Vsx2 (NM_001301427) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Chx10; Hox-1; Hox-10; Hox1; Hox10; or
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227395 representing NM_001301427 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGGAAAGCGGGGGAAGCGCTGAGCAAGCCCAAATCCGAGACAGTGGCCAAGAGTACCTCGGGGG GCGCCCCGGCCAGGTGCACAGGGTTCGGCATCCAGGAGATCCTAGGCTTGAACAAGGAGCCTCCCAGCTC CCACCCGCGGGCTGCCCTCGACGGCCTGGCCCCTGGGCATTTGTTAGCTGCGCGCTCCGTTCTCAGCCCG GCAGGAGTGGGCAGCATGGGGCTGCTGGGGCCCGGGGGACTTCCCGGCTTCTACACACAGCCCACCTTCT TGGAAGTGCTGTCCGATCCGCAGAGCGTCCACTTGCAGCCACTAGGCAGAGCGTCGGGGCCGCTGGACAC CAGCCAGACGGCCAGCTCCGATTCCGAAGATGTTTCCTCCAGTGACCGAAAAATGTCCAAATCGGCTTTA AACCAGACCAAGAAGCGTAAGAAGCGGCGACACAGGACAATCTTTACTTCCTACCAGCTAGAGGAGCTGG AGAAAGCATTCAATGAAGCCCACTACCCAGATGTCTACGCCCGGGAGATGCTGGCCATGAAAACGGAGCT CCCAGAAGACAGGATACAGGTGTGGTTCCAGAACCGCAGAGCCAAGTGGAGGAAGAGGGAGAAGTGCTGG GGCCGGAGCAGCGTCATGGCTGAGTATGGTCTCTATGGAGCCATGGTGAGGCACTCGATCCCCCTGCCAG AGTCTATCCTCAAGTCTGCCAAGGATGGCATAATGGATTCCTGTGCCCCATGGCTACTGGTTCAAGATGG CTTTCCCAGGCGCTTTTCTAAACCCGAATACCAACAATTCTTTTTAGGGATGCACAAAAAGTCGCTGGAG GCAGCAGCTGAGTCGGGAAGGAAGCCCGAGGTGGAACGCCAGGCCCTGCCCAAGCTCGACAAGATGGAGC AGGAGGAACGGGCCCCAGAGGCCCAGGCAGCCATCTCCCAGGAAGAACTGAGGGAGAACAGCATTGCCGC CCTCCGAGCCAAAGCTCAGGAACACAGCACCAAGGTTCTGGGGACTGTATCTGGGCCTGACAGCCTGGCC AGAAATGCTGAGAAGCCAGAAGAAGAGGACGCCACGGAGGAAGACAGGCCAGCTGAGAAGCTCAGCCCAC CGCAGCTGGAGGACATGGCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301427
Insert Size	1143 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301427.1, NP_001288356.1
RefSeq Size	3276 bp
RefSeq ORF	1143 bp
Locus ID	12677
Gene Summary	This gene encodes a member of the Vsx (visual system homeobox) family which belongs to the larger PRD homeobox class. The encoded protein is required for eye organogenesis and controls retinal development. Disruption of this gene is associated with ocular retardation J (orJ), a mouse disease which causes microphthalmia, retinal degeneration and optic nerve aplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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