Tafa5 (NM_001252310) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Fam19; Fam19a5; Tafa-5; Tar; Tara-5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225818 representing NM_001252310 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCATCGCCCAGGACCAGCAGCCGGCAAGATGCGACCGCCCTGCCCAGCATGTCCTCAACTTTTT GGGCATTCATGATCCTGGCCAGCCTGCTCATCGCCTACTGCAGTCAGCTGGCCGCTGGAACCTGTGAGAT TGTGACCCTAGACCGGGACAGCAGCCAGCCACGGAGGACGATCGCCCGGCAGACAGCACGCTGTGCATGC AGAAAGGGGCAGATAGCAGGCACCACTCGAGCCCGGCCTGCTTGTGTGGACGCTCGAATTATCAAGACAA AGCAGTGGTGTGACATGCTTCCTTGCCTGGAGGGGGAAGGCTGTGACTTGTTAATCAACCGGTCAGGCTG GACTTGCACACAGCCCGGAGGGCGGATAAAGACCACCACGGTCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252310
Insert Size	399 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252310.1, NP_001239239.1
RefSeq Size	2665 bp
RefSeq ORF	399 bp
Locus ID	106014
UniProt ID	Q91WE9
Gene Summary	This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines, that act as regulators of immune and nervous cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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