Hs3st5 (NM_001253356) Mouse Untagged Clone
CAT#: MC227188
Hs3st5 (untagged) - Mouse heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (Hs3st5), transcript variant 2
CNY 3,330.00
货期*
3周
规格
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | D930005L05Rik; Gm1151; Hs3ost5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC227188 representing NM_001253356
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTATTCAAACAGCAGGTGTGGCTGAGACAGAAGCTCCTGGTACTGGGAAGCCTTGCTGTTGGGAGCC TCCTGTATCTAGTTGCCAGAGTTGGGAGCTTGGATAGGCTCCAGCCCATTTGCCCTGTTGAAAGTCGATT TGGTGGTGCCCACAACCAGGCTGAGTTGCCACTGCGGGCCCTGCAGTTTAAGAGAGGCTTGCTGCATGAG TTCCGGAAGGGCAATTCTTCCAAGGAGCAGGTTCACCTCCATGACCTGGTCCAACAGCTCCCCAAAGCCA TTATCATTGGGGTGAGAAAAGGGGGCACAAGGGCCCTGCTAGAGATGCTCAACCTCCATCCTGCAGTGGT CAAAGCTTCCCAAGAGATCCACTTCTTTGACAATGATGAGAATTATGCCAAGGGCATTGAGTGGTACAGG AAAAAGATGCCTTTTTCCTACCCTCAGCAAATCACGATTGAAAAGAGCCCGGCATATTTCATCACAGAAG AGGTTCCGGAAAGGATTTACAAGATGAACTCATCCATCAAGCTGTTGATCATTGTCAGGGAGCCGACCAC AAGAGCAATTTCTGATTATACTCAGGTGCTAGAGGGGAAGGAGCGGAAGAACAAAACCTACTATAAGTTT GAAAAACTGGCTATAGACCCTAATACCTGTGAAGTGAACACGAAATACAAGGCGGTTAGGACCAGCATAT ACACAAAACATCTGGAGCGCTGGTTGAAATACTTTCCCATTGAACAGTTTCATATCGTAGATGGTGACCG TCTTATCACAGAACCTCTGCCGGAACTACAGCTGGTGGAAAAGTTCTTAAACCTTCCTCCGAGGATAAGT CAGTACAATTTATATTTCAATGCTACCAGAGGGTTTTACTGTCTGAGATTTAACATTATCTTTAATAAGT GCCTGGCGGGCAGCAAGGGGCGCATCCATCCAGAGGTAGACCCCTCCGTCATTACCAAATTGCGCAAATT CTTTCATCCTTTTAATCAAAAATTTTACCAGATCACTGGGAGGACATTGAACTGGCCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001253356 |
Insert Size | 1041 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001253356.1, NP_001240285.1 |
RefSeq Size | 3063 bp |
RefSeq ORF | 1041 bp |
Locus ID | 319415 |
UniProt ID | Q8BSL4 |
Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Also generates GlcUA-GlcNS or IdoUA-GlcNS and IdoUA2S-GlcNH2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR229399 | Hs3st5 (myc-DDK-tagged) - Mouse heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (Hs3st5), transcript variant 2 |
CNY 3,656.00 |
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