Marchf8 (NM_001302383) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300017E09Rik; M; Marc; MARCH-VIII; March8; Mir
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226796 representing NM_001302383 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATGCCATTGCACCAGATCTCTGCCATCCCTTCTCAGGATGCCATTTCTGCTAGAGTCTACAGAA GCAAGACCAAAGATAAGGAGCAGAATGAGAAGACTTTGGGACATTCCATGAGTCATCCAAGCAACATTTC TAAGGCTGGGAGTAGTCCTCCATCCACGACGGCTCCAGTGTCTGCCTTCTCTCGCACTTCTGTCACACCA TCCAACCAGGACATCTGCAGGATCTGCCACTGTGAAGGGGATGACGAGAGCCCTCTGATCACCCCCTGTC ACTGCACAGGGAGCCTCCATTTCGTGCATCAGGCTTGCCTGCAGCAGTGGATCAAGAGTTCTGACACACG CTGCTGTGAACTCTGCAAGTACGAGTTCATCATGGAGACCAAGCTGAAACCTTTGAGGAAATGGGAGAAG TTGCAGATGACTGCCAGTGAGCGCAGGAAGATCATGTGCTCAGTGACCTTCCATGTCATTGCTATCACCT GTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGCACAGCAGAGGAAATCAAGCAGGGTCAGGTAACAGG AATCCTAGAGTGGCCTTTCTGGACGAAGCTGGTAGTTGTGGCCATCGGCTTCACTGGAGGACTTCTCTTT ATGTATGTTCAGTGCAAGGTGTACCTACAGTTATGGAAAAGACTCAAGGCTTACAATAGAGTGATCTATG TTCAGAACTGTCCAGAAACAAGTAAAAAGAATATTTTTGAAAAGTCTGCACTTACAGAGCCCACCCTTGA AAATAAAGAAGGACATGGAATGTGTCATTCCACCACAAATTCTTCTTGCACAGAGCCTGAAGACACTGGA GCAGAAATTATTAACGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302383
Insert Size	861 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302383.1, NP_001289312.1
RefSeq Size	4484 bp
RefSeq ORF	861 bp
Locus ID	71779
UniProt ID	Q9DBD2
Gene Summary	The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).

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