Abhd4 (NM_001205181) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110035H23Rik; Abh4; AI429574
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226999 representing NM_001205181 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAACTGAAGAATGTGGAAGCCAGGATCCTCCAGTGTCTCCAGAACAAGTTCCTGGCCCGTTATG TATCCCTCCCAAACCAGAACAAGATCTGGACGGTGACTGTGAGCCCAGAACAAAAGGATCGCACCCCTCT GGTGATGGTACATGGCTTTGGGGGCGGTGTGGGCCTCTGGATCCTCAACATGGATTCACTGAGTGCCCGC CGCACACTTCATACCTTTGATCTGCTTGGTTTTGGGCGAAGCTCAAGGCCAACATTCCCAAGGGACCCAG AAGGAGCTGAAGATGAGTTTGTGGCCTCAATAGAGACATGGCGGGAGACCATGGGAATCCCCACCATGAT CCTCCTGGGGCACAGTTTGGGAGGATTCCTGGCCACTTCTTACTCTATCAAGTACCCTGAAAGAGTTAAA CATCTTATCCTGGTGGATCCATGGGGCTTTCCCCTACGACCAACTGACCCTAGTGAGATCCGTGCACCTC CAACCTGGGTCAAGGCTGTGGCATCTGTCCTGGGACGTTCCAATCCACTGGCTGTTCTTCGAGTGGCTGG GCCTTGGGGGCCTGGGCTGGTGCAGAGATTCCGTCCAGACTTCAAGCGCAAGTTTGCAGACTTCTTTGAG GATGACACCATCTCGGAATACATCTACCACTGCAATGCACAGAATCCCAGTGGGGAAACGGCATTCAAAG CCATGATGGAGTCCTTTGGCTGGGCCCGGCGCCCCATGTTGGAGCGAATCCACTTAATTCGAAAAGATGT GCCCATCACCATGATCTATGGGGCCAACACCTGGATAGATACCAGCACAGGGAAGAAGGTGAAGATGCAA AGGCCGGATTCCTACGTCCGAGACATGGAGATCGAGGGCGCATCCCACCACGTCTATGCTGACCAGCCAC ACATCTTCAATGCTGTGGTAGAAGAGATCTGCAACTCAGTTGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205181
Insert Size	957 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205181.1, NP_001192110.1
RefSeq Size	2661 bp
RefSeq ORF	957 bp
Locus ID	105501
UniProt ID	Q8VD66
Gene Summary	Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis (PubMed:16818490, PubMed:25853435). Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains (PubMed:16818490, PubMed:25853435). Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (PubMed:16818490).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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