Wdr45 (NM_001290792) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C79260; DXImx38e; JM5; Sfc19; Wdrx1; WIPI-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227272 representing NM_001290792 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCAGCAGCCACTTCGAGGTGTGACCAGCCTACATTTCAACCAAGACCAAAGCTGCTTTTGCTGCG CCATGGAGACAGGCGTCCGGATCTACAATGTGGAGCCACTTATGGAGAAGGGGCATCTTGACCACGAGCA GGTAGGCAGCGTGGGCCTGGTAGAGATGCTGCACCGATCCAACCTGCTGGCCCTGGTGGGCGGTGGGAGC AGCCCCAAGTTCTCTGAGATCTCAGTGCTGATCTGGGACGATGCCCGAGAAGGCAAGGACTCCAAGGACA AACTGGTGCTGGAGTTCACCTTCACCAAGCCAGTGCTGGCTGTGCGCATGCGCCATGACAAGATCGTGAT TGTGCTGAGGAACCGCATCTATGTGTACTCCTTCCCTGACAGTCCAAGAAAGCTGTTTGAGTTTGACACT CGGGACAACCCCAAGGGGCTGTGTGACCTCTGTCCAAGCCTGGAGAAGCAGCTGCTCGTGTTTCCTGGAC ACAAGTGTGGAAGTCTGCAACTTGTGGATCTCGCAAGCACAAAGCCTGGTACTTCGTCGGCGCCATTCAC TATCAATGCACATCAGAGTGATGTGGCCTGTGTGTCCCTGAACCAGCCAGGCACTGTAGTGGCGTCAGCC TCCCAGAAGGGCACCCTTATTCGTCTCTTTGATACCCAATCCAAGGAAAAGCTGGTAGAGCTTCGAAGAG GCACCGACCCTGCCACCCTGTACTGCATTAACTTCAGCCACGACTCCTCCTTCCTCTGTGCTTCCAGTGA CAAGGGCACTGTCCACATCTTCGCTCTTAAAGACACCCGCCTTAACCGCCGCTCTGCGCTGGCTCGTGTG GGCAAAGTGGGACCTATGATTGGGCAATACGTGGACTCTCAGTGGAGCCTGGCCAGCTTTACTGTGCCTG CTGAGTCAGCCTGCATCTGCGCCTTTGGTCGAAATACTTCCAAGAATGTCAATTCTGTAATTGCCATCTG TGTAGATGGGACCTTCCACAAATATGTCTTCACTCCTGATGGAAACTGCAACAGAGAGGCCTTTGACGTG TACCTTGACATCTGTGATGACGAGGACTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290792
Insert Size	1083 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290792.1, NP_001277721.1
RefSeq Size	1601 bp
RefSeq ORF	1083 bp
Locus ID	54636
UniProt ID	Q91VM3
Gene Summary	Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Probably recruited to membranes through its PtdIns3P activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 encode isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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