Ager (NM_001271423) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	RAGE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227423 representing NM_001271423 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCGGGGACAGCAGCTAGAGCCTGGGTGCTGGTTCTTGCTCTATGGGGAGCTGTAGCTGGTGGTC AGAACATCACAGCCCGGATTGGAGAGCCACTTGTGCTAAGCTGTAAGGGGGCCCCTAAGAAGCCGCCCCA GCAGCTAGAATGGAAACTGGTCCTCTCTCCCCAGGGAGGCCCCTGGGACAGCGTGGCTCGAATCCTCCCC AATGGTTCCCTCCTCCTTCCAGCCACTGGAATTGTCGATGAGGGGACTTTCCGGTGTCGGGCAACTAACA GGCGAGGGAAGGAGGTCAAGTCCAACTACCGAGTCCGAGTCTACCAGATTCCTGGGAAGCCAGAAATTGT GGATCCTGCCTCTGAACTCACAGCCAGTGTCCCTAATAAGGTGGGGACATGTGTGTCTGAGGGAAGCTAC CCTGCAGGGACCCTTAGCTGGCACTTAGATGGGAAACTTCTGATTCCCGATGGCAAAGAAACACTCGTGA AGGAAGAGACCAGGAGACACCCTGAGACGGGACTCTTTACACTGCGGTCAGAGCTGACAGTGATCCCCAC CCAAGGAGGAACCCATCCTACCTTCTCCTGCAGTTTCAGCCTGGGCCTTCCCCGGCGCAGACCCCTGAAC ACAGCCCCCATCCAACTCCGAGTCAGGGAGCCTGGGCCTCCAGAGGGCATTCAGCTGTTGGTTGAGCCTG AAGGTGGAATAGTCGCTCCTGGTGGGACTGTGACCTTGACCTGTGCCATCTCTGCCCAGCCCCCTCCTCA GGTCCACTGGATAAAGGATGGTGCACCCTTGCCCCTGGCTCCCAGCCCTGTGCTGCTCCTCCCTGAGGTG GGGCACGAGGATGAGGGCACCTATAGCTGCGTGGCCACCCACCCTAGCCACGGACCTCAGGAAAGCCCTC CTGTCAGCATCAGGGTCACAGGCTCTGTGGGTGAGTCTGGGCTGGGTACGCTAGCCCTGGCCTTGGGGAT CCTGGGAGGCCTGGGAGTAGTAGCCCTGCTCGTCGGGGCTATCCTGTGGCGAAAACGACAACCCAGGCGT GAGGAGAGGAAGGCCCCGGAAAGCCAGGAGGATGAGGAGGAACGTGCAGAGCTGAATCAGTCAGAGGAAG CGGAGATGCCAGAGAATGGTGCCGGGGGACCGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271423
Insert Size	1155 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271423.1, NP_001258352.1
RefSeq Size	1333 bp
RefSeq ORF	1155 bp
Locus ID	11596
UniProt ID	Q62151
Gene Summary	Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site and lacks an in-frame exon, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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