Stk16 (NM_001277992) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	EDPK; Krct; MPSK; PKL12; TSF-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226918 representing NM_001277992 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCACGCACTGTGTGTCTGCTCCCGGGGAACTGTCATCATTGACAATAAGCGTTACCTCTTCGTCC AGAAATTGGGGGAAGGTGGATTCAGCTATGTGGACCTAGTGGAGGGCTTGCATGATGGACACTTCTACGC CCTGAAGCGGATCCTGTGCCATGAGCAGCAAGACCAGGAAGAAGCCCAACGAGAGGCAGAGATGCATCGC CTCTTCCAGCATCCCAACATCCTTCGCCTCATGGCTTACTCTCTGAAAGAACGAGGTGCTAAGCATGAAG CCTGGCTGCTGCTGCCCTTCTTCAAGAAAGGTACACTGTGGAATGAGATAGAAAGGCTGAAGGACCAAGG CAGCTTCCTGACTGAAGACCAGATCCTGCCGCTGTTGCTGGGTATCAGCAGAGGCCTTGAGGCTATTCAT GCCAAAGGTTATGCACACAGGGACCTGAAGCCCACCAATATTTTGCTTGGTGATGAGGGGCAGCCAGTTT TAATGGACTTGGGTTCTATGAATCAAGCATGCATTCAAGTGGAGGGCTCTCGCCAGGCACTAGCTCTTCA GGACTGGGCAGCTCAGCGGTGCACCATCTCCTACCGGGCACCTGAACTTTTTTCTGTGCAAAGCCACTGT GTCATCGATGAGCGGACTGATGTCTGGTCCCTAGGCTGTGTGCTTTATGCCATGATGTTTGGGGAAGGCC CTTACGATATGGTGTTCCAGAAGGGTGACAGTGTGGCCCTTGCTGTGCAGAATGAACTCAGCATCCCACA AAGCCCCAGGCATTCTTCAGCATTGCGACAGCTATTGTCTTCTATGATGACTGTGGACCCCCAGCAGAGG CCTCACATCCCTGTCCTCCTCAGTCAGTTGGAGGCATTGCAGCCACCAGCTCCTGGCCAGCACACCACCC AAATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277992
Insert Size	918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277992.1, NP_001264921.1
RefSeq Size	2857 bp
RefSeq ORF	918 bp
Locus ID	20872
UniProt ID	O88697
Gene Summary	Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates (By similarity). May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.

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