Mki67 (NM_001081117) Mouse Untagged Clone

CAT#: MC225467

Mki67 (untagged) - Mouse antigen identified by monoclonal antibody Ki 67 (Mki67), (10ug)



  "NM_001081117" in other vectors (2)

CNY 51,020.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms D630048A14Rik; Ki-67; Ki67
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225467 representing NM_001081117
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCTCGGCTCACCTGGTCACCATCAAGCGGAGCGGCGATGACGGCGCACACTTCCCGCTGAGCC
TCAGCTCCTGCCTGTTTGGAAGGAGTATTGAATGTGACATTCGTATCCAGCTGCCTGTAGTGTCAAAAAG
ACATTGCAAAATTGAAGTCAAAGAGCAAGAGGCAATATTATATAATTTCAGTTCTACCAATCCAACTCAA
GTAAACGGGGTTACTATAGATGAGCCTGTGAGGCTGAGACATGGAGACATAATAACCATCATTGACCGCT
CCTTTAGGTATGAAGATGGAAATCATGAGGATGGAAGCAAGCCAACAGAATTTCCAGGAAAGTCCCTTGG
AAAGGAACCATCAAGGCGAGCCTCAAGAGATAGCTTCTGTGCTGACCCTGATGGGGAAGGTCAAGATACC
AAAGCTTCAAAAATGACTGCTTCAAGAAGATCTTTTGTGTATGCCAAGGGCCTTTCTGCAGATAGCCCTG
CCTCAGATGGCTCAAAGAACAGTGTTAGCCAAGACTCATCAGGGCATGTAGAACAGCACACTGGCAGAAA
CATAGTAGAGCCCACTTCTGGGGATCTCTTTAAGAAGTCCAGGTCTACAGGGAGCAGTTACAGGGAACCG
AAGTCTTCTCCTACACAGAGCCTTAGCAATAGCAACGAAAAGGAATCTCCCTTTGAGAAACTTTATCAAT
CAATGAAGGAAGAGTTGGATGTAAAATCCCAGAAATCTTGTAGGAAATCAGAACCCCAACCTGACCGTGC
AGCAGAGGAATCGCGGGAGACACAGCTATTGGTGTCAGGCAGGGCAAGAGCAAAGTCTAGTGGAAGCACC
CCTGTTACTGCAGCCTCTTCACCCAAAGTAGGAAAGATCTGGACTGAGAGATGGCGCGGTGGAATGGTGC
CTGTCCAGACTTCCACAGAGACAGCTAAAATGAAGACCCCTGTGCGGCATTCACAGCAACTTAAGGATGA
AGACTCTCGTGTTACTGGCAGACGACATTCTGTGAATCTGGATGAAGGTGAAAGTGCCCAGGCAGTCCAT
AAAACAGTCACTCCTGGGAAACTGGCGACTAGAAACCAAGCTGCGGTGGAGGCTGGGGATGTTGCCAGCC
CCGCTGATACACCAGAACATTCCTCTTCCAAAAAGAGAAGTATTCCTGCAAAGGTAGAGGCTCCATCTGC
AGAGACACAAAAGCGGCTCTCTTTAACTCAGCGCCTTGTTCCAGGTGAAAAGAAAACTCCCAAGGGTTCC
TTCAGCAAGCCTGAGAAACTGGCCACAGCCGCCGAACAGACTTGCTCTGGCCTACCTGGTCTTAGTTCCG
TTGATATCAGCAACTTTGGTGATTCCATTAACAAGAGTGAGGGAATGCCTATGAAGAGAAGACGTGTATC
CTTTGGTGGACATCTAAGACCTGAATTATTTGATGAAAACTTGCCTCCTAATACACCACTGAAAAGAGGA
GAAACGCCAACCAAGAGGAAGTCTCTTGGCACTCACAGCCCAGCTGTCCTCAAGACAATCATCAAGGAAC
GGCCCCAGTCTCCAGGGAAACAAGAGTCTCCTGGGATAACGCCACCGAGGACAAATGATCAAAGACGCAG
ATCAGGCAGGACTTCCAGTGGAAGCAAATTCTTATGTGAGACAGACATTCCCAAGAAAGCAGGCAGGAAG
AGCGGTAACCTGCCTGCGAAGAGAGCATCCATCAGCCGGAGTCAGCATGGCATTCTACAGATGATTTGCT
CCAAAAGGCGAAGTGGAGCTTCTGAAGCCAACTTGATTGTTGCAAAATCATGGGCTGATGTTGTAAAACT
TGGCGTGAAACAAACACAAACGAAAGTTGCGAAACATGTCCCTCAAAAGCAGACGAGCAAGAGACAAAGA
AGACCCAGCACTCCAAAGAAACCCACAAGCAATCTTCACAATCAATTTACTACAGGCCATGCAAACTCTC
CCTGTACCATTGTAGTAGGTAGAGCGCAGATTGAAAAAGTAAGTGTGCCTGCCCGACCCTACAAAATGCT
GAATAACTTGATGCTAAACCGAAAAGTGGACTTCAGTGAAGATCTGTCAGGACTAACTGAAATGTTCAAG
ACTCCAGTGAAGGAGAAGCAGCAGCAGATGAGTGATACAGGCTCCGTACTTTCCAATTCAGCGAATTTGT
CTGAAAGACAATTGCAAGTAACTAATTCAGGAGACATACCTGAGCCCATCACCACAGAGATTTTGGGAGA
AAAAGTGCTATCCAGTACTCGGAATGCAGCAAAGCAGCAGTCTGATAGATATTCTGCAAGTCCTACCTTA
AGACGGCGGAGCATCAAACATGAAAACACAGTGCAAACTCCTAAGAATGTCCATAACATTACTGACCTTG
AGAAGAAGACTCCGGTCTCTGAGACAGAGCCCCTGAAGACTGCATCGAGTGTGAGCAAGTTAAGAAGATC
TAGAGAGCTCAGACATACCCTTGTGGAAACTATGAATGAAAAAACAGAAGCAGTCCTTGCTGAGAACACC
ACAGCAAGACATTTAAGGGGGACATTTCGAGAACAAAAAGTAGATCAACAGGTGCAGGACAATGAAAACG
CTCCTCAAAGATGCAAGGAAAGTGGTGAATTAAGTGAAGGTTCAGAAAAGACATCAGCTAGGAGATCAAG
TGCCAGGAAGCAGAAGCCGACAAAAGACTTACTAGGAAGTCAGATGGTCACCCAAACAGCAGACTATGCT
GAGGAACTACTTAGTCAAGGACAAGGAACCATACAAAACCTAGAGGAATCCATGCACATGCAAAACACAT
CAATAAGTGAGGATCAAGGAATTACAGAAAAGAAAGTGAACATAATAGTATATGCAACCAAAGAGAAGCA
CTCGCCAAAGACCCCTGGCAAAAAGGCACAACCTCTAGAAGGGCCAGCTGGTCTCAAGGAACACTTTGAA
ACACCAAACCCCAAAGATAAACCTATAACGGAAGACAGAACTAGAGTCCTTTGCAAATCACCACAAGTCA
CAACAGAGAATATCACAACAAACACAAAGCCACAGACTAGCACATCTGGGAAGAAAGTAGACATGAAGGA
AGAAAGCTCTGCCTTGACAAAACGTATACATATGCCAGGGGAATCCAGGCATAATCCCAAAATTTTAAAA
CTTGAGTGTGAGGATATCAAAGCTTTGAAGCAATCTGAAAATGAAATGCTGACCTCAACAGTAAATGGAA
GCAAGAGGACTTTAGAAAAATCTAAAAAAAAGGCTCAGCCCCTGGAAGACCTGACTTGTTTCCAGGAACT
CTTTATATCACCAGTTCCTACTAACATAATCAAAAAAATTCCCAGCAAATCTCCACACACACAACCAGTC
AGAACCCCAGCGAGCACAAAGAGACTCTCCAAGACAGGTCTCAGTAAAGTGGATGTGAGACAAGAACCTT
CAACACTTGGGAAAAGAACGAAGTCACCAGGCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAA
TGACAGCACAGCCTTCATGGAAACTCCAAAGCAGAAACTGGATTTTGCAGGGAATTCATCAGGGAGTAAG
AGAAGGTCACGAACATCTAAGAACAGGTCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCC
AAACACCAGCTGGTGCCAGTGACTCAGTGACTGTTGAGGAAAGTGCAAAGATATCTTTGGAATCTTCACA
AGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACGGTCTAAGATGAGTCTCATGAAAGTGGATATG
AAAGAACTTTCCATACTTGAGAAACAAACACAGTCACGAGGCAGAGACGCAGGCACACCAGCACCAATGC
AGGAAGGAAATGGCACCACAGCCATTATGGAAACTCCAAAGCAGAAACTGGATTTCACAGGAAATTCAAC
AGGACATAAGAGGAGGCCACGGACACCTAAGATCAGAGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAA
GAACTCTTCCAAACACCAGCTGGTGCTAATGACTCAGTGACTGTTGAGGAAAGTGCAAAGATGTCTTTGG
AATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCACAAAGAGACTCTCCAAGACAGATCTCAGTAA
GGTGGATGTGAGAGAAGACCCTTCAATACTTGGGAAAAAAACAAAGTCACCAGGCAGAGCCCCAGGTACA
CCAGCACCAGTGCAGGAAGAAAATGACTGCACAGCCTACATGGAAACTCCAAAGCAGAAACTGGAGTCTA
TAGAAAATTTAACAGGGCTTAGGAAACAGTCCAGAACACCTAAAGACATCACTGGTTTCCAGGATAGTTT
CCAAATACCAGATCATGCTAATGGCCCATTAGTGGTTGTCAAAACCAAAAAAATGTTCTTTAATTCTCCA
CAACCAGAAAGTGCCATAACCCGAAAGAGCAGAGAGAGACAGTCTAGGGCAAGTATAAGTAAAATAGATG
TTAAAGAAGAACTTTTAGAATCAGAGGAACACCTACAATTAGGAGAAGGTGTAGACACATTTCAGGTATC
CACCAACAAAGTCATTAGATCATCTAGGAAACCTGCAAAGCGTAAACTGGATTCAACAGCTGGTATGCCT
AACAGCAAGAGGATGCGCTGTTCTTCAAAGGATAACACACCATGCCTAGAAGACCTGAATGGCTTCCAAG
AGCTCTTCCAAATGCCAGGCTATGCTAATGACTCTTTGACCACTGGAATCTCAACAATGCTTGCTAGATC
ACCACAATTAGGACCAGTTAGAACCCAAATCAACAAAAAGAGTCTGCCCAAGATCATCTTGAGAAAAATG
GATGTGACAGAAGAAATTTCAGGTCTCTGGAAGCAGTCACTGGGCAGAGTCCACACCACACAAGAGCAGG
AGGATAATGCAATCAAAGCAATTATGGAGATTCCAAAGGAAACACTGCAGACTGCAGCAGATGGAACTAG
GCTTACCAGACAGCCACAAACACCTAAGGAAAAAGTTCAACCGCTGGAAGATCACAGTGTCTTCCAAGAA
CTCTTCCAAACATCACGCTACTGTTCTGATCCATTAATTGGTAACAAACAAACAAGAATGTCCTTGAGAT
CTCCACAACCAGGATTTGTTAGAACTCCACGAACCTCAAAGAGACTGGCTAAGACAAGTGTTGGGAATAT
TGCTGTGAGAGAAAAGATCTCTCCAGTGAGTCTGCCACAGTGTGCTACAGGGGAGGTTGTACACATACCC
ATAGGGCCAGAAGATGACACAGAGAACAAAGGTGTGAAGGAATCCACACCTCAGACACTGGACTCATCAG
CAAGTCGAACTGTCAGCAAGAGGCAGCAAGGGGCACATGAGGAAAGGCCTCAGTTCTCAGGAGACTTATT
TCATCCCCAAGAGCTCTTTCAAACACCAGCCAGTGGCAAAGACCCAGTAACTGTTGATGAAACTACAAAA
ATAGCTCTGCAGTCTCCACAACCAGGACATATCATAAACCCAGCAAGCATGAAGAGACAGTCCAACATGA
GTCTCAGGAAAGACATGAGAGAATTTTCCATACTTGAAAAACAAACACAGTCACGAGGCAGAGACGCAGG
CACACCAGCACCAATGCAGGAAGAAAATGGCACCACAGCCATTATGGAAACACCAAAGCAGAAACTGGAT
TTCATAGGAAATTCAACAGGACATAAGAGGAGGCCTCGGACACCCAAAAACAGGGCTCAGCCCCTAGAAG
ACCTGGATGGCTTCCAAGAACTCTTTCAAACACCAGCTGGTGCCAGTGACCCTGTGAGTGTTGAAGAAAG
TGCAAAGATATCTTTGGCATCTTCACAAGCAGAACCAGTCAGAACCCCAGCAAGTACAAAGAGACGCTCC
AAGACAGGTCTCAGTAAAGTGGATGTGAGACAAGAACCTTCAACACTTGGGAAAAGAATGAAGTCACTAG
GCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACAGCACAGCCTTCATGGAAACTCCAAA
GCAGAAACTGGATTTCACAGGAAATTCATCAGGACATAAGAGGAGGCCACAGACACCTAAGATCAGGGCT
CAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCCAATGACTCAGTGA
CTGTTGAGGAAAGTGTAAAGATGTCTTTGGAATCTTCACAAGCAGAACCAGTCAAAACCCCGGCAAGCAC
AAAGAGACTCTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGACCCTTCAATACTTGAGAAAAAA
ACAAAGTCACCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACTGCACAGCCTTCATGGAAACTCCAA
AGCAGAAACTGGATTTCACAGGAAATTCATCAGGACATAAGAGGAGGCCACGGACACCTAAGATCAGAGC
TCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCTAGTGACTCAGTG
ACTGTTGAGGAAAGTGCAAAGATGTCTTTGGAATCTTCACAAGCAAAACCAGTCAAAACCCCGGCAAGCA
CAAAGAGACTCTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGACCCTTCAACACTTGGGAAAAA
AACAAAGTCACCAGGCAGAGCCCCAGGCACACCAGCACCAGTGCAGGAAGAAAATGACAGCACAGCCTTC
ATGGAAACTCCAAAGCAGAAACTGGATTTTGCAGAGAATTCATCAGGGAGTAAGAGAAGGTCACGAACAT
CTAAGAACAGGTCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGC
CAGTAACCCTGTGAGTGTTGAAGAAAGTGCAAAGATATCTTTGGAATCTTCACAAGCAGAACCAGTCAGA
ACCCGGGCAAGCACAAAGAGACTTTCCAAGACAGGTCTCAATAAGATGGATGTGAGAGAAGGGCACTCTC
CGCTCAGTAAGTCAAGCTGTGCATCACAGAAAGTCATGCAAACCCTCACACTTGGAGAAGATCATGGCAG
AGAGACCAAAGATGGGAAGGTATTGTTAGCTCAGAAATTGGAACCAGCAATATATGTTACTCGTGGCAAG
AGGCAGCAAAGGTCATGTAAGAAAAGGTCCCAGTCCCCAGAAGACCTCTCTGGTGTTCAGGAGGTCTTCC
AAACATCAGGCCATAACAAGGATTCAGTGACAGTGGACAATCTTGCAAAACTGCCCAGCTCGTCTCCACC
ACTAGAGCCAACAGACACTTCAGTAACCTCACGGAGACAGGCCAGAACTGGTCTGAGGAAAGTTCACGTG
AAAAATGAACTTTCAGGAGGCATAATGCATCCACAAATATCAGGGGAAATTGTGGACTTACCTAGAGAAC
CAGAAGGTGAAGGCAAAGTCATTAAAACAAGGAAGCAATCTGTAAAACGGAAATTGGACACAGAAGTCAA
TGTGCCTCGCAGTAAGAGGCAAAGAATTACAAGAGCAGAAAAGACCCTAGAGGATCTGCCTGGCTTCCAA
GAGCTCTGCCAAGCTCCAAGCTTGGTAATGGACTCAGTTATTGTTGAGAAAACCCCAAAGATGCCCGACA
AATCTCCAGAACCTGTGGATACAACTTCAGAGACACAGGCAAGAAGAAGACTCAGGAGACTGGTTGTTAC
TGAAGAGCCCATACCACAAAGAAAGACTACAAGAGTTGTAAGGCAAACCAGAAACACACAGAAAGAGCCC
ATAAGTGACAATCAAGGTATGGAAGAGTTTAAGGAATCTTCAGTACAGAAACAAGACCCAAGTGTAAGTT
TAACTGGCAGGAGGAACCAACCAAGGACAGTTAAGGAGAAAACCCAACCCTTAGAAGAACTCACCAGTTT
CCAAGAGGAAACTGCCAAAAGAATATCTTCCAAATCTCCACAACCGGAAGAGAAGGAAACCTTAGCAGGT
TTAAAGAGGCAGCTCAGAATACAACTAATCAACGATGGTGTAAAAGAAGAGCCCACAGCACAGAGAAAGC
AACCATCCAGGGAAACCAGGAACACACTCAAAGAGCCTGTAGGTGACAGTATAAATGTTGAAGAGGTTAA
GAAGTCTACAAAGCAGAAAATTGATCCAGTAGCAAGTGTGCCTGTCAGCAAGAGGCCACGGAGGGTACCC
AAGGAAAAGGCACAGGCCCTAGAATTGGCTGGTCTCAAAGGACCAATCCAAACCCTAGGCCACACTGATG
AATCAGCAAGTGATAAAGGACCCACACAGATGCCCTGTAATTCTCTACAACCAGAGCAAGTTGACAGCTT
CCAAAGCTCACCAAGGCGACCCAGGACAAGACGTGGGAAAGTAGAGGCAGATGAAGAGCCTTCAGCAGTA
AGAAAGACAGTATCAACATCAAGGCAAACTATGCGATCCCGCAAGGTCCCTGAAATTGGTAACAATGGTA
CCCAAGTTTCAAAGGCCTCCATAAAGCAGACATTAGATACAGTAGCCAAAGTAACTGGCAGCAGGAGGCA
GCTAAGGACACATAAGGATGGGGTTCAACCTCTTGAAGTGTTAGGTGACTCCAAAGAAATAACCCAAATA
TCAGATCACTCTGAGAAACTAGCACATGACACCAGTATCCTTAAGAGCACTCAACAGCAAAAGCCAGACT
CAGTAAAACCTCTGAGAACATGCAGAAGAGTGCTGAGGGCCTCTAAAGAGGACCCCAAGGAAGTGTTGGT
GGACACCAGAGACCATGCAACATTACAAAGCAAAAGCAACCCTTTGCTGTCCCCGAAGAGGAAGTCTGCA
AGAGATGGAAGCATTGTGAGAACCAGGGCTTTGCGCTCTTTAGCACCAAAGCAGGAAGCAACAGATGAGA
AGCCTGTACCTGAGAAAAAAAGGGCTGCTTCCAGCAAGAGGCATGTATCACCTGAGCCTGTGAAGATGAA
ACACCTGAAAATCGTGTCAAACAAACTTGAATCTGTGGAAGAGCAGGTTAGCACTGTTATGAAAACAGAA
GAAATGGAAGCCAAAAGAGAAAATCCTGTCACTCCAGATCAGAACTCTAGGTACCGAAAGAAAACCAATG
TAAAACAGCCAAGGCCCAAGTTTGATGCATCTGCAGAGAATGTCGGGATAAAGAAAAACGAGAAGACTAT
GAAGACTGCCTCCCAGGAGACAGAGCTGCAGAATCCAGATGATGGAGCCAAGAAATCTACATCTCGGGGC
CAAGTCAGTGGGAAAAGAACATGCTTGAGGTCTAGAGGAACGACTGAGATGCCCCAGCCTTGTGAAGCAG
AAGAGAAAACAAGCAAACCAGCTGCAGAAATCTTGATAAAGCCTCAGGAAGAGAAAGGAGTCTCTGGAGA
GTCTGATGTTAGGTGTTTGAGGTCCAGAAAAACTAGAGTCGCTTTGGACAGTGAACCTAAGCCAAGGGTA
ACTCGTGGAACCAAGAAAGATGCAAAAACTCTGAAGGAGGATGAAGACATTGTATGCACCAAGAAGTTAA
GAACAAGAAGTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001081117
Insert Size 9534 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081117.2, NP_001074586.2
RefSeq Size 10098 bp
RefSeq ORF 9534 bp
Locus ID 17345
UniProt ID E9PVX6
Gene Summary Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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