Nanog (NM_001289828) Mouse Untagged Clone
CAT#: MC226915
Nanog (untagged) - Mouse Nanog homeobox (Nanog), transcript variant 2
CNY 2,950.00
货期*
3周
规格
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经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 2410002E02Rik; ecat; ecat4; EN; ENK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC226915 representing NM_001289828
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTGGGTCTTCCTGGTCCCCACAGTTTGCCTAGTTCTGAGGAAGCATCGAATTCTGGGAACGCCT CATCAATGCCTGCAGTTTTTCATCCCGAGAACTATTCTTGCTTACAAGGGTCTGCTACTGAGATGCTCTG CACAGAGGCTGCCTCTCCTCGCCCTTCCTCTGAAGACCTGCCTCTTCAAGGCAGCCCTGATTCTTCTACC AGTCCCAAACAAAAGCTCTCAAGTCCTGAGGCTGACAAGGGCCCTGAGGAGGAGGAGAACAAGGTCCTTG CCAGGAAGCAGAAGATGCGGACTGTGTTCTCTCAGGCCCAGCTGTGTGCACTCAAGGACAGGTTTCAGAA GCAGAAGTACCTCAGCCTCCAGCAGATGCAAGAACTCTCCTCCATTCTGAACCTGAGCTATAAGCAGGTT AAGACCTGGTTTCAAAACCAAAGGATGAAGTGCAAGCGGTGGCAGAAAAACCAGTGGTTGAAGACTAGCA ATGGTCTGATTCAGGGCTCAGCACCAGTGGAGTATCCCAGCATCCATTGCAGCTATCCCCAGGGCTATCT GGTGAACGCATCTGGAAGCCTTTCCATGTGGGGCAGCCAGACTTGGACCAACCCAACTTGGAGCAGCCAG ACCTGGACCAACCCAACTTGGAACAACCAGACCTGGACCAACCCAACTTGGAGCAGCCAGGCCTGGACCG CTCAGTCCTGGAACGGCCAGCCTTGGAATGCTGCTCCGCTCCATAACTTCGGGGAGGACTTTCTGCAGCC TTACGTACAGTTGCAGCAAAACTTCTCTGCCAGTGATTTGGAGGTGAATTTGGAAGCCACTAGGGAAAGC CATGCGCATTTTAGCACCCCACAAGCCTTGGAATTATTCCTGAACTACTCTGTGACTCCACCAGGTGAAA TATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001289828 |
Insert Size | 915 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001289828.1, NP_001276757.1 |
RefSeq Size | 2220 bp |
RefSeq ORF | 915 bp |
Locus ID | 71950 |
UniProt ID | Q80Z64 |
Gene Summary | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR229126 | Nanog (myc-DDK-tagged) - Mouse Nanog homeobox (Nanog), transcript variant 2 |
CNY 3,040.00 |
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