Grk6 (NM_001286066) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gprk6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226860 representing NM_001286066 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGGTGACCTAAAGTTCCACATCTACCACATGGGCCAGGCTGGCTTTCCTGAAGCACGTGCTG TCTTCTATGCTGCTGAGATCTGCTGTGGCCTGGAAGACCTGCACCGGGAACGCATTGTGTACAGGGATCT AAAGCCAGAGAATATCCTTCTGGATGACCATGGCCACATTCGGATCTCGGACCTGGGACTGGCCGTGCAT GTGCCTGAGGGCCAGACCATCAAAGGCCGTGTGGGCACTGTGGGCTACATGGCTCCAGAGGTGGTGAGGA ATGAGCGCTACACGTTCAGTCCTGACTGGTGGGCGCTAGGCTGCCTCCTGTACGAGATGATCGCAGGTCA GTCGCCCTTCCAGCAGAGGAAGAAGAAGATAAAGCGCGAAGAGGTGGAGCGGCTGGTCAAGGAAGTGGCC GAGGAGTACACAGACCGCTTTTCCTCACAGGCGCGCTCACTCTGTTCTCAGCTTCTTAGCAAGGACCCTG CTGAGCGCCTGGGGTGTCGTGGAGGTGGCGCCCGTGAGGTAAAGGAGCACCCCCTTTTCAAGAAACTGAA TTTCAAGCGGCTGGGAGCTGGCATGCTAGAGCCACCTTTTAAGCCTGATCCCCAGGCTATTTATTGCAAG GATGTCCTGGACATTGAACAGTTCTCTACAGTTAAAGGTGTGGATCTGGAGCCCACAGACCAAGACTTCT ACCAGAAGTTTGCCACAGGCAGTGTGTCCATCCCCTGGCAGAATGAGATGGTGGAGACCGAGTGCTTCCA GGAACTCAATGTCTTTGGGCTGGATGGGTCTGTTCCCCCAGACCTGGACTGGAAGGGCCAGCCCACTGCA CCCCCCAAGAAGGGATTGCTACAGAGACTCTTCAGTCGCCAAAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286066
Insert Size	888 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286066.1, NP_001272995.1
RefSeq Size	2093 bp
RefSeq ORF	888 bp
Locus ID	26385
Gene Summary	Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) has multiple differences compared to variant 1. These differences result in a distinct 5' UTR, 3' UTR, and 3' coding region, and cause translation initiation from a downstream start codon. The encoded isoform (g) has a shorter N-terminus and a distinct and shorter C-terminus, compared to isoform a.

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