCAGCAGAAGGCCTCGTGCCCTGTGGCTCACTGTGCATCGCCCACTCCCGAAGTAGCATGGAGAT GGAGGGCATCTTCAACCACAAAGACTCACTGTCGGTCTCCAGTAATGACGCCAGCCCACCGGCCTCTGTG GCTTCCCTTCAGCCCCACATGATCGGAGCACAGAGTTCACCAGGTCCCAAGCGGCCCGGCAACACCCTGC GCAAGTGGCTCACGAGCCCCGTGCGGCGGCTCAGCAGCGGTAAGGCCGATGGGCATGCGAAGAAGCTGGC CCACAAGCACAAGAAGAGCCGGGAGGTCCGTAAGAGCGCGGATGCTGGATCGCAGAAGGATTCGGATGAC AGCGCTGCCACCCCGCAGGATGAAACCATCGAAGAGAGAGGACGGAACGAGGGTCTCAGCAGTGGTACGC TGTCCAAGTCCTCGTCCTCGGGCATGCAGAGCTGCGGAGAAGAAGAAGGCGAGGAGGGCGCCGACGCTGT ACCACTGCCTCCCCCCATGGCCATTCAGCAGCACAGCCTCCTTCAGCCGGACTCGCAGGATGACAAGGCC TCTTCAAGGTTATTGGTCCGCCCCACCAGTTCTGAGACTCCCAGCGCAGCTGAGCTCGTCAGTGCTATTG AAGAACTCGTGAAAAGCAAAATGGCACTGGAGGATCGTCCCAGCTCGCTGCTCGTCGACCAGGGAGACAG TAGCAGCCCATCCTTCAATCCCTCAGACAATTCCCTCCTCTCATCCTCCTCACCCATTGATGAGATGGAA GAAAGAAAATGCAGCTCTCTGAAGAGAAGGCACTATGTTTTGCAAGAGTTAGTGGAGACAGAGCGAGACT ATGTACGGGACCTTGGCTGTGTGGTTGAGGGCTACATGGCACTTATGAAGGAAGACGGCGTTCCTGATGA CATGAAGGGAAAAGACAAGATCGTGTTTGGCAACATCCATCAGATTTATGACTGGCACAGAGACTTTTTT TTAGGAGAGTTGGAGAAGTGCCTTGAAGATCCAGAAAAACTCGGATCCCTTTTTGTAAAGCACGAGAGAC GGTTGCACATGTACATAGTTTATTGTCAAAATAAGCCAAAGTCTGAGCACATTGTGTCCGAGTACATCGA CACCTTTTTTGAGGACTTAAAGCAGCGCCTGGGCCACAGACTGCAGCTCACTGATCTGTTGATCAAACCG GTGCAGAGAATCATGAAGTACCAGCTGCTCCTGAAGGACTTCCTCAAGTACTCAAAAAAAGCTAGCCTGG ACACATCCGAATTAGAGAAAGCTGTAGAAGTCATGTGCATCGTGCCGAAGCGCTGTAATGACATGATGAA CGTCGGGCGGCTGCAGGGATTTGACGGTAAAATTGTTGCCCAGGGTAAACTGCTCTTACAGGACACATTC CTGGTCACAGATCAAGACGCAGGACTTCTGCCTCGATGCAAAGAGAGGCGGGTTTTCCTCTTTGAACAGA TCGTCATATTCAGCGAACCACTTGATAAAAAAAAGGGCTTTTCCATGCCAGGATTTCTGTTCAAGAACAG TATCAAGGTGAGTTGCCTCTGCTTGGAGGAAAATGTGGAGAGCGATCCCTGTAAGTTCGCCCTGACGTCC AGGACGGGCGATGCCGTGGAGACTTTCGTTTTGCATTCATCCAGTCCAAGCGTCCGGCAAACGTGGATCC ATGAGATCAACCAAATCTTAGAAAACCAGCGCAACTTTTTAAACGCCTTGACATCACCCATCGAGTACCA GAGGAACCATAGTGGGGGCGGCGGCAGTGGCAGCGGGGGCAGCAGCGGCGGGGGCGGGGGCAGCGGCGGC AGCGGGGCCTCCAGCGGAGGCAGCAGCAGCCATGGCAGTGGCCCCAGCAGCTGTAGCAGTGGCCCCAGCT CGAGCAGGAGCAGGCCCTCCAGGATCCCACAGCCTGTCAGACACCACCCCCCCATGCTGGTCTCCTCTGC AGCCTCAAGCCAGGCAGAGGCAGACAAGATGTCAGGTATGTCTGCTCCCAGCCCCTCACTGCCTACCCCC AGCAGCAGCCTGGCCCTTGAGGCCAGCCTTGGCCAGCCCAGCAGGCTCCCCCTGAGTGGAGACTCCGAAG GTCATGAGCGAGAAACAGAACCCATTCCCAAGATGAAGGTGATGGAGAGCCCCCGGAAGGCCCCGGGGAG TACTTCCGGTACAAGCCAAGACGGAAACACCAAGGATGCCCGGGGAAACCTGGGCTCACTGCCTCTGGGC AAGACTAGACCTGGGGCCGTCTCACCGCTGAACTCTCCTCTCTCCACTACGTTCCCTTCTCCTTTTGGCA AGGAGGCCTTTCCTCCCAGCAGCCCCTTGCAGAAGGGGGGCTCCTTCTGGAGCTCCATTCCTGCTTCCCC TGCCAGCCGGCCAAGCTCCTTCACCTTCCCGGGGGACAGTGACTCCCTCCAGCGACAGACGCACCGCCAT GCAGCCCCCAGCAAGGACACAGACCGCATGAGCACGTGCTCCTCGGCCAGCGAGCAGTCCGTGCAGTCCA CTCAGAGCAACGGGAGTGAAGGCAGCAGCAGTAGCAACATCTCCACCATGCTGGTGACACACGAGTACAC GGCAGTGAAGGAGGATGAGATAAACGTCTATCAAGGAGAGGTCGTTCAAATTCTGGCCAGCAACCAGCAG AACATGTTTCTGGTGTTCCGAGCCGCCACTGACCAGTGCCCCGCAGCCGAGGGCTGGATTCCGGGCTTCG TCTTGGGCCATACGAGTGCAGTTATCATGGAGAACCCCGACGGGACTCTCAAGAAATCAACATCTTGGCA CACAGCACTCCGTTTAAGGAAGAAGTCTGAGAAGAAAGATAAAGACGGCAAAAGGGATGGCAAGTTGGAG AATGGGTACAGGAAGCCACGAGAAGGGCTCAGTAACAAGGTATCTGTGAAGCTTCTTAACCCCAACTACA TTTATGACGTTCCCCCAGAATTTGTTATCCCATTGAGTGAGGTCACGTGCGAGACAGGGGAGACCGTTGT TTTTAGATGTCGTGTCTGTGGCCGCCCCAAGGCCTCAATCACCTGGAAGGGCCCCGAACACAACACTTTG AACAACGATGACCACTACAGCATCTCCTACAGTGACATTGGGGAGGCCACTCTGAAGATCATTGGTGTAA GCACAGAAGATGATGGCATCTACACCTGCATCGCTGTGAATGACATGGGTTCAGCCTCATCTTCTGCCAG CCTGAGGGTTCTAGGTCCAGGGAGTGATGGGATCGTGGTGACTTGGAAGGACAACTTCGACGCCTTCTAC AGTGAAGTGGCTGAGCTTGGCAGGGGCAGGTTTGCTGTAGTTAAGAAATGTGACCAGAAGGGAACCAAAC GAGCAGTGGCCACAAAGTTTGTGAACAAGAAGTTGATGAAGCGTGACCAGGTCACTCATGAGCTCGGCAT CCTGCAGAACCTGCAGCACCCCCTCCTCGTGAGCCTTCTTGACACCTTCGAGACCCCCACCAGTTACGTC CTGGTTCTGGAAATGGCTGACCAAGGTCGCCTCCTTGACTGTGTGGTCCGGTGGGGAAGCCTCACCGAAG GGAAGGTCAGAGCGCACCTGGGGGAAGTTCTGGAAGCCGTCCGATACCTTCACAACTGCAGGATAGCACA CCTGGACCTAAAGCCTGAGAATATCTTGGTGGATCAGAGTTTGGCCAAGCCAACCATTAAACTGGCTGAC TTTGGAGACGCTGTCCAGCTCAACACCACCTACTACATCCACCAGTTGCTGGGGAACCCCGAGTTTGCAG CCCCGGAAATCATTCTTGGGAACCCTGTCTCCCTGACCGCGGACACATGGAGTGTTGGGGTGCTCACCTA CGTGCTGCTCAGCGGCGTGTCCCCCTTCCTGGATGACAGCGTGGAAGAGACCTGCCTGAATATTTGCCGG CTGGACTTTAGCTTCCCAGAGGACTACTTTCAAGGAGTTAGCCAGAAGGCCAAAGAATTCGTGTGCTTCC TCCTACAGGAGGACCCCGCCAAGCGTCCCTCGGCTGCACTGGCCCTGCAGGAACAGTGGCTGCAGGCAGG CAACGGCAGCGGCAAAGGCACGGGCGTCCTCGACACGTCCAGACTGACCTCCTTCATTGAGCGGCGCAAA CACCAGAATGATGTTCGACCTATCCGTAGCATTAAAAACTTCTTACAGAGCAGGCTTCTGCCTAGAGTTT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081302
Insert Size	9312 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081302.1, NP_001074771.1
RefSeq Size	11521 bp
RefSeq ORF	9312 bp
Locus ID	223435
UniProt ID	Q0KL02
Gene Summary	Guanine nucleotide exchange factor (GEF) for RHOA and RAC1 GTPases. Involved in coordinating actin remodeling, which is necessary for cell migration and growth (By similarity). In developing hippocampal neurons, limits dendrite formation, without affecting the establishment of axon polarity. Once dendrites are formed, involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors (AMPARs) at CA1 excitatory synapses (By similarity). May act as a regulator of adipogenesis (PubMed:22666460).[UniProtKB/Swiss-Prot Function]

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