Arntl2 (NM_001289681) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4632430A05Rik; bHLHe6; BMAL2; CLIF; MOP9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227245 representing NM_001289681 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGCTGTACCGTCCCTGTCAAAGAAGAGCAGCCATGCTCGTCCTGCTCAAAGAAGAAAGACCATA GAAAATTCCACACCGTCCATTGCACTGGATACTTGAGAAGCTGGCCTCTGAATGTTGTTGGCATGGAGAA AGAGTCGGGTGGTGGGAAGGACAGCGGTCCTCTTACCTGCCTTGTGGCTATGGGACGGTTGCATCCATAC ATTGTCCCTCAAAAGAGTGGCAAGATCAACGTGAGACCGGCTGAGTTCATAACTCGCTTCGCAATGAACG GGAAATTCGTCTATGTTGACCAAAGGGCAACGGCAATTTTAGGATACCTGCCTCAGGAACTTTTGGGAAC TTCATGTTATGAATATTTTCATCAGGATGACCACAGTAGTTTGACTGACAAGCACAAAGCAGTTCTGCAG AGTAAGGAGAAAATACTTACAGACTCATACAAATTCAGAGTGAAGGATGGTGCCTTCGTGACTCTGAAGA GTGAGTGGTTCAGCTTCACAAACCCTTGGACCAAAGAGCTGGAGTACATTGTGTCTGTCAACACATTGGT TTTGGGGCGCAGTGAGACCAGGCTGTCTTTGCTTCATTGCGGCGGCAGCAGCCAGTCCTCCGAAGACTCA TTTAGACAATCCTGCATCAATGTGCCCGGTGTATCCACGGGGACCGTCCTTGGTGCTGGGAGTATTGGAA CAGATATTGCAAATGAGGTTCTGAGTTTACAGAGATTACACTCTTCATCCCCAGAAGATGCAAGCCCTTC AGAAGAAGTGAGAGATGACTGCAGTGTAAATGGTGGGAATGCCTATGGGCCTGCATCCACTAGGGAGCCT TTTGCAGTGAGCCCTTCTGAAACAGAGGTCCTGGAGGCTGCCAGGCAACACCAGAGCACTGAACCCGCCC ACCCTCACGGACCACTTCCCGGTGACAGTGCCCAGCTGGGTTTTGATGTCCTGTGTGACAGTGACAGCAT AGACATGGCTGCATTCATGAATTACCTCGAAGCAGAGGGGGGCCTGGGTGACCCTGGGGACTTCAGTGAC ATCCAGTGGGCACTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289681
Insert Size	1068 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289681.1, NP_001276610.1
RefSeq Size	2547 bp
RefSeq ORF	1068 bp
Locus ID	272322
UniProt ID	Q2VPD4
Gene Summary	This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (Per, Arnt, Sim) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian Clock protein, the structurally related Mop4, and hypoxia-inducible factors, such as Hif1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (4) uses an alternate splice site in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus than isoform 1.

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