Mki67 (NM_001081117) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D630048A14Rik; Ki-67; Ki67
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225467 representing NM_001081117 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCTCGGCTCACCTGGTCACCATCAAGCGGAGCGGCGATGACGGCGCACACTTCCCGCTGAGCC TCAGCTCCTGCCTGTTTGGAAGGAGTATTGAATGTGACATTCGTATCCAGCTGCCTGTAGTGTCAAAAAG ACATTGCAAAATTGAAGTCAAAGAGCAAGAGGCAATATTATATAATTTCAGTTCTACCAATCCAACTCAA GTAAACGGGGTTACTATAGATGAGCCTGTGAGGCTGAGACATGGAGACATAATAACCATCATTGACCGCT CCTTTAGGTATGAAGATGGAAATCATGAGGATGGAAGCAAGCCAACAGAATTTCCAGGAAAGTCCCTTGG AAAGGAACCATCAAGGCGAGCCTCAAGAGATAGCTTCTGTGCTGACCCTGATGGGGAAGGTCAAGATACC AAAGCTTCAAAAATGACTGCTTCAAGAAGATCTTTTGTGTATGCCAAGGGCCTTTCTGCAGATAGCCCTG CCTCAGATGGCTCAAAGAACAGTGTTAGCCAAGACTCATCAGGGCATGTAGAACAGCACACTGGCAGAAA CATAGTAGAGCCCACTTCTGGGGATCTCTTTAAGAAGTCCAGGTCTACAGGGAGCAGTTACAGGGAACCG AAGTCTTCTCCTACACAGAGCCTTAGCAATAGCAACGAAAAGGAATCTCCCTTTGAGAAACTTTATCAAT CAATGAAGGAAGAGTTGGATGTAAAATCCCAGAAATCTTGTAGGAAATCAGAACCCCAACCTGACCGTGC AGCAGAGGAATCGCGGGAGACACAGCTATTGGTGTCAGGCAGGGCAAGAGCAAAGTCTAGTGGAAGCACC CCTGTTACTGCAGCCTCTTCACCCAAAGTAGGAAAGATCTGGACTGAGAGATGGCGCGGTGGAATGGTGC CTGTCCAGACTTCCACAGAGACAGCTAAAATGAAGACCCCTGTGCGGCATTCACAGCAACTTAAGGATGA AGACTCTCGTGTTACTGGCAGACGACATTCTGTGAATCTGGATGAAGGTGAAAGTGCCCAGGCAGTCCAT AAAACAGTCACTCCTGGGAAACTGGCGACTAGAAACCAAGCTGCGGTGGAGGCTGGGGATGTTGCCAGCC CCGCTGATACACCAGAACATTCCTCTTCCAAAAAGAGAAGTATTCCTGCAAAGGTAGAGGCTCCATCTGC AGAGACACAAAAGCGGCTCTCTTTAACTCAGCGCCTTGTTCCAGGTGAAAAGAAAACTCCCAAGGGTTCC TTCAGCAAGCCTGAGAAACTGGCCACAGCCGCCGAACAGACTTGCTCTGGCCTACCTGGTCTTAGTTCCG TTGATATCAGCAACTTTGGTGATTCCATTAACAAGAGTGAGGGAATGCCTATGAAGAGAAGACGTGTATC CTTTGGTGGACATCTAAGACCTGAATTATTTGATGAAAACTTGCCTCCTAATACACCACTGAAAAGAGGA GAAACGCCAACCAAGAGGAAGTCTCTTGGCACTCACAGCCCAGCTGTCCTCAAGACAATCATCAAGGAAC GGCCCCAGTCTCCAGGGAAACAAGAGTCTCCTGGGATAACGCCACCGAGGACAAATGATCAAAGACGCAG ATCAGGCAGGACTTCCAGTGGAAGCAAATTCTTATGTGAGACAGACATTCCCAAGAAAGCAGGCAGGAAG AGCGGTAACCTGCCTGCGAAGAGAGCATCCATCAGCCGGAGTCAGCATGGCATTCTACAGATGATTTGCT CCAAAAGGCGAAGTGGAGCTTCTGAAGCCAACTTGATTGTTGCAAAATCATGGGCTGATGTTGTAAAACT TGGCGTGAAACAAACACAAACGAAAGTTGCGAAACATGTCCCTCAAAAGCAGACGAGCAAGAGACAAAGA AGACCCAGCACTCCAAAGAAACCCACAAGCAATCTTCACAATCAATTTACTACAGGCCATGCAAACTCTC CCTGTACCATTGTAGTAGGTAGAGCGCAGATTGAAAAAGTAAGTGTGCCTGCCCGACCCTACAAAATGCT GAATAACTTGATGCTAAACCGAAAAGTGGACTTCAGTGAAGATCTGTCAGGACTAACTGAAATGTTCAAG ACTCCAGTGAAGGAGAAGCAGCAGCAGATGAGTGATACAGGCTCCGTACTTTCCAATTCAGCGAATTTGT CTGAAAGACAATTGCAAGTAACTAATTCAGGAGACATACCTGAGCCCATCACCACAGAGATTTTGGGAGA AAAAGTGCTATCCAGTACTCGGAATGCAGCAAAGCAGCAGTCTGATAGATATTCTGCAAGTCCTACCTTA AGACGGCGGAGCATCAAACATGAAAACACAGTGCAAACTCCTAAGAATGTCCATAACATTACTGACCTTG AGAAGAAGACTCCGGTCTCTGAGACAGAGCCCCTGAAGACTGCATCGAGTGTGAGCAAGTTAAGAAGATC TAGAGAGCTCAGACATACCCTTGTGGAAACTATGAATGAAAAAACAGAAGCAGTCCTTGCTGAGAACACC ACAGCAAGACATTTAAGGGGGACATTTCGAGAACAAAAAGTAGATCAACAGGTGCAGGACAATGAAAACG CTCCTCAAAGATGCAAGGAAAGTGGTGAATTAAGTGAAGGTTCAGAAAAGACATCAGCTAGGAGATCAAG TGCCAGGAAGCAGAAGCCGACAAAAGACTTACTAGGAAGTCAGATGGTCACCCAAACAGCAGACTATGCT GAGGAACTACTTAGTCAAGGACAAGGAACCATACAAAACCTAGAGGAATCCATGCACATGCAAAACACAT CAATAAGTGAGGATCAAGGAATTACAGAAAAGAAAGTGAACATAATAGTATATGCAACCAAAGAGAAGCA CTCGCCAAAGACCCCTGGCAAAAAGGCACAACCTCTAGAAGGGCCAGCTGGTCTCAAGGAACACTTTGAA ACACCAAACCCCAAAGATAAACCTATAACGGAAGACAGAACTAGAGTCCTTTGCAAATCACCACAAGTCA CAACAGAGAATATCACAACAAACACAAAGCCACAGACTAGCACATCTGGGAAGAAAGTAGACATGAAGGA AGAAAGCTCTGCCTTGACAAAACGTATACATATGCCAGGGGAATCCAGGCATAATCCCAAAATTTTAAAA CTTGAGTGTGAGGATATCAAAGCTTTGAAGCAATCTGAAAATGAAATGCTGACCTCAACAGTAAATGGAA GCAAGAGGACTTTAGAAAAATCTAAAAAAAAGGCTCAGCCCCTGGAAGACCTGACTTGTTTCCAGGAACT CTTTATATCACCAGTTCCTACTAACATAATCAAAAAAATTCCCAGCAAATCTCCACACACACAACCAGTC AGAACCCCAGCGAGCACAAAGAGACTCTCCAAGACAGGTCTCAGTAAAGTGGATGTGAGACAAGAACCTT CAACACTTGGGAAAAGAACGAAGTCACCAGGCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAA TGACAGCACAGCCTTCATGGAAACTCCAAAGCAGAAACTGGATTTTGCAGGGAATTCATCAGGGAGTAAG AGAAGGTCACGAACATCTAAGAACAGGTCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCC AAACACCAGCTGGTGCCAGTGACTCAGTGACTGTTGAGGAAAGTGCAAAGATATCTTTGGAATCTTCACA AGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACGGTCTAAGATGAGTCTCATGAAAGTGGATATG AAAGAACTTTCCATACTTGAGAAACAAACACAGTCACGAGGCAGAGACGCAGGCACACCAGCACCAATGC AGGAAGGAAATGGCACCACAGCCATTATGGAAACTCCAAAGCAGAAACTGGATTTCACAGGAAATTCAAC AGGACATAAGAGGAGGCCACGGACACCTAAGATCAGAGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAA GAACTCTTCCAAACACCAGCTGGTGCTAATGACTCAGTGACTGTTGAGGAAAGTGCAAAGATGTCTTTGG AATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCAAGACAGATCTCAGTAA GGTGGATGTGAGAGAAGACCCTTCAATACTTGGGAAAAAAACAAAGTCACCAGGCAGAGCCCCAGGTACA CCAGCACCAGTGCAGGAAGAAAATGACTGCACAGCCTACATGGAAACTCCAAAGCAGAAACTGGAGTCTA TAGAAAATTTAACAGGGCTTAGGAAACAGTCCAGAACACCTAAAGACATCACTGGTTTCCAGGATAGTTT CCAAATACCAGATCATGCTAATGGCCCATTAGTGGTTGTCAAAACCAAAAAAATGTTCTTTAATTCTCCA CAACCAGAAAGTGCCATAACCCGAAAGAGCAGAGAGAGACAGTCTAGGGCAAGTATAAGTAAAATAGATG TTAAAGAAGAACTTTTAGAATCAGAGGAACACCTACAATTAGGAGAAGGTGTAGACACATTTCAGGTATC CACCAACAAAGTCATTAGATCATCTAGGAAACCTGCAAAGCGTAAACTGGATTCAACAGCTGGTATGCCT AACAGCAAGAGGATGCGCTGTTCTTCAAAGGATAACACACCATGCCTAGAAGACCTGAATGGCTTCCAAG AGCTCTTCCAAATGCCAGGCTATGCTAATGACTCTTTGACCACTGGAATCTCAACAATGCTTGCTAGATC ACCACAATTAGGACCAGTTAGAACCCAAATCAACAAAAAGAGTCTGCCCAAGATCATCTTGAGAAAAATG GATGTGACAGAAGAAATTTCAGGTCTCTGGAAGCAGTCACTGGGCAGAGTCCACACCACACAAGAGCAGG AGGATAATGCAATCAAAGCAATTATGGAGATTCCAAAGGAAACACTGCAGACTGCAGCAGATGGAACTAG GCTTACCAGACAGCCACAAACACCTAAGGAAAAAGTTCAACCGCTGGAAGATCACAGTGTCTTCCAAGAA CTCTTCCAAACATCACGCTACTGTTCTGATCCATTAATTGGTAACAAACAAACAAGAATGTCCTTGAGAT CTCCACAACCAGGATTTGTTAGAACTCCACGAACCTCAAAGAGACTGGCTAAGACAAGTGTTGGGAATAT TGCTGTGAGAGAAAAGATCTCTCCAGTGAGTCTGCCACAGTGTGCTACAGGGGAGGTTGTACACATACCC ATAGGGCCAGAAGATGACACAGAGAACAAAGGTGTGAAGGAATCCACACCTCAGACACTGGACTCATCAG CAAGTCGAACTGTCAGCAAGAGGCAGCAAGGGGCACATGAGGAAAGGCCTCAGTTCTCAGGAGACTTATT TCATCCCCAAGAGCTCTTTCAAACACCAGCCAGTGGCAAAGACCCAGTAACTGTTGATGAAACTACAAAA ATAGCTCTGCAGTCTCCACAACCAGGACATATCATAAACCCAGCAAGCATGAAGAGACAGTCCAACATGA GTCTCAGGAAAGACATGAGAGAATTTTCCATACTTGAAAAACAAACACAGTCACGAGGCAGAGACGCAGG CACACCAGCACCAATGCAGGAAGAAAATGGCACCACAGCCATTATGGAAACACCAAAGCAGAAACTGGAT TTCATAGGAAATTCAACAGGACATAAGAGGAGGCCTCGGACACCCAAAAACAGGGCTCAGCCCCTAGAAG ACCTGGATGGCTTCCAAGAACTCTTTCAAACACCAGCTGGTGCCAGTGACCCTGTGAGTGTTGAAGAAAG TGCAAAGATATCTTTGGCATCTTCACAAGCAGAACCAGTCAGAACCCCAGCAAGTACAAAGAGACGCTCC AAGACAGGTCTCAGTAAAGTGGATGTGAGACAAGAACCTTCAACACTTGGGAAAAGAATGAAGTCACTAG GCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACAGCACAGCCTTCATGGAAACTCCAAA GCAGAAACTGGATTTCACAGGAAATTCATCAGGACATAAGAGGAGGCCACAGACACCTAAGATCAGGGCT CAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCCAATGACTCAGTGA CTGTTGAGGAAAGTGTAAAGATGTCTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCAC AAAGAGACTCTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGACCCTTCAATACTTGAGAAAAAA ACAAAGTCACCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACTGCACAGCCTTCATGGAAACTCCAA AGCAGAAACTGGATTTCACAGGAAATTCATCAGGACATAAGAGGAGGCCACGGACACCTAAGATCAGAGC TCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCTAGTGACTCAGTG ACTGTTGAGGAAAGTGCAAAGATGTCTTTGGAATCTTCACAAGCAAAACCAGTCAAAACCCCGGCAAGCA CAAAGAGACTCTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGACCCTTCAACACTTGGGAAAAA AACAAAGTCACCAGGCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACAGCACAGCCTTC ATGGAAACTCCAAAGCAGAAACTGGATTTTGCAGAGAATTCATCAGGGAGTAAGAGAAGGTCACGAACAT CTAAGAACAGGTCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGC CAGTAACCCTGTGAGTGTTGAAGAAAGTGCAAAGATATCTTTGGAATCTTCACAAGCAGAACCAGTCAGA ACCCGGGCAAGCACAAAGAGACTTTCCAAGACAGGTCTCAATAAGATGGATGTGAGAGAAGGGCACTCTC CGCTCAGTAAGTCAAGCTGTGCATCACAGAAAGTCATGCAAACCCTCACACTTGGAGAAGATCATGGCAG AGAGACCAAAGATGGGAAGGTATTGTTAGCTCAGAAATTGGAACCAGCAATATATGTTACTCGTGGCAAG AGGCAGCAAAGGTCATGTAAGAAAAGGTCCCAGTCCCCAGAAGACCTCTCTGGTGTTCAGGAGGTCTTCC AAACATCAGGCCATAACAAGGATTCAGTGACAGTGGACAATCTTGCAAAACTGCCCAGCTCGTCTCCACC ACTAGAGCCAACAGACACTTCAGTAACCTCACGGAGACAGGCCAGAACTGGTCTGAGGAAAGTTCACGTG AAAAATGAACTTTCAGGAGGCATAATGCATCCACAAATATCAGGGGAAATTGTGGACTTACCTAGAGAAC CAGAAGGTGAAGGCAAAGTCATTAAAACAAGGAAGCAATCTGTAAAACGGAAATTGGACACAGAAGTCAA TGTGCCTCGCAGTAAGAGGCAAAGAATTACAAGAGCAGAAAAGACCCTAGAGGATCTGCCTGGCTTCCAA GAGCTCTGCCAAGCTCCAAGCTTGGTAATGGACTCAGTTATTGTTGAGAAAACCCCAAAGATGCCCGACA AATCTCCAGAACCTGTGGATACAACTTCAGAGACACAGGCAAGAAGAAGACTCAGGAGACTGGTTGTTAC TGAAGAGCCCATACCACAAAGAAAGACTACAAGAGTTGTAAGGCAAACCAGAAACACACAGAAAGAGCCC ATAAGTGACAATCAAGGTATGGAAGAGTTTAAGGAATCTTCAGTACAGAAACAAGACCCAAGTGTAAGTT TAACTGGCAGGAGGAACCAACCAAGGACAGTTAAGGAGAAAACCCAACCCTTAGAAGAACTCACCAGTTT CCAAGAGGAAACTGCCAAAAGAATATCTTCCAAATCTCCACAACCGGAAGAGAAGGAAACCTTAGCAGGT TTAAAGAGGCAGCTCAGAATACAACTAATCAACGATGGTGTAAAAGAAGAGCCCACAGCACAGAGAAAGC AACCATCCAGGGAAACCAGGAACACACTCAAAGAGCCTGTAGGTGACAGTATAAATGTTGAAGAGGTTAA GAAGTCTACAAAGCAGAAAATTGATCCAGTAGCAAGTGTGCCTGTCAGCAAGAGGCCACGGAGGGTACCC AAGGAAAAGGCACAGGCCCTAGAATTGGCTGGTCTCAAAGGACCAATCCAAACCCTAGGCCACACTGATG AATCAGCAAGTGATAAAGGACCCACACAGATGCCCTGTAATTCTCTACAACCAGAGCAAGTTGACAGCTT CCAAAGCTCACCAAGGCGACCCAGGACAAGACGTGGGAAAGTAGAGGCAGATGAAGAGCCTTCAGCAGTA AGAAAGACAGTATCAACATCAAGGCAAACTATGCGATCCCGCAAGGTCCCTGAAATTGGTAACAATGGTA CCCAAGTTTCAAAGGCCTCCATAAAGCAGACATTAGATACAGTAGCCAAAGTAACTGGCAGCAGGAGGCA GCTAAGGACACATAAGGATGGGGTTCAACCTCTTGAAGTGTTAGGTGACTCCAAAGAAATAACCCAAATA TCAGATCACTCTGAGAAACTAGCACATGACACCAGTATCCTTAAGAGCACTCAACAGCAAAAGCCAGACT CAGTAAAACCTCTGAGAACATGCAGAAGAGTGCTGAGGGCCTCTAAAGAGGACCCCAAGGAAGTGTTGGT GGACACCAGAGACCATGCAACATTACAAAGCAAAAGCAACCCTTTGCTGTCCCCGAAGAGGAAGTCTGCA AGAGATGGAAGCATTGTGAGAACCAGGGCTTTGCGCTCTTTAGCACCAAAGCAGGAAGCAACAGATGAGA AGCCTGTACCTGAGAAAAAAAGGGCTGCTTCCAGCAAGAGGCATGTATCACCTGAGCCTGTGAAGATGAA ACACCTGAAAATCGTGTCAAACAAACTTGAATCTGTGGAAGAGCAGGTTAGCACTGTTATGAAAACAGAA GAAATGGAAGCCAAAAGAGAAAATCCTGTCACTCCAGATCAGAACTCTAGGTACCGAAAGAAAACCAATG TAAAACAGCCAAGGCCCAAGTTTGATGCATCTGCAGAGAATGTCGGGATAAAGAAAAACGAGAAGACTAT GAAGACTGCCTCCCAGGAGACAGAGCTGCAGAATCCAGATGATGGAGCCAAGAAATCTACATCTCGGGGC CAAGTCAGTGGGAAAAGAACATGCTTGAGGTCTAGAGGAACGACTGAGATGCCCCAGCCTTGTGAAGCAG AAGAGAAAACAAGCAAACCAGCTGCAGAAATCTTGATAAAGCCTCAGGAAGAGAAAGGAGTCTCTGGAGA GTCTGATGTTAGGTGTTTGAGGTCCAGAAAAACTAGAGTCGCTTTGGACAGTGAACCTAAGCCAAGGGTA ACTCGTGGAACCAAGAAAGATGCAAAAACTCTGAAGGAGGATGAAGACATTGTATGCACCAAGAAGTTAA GAACAAGAAGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081117
Insert Size	9534 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081117.2, NP_001074586.2
RefSeq Size	10098 bp
RefSeq ORF	9534 bp
Locus ID	17345
UniProt ID	E9PVX6
Gene Summary	Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.[UniProtKB/Swiss-Prot Function]

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